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1. 发明申请

WO2004006655A3 NUCLEAR FERTILITY RESTORER GENES AND METHODS OF USE IN PLANTS 审中-公开
标题翻译：核能恢复基因及其在植物中的使用方法
公开(公告)号：WO2004006655A3
公开(公告)日：2004-04-08
申请号：PCT/US0301343
申请日：2003-01-16
申请人： UNIV MCGILL , DNA LANDMARKS INC , BASF PLANT SCIENCE GMBH , BROWN GREGORY G , FORMANOVA NATASA , DENDY CHARLES , LANDRY BENOIT S , CHEUNG WING , JIN HUA , LAI FANG MING , LEFOREST MARTIN
发明人： BROWN GREGORY G , FORMANOVA NATASA , DENDY CHARLES , LANDRY BENOIT S , CHEUNG WING , JIN HUA , LAI FANG MING , LEFOREST MARTIN
IPC分类号： C07K14/415 , C12N15/10 , C12N15/29 , C12N15/82 , A01H1/02 , A01H5/00
CPC分类号： C12N15/8287 , C07K14/415 , C12N15/1034 , C12N15/821 , C12N15/8289
摘要： The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in Brassica napus.
摘要翻译：本发明包括核能恢复基因，由这些基因编码的蛋白质和转基因植物以及含有这些基因的植物细胞。更具体地说，核生育恢复基因可用于恢复细胞质雄性不育植物如甘蓝型油菜中的生育力。优选地，核生育恢复基因与欧洲油菜的Ogura（ogu）CMS系统一起使用。

2. 发明申请

WO2004006655A8 NUCLEAR FERTILITY RESTORER GENES AND METHODS OF USE IN PLANTS 审中-公开
标题翻译：核能恢复基因及其在植物中的使用方法
公开(公告)号：WO2004006655A8
公开(公告)日：2005-02-03
申请号：PCT/US0301343
申请日：2003-01-16
申请人： UNIV MCGILL , DNA LANDMARKS INC , BASF PLANT SCIENCE GMBH , BROWN GREGORY G , FORMANOVA NATASA , DENDY CHARLES , LANDRY BENOIT S , CHEUNG WING , JIN HUA , LAI FANG MING , LAFOREST MARTIN
发明人： BROWN GREGORY G , FORMANOVA NATASA , DENDY CHARLES , LANDRY BENOIT S , CHEUNG WING , JIN HUA , LAI FANG MING , LAFOREST MARTIN
IPC分类号： C07K14/415 , C12N15/10 , C12N15/29 , C12N15/82 , A01H1/02 , A01H5/00
CPC分类号： C12N15/8287 , C07K14/415 , C12N15/1034 , C12N15/821 , C12N15/8289
摘要： The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in Brassica napus.
摘要翻译：本发明包括核能恢复基因，由这些基因编码的蛋白质和转基因植物以及含有这些基因的植物细胞。更具体地说，核生育恢复基因可用于恢复细胞质雄性不育植物如甘蓝型油菜中的生育力。优选地，核生育恢复基因与欧洲油菜的Ogura（ogu）CMS系统一起使用。

3. 发明申请

WO0212551A3 MAP-BASED GENOME MINING METHOD FOR IDENTIFYING GENE REGULATORY LOCI 审中-公开
标题翻译：基于MAP的基因组挖掘方法，用于鉴定基因调控位点
公开(公告)号：WO0212551A3
公开(公告)日：2003-10-02
申请号：PCT/CA0101028
申请日：2001-07-17
申请人： CANADA NATURAL RESOURCES , DNA LANDMARKS INC , CASTONGUAY YVES , O'DONOUGHUE LOUISE S , LANDRY BENOIT S , LABERGE SERGE , MONROY ANTONIO F , VEZINA LOUIS P
发明人： CASTONGUAY YVES , O'DONOUGHUE LOUISE S , LANDRY BENOIT S , LABERGE SERGE , MONROY ANTONIO F , VEZINA LOUIS P
IPC分类号： A01H1/00 , C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6897 , C12Q1/6809
摘要： The invention pertains to a method for identifying one or more regions within a genome of an organism of interest that mediate the expression of one or more genes of interest. The method comprises identifying a first and a second organism of interest, the first organism of interest is characterized by exhibiting a measurable response to an environmental stimulus, or otherwise exhibiting a phenotype associated with differential gene expression associated with a process of interest. The second organism of interest is characterized by lacking or not exhibit as strong a response to the stimulus as that observed within the first organism of interest, or it exhibits a different phenotype compared with that of the first organism of interest, wherein the different phenotype is associated with the process of interest, or it exhibits a phenotype of interest that segregates when compared with the first organism of interest, or a combination thereof. The first and second organisms of interest are crossed to produce a population of segregated progeny and RNA is extracted from each of the segregated progeny. The level of gene expression for one or more genes of interest that are associated with the response to an environmental stimulus, or process of interest is quantified.
摘要翻译：本发明涉及用于鉴定介导一种或多种感兴趣基因表达的感兴趣生物体的基因组内的一个或多个区域的方法。该方法包括鉴定感兴趣的第一和第二生物体，所述第一感兴趣的生物体的特征在于显示对环境刺激的可测量的反应，或者表现出与感兴趣的过程相关的差异基因表达相关的表型。第二有兴趣的生物体的特征在于缺乏或不表现出与目标第一生物体内观察到的对刺激的强烈反应，或者与第一感兴趣的生物体相比表现出不同的表型，其中不同的表型是或者与感兴趣的第一生物体相比，其表现出分离的感兴趣的表型，或其组合。将感兴趣的第一和第二生物杂交以产生分离后代的群体，并且从每个分离的后代中提取RNA。与感兴趣的环境刺激或感兴趣过程相关的一种或多种感兴趣的基因的基因表达水平被量化。

4. 发明申请

WO2004018639A2 MALE STERILITY RESTORATION AS A SELECTABLE MARKER IN PLANT TRANSFORMATION 审中-公开
标题翻译：作为植物转化中的可选标记的雄性不育恢复
公开(公告)号：WO2004018639A2
公开(公告)日：2004-03-04
申请号：PCT/US2003/026593
申请日：2003-08-25
申请人： BASF PLANT SCIENCE GMBH , DNA LANDMARKS, INC. , LAI, Fang-Ming , LANDRY, Benoit, S.
发明人： LAI, Fang-Ming , LANDRY, Benoit, S.
IPC分类号： C12N
CPC分类号： C12N15/8289 , C12N15/821
摘要： The present invention provides methods of identifying transformed plants using the restoration of male fertility as a selectable marker. Preferably, the fertility restorer genes are used to restore fertility in Brassica napus. The present invention also includes vectors comprising male sterility genes flanked by recombinase sites, vectors comprising a fertility restorer gene and a nucleotide sequence of interest flanked by recombinase sites, and methods of using such vectors to produce transgenic plants, using restoration of male fertility as the selection for transformation events.
摘要翻译：本发明提供了使用恢复雄性能育性作为选择标记来鉴定转化植物的方法。优选地，用育性恢复基因来恢复欧洲油菜的育性。本发明还包括包含侧接重组酶位点的雄性不育基因的载体，包含生育力恢复基因的载体和侧接重组酶位点的感兴趣的核苷酸序列，以及使用这种载体产生转基因植物的方法，男性生育力作为转化事件的选择。

5. 发明申请

WO2003006622A3 NUCLEAR FERTILITY RESTORER GENES AND METHODS OF USE IN PLANTS 审中-公开
公开(公告)号：WO2003006622A3
公开(公告)日：2003-01-23
申请号：PCT/US2002/022217
申请日：2002-07-12
申请人： MCGILL UNIVERSITY , DNA LANDMARKS, INC. , BROWN, Gregory, G. , FORMANOVA, Natasa , DENDY, Charles , LANDRY, Benoit, S. , CHEUNG, Wing , JIN, Hua
发明人： BROWN, Gregory, G. , FORMANOVA, Natasa , DENDY, Charles , LANDRY, Benoit, S. , CHEUNG, Wing , JIN, Hua
IPC分类号： C12N5/04
摘要： The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus . Preferably, the nuclear fertility restorer genes are used with the Ogura ( ogu ) CMS system in Brassica napus .

6. 发明申请

WO2004090160A1 METHODS FOR RELATIVE QUANTIFICATION OF SPECIFIC NUCLEIC ACID SEQUENCES 审中-公开
标题翻译：特定核酸序列相对量化方法
公开(公告)号：WO2004090160A1
公开(公告)日：2004-10-21
申请号：PCT/CA2003/000547
申请日：2003-04-11
申请人： DNA LANDMARKS INC. , LAFOREST, Martin , HUBERT, Nathalie , LANDRY, Benoît, S.
发明人： LAFOREST, Martin , HUBERT, Nathalie , LANDRY, Benoît, S.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/686 , C12Q2565/301 , C12Q2545/114 , C12Q2545/101
摘要： This invention relates generally to a method for quantifying the number of occurrences of a specific nucleic acid sequence within a nucleic acid sample in order to circumvent the shortcomings of the methods currently available and to provide reliable quantification of a specific nucleic acid sequence within a nucleic acid sample. The present invention provides a method of assessing an amount of a known target nucleic acid sequence in a sample comprising co-amplifying said target nucleic acid sequence and a known amount of a known control nucleic acid sequence to produce respective target and control amplicons, wherein said control nucleic acid sequence is different than said target nucleic acid sequence; and determining relative amounts of said respective amplicons by determining relative quantities of a primer extension reaction using each of said respective amplicons as a template.
摘要翻译：本发明一般涉及用于量化核酸样品中特定核酸序列的出现次数的方法，以便规避目前可获得的方法的缺点并提供核酸内的特定核酸序列的可靠定量样品。本发明提供了一种评估样品中已知靶核酸序列的量的方法，包括共扩增所述靶核酸序列和已知量的已知对照核酸序列以产生相应的靶和扩增子，其中所述对照核酸序列与所述靶核酸序列不同; 以及通过使用每个所述各个扩增子作为模板来确定引物延伸反应的相对量来确定所述各个扩增子的相对量。

7. 发明申请

WO2004018639A3 MALE STERILITY RESTORATION AS A SELECTABLE MARKER IN PLANT TRANSFORMATION 审中-公开
标题翻译：作为植物转化中的可选标记的男性不育恢复
公开(公告)号：WO2004018639A3
公开(公告)日：2004-04-15
申请号：PCT/US0326593
申请日：2003-08-25
申请人： BASF PLANT SCIENCE GMBH , DNA LANDMARKS INC , LAI FANG-MING , LANDRY BENOIT S
发明人： LAI FANG-MING , LANDRY BENOIT S
IPC分类号： C12N15/31 , C12N15/55 , C12N15/82 , A01H1/02
CPC分类号： C12N15/8289 , C12N15/821
摘要： The present invention provides methods of identifying transformed plants using the restoration of male fertility as a selectable marker. Preferably, the fertility restorer genes are used to restore fertility in Brassica napus. The present invention also includes vectors comprising male sterility genes flanked by recombinase sites, vectors comprising a fertility restorer gene and a nucleotide sequence of interest flanked by recombinase sites, and methods of using such vectors to produce transgenic plants, using restoration of male fertility as the selection for transformation events.
摘要翻译：本发明提供使用雄性育性恢复作为选择性标记鉴定转化植物的方法。优选地，使用育性恢复基因来恢复甘蓝型油菜的生育力。本发明还包括包含侧翼为重组酶位点的雄性不育基因的载体，包含生育恢复基因的载体和与重组酶位点相关的目的核苷酸序列，以及使用这些载体产生转基因植物的方法，使用恢复雄性生育力作为选择转型事件。

8. 发明申请

WO2004006655A2 NUCLEAR FERTILITY RESTORER GENES AND METHODS OF USE IN PLANTS 审中-公开
标题翻译：核生殖恢复基因及其在植物中的使用方法
公开(公告)号：WO2004006655A2
公开(公告)日：2004-01-22
申请号：PCT/US2003/001343
申请日：2003-01-16
申请人： MCGILL UNIVERSITY , DNA LANDMARKS, INC. , BASF PLANT SCIENCE GMBH , BROWN, Gregory, G. , FORMANOVA, Natasa , DENY, Charles , LANDRY, Benoit, S. , CHEUNG, Wing , JIN, Hua , LAI, Fang, Ming , LEFOREST, Martin
发明人： BROWN, Gregory, G. , FORMANOVA, Natasa , DENY, Charles , LANDRY, Benoit, S. , CHEUNG, Wing , JIN, Hua , LAI, Fang, Ming , LEFOREST, Martin
IPC分类号： A01H
CPC分类号： C12N15/8287 , C07K14/415 , C12N15/1034 , C12N15/821 , C12N15/8289
摘要： The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus . Preferably, the nuclear fertility restorer genes are used with the Ogura ( ogu ) CMS system in Brassica napus .
摘要翻译：本发明包括核生育力恢复基因，由这些基因编码的蛋白质和转基因植物以及含有这些基因的植物细胞。更具体地说，核雄性不育恢复基因可用于恢复细胞质雄性不育植物如甘蓝型油菜的育性。优选地，核转化恢复基因与欧洲油菜中的Ogura（ogu）CMS系统一起使用。

9. 发明申请

WO2003006622A2 NUCLEAR FERTILITY RESTORER GENES AND METHODS OF USE IN PLANTS 审中-公开
标题翻译：核能恢复基因及其在植物中的使用方法
公开(公告)号：WO2003006622A2
公开(公告)日：2003-01-23
申请号：PCT/US2002/022217
申请日：2002-07-12
申请人： MCGILL UNIVERSITY , DNA LANDMARKS, INC. , BROWN, Gregory, G. , FORMANOVA, Natasa , DENDY, Charles , LANDRY, Benoit, S. , CHEUNG, Wing , JIN, Hua
发明人： BROWN, Gregory, G. , FORMANOVA, Natasa , DENDY, Charles , LANDRY, Benoit, S. , CHEUNG, Wing , JIN, Hua
IPC分类号： C12N
CPC分类号： C12N15/1034 , C07K14/415 , C12N15/821 , C12N15/8287 , C12N15/8289
摘要： The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus . Preferably, the nuclear fertility restorer genes are used with the Ogura ( ogu ) CMS system in Brassica napus .
摘要翻译：本发明包括核能恢复基因，由这些基因编码的蛋白质和转基因植物以及含有这些基因的植物细胞。更具体地说，核生育恢复基因可用于恢复细胞质雄性不育植物如甘蓝型油菜中的生育力。优选地，核生育恢复基因与欧洲油菜的Ogura（ogu）CMS系统一起使用。

10. 发明申请

WO0060113A3 TRANSPOSON-BASED GENETIC MARKER 审中-公开
标题翻译：基于传统的遗传标记
公开(公告)号：WO0060113A3
公开(公告)日：2001-04-05
申请号：PCT/CA0000351
申请日：2000-03-30
申请人： UNIV MCGILL , DNA LANDMARKS INC , LANDRY BENOIT , THOMAS BUREAU , CHANG RUYING , DONOUGHUE LOUISE STEPHANIE O
发明人： BUREAU THOMAS , CHANG RUYING , O'DONOUGHUE LOUISE STEPHANIE
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6827
摘要： The present invention relates to the use of DNA primers homologous to MITE in a method for detecting polymorphisms of a nucleic acid sequence. The method comprises the steps of amplifying nucleic acid sequences using a first primer homologous to a MITE in combination with another primer homologous or not to a MITE, separating fragments of the nucleic acid sequences amplified, and analyzing the fragments obtained in relation to reference fragments obtained from amplification of a nucleic acid sequence with the primer homologous to MITE for determining polymorphism in the nucleic acid sequence. DNA primers homologous to MITE may also be used in genotyping, fingerprinting, mapping or cloning method in accordance with the invention.
摘要翻译：本发明涉及在检测核酸序列多态性的方法中使用与MITE同源的DNA引物。该方法包括以下步骤：使用与MITE同源的第一引物与与MITE同源的另一个引物组合扩增核酸序列，分离扩增的核酸序列的片段，并分析与获得的参考片段相关的片段从具有与MITE同源的引物的核酸序列的扩增用于确定核酸序列中的多态性。与MITE同源的DNA引物也可用于根据本发明的基因分型，指纹图谱，映射或克隆方法中。

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