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1. 发明申请

WO2004018639A2 MALE STERILITY RESTORATION AS A SELECTABLE MARKER IN PLANT TRANSFORMATION 审中-公开
标题翻译：作为植物转化中的可选标记的雄性不育恢复
公开(公告)号：WO2004018639A2
公开(公告)日：2004-03-04
申请号：PCT/US2003/026593
申请日：2003-08-25
申请人： BASF PLANT SCIENCE GMBH , DNA LANDMARKS, INC. , LAI, Fang-Ming , LANDRY, Benoit, S.
发明人： LAI, Fang-Ming , LANDRY, Benoit, S.
IPC分类号： C12N
CPC分类号： C12N15/8289 , C12N15/821
摘要： The present invention provides methods of identifying transformed plants using the restoration of male fertility as a selectable marker. Preferably, the fertility restorer genes are used to restore fertility in Brassica napus. The present invention also includes vectors comprising male sterility genes flanked by recombinase sites, vectors comprising a fertility restorer gene and a nucleotide sequence of interest flanked by recombinase sites, and methods of using such vectors to produce transgenic plants, using restoration of male fertility as the selection for transformation events.
摘要翻译：本发明提供了使用恢复雄性能育性作为选择标记来鉴定转化植物的方法。优选地，用育性恢复基因来恢复欧洲油菜的育性。本发明还包括包含侧接重组酶位点的雄性不育基因的载体，包含生育力恢复基因的载体和侧接重组酶位点的感兴趣的核苷酸序列，以及使用这种载体产生转基因植物的方法，男性生育力作为转化事件的选择。

2. 发明申请

WO2008145731A1 METHOD OF EXCISING A NUCLEIC ACID SEQUENCE FROM A PLANT GENOME 审中-公开
标题翻译：从植物基因组中提取核酸序列的方法
公开(公告)号：WO2008145731A1
公开(公告)日：2008-12-04
申请号：PCT/EP2008/056691
申请日：2008-05-30
申请人： BASF PLANT SCIENCE GMBH , SONG, Hee-Sook , LAI, Fang-Ming , ROCHE, Christina E. , BROWN, Jeffrey A.
发明人： SONG, Hee-Sook , LAI, Fang-Ming , ROCHE, Christina E. , BROWN, Jeffrey A.
IPC分类号： C12N15/82 , A01H5/00 , C12N9/22
CPC分类号： C12N15/8201 , C12N9/22
摘要： The present invention relates to a method for excising a nucleic acid sequence from the genome of a plant or a plant cell. This method is based on the steps of transforming a plant cell with a construct encoding a DNA double strand break inducingenzyme (DSBI), generating a transgenic plant line, performing a transient assay to analyze the functionality of the transgenic enzyme, crossing the plant line with a line containing a nucleic acid sequence to be excised and performing an immature embryo conversion or a tissue culture regeneration through callus formation. The method can also be reversed, which means that a plant cell is transformed with a construct encoding a nucleic acid sequence to be excised, and the crossing is performed witha plant line containing a DSBI. As an alternative to the crossing step, a re-transformation of a transgenic plant line with a second construct can also be performed. The invention is also directed to a plant obtained by this method, or progeny, propagation material, part, tissue, cell or cell culture, derived from such a plant. Finally, the invention relates to the use of a plant or progeny, propagation material, part, tissue, cell or cell culture, derived from this method, as aliment, fodder or seeds or for the production of pharmaceuticals or chemicals.
摘要翻译：本发明涉及从植物或植物细胞的基因组中切除核酸序列的方法。该方法基于用编码DNA双链断裂诱导酶（DSBI）的构建体转化植物细胞的步骤，产生转基因植物系，进行瞬时测定以分析转基因酶的功能，穿过植物品系与包含要切除的核酸序列并通过愈伤组织形成进行未成熟胚转化或组织培养再生的线。该方法也可以逆转，这意味着用编码要切除的核酸序列的构建体转化植物细胞，并且用包含DSBI的植物系进行杂交。作为交叉步骤的替代方案，也可以进行具有第二构建体的转基因植物系的再转化。本发明还涉及通过该方法获得的植物，或从这种植物衍生的后代，繁殖材料，部分，组织，细胞或细胞培养物。最后，本发明涉及来源于该方法的植物或后代，繁殖材料，部分，组织，细胞或细胞培养物作为营养物，饲料或种子或用于生产药物或化学品的用途。

3. 发明申请

WO2007014844A2 SELECTION SYSTEM FOR MAIZE 审中-公开
标题翻译：选择系统
公开(公告)号：WO2007014844A2
公开(公告)日：2007-02-08
申请号：PCT/EP2006/064356
申请日：2006-07-18
申请人： BASF PLANT SCIENCE GMBH , LAI, Fang-Ming , MANKIN, Luke , MEI, Kangfeng , JONES, Todd , SONG, Hee-Sook
发明人： LAI, Fang-Ming , MANKIN, Luke , MEI, Kangfeng , JONES, Todd , SONG, Hee-Sook
IPC分类号： C12N15/82
CPC分类号： C12N15/821
摘要： The present invention relates to improved methods for the incorporation of DNA into the genome of a Zea mays plant based on a D-alanine or D-serine selection. Preferably the transformation is mediated by Agrobacterium.
摘要翻译：本发明涉及基于D-丙氨酸或D-丝氨酸选择将DNA并入玉米基因组的基因组中的改进方法。优选转化由农杆菌介导。

4. 发明申请

WO2006136596A3 IMPROVED METHODS FOR THE PRODUCTION OF STABLY TRANSFORMED, FERTILE ZEA MAYS PLANTS 审中-公开
公开(公告)号：WO2006136596A3
公开(公告)日：2006-12-28
申请号：PCT/EP2006/063448
申请日：2006-06-22
申请人： BASF PLANT SCIENCE GMBH , PENG, Jianying , MRABET, Penny , BAGLEY, Christopher , WANG, Zhongqi , MANKIN, Luke , SIGNH, Bijay , LAI, Fang-Ming , MEI, Kangfeng , ZHANG, Hongyi , NEA, Larry
发明人： PENG, Jianying , MRABET, Penny , BAGLEY, Christopher , WANG, Zhongqi , MANKIN, Luke , SIGNH, Bijay , LAI, Fang-Ming , MEI, Kangfeng , ZHANG, Hongyi , NEA, Larry
IPC分类号： C12N15/82 , A01H5/10
摘要： The present invention relates to improved methods for the incorporation of DNA into the genome of a Zea mays plant by means of Agrobacterium -mediated transformation. Preferred is the use of the Zea may lines deposited with American Type Culture Collection under the Patent Deposit Designation PTA-6170 and PTA-6171.

5. 发明申请

WO2010146046A1 HYDROPONIC SYSTEMS FOR GENERATING TRANSGENIC PLANTS 审中-公开
标题翻译：用于产生转基因植物的水文系统
公开(公告)号：WO2010146046A1
公开(公告)日：2010-12-23
申请号：PCT/EP2010/058377
申请日：2010-06-15
申请人： BASF PLANT SCIENCE COMPANY GMBH , JONES, Todd , OLHOFT, Paula , VANGURI, Aparna Sri , LAI, Fang-Ming , KUIJPERS, Anne-Marie , GEIGER, Michael
发明人： JONES, Todd , OLHOFT, Paula , VANGURI, Aparna Sri , LAI, Fang-Ming , KUIJPERS, Anne-Marie , GEIGER, Michael
IPC分类号： A01H1/04 , C12N15/82 , A01G31/00
CPC分类号： C12N15/8201 , A01G31/00 , A01H1/04
摘要： The present invention is concerned with a method for generating transgenic plants, said method comprising a) providing a population of transgenic and non-transgenic rootless plant shoots grown under in vitro conditions, transferring said plant shoots to a hydroponic medium, and simultaneously allowing said shoots to root on said hydroponic medium and selecting for transgenic shoots.
摘要翻译：本发明涉及用于产生转基因植物的方法，所述方法包括：a）提供在体外条件下生长的转基因和非转基因无根植物芽的群体，将所述植物芽转移到水培培养基中，同时允许所述芽根据所述水培培养基并选择转基因芽。

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