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    • 4. 发明申请
    • A FAMILY OF PESTICIDAL PROTEINS AND METHODS FOR THEIR USE
    • 一种农药蛋白质及其使用方法
    • WO2007147096A3
    • 2008-07-24
    • PCT/US2007071298
    • 2007-06-15
    • ATHENIX CORPKOZIEL MICHAEL GDESAI NALINIDETER REBEKAHTOMSO DANIEL JOHNHARGISS TRACYDUCK NICHOLAS BCAROZZI NADINEGUO RONG
    • KOZIEL MICHAEL GDESAI NALINIDETER REBEKAHTOMSO DANIEL JOHNHARGISS TRACYDUCK NICHOLAS BCAROZZI NADINEGUO RONG
    • C07K14/195C07K14/32C12N15/82
    • C12N15/8286A01N37/44C07K14/325C12P21/02Y02A40/162
    • Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.
    • 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所述的核苷酸序列,所述核苷酸序列如SEQ ID NO:1,3,5,8,10,12,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号NRRL B-30961,B-30955,B-30956,B-30957,B-30958,B -30942,B-30939,B-30941,B-50047,B-50047,B-30959,B-30960,B-30943,B-50048或B-50048,以及它们的变体和片段。
    • 5. 发明申请
    • A FAMILY OF PESTICIDAL PROTEINS AND METHODS FOR THEIR USE
    • 一种农药蛋白质及其使用方法
    • WO2007147096A2
    • 2007-12-21
    • PCT/US2007/071298
    • 2007-06-15
    • ATHENIX CORPORATIONKOZIEL, Michael, G.DESAI, NaliniDETER, RebekahTOMSO, Daniel, JohnHARGISS, TracyDUCK, Nicholas, B.CAROZZI, NadineGUO, Rong
    • KOZIEL, Michael, G.DESAI, NaliniDETER, RebekahTOMSO, Daniel, JohnHARGISS, TracyDUCK, Nicholas, B.CAROZZI, NadineGUO, Rong
    • C07K14/43
    • C12N15/8286A01N37/44C07K14/325C12P21/02Y02A40/162
    • Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.
    • 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所述的核苷酸序列,所述核苷酸序列如SEQ ID NO:1,3,5,8,10,12,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号NRRL B-30961,B-30955,B-30956,B-30957,B-30958,B -30942,B-30939,B-30941,B-50047,B-50047,B-30959,B-30960,B-30943,B-50048或B-50048,以及它们的变体和片段。
    • 7. 发明申请
    • AXMI115 VARIANT INSECTICIDAL GENE AND METHODS FOR ITS USE
    • AXMI115变种杀虫剂基因及其使用方法
    • WO2012138703A1
    • 2012-10-11
    • PCT/US2012/032086
    • 2012-04-04
    • ATHENIX CORP.LEHTINEN, DuaneDESAI, Nalini, ManojHEINRICHS, Volker
    • LEHTINEN, DuaneDESAI, Nalini, ManojHEINRICHS, Volker
    • C12N15/82C07K14/325A01H5/00
    • C12N15/8286A01N37/44A01N63/02C07K14/32C07K14/325C07K2319/00C07K2319/24C12N15/8279C12N15/8285Y02A40/162
    • Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:48 and 15-31, including biologically active variants and fragments thereof.
    • 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 毒素编码序列可用于DNA构建体或表达盒中用于在植物和细菌中表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了多核苷酸序列和由此编码的毒素蛋白质。 还提供了特异性结合那些氨基酸序列的抗体。 特别地,本发明包括编码融合蛋白的核苷酸序列,以及其生物活性变体及其片段,其中融合蛋白含有SEQ ID NO:43的C-末端部分。 融合蛋白还可以含有SEQ ID NO:45的N-末端部分。 本发明还包括SEQ ID NO:47和1-14的核苷酸序列,或编码SEQ ID NO:48和15-31所示氨基酸序列的核苷酸序列,包括生物活性变体和片段。
    • 9. 发明申请
    • SYNTHETIC AXMI-004 DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE
    • 合成AXMI-004三角内毒素基因及其使用方法
    • WO2009036234A1
    • 2009-03-19
    • PCT/US2008/076104
    • 2008-09-12
    • ATHENIX CORPORATIONCAROZZI, NadineDESAI, NaliniBEILINSON, VadimTOMSO, Daniel, J.KAHN, Theodore
    • CAROZZI, NadineDESAI, NaliniBEILINSON, VadimTOMSO, Daniel, J.KAHN, Theodore
    • C07K14/325C12N15/82
    • C07K14/325C12N15/8286Y02A40/162
    • Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided, particularly synthetically- derived coding sequences. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, or 18, or the nucleotide sequence set forth in SEQ ID NO: 1, 2, 4, 6, 7, 8, 10, 12, 14, 16, or 17, as well as variants and fragments thereof.
    • 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供包含δ-内毒素多肽的编码序列的组合物,特别是合成衍生的编码序列。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列,以及与这些氨基酸序列特异性结合的抗体。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO:9,11,13,15或18所示氨基酸序列的核苷酸序列或SEQ ID NO:1所示的核苷酸序列, 2,4,6,7,8,10,12,14,16或17,以及其变体和片段。