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1. 发明申请

WO1995008000A2 REAGENT AND METHOD FOR THE DETECTION OF A NUCLEOTIDE SEQUENCE WITH SIGNAL AMPLIFICATION 审中-公开
标题翻译：用于检测具有信号放大的核苷酸序列的试剂和方法
公开(公告)号：WO1995008000A2
公开(公告)日：1995-03-23
申请号：PCT/FR1994001084
申请日：1994-09-15
申请人： BIO MERIEUX , MANDRAND, Bernard , CROS, Philippe , DELAIR, Thierry , CHARLES, Marie-Hélène , EROUT, Marie-Noëlle , PICHOT, Christian
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6813 , C08G81/02 , C12Q1/682
摘要： Kit for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The kit contains, in suitable containers, a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with said probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefore lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.
摘要翻译：用于检测感兴趣的核苷酸序列的试剂盒，其包含用示踪剂标记的至少一个核苷酸探针。试剂盒在合适的容器中含有基本上包含具有侧向取代基的线性骨架共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中至少一部分单元第一类型具有包含核苷酸单元的侧向取代基，这种侧向取代基不存在于其它类型的单元上。所述核苷酸单元中的每一个都相同，包含至少一个能够与所述感兴趣的序列杂交的核苷酸序列和能够与所述探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元，摩尔当量，每摩尔聚合物。这样的试剂能够获得信号放大，因此降低灵敏度阈值。特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。

2. 发明申请

WO9508000A3 REAGENT AND METHOD FOR THE DETECTION OF A NUCLEOTIDE SEQUENCE WITH SIGNAL AMPLIFICATION 审中-公开
标题翻译：用于检测具有信号放大的核苷酸序列的试剂和方法
公开(公告)号：WO9508000A3
公开(公告)日：1995-06-08
申请号：PCT/FR9401084
申请日：1994-09-15
申请人： BIO MERIEUX , MANDRAND BERNARD , CROS PHILIPPE , DELAIR THIERRY , CHARLES MARIE HELENE , EROUT MARIE NOELLE , PICHOT CHRISTIAN
发明人： MANDRAND BERNARD , CROS PHILIPPE , DELAIR THIERRY , CHARLES MARIE-HELENE , EROUT MARIE-NOELLE , PICHOT CHRISTIAN
IPC分类号： C08G81/02 , C12Q1/68
CPC分类号： C12Q1/6813 , C08G81/02 , C12Q1/682
摘要： Kit for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The kit contains, in suitable containers, a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with said probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefore lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.

3. 发明申请

WO1997034909A1 NUCLEIC ACID ISOLATION 审中-公开
标题翻译：核酸分离
公开(公告)号：WO1997034909A1
公开(公告)日：1997-09-25
申请号：PCT/FR1997000496
申请日：1997-03-20
申请人： BIO MERIEUX , CROS, Philippe , ELAISSARI, Abdelhamid , MABILAT, Claude , PICHOT, Christian , RODRIGUE, Marc , SANTORO, Lise
发明人： BIO MERIEUX
IPC分类号： C07H01/08
CPC分类号： C12N15/101 , C12N15/1006 , C12Q1/6806
摘要： A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) providing an adsorption reagent that includes a sol consisting of an aqueous continuous phase and a dispersed particulate substrate phase including a functionalised particulate polymer prepared by polymerising (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45 DEG C; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10 M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10 M is achieved.
摘要翻译：公开了一种从样品中分离水相核酸的方法，包括在颗粒基质上的核酸吸附步骤。该方法包括一种吸附试剂制备步骤（a），该步骤（a）提供一种吸附试剂，该吸附试剂包括由含水连续相组成的溶胶和分散的颗粒基质相，所述分散的颗粒底物相包括通过聚合制备的官能化颗粒聚合物（1）第一水溶性丙烯酰胺或丙烯酰胺衍生单体，（2）至少一种交联剂和（3）至少一种第二水溶性阳离子和功能性单体，所述聚合物具有25-45℃的预定的较低临界溶解温度（LCST）; 接触步骤（b），其中所述吸附剂与含有所述核酸的样品接触; 吸附步骤（c）其中，为了进行接触步骤（b），为反应介质选择至少一个参数，所述参数为不高于7的pH，不高于10 -2的离子强度 M，温度低于聚合物LCST; 分离步骤（d），其中分散相与连续相分离，任选地在已经观察到发生吸附之后; 和解吸步骤（e），其中通过增加离子强度直到达到高于10μM的离子强度，从核粒子脱离。

4. 发明申请

WO1996019729A1 HAPTENE ASSAY BY A COMPETITION-BASED METHOD 审中-公开
标题翻译：通过基于竞争的方法进行吸收测定
公开(公告)号：WO1996019729A1
公开(公告)日：1996-06-27
申请号：PCT/FR1995001709
申请日：1995-12-21
申请人： BIO MERIEUX , CROS, Philippe , BATTAIL, Nicole , PIGA, Nadia
发明人： BIO MERIEUX
IPC分类号： G01N33/53
CPC分类号： G01N33/532 , C12Q1/6804 , G01N33/54306 , G01N33/58 , G01N33/743
摘要： Method of assaying haptene in a sample, in which said sample is placed in contact with antibodies capable of recognizing haptene and with a predetermined quantity of a reagent capable of competing with said haptene-fixing antibodies. The reagent is a conjugate formed by a single-stranded fragment of nucleic acid with a compound capable of being recognized by said haptene-recognizing antibodies. The fixed or unfixed reactant is detected by antibodies capable of recognizing the nucleic acid fragment of said conjugate. Detection of said reactant by anti-fragment antibodies of said nucleic acid enhances, in particular, the sensitivity of the assay.
摘要翻译：在样品中测定半抗原的方法，其中所述样品与能够识别半抗原的抗体和预定量的能够与所述半抗原固定抗体竞争的试剂接触。试剂是由核酸的单链片段与能够被所述半抗原识别抗体识别的化合物形成的缀合物。通过能够识别所述缀合物的核酸片段的抗体检测固定或未固定的反应物。通过所述核酸的抗片段抗体检测所述反应物特别增强了测定的灵敏度。

5. 发明申请

WO1994029723A1 HAPTEN ASSAY DEVICE AND USE THEREOF 审中-公开
标题翻译：呼吸测试装置及其使用
公开(公告)号：WO1994029723A1
公开(公告)日：1994-12-22
申请号：PCT/FR1994000689
申请日：1994-06-10
申请人： BIO MERIEUX , CROS, Philippe , KURFURST, Robin , BATTAIL, Nicole , PIGA, Nadia
发明人： BIO MERIEUX
IPC分类号： G01N33/547
CPC分类号： C12Q1/6804 , G01N33/54306 , Y10S435/961 , Y10S435/969
摘要： A hapten or anti-hapten antibody assay device comprises a solid support and a reagent covalently linked to a ligand to form a conjugate immobilising said reagent on said solid support, the reagent being recognized by antibodies capable of recognizing the hapten. The invention is characterized in that the ligand is a nucleic acid fragment. It may be used in assaying a hapten in a liquid specimen using a competitive technique.
摘要翻译：半抗原或抗半抗原测定装置包括固体支持物和与配体共价连接以形成缀合物的试剂，所述缀合物固定所述固体支持物上的所述试剂，所述试剂由能够识别半抗原的抗体识别。本发明的特征在于配体是核酸片段。它可以用于使用竞争性技术测定液体样品中的半抗原。

6. 发明申请

WO1991019812A1 METHOD FOR DETECTING A NUCLEOTIDE SEQUENCE BY SANDWICH HYBRIDIZATION 审中-公开
标题翻译：通过三聚体杂化检测核苷酸序列的方法
公开(公告)号：WO1991019812A1
公开(公告)日：1991-12-26
申请号：PCT/FR1991000468
申请日：1991-06-11
申请人： BIO MERIEUX , CROS, Philippe , ALLIBERT, Patrice , MALLET, François , MABILAT, Claude , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6883 , C12Q1/6813 , C12Q1/6834 , C12Q1/6886 , C12Q1/689 , C12Q1/703 , C12Q1/708
摘要： A method for detecting a single-stranded nucleotide sequence in a sample which is known or thought to contain it, using sandwich hybridization comprising the step of incubating the sample with a capture probe which is passively fixed to a solid support and with a detector probe which is labelled with a non-radioactive marker. The capture and detector probes can hybridize with two non-overlapping regions respectively of the target nucleotide sequence being sought. The incubation step is followed by a washing step for removing reagents which have not been fixed by hybridization. The method is characterized in that the capture probe contains 9-30 nucleotides and is fixed to said solid support which is made of a material based on a hydrophobic compound. The method can be used particularly for diagnosing infectious or genetic diseases and for cell typing.

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