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1. 发明申请

WO2012008809A3 MICROORGANISM HAVING IMPROVED L-ORNITHINE PRODUCTIVITY, AND METHOD FOR PRODUCING L-ORNITHINE USING SAME 审中-公开
标题翻译：具有改进的L-ORNITHINE生产力的微生物和使用其生产L- ORN的方法
公开(公告)号：WO2012008809A3
公开(公告)日：2012-05-18
申请号：PCT/KR2011005251
申请日：2011-07-15
申请人： UNIV SANGJI INDUSTRY ACADEMIC COOPERATION FOUNDATION , CHO JAE YONG
发明人： CHO JAE YONG
IPC分类号： C12N1/21 , C12N15/54 , C12P13/10 , C12R1/15
CPC分类号： C12P13/10 , A23K20/142 , A23L33/175 , C12N9/1205
摘要： The present invention relates to an L-ornithine-producing microorganism, in which the gluconate kinase (GntK) activity is weakened relative to an intrinsic activity thereof, and to a method for producing L-ornithine from the L-ornithine-producing microorganism. According to the present invention, L-ornithine can be produced at a high yield rate and with high efficiency, and the thus-produced L-ornithine can be effectively used in the medical and pharmaceutical industry, the food industry, the feed industry, the chemical industry, etc.
摘要翻译：本发明涉及相对于其内在活性而使葡萄糖酸激酶（GntK）活性弱化的L-鸟氨酸生产微生物，以及L-赖氨酸生产微生物的L-鸟氨酸的制造方法。根据本发明，L-鸟氨酸可以以高产率和高效率生产，因此生产的L-鸟氨酸可以有效地用于医药工业，食品工业，饲料工业，化学工业等

2. 发明申请

WO2005023999A1 A METHOD FOR PRODUCING L-THREONINE 审中-公开
标题翻译：一种生产L-苏氨酸的方法
公开(公告)号：WO2005023999A1
公开(公告)日：2005-03-17
申请号：PCT/KR2003/001842
申请日：2003-09-06
申请人： CJ CORPORATION , CHO, Jae-Yong , LEE, Byoung-Choon , KIM, Dae-Cheol , LEE, Jin-Ho , PARK, Young-Hoon
发明人： CHO, Jae-Yong , LEE, Byoung-Choon , KIM, Dae-Cheol , LEE, Jin-Ho , PARK, Young-Hoon
IPC分类号： C12N1/20
CPC分类号： C12P13/08
摘要： An L-threonine-producing Escherichia coli strain and a method for producing the same are provided. The Escherichia coli strain contains chromosomal DNA with inactivated metJ gene. Therefore, expression repression of threonine biosynthesis genes by a metJ gene product is prevented, thereby producing a high concentration of threonine. Further, a high concentration of L-threonine can be produced in high yield using the method.
摘要翻译：提供了产生L-苏氨酸的大肠杆菌菌株及其制备方法。大肠杆菌菌株含有具有灭活的metJ基因的染色体DNA。因此，通过metJ基因产物抑制苏氨酸生物合成基因的表达抑制，从而产生高浓度的苏氨酸。此外，可以使用该方法以高产率制备高浓度的L-苏氨酸。

3. 发明申请

WO2012161522A2 MICROORGANISM FOUND IN THE CORYNEBACTERIUM GENUS HAVING ENHANCED L-ORNITHINE PRODUCTION AND METHOD FOR PREPARING L-ORNITHINE USING SAME 审中-公开
标题翻译：在具有增强的L-选择素生产的CORYNBACTERIUM基因中发现的微生物和使用它们制备L-ORNITH的方法
公开(公告)号：WO2012161522A2
公开(公告)日：2012-11-29
申请号：PCT/KR2012004089
申请日：2012-05-23
申请人： GANGWON TECHNOLOGY HOLDINGS CO LTD , CHO JAE YONG , KO YOUNG WOONG
发明人： CHO JAE YONG , KO YOUNG WOONG
IPC分类号： C12N1/21 , C12N15/77 , C12P13/10 , C12R1/15
CPC分类号： C12N9/0006 , C12P13/10 , C12Y101/9901
摘要： The present invention relates to a microorganism found in the corynebacterium genus having enhanced L-ornithine production, and to a method for preparing L-ornithine using same. More particularly, the present invention relates to a microorganism found in the corynebacterium genus in which intrinsic glucose dehydrogenase (GDH) activity is reduced or becomes inert, thus enhancing L-ornithine production. The present invention also relates to a method for preparing said microorganism, and to a method for preparing L-ornithine, comprising the steps of: culturing said microorganism using glucose as a carbon source; and recovering L-ornithine from said culture. Using the microorganism found in the corynebacterium genus of the present invention in which intrinsic glucose dehydrogenase enzyme activity is reduced, not only is a pentose phosphate pathway activated but also the specific activities of G6PDH and 6PGD, which are enzymes related to the pentose phosphate pathway, are increased to improve NADPH production yields, thus increasing L-ornithine production yields using said NADPH. Therefore, the microorganism of the present invention may be widely utilized in the efficient and economical production of L-ornithine.
摘要翻译：本发明涉及具有增强的L-鸟氨酸生产的棒状杆菌属中发现的微生物以及使用其制备L-鸟氨酸的方法。更具体地说，本发明涉及其中内源性葡萄糖脱氢酶（GDH）活性降低或变成惰性的棒状杆菌属中发现的微生物，从而增强L-鸟氨酸生产。本发明还涉及一种制备所述微生物的方法和L-鸟氨酸的制备方法，包括以下步骤：使用葡萄糖作为碳源培养所述微生物; 并从所述文化中回收L-鸟氨酸。使用其中内在葡萄糖脱氢酶活性降低的本发明的棒状杆菌属中发现的微生物不仅活化戊糖磷酸途径，而且与作为与戊糖磷酸途径相关的酶的G6PDH和6PGD的比活性，增加NADPH生产产率，从而增加使用所述NADPH的L-鸟氨酸产量。因此，本发明的微生物可以广泛地用于L-鸟氨酸的有效和经济的生产。

4. 发明申请

WO2011083933A3 MUTANT STRAIN FOR PRODUCING L-ORNITHINE OR L-ARGININE, AND METHOD FOR PRODUCING SAME 审中-公开
标题翻译：用于生产L-鸟氨酸或L-鸟氨酸的突变株及其制备方法
公开(公告)号：WO2011083933A3
公开(公告)日：2011-11-10
申请号：PCT/KR2010009521
申请日：2010-12-29
申请人： CJ CHEILJEDANG CORP , CHO JIN-MAN , KIM HYE-WON , LEE JI-HYE , CHO JAE-YONG
发明人： CHO JIN-MAN , KIM HYE-WON , LEE JI-HYE , CHO JAE-YONG
IPC分类号： C12N9/00 , C12N15/52 , C12P13/10
CPC分类号： C12P13/10 , C12N9/1029 , C12N9/80 , C12Y203/01001 , C12Y305/01016
摘要： The present invention relates to a polynucleotide that is active to an acetyl glutamate synthase and acetyl ornithinase which are associated with ornithine or arginine biosynthesis from Corynebacterium glutamicum. The present invention also relates to a polypeptide encoded by said polynucleotide, a recombinant vector comprising said polynucleotide, to a transformant obtained by introducing said recombinant vector to a host microorganism for producing L-ornithine or L-arginine, and transforming the recombinant vector, and to a method for producing L-ornithine or L-arginine by culturing said transformant. The activity of the transformant of the present invention to an acetyl glutamate synthase and acetyl ornithinase is increased as compared to an intrinsic activity, and thus L-ornithine or L-arginine can be produced, at a high yield rate, from the transformant of the present invention.
摘要翻译：本发明涉及对来自谷氨酸棒状杆菌的鸟氨酸或精氨酸生物合成有关的乙酰谷氨酸合酶和乙酰鸟氨酸酶有活性的多核苷酸。本发明还涉及由所述多核苷酸编码的多肽，包含所述多核苷酸的重组载体，通过将所述重组载体导入用于产生L-鸟氨酸或L-精氨酸的宿主微生物获得的转化体，以及转化该重组载体，以及涉及通过培养所述转化体来生产L-鸟氨酸或L-精氨酸的方法。与固有活性相比，本发明的转化体对乙酰谷氨酸合成酶和乙酰鸟氨酸酶的活性增加，因此可以以高收率从转化体中产生L-鸟氨酸或L-精氨酸本发明。

5. 发明申请

WO2004033671A1 THE MICROORGANISM OF WHICH THE FADR GENE WAS KNOCKED OUT ON THE CHROMOSOME AND A PROCESS FOR PRODUCING L-THREONINE BY FERMENTATION WITH THE SAME MUTANT 审中-公开
标题翻译： FADR基因在染色体上被捕获的微生物和通过与相同突变体发酵生产L-酪氨酸的方法
公开(公告)号：WO2004033671A1
公开(公告)日：2004-04-22
申请号：PCT/KR2003/000906
申请日：2003-05-07
申请人： CJ CORP. , PARK, Young-Hoon , LEE, Jin-Ho , LEE, Byoung-Choon , KIM, Dae-Cheol , CHO, Jae-Yong
发明人： PARK, Young-Hoon , LEE, Jin-Ho , LEE, Byoung-Choon , KIM, Dae-Cheol , CHO, Jae-Yong
IPC分类号： C12N1/21
CPC分类号： C12R1/19 , C12P13/08
摘要： The present invention relates to a L-threonine-producing microorganism characterized in that the fad R gene present on the chromosome of the microorganism has been knocked out and a method for producing L-threonine using said mutated mircroorganism. The mutated microorganism of the present invention increases the production of L-threonine.
摘要翻译：本发明涉及产生L-苏氨酸的微生物，其特征在于，存在于微生物染色体上的fadR基因被敲除，使用所述突变的微生物产生L-苏氨酸的方法。本发明的突变微生物增加了L-苏氨酸的产生。

6. 发明申请

WO2003080843A1 METHOD FOR L-THREONINE PRODUCTION 审中-公开
标题翻译： L-螺旋藻生产方法
公开(公告)号：WO2003080843A1
公开(公告)日：2003-10-02
申请号：PCT/KR2002/000920
申请日：2002-05-16
申请人： CHEIL JEDANG CORPORATION , PARK, Jae-yong , LEE, Byoung-choon , KIM, Dae-cheol , LEE, Jin-ho , CHO, Jae-yong , PARK, Young-hoon
发明人： PARK, Jae-yong , LEE, Byoung-choon , KIM, Dae-cheol , LEE, Jin-ho , CHO, Jae-yong , PARK, Young-hoon
IPC分类号： C12N15/70
CPC分类号： C12N9/88 , C12P13/08
摘要： A method for producing L-threonine using a microorganism is provided. In the method, the threonine dehydratase (tdc) gene existing in the genomic DNA of the microorganism is partially deactivated using a recombination technique. For a microorganism strain with enhanced activity of threonine operon-containing enzymes and the phosphoenolpyruvate carboxylase (ppc) gene, the tdc gene engaged in one of the four threonine metabolic pathways is specifically deactivated, thereby markedly increasing the yield of L-threonine.
摘要翻译：提供了使用微生物生产L-苏氨酸的方法。在该方法中，存在于微生物的基因组DNA中的苏氨酸脱水酶（tdc）基因使用重组技术部分失活。对于具有增强的苏氨酸操纵子酶和磷酸烯醇丙酮酸羧化酶（ppc）基因的活性的微生物菌株，特异性地失活了四种苏氨酸代谢途径之一的tdc基因，从而显着提高L-苏氨酸的产量。

7. 发明申请

WO2012008810A3 MICROORGANISM WITH ENHANCED L-LYSINE PRODUCTIVITY AND METHOD FOR PRODUCING L-LYSINE USING THE SAME 审中-公开
标题翻译：具有增强的L-赖氨酸生产力的微生物及使用该生产L-赖氨酸的方法
公开(公告)号：WO2012008810A3
公开(公告)日：2012-05-24
申请号：PCT/KR2011005252
申请日：2011-07-15
申请人： CJ CHEILJEDANG CORP , LIM SANG JO , MOON JUN OK , KIM HYUNG JOON , JANG JAE WOO , CHO JAE YONG
发明人： LIM SANG JO , MOON JUN OK , KIM HYUNG JOON , JANG JAE WOO , CHO JAE YONG
IPC分类号： C12N1/21 , C12N15/54 , C12P13/08 , C12R1/15
CPC分类号： C12P13/08 , C12N9/1205
摘要： Disclosed is an L-lysine-producing microorganism having gluconate kinase activity weakened in comparison to the endogenous activity thereof, and methods provided for preparing the microorganism and for producing L-lysine using the same.
摘要翻译：公开了具有葡糖酸激酶活性的产生L-赖氨酸的微生物与其内源性活性相比减弱，并提供了用于制备该微生物和使用该微生物生产L-赖氨酸的方法。

8. 发明申请

WO2012008810A2 MICROORGANISM WITH ENHANCED L-LYSINE PRODUCTIVITY AND METHOD FOR PRODUCING L-LYSINE USING THE SAME 审中-公开
标题翻译：具有增强的L-赖氨酸生产力的微生物和使用其生产L-赖氨酸的方法
公开(公告)号：WO2012008810A2
公开(公告)日：2012-01-19
申请号：PCT/KR2011/005252
申请日：2011-07-15
申请人： CJ CHEILJEDANG CORPORATION , LIM, Sang Jo , MOON, Jun Ok , KIM, Hyung Joon , JANG, Jae Woo , CHO, Jae Yong
发明人： LIM, Sang Jo , MOON, Jun Ok , KIM, Hyung Joon , JANG, Jae Woo , CHO, Jae Yong
IPC分类号： C12N1/21 , C12N15/54 , C12P13/08 , C12R1/15
CPC分类号： C12P13/08 , C12N9/1205
摘要： Disclosed is an L-lysine-producing microorganism having gluconate kinase activity weakened in comparison to the endogenous activity thereof, and methods provided for preparing the microorganism and for producing L-lysine using the same.
摘要翻译：公开了具有比其内源性活性弱的葡萄糖酸激酶活性的L-赖氨酸生产微生物，以及提供用于制备微生物并使用其生产L-赖氨酸的方法。

9. 发明申请

WO2011078443A1 DETECTING MARKER FOR GASTRIC CANCER AND DETECTING METHOD USING THE SAME MARKER 审中-公开
标题翻译：检测胃癌标记物和使用相同标记的检测方法
公开(公告)号：WO2011078443A1
公开(公告)日：2011-06-30
申请号：PCT/KR2010/002416
申请日：2010-04-19
申请人： CHO, Jae-Yong , LIM, Jae Yun
发明人： CHO, Jae-Yong , LIM, Jae Yun
IPC分类号： C12Q1/68 , C12N15/11 , G01N33/574
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/118 , C12Q2600/136 , C12Q2600/158 , G01N33/57446
摘要： A composition for detecting gastric cancer including an agent for detecting the expression level of mRNA of one or more gene(s) selected from a group consisting of TXN and TXNIP or protein thereof is disclosed. Also, a detection kit, a detecting method and a screening method are disclosed. The composition allows effective detection of the incidence of gastric cancer and may be widely applicable, for example, as an agent for treating gastric cancer.
摘要翻译：公开了一种用于检测胃癌的组合物，其包括用于检测选自由TXN和TXNIP或其蛋白质组成的组中的一种或多种基因的mRNA的表达水平的试剂。另外，公开了一种检测试剂盒，检测方法和筛选方法。该组合物可有效检测胃癌的发生率，并且可广泛应用于例如胃癌治疗药物。

10. 发明申请

WO2004087895A1 tdcBC/pckA GENE-INACTIVATED MICROORGANISM AND METHOD OF PRODUCING L-THREONINE USING THE SAME 审中-公开
标题翻译： tdcBC / pckA基因灭活微生物及使用其生产L-苏氨酸的方法
公开(公告)号：WO2004087895A1
公开(公告)日：2004-10-14
申请号：PCT/KR2004/000778
申请日：2004-04-02
申请人： CJ CORP. , PARK, Young Hoon , LEE, Byoung Choon , KIM, Dae Cheol , LEE, Jin Ho , CHO, Jae Yong
发明人： PARK, Young Hoon , LEE, Byoung Choon , KIM, Dae Cheol , LEE, Jin Ho , CHO, Jae Yong
IPC分类号： C12N1/21
CPC分类号： C12P13/08 , C12N9/88
摘要： Disclosed is a microorganism including both tdcBC and pckA genes inactivated on chromosome, which has remarkably improved productivity of L-thionine due to the inactivation of the two genes. Also, the present invention discloses a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.
摘要翻译：公开了一种微生物，其包括在染色体上灭活的tdcBC和pckA基因，由于两种基因的失活，其显着提高了L-硫素的生产力。此外，本发明公开了使用该微生物生产L-苏氨酸的方法。通过重组技术将抗生素抗性基因并入包含失活的L-苏氨酸降解相关操纵子基因tdcBC的细菌菌株的染色体上的pckA基因中来制备微生物。该微生物具有防止由于tdcBC操纵子基因失活引起的L-苏氨酸的降解和细胞内流入的作用，并且包括用于L-苏氨酸生物合成的更多活化途径。因此，即使在高浓度葡萄糖的存在下，微生物也能够以高水平和高产率生产L-苏氨酸，因此可用于批量生产L-苏氨酸。

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