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1. 发明申请

WO2004087895A1 tdcBC/pckA GENE-INACTIVATED MICROORGANISM AND METHOD OF PRODUCING L-THREONINE USING THE SAME 审中-公开
标题翻译： tdcBC / pckA基因灭活微生物及使用其生产L-苏氨酸的方法
公开(公告)号：WO2004087895A1
公开(公告)日：2004-10-14
申请号：PCT/KR2004/000778
申请日：2004-04-02
申请人： CJ CORP. , PARK, Young Hoon , LEE, Byoung Choon , KIM, Dae Cheol , LEE, Jin Ho , CHO, Jae Yong
发明人： PARK, Young Hoon , LEE, Byoung Choon , KIM, Dae Cheol , LEE, Jin Ho , CHO, Jae Yong
IPC分类号： C12N1/21
CPC分类号： C12P13/08 , C12N9/88
摘要： Disclosed is a microorganism including both tdcBC and pckA genes inactivated on chromosome, which has remarkably improved productivity of L-thionine due to the inactivation of the two genes. Also, the present invention discloses a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.
摘要翻译：公开了一种微生物，其包括在染色体上灭活的tdcBC和pckA基因，由于两种基因的失活，其显着提高了L-硫素的生产力。此外，本发明公开了使用该微生物生产L-苏氨酸的方法。通过重组技术将抗生素抗性基因并入包含失活的L-苏氨酸降解相关操纵子基因tdcBC的细菌菌株的染色体上的pckA基因中来制备微生物。该微生物具有防止由于tdcBC操纵子基因失活引起的L-苏氨酸的降解和细胞内流入的作用，并且包括用于L-苏氨酸生物合成的更多活化途径。因此，即使在高浓度葡萄糖的存在下，微生物也能够以高水平和高产率生产L-苏氨酸，因此可用于批量生产L-苏氨酸。

2. 发明申请

WO2012134253A3 CORYNEBACTERIUM SP. TRANSFORMED WITH A FRUCTOKINASE GENE DERIVED FROM ESCHERICHIA SP. AND PROCESS FOR PREPARING L-AMINO ACID USING THE SAME 审中-公开
标题翻译：红霉素用来自ESCHERICHIA SP的FRUCTOKINASE基因转化。和使用该方法制备L-氨基酸的方法和方法
公开(公告)号：WO2012134253A3
公开(公告)日：2012-12-27
申请号：PCT/KR2012002474
申请日：2012-04-02
申请人： CJ CHEILJEDANG CORP , BAE HYUN WON , KIM HYUNG JOON , MOON JUN OK , JANG JAE WOO , KIM JONG CHUL , KIM TAE HAN , SUNG JIN SUCK , LEE KYUNG HAN , KIM DAE CHEOL , KIM HYO JIN , BAE HYUN AE , LIM SANG JO
发明人： BAE HYUN WON , KIM HYUNG JOON , MOON JUN OK , JANG JAE WOO , KIM JONG CHUL , KIM TAE HAN , SUNG JIN SUCK , LEE KYUNG HAN , KIM DAE CHEOL , KIM HYO JIN , BAE HYUN AE , LIM SANG JO
IPC分类号： C12N1/21 , C12N15/31 , C12N15/77 , C12P13/04
CPC分类号： C12P13/14 , C12N9/1205 , C12P13/04 , C12P13/08 , C12Y207/01004
摘要： The present invention relates to Corynebacterium sp. that is transformed with an Escherichia sp.-derived fructokinase gene to express fructokinase showing a sufficient activity of converting fructose into fructose-6-phosphate, thereby preventing unnecessary energy consumption, and a method for producing L-amino acids using the strain. The transformed Corynebacterium sp. of the present invention is able to express fructokinase from the Escherichia-derived fructokinase gene to prevent unnecessary energy consumption during fructose metabolism, leading to more cost-effective production of L-amino acids. Therefore, it can be widely used for the effective production of L-amino acids.
摘要翻译：本发明涉及棒状杆菌属用Escherichia sp.衍生的果糖激酶基因转化以表达果糖激酶，其显示出将果糖转化为果糖-6-磷酸的足够活性，从而防止不必要的能量消耗，以及使用该菌株产生L-氨基酸的方法。转化的棒状杆菌属本发明能够从来自大肠杆菌的果糖激酶基因中表达果糖激酶，以防止在果糖代谢期间不必要的能量消耗，导致L-氨基酸的更具成本效益的生产。因此，可广泛用于L-氨基酸的有效生产。

3. 发明申请

WO2005071061A1 A METHOD FOR PRODUCING LACTIC ACID WITH HIGH CONCENTRATION AND HIGH YIELD USING LACTIC ACID BACTERIA 审中-公开
标题翻译：一种使用酸性细菌生产高浓度和高产量的酸的方法
公开(公告)号：WO2005071061A1
公开(公告)日：2005-08-04
申请号：PCT/KR2004/002991
申请日：2004-11-18
申请人： CJ CORP. , PARK, Young-Hoon , CHO, Kwang-Myung , KIM, Hye-Won , KIM, Dae-Cheol
发明人： PARK, Young-Hoon , CHO, Kwang-Myung , KIM, Hye-Won , KIM, Dae-Cheol
IPC分类号： C12N1/20
CPC分类号： C12N1/20 , C12P7/56 , C12R1/225
摘要： The present invention relates to a method for producing lactic acid with high concentration and high yield using Lactobacillus paracasei CJLA0310 KCCM-10542 that is separated and identified from Kimchi. Lactic acid is a very important organic acid with a wide range of applications including food additive such as food preservative, condiment or acidifier, and industrial fields such as cosmetics, chemistry, metals, electronics, fabrics, dyeing textiles, and pharmaceutical industries. Particularly, lactic acid is an essential ingredient of polylactic acid, one of biodegradable plastics to replace recalcitrant non-biodegradable plastics which are main causes of environmental contamination.
摘要翻译：本发明涉及使用从泡菜分离鉴定的副干酪乳杆菌CJLA0310 KCCM-10542来生产高浓度和高产量的乳酸的方法。乳酸是一种非常重要的有机酸，适用范围广泛，包括食品添加剂如食品防腐剂，调味品或酸化剂，以及化妆品，化学，金属，电子，织物，染色纺织品和制药工业等工业领域。特别地，乳酸是聚乳酸的重要成分，聚乳酸是能够替代作为环境污染的主要原因的顽固性不可生物降解塑料的生物降解塑料之一。

4. 发明申请

WO2004033671A1 THE MICROORGANISM OF WHICH THE FADR GENE WAS KNOCKED OUT ON THE CHROMOSOME AND A PROCESS FOR PRODUCING L-THREONINE BY FERMENTATION WITH THE SAME MUTANT 审中-公开
标题翻译： FADR基因在染色体上被捕获的微生物和通过与相同突变体发酵生产L-酪氨酸的方法
公开(公告)号：WO2004033671A1
公开(公告)日：2004-04-22
申请号：PCT/KR2003/000906
申请日：2003-05-07
申请人： CJ CORP. , PARK, Young-Hoon , LEE, Jin-Ho , LEE, Byoung-Choon , KIM, Dae-Cheol , CHO, Jae-Yong
发明人： PARK, Young-Hoon , LEE, Jin-Ho , LEE, Byoung-Choon , KIM, Dae-Cheol , CHO, Jae-Yong
IPC分类号： C12N1/21
CPC分类号： C12R1/19 , C12P13/08
摘要： The present invention relates to a L-threonine-producing microorganism characterized in that the fad R gene present on the chromosome of the microorganism has been knocked out and a method for producing L-threonine using said mutated mircroorganism. The mutated microorganism of the present invention increases the production of L-threonine.
摘要翻译：本发明涉及产生L-苏氨酸的微生物，其特征在于，存在于微生物染色体上的fadR基因被敲除，使用所述突变的微生物产生L-苏氨酸的方法。本发明的突变微生物增加了L-苏氨酸的产生。

5. 发明申请

WO2005023999A1 A METHOD FOR PRODUCING L-THREONINE 审中-公开
标题翻译：一种生产L-苏氨酸的方法
公开(公告)号：WO2005023999A1
公开(公告)日：2005-03-17
申请号：PCT/KR2003/001842
申请日：2003-09-06
申请人： CJ CORPORATION , CHO, Jae-Yong , LEE, Byoung-Choon , KIM, Dae-Cheol , LEE, Jin-Ho , PARK, Young-Hoon
发明人： CHO, Jae-Yong , LEE, Byoung-Choon , KIM, Dae-Cheol , LEE, Jin-Ho , PARK, Young-Hoon
IPC分类号： C12N1/20
CPC分类号： C12P13/08
摘要： An L-threonine-producing Escherichia coli strain and a method for producing the same are provided. The Escherichia coli strain contains chromosomal DNA with inactivated metJ gene. Therefore, expression repression of threonine biosynthesis genes by a metJ gene product is prevented, thereby producing a high concentration of threonine. Further, a high concentration of L-threonine can be produced in high yield using the method.
摘要翻译：提供了产生L-苏氨酸的大肠杆菌菌株及其制备方法。大肠杆菌菌株含有具有灭活的metJ基因的染色体DNA。因此，通过metJ基因产物抑制苏氨酸生物合成基因的表达抑制，从而产生高浓度的苏氨酸。此外，可以使用该方法以高产率制备高浓度的L-苏氨酸。

6. 发明申请

WO2012134253A2 CORYNEBACTERIUM SP. TRANSFORMED WITH A FRUCTOKINASE GENE DERIVED FROM ESCHERICHIA SP. AND PROCESS FOR PREPARING L-AMINO ACID USING THE SAME 审中-公开
标题翻译： CORYNEBACTERIUM SP。用来自ESCHERICHIA SP的FRUTOKINASE基因转化。以及使用该方法制备L-氨基酸的方法
公开(公告)号：WO2012134253A2
公开(公告)日：2012-10-04
申请号：PCT/KR2012/002474
申请日：2012-04-02
申请人： CJ CHEILJEDANG CORPORATION , BAE, Hyun Won , KIM, Hyung Joon , MOON, Jun Ok , JANG, Jae Woo , KIM, Jong Chul , KIM, Tae Han , SUNG, Jin Suck , LEE, Kyung Han , KIM, Dae Cheol , KIM, Hyo Jin , BAE, Hyun Ae , LIM, Sang Jo
发明人： BAE, Hyun Won , KIM, Hyung Joon , MOON, Jun Ok , JANG, Jae Woo , KIM, Jong Chul , KIM, Tae Han , SUNG, Jin Suck , LEE, Kyung Han , KIM, Dae Cheol , KIM, Hyo Jin , BAE, Hyun Ae , LIM, Sang Jo
IPC分类号： C12N1/21 , C12N15/31 , C12N15/77 , C12P13/04
CPC分类号： C12P13/14 , C12N9/1205 , C12P13/04 , C12P13/08 , C12Y207/01004
摘要： The present invention relates to Corynebacterium sp. that is transformed with an Escherichia sp.-derived fructokinase gene to express fructokinase showing a sufficient activity of converting fructose into fructose-6-phosphate, thereby preventing unnecessary energy consumption, and a method for producing L-amino acids using the strain. The transformed Corynebacterium sp. of the present invention is able to express fructokinase from the Escherichia -derived fructokinase gene to prevent unnecessary energy consumption during fructose metabolism, leading to more cost-effective production of L-amino acids. Therefore, it can be widely used for the effective production of L-amino acids.
摘要翻译：本发明涉及棒状杆菌属（Corynebacterium sp。）。它被来源于大肠杆菌的果糖激酶基因转化以表达果糖激酶，该果糖激酶显示出将果糖转化成果糖-6-磷酸的充分活性，由此防止不必要的能量消耗，以及产生L-氨基酸的方法使用该菌株的酸。转化的棒状杆菌属物种（Corynebacterium sp。）能够从来源于埃希氏菌属的果糖激酶基因表达果糖激酶，以防止在果糖代谢过程中不必要的能量消耗，从而导致L-氨基酸的生产成本更低。因此，它可以广泛用于有效生产L-氨基酸。

7. 发明申请

WO2003080843A1 METHOD FOR L-THREONINE PRODUCTION 审中-公开
标题翻译： L-螺旋藻生产方法
公开(公告)号：WO2003080843A1
公开(公告)日：2003-10-02
申请号：PCT/KR2002/000920
申请日：2002-05-16
申请人： CHEIL JEDANG CORPORATION , PARK, Jae-yong , LEE, Byoung-choon , KIM, Dae-cheol , LEE, Jin-ho , CHO, Jae-yong , PARK, Young-hoon
发明人： PARK, Jae-yong , LEE, Byoung-choon , KIM, Dae-cheol , LEE, Jin-ho , CHO, Jae-yong , PARK, Young-hoon
IPC分类号： C12N15/70
CPC分类号： C12N9/88 , C12P13/08
摘要： A method for producing L-threonine using a microorganism is provided. In the method, the threonine dehydratase (tdc) gene existing in the genomic DNA of the microorganism is partially deactivated using a recombination technique. For a microorganism strain with enhanced activity of threonine operon-containing enzymes and the phosphoenolpyruvate carboxylase (ppc) gene, the tdc gene engaged in one of the four threonine metabolic pathways is specifically deactivated, thereby markedly increasing the yield of L-threonine.
摘要翻译：提供了使用微生物生产L-苏氨酸的方法。在该方法中，存在于微生物的基因组DNA中的苏氨酸脱水酶（tdc）基因使用重组技术部分失活。对于具有增强的苏氨酸操纵子酶和磷酸烯醇丙酮酸羧化酶（ppc）基因的活性的微生物菌株，特异性地失活了四种苏氨酸代谢途径之一的tdc基因，从而显着提高L-苏氨酸的产量。

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