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    • 2. 发明申请
    • SUBSTRATES, PREPARATION AND USE
    • 基材,制备和使用
    • WO2002078835A2
    • 2002-10-10
    • PCT/EP2002/003102
    • 2002-03-20
    • UCB, S.A.LINDEKENS, LucTIELEMANS, MichelCAPPELLE, StevenREMACLE, JoseDE BECKER, Benoit
    • LINDEKENS, LucTIELEMANS, MichelCAPPELLE, StevenREMACLE, JoseDE BECKER, Benoit
    • B01J19/00
    • G01N33/54366B01J19/0046B01J2219/00527B01J2219/00585B01J2219/00596B01J2219/00605B01J2219/00608B01J2219/0061B01J2219/00626B01J2219/00637B01J2219/00659B01J2219/00707B01J2219/0072B01J2219/00722C40B40/06G01N33/54386G01N33/54393Y10T428/31504
    • There is disclosed process for preparing a substrate 1. Process for preparing a substrate having thereon sites reactive with the reactive groups of a molecular probe, the process comprising the steps of applying to the substrate surface a material comprising one or more reactive sites having an activated ethylenically unsaturated double bond of the formula I: Wherein, if n= 0, m= 1, X 3 is carbon and X 1 is nitrogen, the bond between them being triple, and X 4 does not exist; if n= 1, m= 1, X 3 is carbon and X 1 is oxygen or sulphur, the bond between them being double, and X 2 is oxygen or sulphur or nitrogen substituted by an organo substituent; if n= 1, m= 1 or 2, X 3 is sulphur and X 1 is oxygen, the bond between them being double, and X 2 is nitrogen substituted by an organo substituent; R 1 , R 2 , each independently represent hydrogen, hydroxyl or organo group, R 3 and R 4 each independently represent hydrogen or organo group, eventually containing a silicon atom R 1 and R 2 being not linked to R 4 . and, in a further step, of covalently binding the reactive group of the molecular probe to the activated ethylenically unsaturated double bond of the reactive sites having formula I. Preferred activated unsaturated moiet(ies) comprises unsaturated ester moiet(ies) such as (meth)acrylate moiet(ies). Preferred probes comprise amino groups thereon. Preferred strong links are covalent bonds. Preferred linking reactions are Michael addition reactions. The substrate having probes deposed thereon may be used to interact with a target. Other aspects of the invention relate to substrates and multi-reactive systems (such as microarrays) so prepared; uses thereof; and products and information obtained therefrom.
    • 公开了制备基材的方法。1.制备在其上具有与分子探针的反应性基团反应的位点的基材的方法,所述方法包括以下步骤:将包含 具有式I活化的烯属不饱和双键的一个或多个反应性位点:其中,如果n = 0,m = 1,X 3是碳并且X 1是 氮,它们之间的键是三重的,并且X 4不存在; 如果n = 1,m = 1,X 3是碳并且X 1是氧或硫,它们之间的键是双键,并且X 2 = 是被有机取代基取代的氧或硫或氮; 如果n = 1,m = 1或2,X 3是硫而X 1是氧,它们之间的键是双键,并且X 2 = 是被有机取代基取代的氮; R 1,R 2各自独立地表示氢,羟基或有机基团,R 3和R 4分别为 独立地表示氢或有机基团,最终含有硅原子R 1和R 2不连接到R 4上。 并且在进一步的步骤中将分子探针的反应性基团共价结合到具有式I的反应活性部位的活化的烯键式不饱和双键上。优选的活化不饱和醇包括不饱和酯(例如(甲基)丙烯酸酯 )丙烯酸酯(酯)。 优选的探针在其上包含氨基。 优选的强链接是共价键。 优选的连接反应是迈克尔加成反应。 具有置于其上的探针的衬底可以用于与目标相互作用。 本发明的其他方面涉及如此制备的底物和多反应系统(如微阵列) 其用途; 以及从中获得的产品和信息。
    • 4. 发明申请
    • SUBSTRATES, PREPARATION AND USE
    • 基材,制备和使用
    • WO02078835A3
    • 2002-11-28
    • PCT/EP0203102
    • 2002-03-20
    • UCB SALINDEKENS LUCTIELEMANS MICHELCAPPELLE STEVENREMACLE JOSEDE BECKER BENOIT
    • LINDEKENS LUCTIELEMANS MICHELCAPPELLE STEVENREMACLE JOSEDE BECKER BENOIT
    • C12N15/09B01J19/00C08F291/00C08G85/00C40B40/06G01N33/543B01L3/00C08F20/02C12Q1/68
    • G01N33/54366B01J19/0046B01J2219/00527B01J2219/00585B01J2219/00596B01J2219/00605B01J2219/00608B01J2219/0061B01J2219/00626B01J2219/00637B01J2219/00659B01J2219/00707B01J2219/0072B01J2219/00722C40B40/06G01N33/54386G01N33/54393Y10T428/31504
    • There is disclosed process for preparing a substrate 1. Process for preparing a substrate having thereon sites reactive with the reactive groups of a molecular probe, the process comprising the steps of applying to the substrate surface a material comprising one or more reactive sites having an activated ethylenically unsaturated double bond of the formula I: Wherein, if n= 0, m= 1, X is carbon and X is nitrogen, the bond between them being triple, and X does not exist; if n= 1, m= 1, X is carbon and X is oxygen or sulphur, the bond between them being double, and X is oxygen or sulphur or nitrogen substituted by an organo substituent; if n= 1, m= 1 or 2, X is sulphur and X is oxygen, the bond between them being double, and X is nitrogen substituted by an organo substituent; R1, R2, each independently represent hydrogen, hydroxyl or organo group, R3 and R4 each independently represent hydrogen or organo group, eventually containing a silicon atom R1 and R2 being not linked to R4. and, in a further step, of covalently binding the reactive group of the molecular probe to the activated ethylenically unsaturated double bond of the reactive sites having formula I. Preferred activated unsaturated moiet(ies) comprises unsaturated ester moiet(ies) such as (meth)acrylate moiet(ies). Preferred probes comprise amino groups thereon. Preferred strong links are covalent bonds. Preferred linking reactions are Michael addition reactions. The substrate having probes deposed thereon may be used to interact with a target. Other aspects of the invention relate to substrates and multi-reactive systems (such as microarrays) so prepared; uses thereof; and products and information obtained therefrom.
    • 公开了制备基底的方法1.制备其上具有与分子探针的反应性基团反应的位点的基底的方法,该方法包括以下步骤:向基底表面施加包含一个或多个具有活化的 式I的烯属不饱和双键:其中,如果n = 0,m = 1,X 3为碳,X 1为氮,它们之间的键为三价,X 4不存在; 如果n = 1,m = 1,X 3为碳,X 1为氧或硫,它们之间的键为双键,X 2为氧或被有机取代基取代的硫或氮; 如果n = 1,m = 1或2,X 3为硫,X 1为氧,它们之间的键为双,X 2为被有机取代基取代的氮; R 1,R 2各自独立地表示氢,羟基或有机基团,R 3和R 4各自独立地表示氢或有机基团,最终含有硅原子,R 1和R 2不与R 4连接。 并且在另一步骤中,将分子探针的反应性基团共价结合到具有式I的反应性位点的活化的烯属不饱和双键上。优选的活化的不饱和疏水物包括不饱和酯类,例如(甲基 )丙烯酸酯。 优选的探针包括其上的氨基。 优先的联系是共价债券。 优选的连接反应是迈克尔加成反应。 可以使用其上放置的探针的基底与靶相互作用。 本发明的其它方面涉及如此制备的底物和多反应体系(如微阵列); 用途; 以及从中获得的产品和信息。