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1. 发明申请

WO2002078835A2 SUBSTRATES, PREPARATION AND USE 审中-公开
标题翻译：基材，制备和使用
公开(公告)号：WO2002078835A2
公开(公告)日：2002-10-10
申请号：PCT/EP2002/003102
申请日：2002-03-20
申请人： UCB, S.A. , LINDEKENS, Luc , TIELEMANS, Michel , CAPPELLE, Steven , REMACLE, Jose , DE BECKER, Benoit
发明人： LINDEKENS, Luc , TIELEMANS, Michel , CAPPELLE, Steven , REMACLE, Jose , DE BECKER, Benoit
IPC分类号： B01J19/00
CPC分类号： G01N33/54366 , B01J19/0046 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00707 , B01J2219/0072 , B01J2219/00722 , C40B40/06 , G01N33/54386 , G01N33/54393 , Y10T428/31504
摘要： There is disclosed process for preparing a substrate 1. Process for preparing a substrate having thereon sites reactive with the reactive groups of a molecular probe, the process comprising the steps of applying to the substrate surface a material comprising one or more reactive sites having an activated ethylenically unsaturated double bond of the formula I: Wherein, if n= 0, m= 1, X 3 is carbon and X 1 is nitrogen, the bond between them being triple, and X 4 does not exist; if n= 1, m= 1, X 3 is carbon and X 1 is oxygen or sulphur, the bond between them being double, and X 2 is oxygen or sulphur or nitrogen substituted by an organo substituent; if n= 1, m= 1 or 2, X 3 is sulphur and X 1 is oxygen, the bond between them being double, and X 2 is nitrogen substituted by an organo substituent; R 1 , R 2 , each independently represent hydrogen, hydroxyl or organo group, R 3 and R 4 each independently represent hydrogen or organo group, eventually containing a silicon atom R 1 and R 2 being not linked to R 4 . and, in a further step, of covalently binding the reactive group of the molecular probe to the activated ethylenically unsaturated double bond of the reactive sites having formula I. Preferred activated unsaturated moiet(ies) comprises unsaturated ester moiet(ies) such as (meth)acrylate moiet(ies). Preferred probes comprise amino groups thereon. Preferred strong links are covalent bonds. Preferred linking reactions are Michael addition reactions. The substrate having probes deposed thereon may be used to interact with a target. Other aspects of the invention relate to substrates and multi-reactive systems (such as microarrays) so prepared; uses thereof; and products and information obtained therefrom.
摘要翻译：公开了制备基材的方法。1.制备在其上具有与分子探针的反应性基团反应的位点的基材的方法，所述方法包括以下步骤：将包含具有式I活化的烯属不饱和双键的一个或多个反应性位点：其中，如果n = 0，m = 1，X 3是碳并且X 1是氮，它们之间的键是三重的，并且X 4不存在; 如果n = 1，m = 1，X 3是碳并且X 1是氧或硫，它们之间的键是双键，并且X 2 = 是被有机取代基取代的氧或硫或氮; 如果n = 1，m = 1或2，X 3是硫而X 1是氧，它们之间的键是双键，并且X 2 = 是被有机取代基取代的氮; R 1，R 2各自独立地表示氢，羟基或有机基团，R 3和R 4分别为独立地表示氢或有机基团，最终含有硅原子R 1和R 2不连接到R 4上。并且在进一步的步骤中将分子探针的反应性基团共价结合到具有式I的反应活性部位的活化的烯键式不饱和双键上。优选的活化不饱和醇包括不饱和酯（例如（甲基）丙烯酸酯）丙烯酸酯（酯）。优选的探针在其上包含氨基。优选的强链接是共价键。优选的连接反应是迈克尔加成反应。具有置于其上的探针的衬底可以用于与目标相互作用。本发明的其他方面涉及如此制备的底物和多反应系统（如微阵列）其用途; 以及从中获得的产品和信息。

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