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1. 发明申请

WO2009117689A1 HEMICELLULASE ENRICHED COMPOSITIONS FOR ENHANCING HYDROLYSIS OF BIOMASS 审中-公开
标题翻译：促进生物质水解的致密组合物
公开(公告)号：WO2009117689A1
公开(公告)日：2009-09-24
申请号：PCT/US2009/037853
申请日：2009-03-20
申请人： DANISCO US INC., GENENCOR DIVISION , POWER, Scott D. , CALDWELL, Robert M. , LANTZ, Suzanne E. , LARENAS, Edmund, A.
发明人： POWER, Scott D. , CALDWELL, Robert M. , LANTZ, Suzanne E. , LARENAS, Edmund, A.
IPC分类号： C12N9/24
CPC分类号： C12P19/14 , A61N1/05 , A61N1/0573 , A61N1/086 , A61N1/37 , C12N9/2437 , C12N9/2477 , C12N9/248 , C12P19/02 , C12P19/12 , C12Y301/01072 , C12Y301/01073 , C12Y302/01001 , C12Y302/01003 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01022 , C12Y302/01037 , C12Y302/01055 , C12Y302/01074 , C12Y302/01078 , C12Y302/01091 , C12Y302/01131 , C12Y302/01136 , Y10T29/49117
摘要： Described are compositions and methods relating to cellulase/hemicellulase enzyme blends for improving the enzymatic hydrolysis of cellulosic and hemicellulosic materials, as commonly found in biomass
摘要翻译：描述了与纤维素酶/半纤维素酶共混物相关的组合物和方法，用于改善纤维素和半纤维素材料的酶水解，如通常在生物质

2. 发明申请

WO2005012547A2 MULTIMERIC OXIDOREDUCTASES 审中-公开
标题翻译：多种氧化剂
公开(公告)号：WO2005012547A2
公开(公告)日：2005-02-10
申请号：PCT/US2004024204
申请日：2004-07-27
申请人： GENENCOR INT , CALDWELL ROBERT M , RASHID M HARUNUR , VALLE FERNANDO
发明人： CALDWELL ROBERT M , RASHID M HARUNUR , VALLE FERNANDO
IPC分类号： C07H21/04 , C12N9/02 , C12N9/06 , C12Q
CPC分类号： C12P7/58 , C12N9/0004 , C12N9/002 , C12N15/74 , C12N15/78 , C12P7/60
摘要： The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.
摘要翻译：本发明涉及在碳底物的酶促转化中起作用的多聚体氧化还原酶复合物，所述复合物具有脱氢酶亚基和细胞色素C亚基。本发明还涉及编码多聚体复合物的多核苷酸及其使用方法。

3. 发明申请

WO2006074005A2 NOVEL VARIANT HYPOCREA JECORINA CBH2 CELLULASES 审中-公开
标题翻译：新型变异胶原蛋白CBH2细胞
公开(公告)号：WO2006074005A2
公开(公告)日：2006-07-13
申请号：PCT/US2005/047266
申请日：2005-12-22
申请人： GENENCOR INTERNATIONAL, INC. , AEHLE, Wolfgang , CALDWELL, Robert, M. , DANKMEYER, Lydia , GOEDEGEBUUR, Frits , KELEMEN, Bradley, R. , MITCHINSON, Colin , NEEFE, Paulien , TEUNISSEN, Pauline
发明人： AEHLE, Wolfgang , CALDWELL, Robert, M. , DANKMEYER, Lydia , GOEDEGEBUUR, Frits , KELEMEN, Bradley, R. , MITCHINSON, Colin , NEEFE, Paulien , TEUNISSEN, Pauline
IPC分类号： C12N9/42 , C11D3/386 , A23K1/165 , C12P7/10
CPC分类号： C11D3/38645 , C12N9/2437 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
摘要翻译：本文描述了H.cecorina CBH2，Cel6A酶的变体。本发明提供了具有改变的热稳定性的新型纤维二糖水解酶。

4. 发明申请

WO2004060909A3 INCREASING PRODUCTION OF PROTEINS IN GRAM-POSITIVE MICROORGANISMS 审中-公开
标题翻译：增加蛋白质在生物微生物中的生产
公开(公告)号：WO2004060909A3
公开(公告)日：2005-11-03
申请号：PCT/US0337277
申请日：2003-11-17
申请人： GENENCOR INT , CALDWELL ROBERT M , QUAX WILHELMUS J
发明人： CALDWELL ROBERT M , QUAX WILHELMUS J
IPC分类号： C07K14/32 , C12N1/21 , C12N15/31 , C12N15/75 , C12P21/02 , C12P21/06
CPC分类号： C07K14/32 , C07K2319/00 , C12N15/75 , C12P21/02
摘要： The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis secretion factor SecG. The present invention also provides means for increasing the secretion of heterologous or homologous proteins in Gram-positive microorganisms.
摘要翻译：本发明涉及革兰氏阳性微生物中的分泌。本发明提供枯草芽孢杆菌分泌因子SecG的核酸和氨基酸序列。本发明还提供增加革兰氏阳性微生物中异源或同源蛋白质分泌的方法。

5. 发明申请

WO0234762A3 METHOD FOR GENERATING A LIBRARY OF OLIGONUCLEOTIDES COMPRISING A CONTROLLED DISTRIBUTION OF MUTATIONS 审中-公开
标题翻译：用于产生包含可控制分布的寡核苷酸的图书馆的方法
公开(公告)号：WO0234762A3
公开(公告)日：2003-03-13
申请号：PCT/US0132046
申请日：2001-10-12
申请人： GENENCOR INT , CALDWELL ROBERT M , SCHELLENBERGER VOLKER
发明人： CALDWELL ROBERT M , SCHELLENBERGER VOLKER
IPC分类号： C07B61/00 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/1031 , C12N15/102 , C12N15/1027 , C40B40/00
摘要： Methods are disclosed for producing libraries of nucleic acid molecules which libraries are derived from a nucleic acid template. The libraries comprise variant nucleic acids which are produced from a mutagenesis strategy using, e.g., a plurality of defined mutagenic and/or non-mutagenic primers and specific reaction conditions which favor the production of varied combinatorial mutants.
摘要翻译：公开了用于产生文库来自核酸模板的核酸分子文库的方法。文库包含使用例如多个确定的诱变和/或非诱变引物和有利于产生不同组合突变体的特异性反应条件的诱变策略产生的变体核酸。

6. 发明申请

WO9920727A9 CLEANING COMPOSITIONS CONTAINING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 审中-公开
标题翻译：含有多取代蛋白质变体的清洁组合物
公开(公告)号：WO9920727A9
公开(公告)日：1999-09-02
申请号：PCT/US9822588
申请日：1998-10-23
申请人： PROCTER & GAMBLE , GENENCOR INT , GHOSH CHANCHAL KUMAR , BAECK ANDRE CESAR , OHTANI RYOHEI , BUSCH ALFRED , SHOWELL MICHAEL STANFORD , SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , COLLIER KATHERINE D , CALDWELL ROBERT M , POULOSE AYROOKARAN J
发明人： GHOSH CHANCHAL KUMAR , BAECK ANDRE CESAR , OHTANI RYOHEI , BUSCH ALFRED , SHOWELL MICHAEL STANFORD , SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , COLLIER KATHERINE D , CALDWELL ROBERT M , POULOSE AYROOKARAN J
IPC分类号： A23G4/00 , A23G4/12 , A61K8/66 , A61Q11/00 , C11D1/74 , C11D3/37 , C11D3/386 , C12N9/54 , A23G3/30 , A61K7/28 , A61K7/48
CPC分类号： A23G4/123 , A61K8/66 , A61Q11/00 , C11D1/74 , C11D3/3776 , C11D3/386 , C12N9/54 , C12Y304/21062
摘要： The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of DOLLAR (Bacillus amyloliquefaciens) subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
摘要翻译：本发明涉及包含蛋白酶变体的清洁组合物。一种清洁组合物包含蛋白酶变体，其包括氨基酸残基与对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置103的氨基酸残基处的另一个天然存在的氨基酸残基的取代，以及氨基酸残基与另一天然存在的一个或多个对应于1,2,3,4,8,9,10,12,13,16,17,18,19,20,21,22,24,27,33， 37，38，42，43，48，55，57，58，61，62，68，72，75，76，77，78，79，86，87，89，97，98，99，101，102， 104，106，107，109，111，114，116，117，119，121，123，126，128，130，131，133，134，137，140，141，142，146， 160,166,167,170,173,174,177,181,182,183,184,185,188,192,194,198,203,204,205,206,209,210,211,212,213， 214,215,216,217,218,222,224,227,228,222,224,227,228,230,232,236,237,238,240,242,243,244,245,246,247,248,249,251,252,225,216,217,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224 253,254,25 5,256,257,258,259,260,261,262,263,265,268,269,270,271,272,274和275的解淀粉芽孢杆菌枯草杆菌蛋白酶; 其中当所述蛋白酶变体包括对位置103和76的氨基酸残基置换时，还有一个或多个除对应于位置27,99的氨基酸残基位置之外的氨基酸残基的氨基酸残基的取代，解淀粉芽孢杆菌枯草杆菌蛋白酶的101,104,107,109,123,128,166,204,206,210,216,217,218,222,260,265或274; 和一种或多种清洁辅助材料。另一种清洁组合物包含蛋白酶变体，其包括氨基酸残基与对应于DOLLAR（解淀粉芽孢杆菌）的位置62,212,230,232,252和257的一个或多个氨基酸残基处的另一个天然存在的氨基酸残基取代，枯草杆菌蛋白酶; 以及一种或多种清洁辅助材料。还提供了使用清洁组合物的方法。

7. 发明申请

WO2005012547A3 MULTIMERIC OXIDOREDUCTASES 审中-公开
公开(公告)号：WO2005012547A3
公开(公告)日：2005-02-10
申请号：PCT/US2004/024204
申请日：2004-07-27
申请人： GENENCOR INTERNATIONAL, INC. , CALDWELL, Robert, M. , RASHID, M., Harunur , VALLE, Fernando
发明人： CALDWELL, Robert, M. , RASHID, M., Harunur , VALLE, Fernando
IPC分类号： C12N9/00 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/34 , C12Q1/68 , C12P21/06 , A61K38/44 , C07H21/04 , C07K1/00 , C07K16/00 , C12P21/04
摘要： The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.

8. 发明申请

WO2006074005A3 VARIANT HYPOCREA JECORINA CBH2 CELLULASES 审中-公开
标题翻译：变种高锰酸钾CBH2细胞
公开(公告)号：WO2006074005A3
公开(公告)日：2006-11-16
申请号：PCT/US2005047266
申请日：2005-12-22
申请人： GENENCOR INT , AEHLE WOLFGANG , CALDWELL ROBERT M , DANKMEYER LYDIA , GOEDEGEBUUR FRITS , KELEMEN BRADLEY R , MITCHINSON COLIN , NEEFE PAULIEN , TEUNISSEN PAULINE
发明人： AEHLE WOLFGANG , CALDWELL ROBERT M , DANKMEYER LYDIA , GOEDEGEBUUR FRITS , KELEMEN BRADLEY R , MITCHINSON COLIN , NEEFE PAULIEN , TEUNISSEN PAULINE
IPC分类号： C12N9/42 , A23K1/165 , C11D3/386 , C12P7/10 , D06L4/40
CPC分类号： C11D3/38645 , C12N9/2437 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
摘要翻译：本文描述了H.cecorina CBH2，Cel6A酶的变体。本发明提供了具有改变的热稳定性的新型纤维二糖水解酶。

9. 发明申请

WO2004060909A2 INCREASING PRODUCTION OF PROTEINS IN GRAM-POSITIVE MICROORGANISMS 审中-公开
标题翻译：增加蛋白质在生物微生物中的生产
公开(公告)号：WO2004060909A2
公开(公告)日：2004-07-22
申请号：PCT/US2003/037277
申请日：2003-11-17
申请人： GENENCOR INTERNATIONAL, INC. , CALDWELL, Robert, M. , QUAX, Wilhelmus, J.
发明人： CALDWELL, Robert, M. , QUAX, Wilhelmus, J.
IPC分类号： C07K
CPC分类号： C07K14/32 , C07K2319/00 , C12N15/75 , C12P21/02
摘要： The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis secretion factor SecG. The present invention also provides means for increasing the secretion of heterologous or homologous proteins in Gram-positive microorganisms.
摘要翻译：本发明涉及革兰氏阳性微生物中的分泌。本发明提供枯草芽孢杆菌分泌因子SecG的核酸和氨基酸序列。本发明还提供增加革兰氏阳性微生物中异源或同源蛋白质分泌的方法。

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