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81. 发明申请

WO2005121321A2 COMPOSITIONS COMPRISING FEMALE GERMLINE STEM CELLS AND METHODS OF USE THEREOF 审中-公开
标题翻译：包含雌性GERMLINE干细胞的组合物及其使用方法
公开(公告)号：WO2005121321A2
公开(公告)日：2005-12-22
申请号：PCT/US2005017221
申请日：2005-05-17
申请人： GEN HOSPITAL CORP , TILLY JONATHAN L , JOHNSON JOSHUA
发明人： TILLY JONATHAN L , JOHNSON JOSHUA
IPC分类号： A61K35/12 , C12N5/074 , C12N5/06
CPC分类号： C12N5/0611 , A61B17/43 , A61K35/14 , A61K35/28 , A61K2035/124 , C12N5/0609 , C12N5/0634 , C12N2506/11 , C12P21/06
摘要： The present invention relates to female germline stem cells and their progenitors, methods of isolation thereof, and methods of use thereof.
摘要翻译：本发明涉及雌性生殖系干细胞及其祖细胞，其分离方法及其使用方法。

82. 发明申请

WO2005041942A2 PREPARATION OF THREE-DIMENSIONAL MAMMALIAN OVARIAN FOLLICULAR CELL AND OVARIAN FOLLICLE CULTURE SYSTEMS IN A BIOCOMPATIBLE MATRIX 审中-公开
标题翻译：生物相容性基质制备三维哺乳动物卵巢卵泡和卵巢卵囊培养系统
公开(公告)号：WO2005041942A2
公开(公告)日：2005-05-12
申请号：PCT/EP2004/012384
申请日：2004-11-02
申请人： UNIVERSITA' DEGLI STUDI DI MILANO , UNIVERSITA' DEGLI STUDI DI PAVIA , UNIVERSITA' DEGLI STUDI DI BOLOGNA , VIGO, Daniele , RUSSO, Vincenzo , FAUSTINI, Massimo , STACCHEZZINI, Simona , CONTE, Ubaldo , TORRE, Maria, Luisa , ACCORSI, Pier, Attilio , GALEATI, Giovanna , SPINACI, Marcella
发明人： VIGO, Daniele , RUSSO, Vincenzo , FAUSTINI, Massimo , STACCHEZZINI, Simona , CONTE, Ubaldo , TORRE, Maria, Luisa , ACCORSI, Pier, Attilio , GALEATI, Giovanna , SPINACI, Marcella
IPC分类号： A61K9/50
CPC分类号： C12N5/0609 , A61K9/5036 , A61K35/48 , C12N5/0012 , C12N2502/243 , C12N2533/74
摘要： A process for encapsulating and immobilising mammalian stem cells, ovarian follicular cells, gametes, ovarian follicles or mammalian embryos which are able to auto-organise into three-dimensional structures in vitro , and express biological functions in a manner similar to that which is observed in the organism in vivo is described. The capsules are constituted by: a nucleus containing stem cells, ovarian follicular cells, gametes, ovarian follicles or mammalian embryos and/or a biocompatible and/or biodegradable polymer; a semi-permeable membrane constituted by a divalent or trivalent metal ion salt of alginic acid, optionally cross-linked on the inner and/or outer surface and/or on both surfaces, optionally vehicularising a second or more cellular species. The cells and follicles prepared and cultivated using this methodology are used for the in vitro and/or in vivo production of peptides, proteins, antibodies, hormones and hormone precursors, metabolites and catabolites typical of these cellular structures, plasma membranes, nuclear membranes, cytoplasmic organelles, somatic cell nuclei or gametes and embryos.
摘要翻译：一种用于封装和固定哺乳动物干细胞，卵巢滤泡细胞，配子，卵巢滤泡或哺乳动物胚胎的方法，其能够在体外自动组织成三维结构体并且以与在生物体内观察到的相似的方式表达生物功能。胶囊由含有干细胞的细胞核，卵巢滤泡细胞，配子，卵巢滤泡或哺乳动物胚胎和/或生物相容和/或生物可降解聚合物构成; 由藻酸的二价或三价金属离子盐构成的半透膜，任选在内表面和/或外表面和/或两个表面上交联，任选地使第二种或更多种细胞物种交联。使用该方法制备和培养的细胞和滤泡用于体外和/或体内生产肽，蛋白质，抗体，激素和激素前体，代谢物和典型的这些细胞结构的分解代谢物，质膜，核膜，细胞质细胞器，体细胞核或配子和胚胎。

83. 发明申请

WO2003046129A2 COMPOSITIONS FOR THE DERIVATION OF GERM CELLS FROM STEM CELLS AND METHODS OF USE THEREOF 审中-公开
标题翻译：用于从干细胞衍生的细胞的组合物及其使用方法
公开(公告)号：WO2003046129A2
公开(公告)日：2003-06-05
申请号：PCT/US2002/036260
申请日：2002-11-12
申请人： TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA , SCHOLER, Hans, R. , HUEBNER, Karin, M.
发明人： SCHOLER, Hans, R. , HUEBNER, Karin, M.
IPC分类号： C12N
CPC分类号： C12N15/873 , C07K14/4702 , C12N5/0609 , C12N15/85 , C12N15/877 , C12N2501/115 , C12N2501/119 , C12N2501/125 , C12N2501/235 , C12N2502/02 , C12N2506/02 , C12N2510/00 , C12N2517/04 , C12N2830/008 , C12N2830/85 , G01N33/5014
摘要： Compositions and methods are provided for the reproducible derivation of germ cells and oocytes and spermatogonia therefrom. Also provide are methods of use of the same in reproductive and therapeutic cloning protocols.
摘要翻译：提供组合物和方法用于从其中可重复地衍生生殖细胞和卵母细胞和精原细胞。还提供了在生殖和治疗克隆方案中使用它们的方法。

84. 发明申请

WO0188098A3 FERTILIZATION OF AGED OOCYTES 审中-公开
标题翻译：老化的化学品的加工
公开(公告)号：WO0188098A3
公开(公告)日：2002-05-16
申请号：PCT/EP0105285
申请日：2001-05-09
申请人： SCHERING AG , NOVO NORDISK AS , CUKURCAM SUNA , HEGELE HARTUNG CHRISTA , BLUME THORSTEN , ESPERLING PETER
发明人： CUKURCAM SUNA , HEGELE-HARTUNG CHRISTA , BLUME THORSTEN , ESPERLING PETER
IPC分类号： A01K67/02 , A61K31/00 , A61K31/575 , A61K35/54 , A61K38/18 , A61K38/27 , A61P15/08 , C12N5/075 , G01N33/50 , C12N5/06 , C12N5/08
CPC分类号： C12N5/0609 , A61K31/00 , A61K31/575 , C12N2501/11 , C12N2501/392 , C12N2501/70 , C12N2517/10
摘要： The present invention relates to the use of FF-MAS and/or MAS analogues for the fertilization of aged oocytes.
摘要翻译：本发明涉及FF-MAS和/或MAS类似物用于老化卵母细胞受精的用途。

85. 发明申请

WO0176360A3 SYNCHRONIZATION OF THE CYTOPLASMATIC AND THE NUCLEAR MATURATION OF OOCYTES IN VITRO 审中-公开
标题翻译：细胞色素的同步化和体外成分的核苷酸的同步
公开(公告)号：WO0176360A3
公开(公告)日：2002-01-24
申请号：PCT/DK0100239
申请日：2001-04-06
申请人： NOVO NORDISK AS , SCHERING AG , ANDERSEN CLAUS YDING , BYSKOV ANNE GRETE
发明人： ANDERSEN CLAUS YDING , BYSKOV ANNE GRETE
IPC分类号： A61K35/48 , A61P15/08 , C12N5/075 , C12N5/08 , A61K38/24
CPC分类号： C12N5/0609
摘要： The present invention relates to a method for in vitro maturation of oocytes comprising the steps of: (a) culturing one or more GV oocytes in a culture medium, the culture medium comprising a nuclear maturation inhibiting substance and comprising one or more gonadotropins and/or one or more growth factors, the culturing taking place for a time period sufficient for cytoplasmatic maturation to occur; (b) washing the GV oocytes of step (a) to remove the nuclear maturation inhibiting substance; (c) culturing the washed oocytes of step (b) in a culture medium comprising one or more gonadotropins and/or one or more growth factors and/or MAS for a time period sufficient for nuclear maturation. The invention also relates to an oocyte culture medium comprising a nuclear maturation inhibiting substance and comprising one or more gonadotropins and/or one or more growth factors. The nuclear maturation inhibiting substance may be a MAS antagonist or an FF-MAS synthesis inhibitor, preferably a cytochrome P450 Panosterol 14 alpha -demethylase (P45014DM) inhibitor e.g. ketoconazole or 22-hydroxycholesterol. The one or more gonadotropins and/or one or more growth factors are preferably a combination of EGF and FSH and/or LH. The invention also relates to the use of a nuclear maturation inhibiting substance and one or more gonadotropins and/or one or more growth factors as described above for the preparation of a cell culture medium for in vitro maturation of oocytes.
摘要翻译：本发明涉及卵母细胞体外成熟的方法，其包括以下步骤：（a）在培养基中培养一种或多种GV卵母细胞，所述培养基包含核成熟抑制物质，并且包含一种或多种促性腺激素和/或一种或多种生长因子，培养发生足以发生细胞质成熟的时间段; （b）洗涤步骤（a）的GV卵母细胞以去除核成熟抑制物质; （c）在包含一种或多种促性腺激素和/或一种或多种生长因子和/或MAS的培养基中培养步骤（b）的洗涤的卵母细胞足以进行核成熟的时间段。本发明还涉及包含核成熟抑制物质并包含一种或多种促性腺激素和/或一种或多种生长因子的卵母细胞培养基。核成熟抑制物质可以是MAS拮抗剂或FF-MAS合成抑制剂，优选细胞色素P450 Panosterol14α-二甲基化酶（P45014DM）抑制剂。酮康唑或22-羟基胆固醇。一种或多种促性腺激素和/或一种或多种生长因子优选是EGF和FSH和/或LH的组合。本发明还涉及如上所述的核成熟抑制物质和一种或多种促性腺激素和/或一种或多种生长因子用于制备卵母细胞体外成熟的细胞培养基的用途。

86. 发明申请

WO01076360A2 SYNCHRONIZATION OF THE CYTOPLASMATIC AND THE NUCLEAR MATURATION OF OOCYTES IN VITRO 审中-公开
标题翻译：细胞色素的同步化和体外成分的核苷酸的同步
公开(公告)号：WO01076360A2
公开(公告)日：2001-10-18
申请号：PCT/DK2001/000239
申请日：2001-04-06
IPC分类号： A61K35/48 , A61P15/08 , C12N5/075 , A01K67/027
CPC分类号： C12N5/0609
摘要： The present invention relates to a method for in vitro maturation of oocytes comprising the steps of: (a) culturing one or more GV oocytes in a culture medium, the culture medium comprising a nuclear maturation inhibiting substance and comprising one or more gonadotropins and/or one or more growth factors, the culturing taking place for a time period sufficient for cytoplasmatic maturation to occur; (b) washing the GV oocytes of step (a) to remove the nuclear maturation inhibiting substance; (c) culturing the washed oocytes of step (b) in a culture medium comprising one or more gonadotropins and/or one or more growth factors and/or MAS for a time period sufficient for nuclear maturation. The invention also relates to an oocyte culture medium comprising a nuclear maturation inhibiting substance and comprising one or more gonadotropins and/or one or more growth factors. The nuclear maturation inhibiting substance may be a MAS antagonist or an FF-MAS synthesis inhibitor, preferably a cytochrome P450 Panosterol 14 alpha -demethylase (P45014DM) inhibitor e.g. ketoconazole or 22-hydroxycholesterol. The one or more gonadotropins and/or one or more growth factors are preferably a combination of EGF and FSH and/or LH. The invention also relates to the use of a nuclear maturation inhibiting substance and one or more gonadotropins and/or one or more growth factors as described above for the preparation of a cell culture medium for in vitro maturation of oocytes.
摘要翻译：本发明涉及卵母细胞体外成熟的方法，其包括以下步骤：（a）在培养基中培养一种或多种GV卵母细胞，所述培养基包含核成熟抑制物质，并且包含一种或多种促性腺激素和/或一种或多种生长因子，培养发生足以发生细胞质成熟的时间段; （b）洗涤步骤（a）的GV卵母细胞以去除核成熟抑制物质; （c）在包含一种或多种促性腺激素和/或一种或多种生长因子和/或MAS的培养基中培养步骤（b）的洗涤的卵母细胞足以进行核成熟的时间段。本发明还涉及包含核成熟抑制物质并包含一种或多种促性腺激素和/或一种或多种生长因子的卵母细胞培养基。核成熟抑制物质可以是MAS拮抗剂或FF-MAS合成抑制剂，优选细胞色素P450 Panosterol14α-二甲基化酶（P45014DM）抑制剂。酮康唑或22-羟基胆固醇。一种或多种促性腺激素和/或一种或多种生长因子优选是EGF和FSH和/或LH的组合。本发明还涉及如上所述的核成熟抑制物质和一种或多种促性腺激素和/或一种或多种生长因子用于制备卵母细胞体外成熟的细胞培养基的用途。

87. 发明申请

WO01070261A1 SPERM FACTOR OSCILLOGENIN 审中-公开
标题翻译： SPERM因子OSCILLOGENIN
公开(公告)号：WO01070261A1
公开(公告)日：2001-09-27
申请号：PCT/US2001/008998
申请日：2001-03-21
IPC分类号： C12N15/09 , A61K35/12 , A61K38/00 , A61K45/00 , A61P15/08 , A61P15/16 , A61P43/00 , C07K14/47 , C07K16/18 , C12N5/075 , C12P21/08 , C12Q1/68 , A61K39/00 , A23J1/00 , C07H21/04 , C07K16/00 , C12P21/06 , C12Q1/00
CPC分类号： C07K14/4705 , A61K35/12 , A61K38/00 , C12N5/0609 , C12N2500/14 , C12N2501/70 , C12N2517/10
摘要： The specification describes a novel compound, oscillogenin, which is an active agent in furthering oocyte fertilization by sperm or in parthenogenetic activation of an oocyte. The specification discloses methods of isolating oscillogenin to modulate fertility and to enhance parthenogenetic activation of oocytes for nuclear transfer or in ICSI procedures, and methods of using oscillogenin to test amounts of it in sperm and thus sperm fertility.
摘要翻译：该说明书描述了一种新颖的化合物，其是促进精子卵母细胞受精或卵母细胞孤雌激活的活性剂。该说明书公开了隔离发光蛋白以调节生殖力和增强核转移或ICSI程序中卵母细胞的孤雌激活的方法，以及使用依曲麦甜蛋白测量精子量并因此测定精子生殖力的方法。

88. 发明申请

WO00021555A1 CONTRACEPTION THROUGH ANTAGONIZING WNT; OOCYTE MATURATION WITH WNT POLYPEPTIDE 审中-公开
标题翻译：通过拮抗WNT的承诺; 用WNT多糖制成的OOCYTE成熟
公开(公告)号：WO00021555A1
公开(公告)日：2000-04-20
申请号：PCT/US1999/023640
申请日：1999-10-13
IPC分类号： A61K38/17 , A61P15/00 , A61P15/18 , C12N5/075 , A61K38/18 , C07K16/22 , C12N5/06 , C12N5/08
CPC分类号： A61K38/18 , A61K38/177 , A61K38/179 , A61K39/0006 , C07K16/22 , C12N5/0609 , C12N2501/415 , C12N2517/10
摘要： A method of contraception in which a Wnt antagonist is administered to a female primate to inhibit oocyte development. Among antagonists, antibody binding to a Wnt polypeptide and inhibing oocyte development. A method of promoting maturation/enhancing viability of an oocyte, increasing the number of mature oocytes.
摘要翻译：一种避孕方法，其中向雌性灵长类动物施用Wnt拮抗剂以抑制卵母细胞发育。在拮抗剂中，抗体与Wnt多肽结合并抑制卵母细胞发育。促进卵母细胞成熟/增强活力的方法，增加成熟卵母细胞的数量。

89. 发明申请

WO9952544A3 REGULATION OF OVARIAN MATURATION AND FUNCTION USING EPIDERMAL GROWTH FACTOR 审中-公开
标题翻译：使用生长发育因子调控卵巢成熟和功能
公开(公告)号：WO9952544A3
公开(公告)日：2000-01-20
申请号：PCT/US9908192
申请日：1999-04-14
申请人： UNIV TEXAS TECH , LEE VAUGHAN H , MCGLONE JOHN J
发明人： LEE VAUGHAN H , MCGLONE JOHN J
IPC分类号： A61K38/22 , A61K38/18 , A61P15/08 , C12N5/075
CPC分类号： A61K38/1808 , C12N5/0609 , C12N2501/11 , C12N2517/10
摘要： A method is provided for regulating vertebrate ovarian maturation and function using growth factors by providing an amount of epidermal growth factor to prepubertal ovaries of female vertebrate species including stimulation of primordial follicles and enhancing activation of dormant follicles with the results that the mechanics of the method regulate early development of ovarian follicles. The method provides activation of dormant follicles and early ovarian maturation which is accelerated to the point of producing earlier breeding cycles as well as increasing first litter sizes. The method for regulating vertebrate/mammal ovulation maturation is also directed to increasing the ovulation rate and increasing litter size at an age of normal breeding.
摘要翻译：提供了一种方法，用于通过向雌性脊椎动物物种的青春前期卵巢提供一定量的表皮生长因子来调节脊椎动物卵巢成熟和功能，包括刺激原始卵泡并增强休眠卵泡的活化，结果该方法的力学调节卵泡早期发育。该方法提供休眠卵泡的活化和早期卵巢成熟，其加速到产生较早的育种周期以及增加第一窝仔的大小。用于调节脊椎动物/哺乳动物排卵成熟的方法也针对在正常育种时期增加排卵率和增加胎仔数量。

90. 发明申请

WO1995003398A1 ENUCLEATION OF OOCYTES 审中-公开
标题翻译：植物油的提炼
公开(公告)号：WO1995003398A1
公开(公告)日：1995-02-02
申请号：PCT/AU1994000411
申请日：1994-07-22
申请人： MONASH UNIVERSITY , TATHAM, Brendan, George
发明人： MONASH UNIVERSITY
IPC分类号： C12N05/06
CPC分类号： A01K67/0275 , A01K2217/05 , A61K35/54 , C12N5/0609 , C12N2500/32 , C12N2517/10
摘要： A process for enucleating oocytes is described. The process includes: providing a source of oocytes; exposing the oocytes to a density gradient to form an oocyte gradient mix; subjecting the oocyte gradient mix to centrifugation to separate an oocyte fraction including enucleated oocytes; and identifying and isolating the enucleated oocytes. Enucleated oocytes produced according to the process have a reduced lipid content compared to enucleated oocytes prepared by conventional methods. They are also capable of electrofusion at amplitudes of 2 kV/cm or less. Enucleated oocytes produced according to the process may be used to prepare nuclear transplantation embryos, which may in turn be used to prepare multiple genetically similar animals.
摘要翻译：描述了摘除卵母细胞的过程。该过程包括：提供卵母细胞源; 将卵母细胞暴露于密度梯度以形成卵母细胞梯度混合物; 对卵母细胞梯度混合物进行离心以分离包括去核卵母细胞的卵母细胞; 并鉴定和分离去核卵母细胞。与通过常规方法制备的去核卵母细胞相比，根据该方法产生的去核卵母细胞的脂质含量降低。它们也能够以2kV / cm或更小的振幅进行电熔。根据该方法产生的去核卵母细胞可用于制备核移植胚，其可以依次用于制备多种遗传相似的动物。

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