专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1995003398A1 ENUCLEATION OF OOCYTES 审中-公开
标题翻译：植物油的提炼
公开(公告)号：WO1995003398A1
公开(公告)日：1995-02-02
申请号：PCT/AU1994000411
申请日：1994-07-22
申请人： MONASH UNIVERSITY , TATHAM, Brendan, George
发明人： MONASH UNIVERSITY
IPC分类号： C12N05/06
CPC分类号： A01K67/0275 , A01K2217/05 , A61K35/54 , C12N5/0609 , C12N2500/32 , C12N2517/10
摘要： A process for enucleating oocytes is described. The process includes: providing a source of oocytes; exposing the oocytes to a density gradient to form an oocyte gradient mix; subjecting the oocyte gradient mix to centrifugation to separate an oocyte fraction including enucleated oocytes; and identifying and isolating the enucleated oocytes. Enucleated oocytes produced according to the process have a reduced lipid content compared to enucleated oocytes prepared by conventional methods. They are also capable of electrofusion at amplitudes of 2 kV/cm or less. Enucleated oocytes produced according to the process may be used to prepare nuclear transplantation embryos, which may in turn be used to prepare multiple genetically similar animals.
摘要翻译：描述了摘除卵母细胞的过程。该过程包括：提供卵母细胞源; 将卵母细胞暴露于密度梯度以形成卵母细胞梯度混合物; 对卵母细胞梯度混合物进行离心以分离包括去核卵母细胞的卵母细胞; 并鉴定和分离去核卵母细胞。与通过常规方法制备的去核卵母细胞相比，根据该方法产生的去核卵母细胞的脂质含量降低。它们也能够以2kV / cm或更小的振幅进行电熔。根据该方法产生的去核卵母细胞可用于制备核移植胚，其可以依次用于制备多种遗传相似的动物。

IPRDB

热门服务

关于我们

友情链接

联系方式