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61. 发明申请

WO2014092960A1 TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF 审中-公开
标题翻译：表达来自青蒿素的谷氨酰胺酶的TRICHODERMA REESEI HOST细胞及其使用方法
公开(公告)号：WO2014092960A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/071154
申请日：2013-11-21
申请人： DANISCO US INC.
发明人： GE, Jing , HUA, Ling , LEE, Sung Ho , LI, Jalsen , SHETTY, Jayarama, K. , TANG, Zhongmei , ZHANG, Bo , ZHONG, Kun
IPC分类号： C12N1/14 , C12N9/34 , C12N15/80 , C12P19/14
CPC分类号： C12P19/14 , C12N9/2428 , C12P19/02 , C12Y302/01003 , Y02E50/17
摘要： Fungal glucoamylases from Aspergillus fumigatus - expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus . AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus . AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
摘要翻译：提供来自烟曲霉的真菌葡糖淀粉酶 - 在里氏木霉宿主细胞（AfGATR）中表达。里氏木霉宿主细胞以更高或至少相当的水平将AfGATR表达为天然表达的AfGA烟曲霉（Aspergillus fumigatus）。包括AfGA1TR和AfGA2TR的AfGATR在升高的温度和低pH下表现出高活性，因此在α-淀粉酶例如曲霉曲霉α-淀粉酶（AkAA）的存在下，可以有效地使用AfGATR。与由烟曲霉中表达的黑曲霉葡糖淀粉酶（AnGA）或天然AfGA催化的糖化产物相比，AfGATR有利地将淀粉糖化催化剂显着富集DP1（即葡萄糖）的寡糖组合物。 AfGATR如AfGA1TR，AfGA2TR或其变体可以以比AnGA和天然表达的AfGA更低的剂量使用以产生相当水平的葡萄糖。

62. 发明申请

WO2014088935A3 COMPOSITIONS OF BETA-MANNANASE AND METHODS OF USE 审中-公开
公开(公告)号：WO2014088935A3
公开(公告)日：2014-06-12
申请号：PCT/US2013/072576
申请日：2013-12-02
申请人： DANISCO US INC.
发明人： HUA, Ling , LAU, Rosalyn , LE, Steven , QIAN, Zhen , YU, Zheyong
IPC分类号： C07K14/32 , C12N9/24
摘要： The present compositions and methods relate to a beta-mannanase from Bacillus licheniformis , polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).

63. 发明申请

WO2014088934A1 COMPOSITIONS AND METHODS OF USE 审中-公开
标题翻译：组合物和使用方法
公开(公告)号：WO2014088934A1
公开(公告)日：2014-06-12
申请号：PCT/US2013/072572
申请日：2013-12-02
申请人： DANISCO US INC.
发明人： HUA, Ling , LAU, Rosalyn , LE, Steven , QIAN, Zhen , YU, Zheyong
IPC分类号： C12N9/24
CPC分类号： C12P19/14 , C12N9/2437 , C12N9/2494 , C12P19/02 , C12P2203/00 , C12Y302/01004 , C12Y302/01078
摘要： The present compositions and methods relate to a beta-mannanase from Paenibacillus kribbensis, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).
摘要翻译：本发明的组合物和方法涉及来自柯氏芽孢杆菌的β-甘露聚糖酶，编码β-甘露聚糖酶的多核苷酸，及其制备和/或使用方法。含有β-甘露聚糖酶的制剂适用于水解木质纤维素生物质底物，特别是含有可测量水平的半乳甘露聚糖（GGM）和/或葡甘露聚糖（GM）的那些。

64. 发明申请

WO2014071410A1 COMPOSITIONS AND METHODS COMPRISING THERMOLYSIN PROTEASE VARIANTS 审中-公开
标题翻译：包含热变质蛋白酶变体的组合物和方法
公开(公告)号：WO2014071410A1
公开(公告)日：2014-05-08
申请号：PCT/US2013/068590
申请日：2013-11-05
申请人： DANISCO US INC.
发明人： ALEKSEYEV, Viktor Yuryevich , BABE, Lilia Maria , ESTELL, David A. , GOEDEGEBUUR, Frits , MULDER, Harm , TORRES PAZMINO, Daniel Esteban , YAO, Jian , BOTT, Richard, R.
IPC分类号： C12N9/54
CPC分类号： C12N9/54 , C11D3/386 , C12Y304/24027
摘要： The present invention provides serine protease - thermoslysine- variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
摘要翻译：本发明提供了丝氨酸蛋白酶 - 热解丝氨酸变体。具体地，本发明提供了与参考丝氨酸蛋白酶相比具有一个或多个取代的丝氨酸蛋白酶变体。此外，本发明提供包含这些丝氨酸蛋白酶变体的组合物。在一些实施方案中，本发明提供了包含这些丝氨酸蛋白酶变体中的至少一种的清洁组合物。

65. 发明申请

WO2014063097A1 STABILIZATION OF BIOMIMETIC MEMBRANES 审中-公开
标题翻译：生物膜的稳定性
公开(公告)号：WO2014063097A1
公开(公告)日：2014-04-24
申请号：PCT/US2013/065746
申请日：2013-10-18
申请人： DANISCO US INC.
发明人： GIL, Maria Ines Plasencia , KRISTENSEN, Jakob Broberg , MAINZER, Stanley E.
IPC分类号： C07K14/37 , C02F1/44
CPC分类号： B01D71/74 , B01D61/002 , B01D69/144 , B01D71/06 , C02F1/44 , C02F2303/10 , C07K14/245 , C07K14/315 , C07K14/37 , C07K14/415 , Y02W10/30
摘要： The present disclosure provides methods, compositions, kits and apparatuses for stabilizing membranes, membrane proteins, and/or membranes containing membrane proteins using hydrophobin.
摘要翻译：本公开提供了使用疏水蛋白稳定膜，膜蛋白和/或含有膜蛋白的膜的方法，组合物，试剂盒和装置。

66. 发明申请

WO2013166211A3 IDENTIFICATION OF ISOPRENE SYNTHASE VARIANTS WITH IMPROVED PROPERTIES FOR THE PRODUCTION OF ISOPRENE 审中-公开
标题翻译：用于生产异丙酚的具有改进性质的异丙基合成酶变异体的鉴定
公开(公告)号：WO2013166211A3
公开(公告)日：2014-03-20
申请号：PCT/US2013039151
申请日：2013-05-01
申请人： DANISCO US INC , GOODYEAR TIRE & RUBBER
发明人： BECK ZACHARY Q , ESTELL DAVID A , MILLER JEFFREY V , NGAI JAMES , RIFE CHRISTOPHER L , WELLS DEREK H
IPC分类号： C12N9/88
CPC分类号： C12P5/007 , C12N9/88 , C12Y402/03027
摘要： The invention provides for compositions and methods for producing isoprene using isoprene synthase variants with improved properties.
摘要翻译：本发明提供使用具有改进性能的异戊二烯合酶变体产生异戊二烯的组合物和方法。

67. 发明申请

WO2013181647A3 COMPOSITIONS AND METHODS OF PRODUCING ISOPRENE AND/OR INDUSTRRIAL BIO-PRODUCTS USING ANAEROBIC MICROORGANISMS 审中-公开
标题翻译：使用无机微生物生产异丙苯和/或工业生物产品的组合物和方法
公开(公告)号：WO2013181647A3
公开(公告)日：2014-03-13
申请号：PCT/US2013043804
申请日：2013-05-31
申请人： DANISCO US INC , GOODYEAR TIRE & RUBBER
发明人： BECK ZACHARY Q , CERVIN MARGUERITE A , CHOTANI GOPAL K , PERES CAROLINE M , SANFORD KARL J , SCOTCHER MILES C , WELLS DEREK H , WHITED GREGORY M
IPC分类号： C12N9/88 , C12P5/00
CPC分类号： C12P7/42 , C12N1/20 , C12N9/88 , C12N15/52 , C12P5/007 , C12P7/04 , C12P7/065 , C12P7/16 , C12P7/54 , C12P7/58 , C12Y402/03027 , Y02E50/10 , Y02E50/17
摘要： This invention provides for compositions and methods of making and using anaerobic microorganisms with one or more nucleic acids. The microorganisms are capable of producing isoprene and/or industrial bio-products in a substantially oxygen-free culture condition.
摘要翻译：本发明提供了制备和使用厌氧微生物与一种或多种核酸的组合物和方法。微生物能够在基本上无氧的培养条件下产生异戊二烯和/或工业生物产物。

68. 发明申请

WO2013177153A1 TRICHODERMA HYDROPHOBIN PRODUCTION 审中-公开
标题翻译： TRICHODERMA HYDROPHOBIN生产
公开(公告)号：WO2013177153A1
公开(公告)日：2013-11-28
申请号：PCT/US2013/042007
申请日：2013-05-21
申请人： DANISCO US INC.
发明人： WARD, Michael
IPC分类号： C12P21/02 , C07K14/37
CPC分类号： C07K14/37
摘要： The invention relates to a method for maximizing expression of a biosurfactant, such as hydrophobin, in a microorganism, in particular a Trichoderma production host.
摘要翻译：本发明涉及使微生物特别是木霉属生产宿主中的生物表面活性剂如疏水蛋白的表达最大化的方法。

69. 发明申请

WO2009149271A8 PRODUCTION OF GLUCOSE FROM STARCH USING ALPHA-AMYLASES FROM BACILLUS SUBTILIS 审中-公开
标题翻译：使用来自BACILLUS SUBTILIS的ALPHA-AMYLASES从淀粉生产葡萄糖
公开(公告)号：WO2009149271A8
公开(公告)日：2013-11-14
申请号：PCT/US2009046279
申请日：2009-06-04
申请人： DANISCO US INC , CASCAO-PEREIRA LUIS G , CHIN REGINA , CUEVAS WILLIAM A , ESTELL DAVID A , LEE SANG-KYU , PEPSIN MICHAEL J , POWER SCOTT D , RAMER SANDRA W , REQUADT CAROL A , SHAW ANDREW , TOPPOZADA AMR R , WALLACE LOUISE
发明人： CASCAO-PEREIRA LUIS G , CHIN REGINA , CUEVAS WILLIAM A , ESTELL DAVID A , LEE SANG-KYU , PEPSIN MICHAEL J , POWER SCOTT D , RAMER SANDRA W , REQUADT CAROL A , SHAW ANDREW , TOPPOZADA AMR R , WALLACE LOUISE
IPC分类号： C12P19/02 , C11D3/386 , C12N9/28
CPC分类号： C12P19/02 , C11D3/221 , C11D3/386 , C12N9/2417 , D06L1/14 , Y02E50/17
摘要： An alpha amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
摘要翻译：来自枯草芽孢杆菌（AmyE）的α淀粉酶从各种碳水化合物底物产生显着量的葡萄糖，包括植物淀粉，麦芽庚糖和麦芽三糖。除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。

70. 发明申请

WO2013166320A1 LEGUME ISOPRENE SYNTHASE FOR PRODUCTION OF ISOPRENE 审中-公开
标题翻译：用于生产异烟肼的异丙酚合成酶
公开(公告)号：WO2013166320A1
公开(公告)日：2013-11-07
申请号：PCT/US2013/039315
申请日：2013-05-02
申请人： DANISCO US INC. , THE GOODYEAR TIRE & RUBBER COMPANY
发明人： BEATTY, Mary, K. , HAYES, Kevin , HOU, Zhenglin , MEYER, David, J. , NANNAPANENI, Kishore , RIFE, Christopher, L. , WELLS, Derek, H. , ZASTROW-HAYES, Gina, M.
IPC分类号： C07K14/415 , C12N9/88
CPC分类号： C12P5/007 , C07K14/415 , C12N9/88 , C12Q1/527 , C12Y402/03027
摘要： The present invention provides methods and compositions of polypeptides having isoprene synthase activity with improved performance characteristics. In particular, the present invention provides legume isoprene synthases for increased isoprene production in recombinant host cells.
摘要翻译：本发明提供具有改善的性能特征的具有异戊二烯合酶活性的多肽的方法和组合物。特别地，本发明提供用于在重组宿主细胞中增加异戊二烯生产的豆类异戊二烯合酶。

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