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51. 发明申请

WO2006117582A3 EXPORT OF RECOMBINANT PROTEINS BY FLAGELLAR EXPORT APPARATUS 审中-公开
标题翻译：通过出口设备出口重组蛋白
公开(公告)号：WO2006117582A3
公开(公告)日：2007-05-31
申请号：PCT/HU2006000039
申请日：2006-05-02
申请人： PHARMATEST LTD , INST OF ENZYMOLOGY BIOLOG RES , VESZPREM UNIVERSITY , VONDERVISZT FERENC , GAL PETER , VEGH BARBARA , ZAVODSZKY PETER , DOBO JOZSEF
发明人： VONDERVISZT FERENC , GAL PETER , VEGH BARBARA , ZAVODSZKY PETER , DOBO JOZSEF
IPC分类号： C07K14/255 , C12N15/62 , C12P21/00
CPC分类号： C07K14/255 , C07K2319/01 , C07K2319/036
摘要： The invention provides an expression system, wherein the produced recombinant protein is exported by the flagellum specific export apparatus into the outer culture medium. According to the invention the short gene segment of the flagellin gene carrying the recognizing signal of the export apparatus is attached to the gene encoding the protein to be produced. Inserting the so obtained fusion construction into an appropriate carrier vector and expressing it in a suitable host cell, the produced fusion protein is exported by the flagellum specific export apparatus of the host cell. If a segment facilitating the purification and an appropriate specific proteolytic cleavage site is inserted, the expressed recombinant protein can be isolated from the cell culture easily, and then the segments can be removed from the protein to be produced, by digestion. Furthermore, the invention provides fusion proteins carrying a flagellum specific export signal, fusion polynucleotides encoding the fusion proteins, constructs comprising the fusion polynucleotides, vectors and host cells. The invention is applicable in the field of expression of recombinant proteins and their export from host cells.
摘要翻译：本发明提供了一种表达系统，其中所产生的重组蛋白由鞭毛特异性输出装置输出到外培养基中。根据本发明，携带出口设备的识别信号的鞭毛蛋白基因的短基因片段连接到编码待产生蛋白质的基因上。将如此获得的融合构建插入合适的载体载体并在合适的宿主细胞中表达，所产生的融合蛋白由宿主细胞的鞭毛特异性导出装置输出。如果插入促进纯化的片段和适当的特异性蛋白水解切割位点，则可以容易地从细胞培养物中分离出表达的重组蛋白质，然后通过消化将片段从待产生的蛋白质中除去。此外，本发明提供携带鞭毛特异性输出信号的融合蛋白，编码融合蛋白的融合多核苷酸，包含融合多核苷酸，载体和宿主细胞的构建体。本发明适用于重组蛋白的表达及其从宿主细胞的出口。

52. 发明申请

WO2006078657A2 COMPOSITIONS OF PATHOGEN-ASSOCIATED MOLECULAR PATTERNS AND METHODS OF USE 审中-公开
标题翻译：病原相关分子模式的组合物及其使用方法
公开(公告)号：WO2006078657A2
公开(公告)日：2006-07-27
申请号：PCT/US2006/001623
申请日：2006-01-19
申请人： VAXINNATE CORPORATION , POWELL, Thomas, J. , NAKAAR, Valerian , SONG, Langzhou , HULEATT, James, W. , MCDONALD, William, F. , HEWITT, Duane, D.
发明人： POWELL, Thomas, J. , NAKAAR, Valerian , SONG, Langzhou , HULEATT, James, W. , MCDONALD, William, F. , HEWITT, Duane, D.
CPC分类号： A61K39/12 , A61K38/162 , A61K38/164 , A61K39/0258 , A61K39/0275 , A61K2039/6068 , C07K14/005 , C07K14/245 , C07K14/255 , C07K2319/00 , C12N7/00 , C12N2770/24022 , C12N2770/24122 , C12N2770/24134 , Y02A50/386 , Y02A50/388 , Y02A50/39 , Y02A50/394 , Y02A50/396
摘要： Compositions comprise at least a portion of an antigen and at least a portion of a flagellin that lacks a hinge region. Compositions, fusion proteins and polypeptides comprise at least a portion of at least one pathogen-associated molecular pattern and at least a portion of at least one viral protein. The viral protein of the compositions, fusion proteins and polypeptides of the invention are flaviviral proteins, including a West Nile flaviviral protein, a Dengue flaviviral protein, a Langat flaviviral protein, a Kunjin flaviviral protein, a Murray Valley encephalitis flaviviral protein, a Japanese encephalitis flaviviral protein, a Tick- borne encephalitis flaviviral protein, a Yellow fever flaviviral protein and a hepatitis C flaviviral protein. The compositions, fusion proteins and polypeptides are used to stimulate an immune response in a subject.
摘要翻译：组合物包含至少一部分抗原和至少一部分缺乏铰链区的鞭毛蛋白。组合物，融合蛋白质和多肽包含至少一种与病原体相关的分子模式和至少一部分至少一种病毒蛋白的至少一部分。本发明的组合物，融合蛋白和多肽的病毒蛋白是黄病毒蛋白，包括西尼罗河黄病毒蛋白，登革热黄病毒蛋白，兰加特黄病毒蛋白，昆金黄病毒蛋白，墨累谷脑炎黄病毒蛋白，日本脑炎黄病毒蛋白，蜱传脑炎黄病毒蛋白，黄热病黄病毒蛋白和丙型肝炎黄病毒蛋白。组合物，融合蛋白和多肽用于刺激受试者的免疫应答。

53. 发明申请

WO2005064000A1 PROCESS FOR PREPARING L-AMINO ACIDS USING STRAINS OF THE ENTEROBACTERIACEAE FAMILY 审中-公开
标题翻译：使用肠杆菌属菌株制备L-氨基酸的方法
公开(公告)号：WO2005064000A1
公开(公告)日：2005-07-14
申请号：PCT/EP2004/014082
申请日：2004-12-10
申请人： DEGUSSA AG
发明人： DUSCH, Nicole
IPC分类号： C12P13/08
CPC分类号： C07K14/245 , C07K14/25 , C07K14/255 , C12P13/08
摘要： The invention relates to a process for preparing L-amino acids by fermenting recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the desired L-amino acid-producing microorganisms, in which the yaaU ORF, or nucleotide sequences or alleles encoding the gene products, is/are enhanced, in particular overexpressed, is cultured in a medium under conditions under which the desired L-amino acid is enriched in the medium or in the cells, and b) the desired L-amino acid is isolated, with, optionally, constituents of the fermentation broth, and/or the biomass remaining in its/their entirety or in portions (from = 0 to 100%) in the isolated product or being removed completely.
摘要翻译：本发明涉及通过发酵肠杆菌科的重组微生物来制备L-氨基酸的方法，其特征在于：a）所需的L-氨基酸产生微生物，其中yaaU ORF或编码该基因的核苷酸序列或等位基因在培养基中培养产物，特别是过表达的培养基，其中期望的L-氨基酸在培养基或细胞中富集的条件下培养，和b）分离所需的L-氨基酸，任选地，发酵液的成分和/或生物质在其全部或部分（= 0至100％）中残留在分离的产物中或完全除去。

54. 发明申请

WO2005026203A2 DNA PROMOTERS AND ANTHRAX VACCINES 审中-公开
标题翻译： DNA促进剂和ANTHRAX VACCINES
公开(公告)号：WO2005026203A2
公开(公告)日：2005-03-24
申请号：PCT/US2004/030616
申请日：2004-09-20
申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES , KOPECKO, Dennis, J. , OSORIO, Manuel , BHATTACHARYYA, Siba , GIRI, Chandrakant, P. , BLAKE, Milan
发明人： KOPECKO, Dennis, J. , OSORIO, Manuel , BHATTACHARYYA, Siba , GIRI, Chandrakant, P. , BLAKE, Milan
IPC分类号： C07K14/195
CPC分类号： C07K14/32 , A61K39/07 , A61K2039/522 , A61K2039/523 , A61K2039/53 , C07K14/255 , C07K16/1278 , Y02A50/484
摘要： The invention is related to intracellularly induced bacterial DNA promoters and vaccines against Bacillus anthracis .
摘要翻译：本发明涉及细菌内诱导的细菌DNA启动子和针对炭疽芽孢杆菌的疫苗。

55. 发明申请

WO2004013290A3 COMPOSITIONS AND METHODS FOR MOLECULAR BIOLOGY 审中-公开
标题翻译：组合物和分子生物学方法
公开(公告)号：WO2004013290A3
公开(公告)日：2004-07-22
申请号：PCT/US0324064
申请日：2003-08-04
申请人： INVITROGEN CORP
发明人： BYRD DEVON R N , YOUNG ALICE , HARTLEY JAMES L
IPC分类号： C12N15/10 , C12N15/64 , C12N15/66 , C12N15/87 , C07H21/04 , C07K5/00 , C12N15/00 , C12N15/09
CPC分类号： C12N15/63 , C07K14/245 , C07K14/255 , C07K14/32 , C12N15/10 , C12N15/1096 , C12N15/64 , C12N15/66 , C12N15/70 , C12N15/87 , C12Q1/6834
摘要： The present invention provides materials and methods for the utilization of the specific interaction of replication termination sequences with their binding proteins in molecular biology applications.
摘要翻译：本发明提供了在分子生物学应用中利用复制终止序列与其结合蛋白的特异性相互作用的材料和方法。

56. 发明申请

WO03038116A3 SALMONELLA VIRULENCE FACTORS AND USES THEREOF 审中-公开
标题翻译： SALMONELLA VIRULENCE因子及其用途
公开(公告)号：WO03038116A3
公开(公告)日：2003-10-16
申请号：PCT/US0235039
申请日：2002-11-01
申请人： GEN HOSPITAL CORP , AUSUBEL FREDERICK M , ABALLAY ALEJANDRO , DRENKARD ELIANA , MCCORMICK BETH A
发明人： AUSUBEL FREDERICK M , ABALLAY ALEJANDRO , DRENKARD ELIANA , MCCORMICK BETH A
IPC分类号： C07K14/255 , C07H21/04 , A61K39/02 , A61K39/108 , A61K39/112 , C07K1/00 , C12N1/20 , C12N15/00 , C12Q1/68 , G01N33/53
CPC分类号： C07K14/255
摘要： The invention features nucleic acid molecules encoding microbial virulence factors (such as gmhA) and methods of using such gene (and their proteins) as targets to identify anti-pathogenic agents. The invention also features a method of identifying a compound that inhibits the pathogenicity of an effector protein in a nematode, the method involving the steps of: (a) providing a nematode expressing an effector protein; (b) contacting the nematode with a test compound; and (c) determining whether the test compound inhibits the pathogenicity of the effector polypeptide in the nematode.
摘要翻译：本发明的特征在于编码微生物毒力因子（例如gmhA）的核酸分子和使用这种基因（及其蛋白质）作为靶标的方法来鉴定抗病原体。本发明还涉及鉴定抑制线虫中效应蛋白的致病性的化合物的方法，所述方法包括以下步骤：（a）提供表达效应蛋白的线虫; （b）使线虫与测试化合物接触; 和（c）确定测试化合物是否抑制线虫中效应多肽的致病性。

57. 发明申请

WO2003041734A1 SALMONELLA VACCINE 审中-公开
公开(公告)号：WO2003041734A1
公开(公告)日：2003-05-22
申请号：PCT/IB2001/002127
申请日：2001-11-12
申请人： PHARMACIA & UPJOHN COMPANY , LOWERY, David, E. , KENNEDY, Michael, J.
发明人： LOWERY, David, E. , KENNEDY, Michael, J.
IPC分类号： A61K39/112
CPC分类号： C07K14/255
摘要： Attenuated mutant Salmonella bacteria containing inactivated virulence genes are provided for use in vaccines.
摘要翻译：提供含有灭活毒力基因的减毒突变体沙门氏菌用于疫苗。

58. 发明申请

WO02077183A2 IDENTIFICATION OF ESSENTIAL GENES IN MICROORGANISMS 审中-公开
标题翻译：鉴定微生物中的基因
公开(公告)号：WO02077183A2
公开(公告)日：2002-10-03
申请号：PCT/US0209107
申请日：2002-03-21
申请人： ELITRA PHARMACEUTICALS INC , WANG LIANGSU , ZAMUDIO CARLOS , MALONE CHERYL , HASELBECK ROBERT , OHLSEN KARI L , ZYSKIND JUDITH W , WALL DANIEL , TRAWICK JOHN D , CARR GRANT J , YAMAMOTO ROBERT , FORSYTH R ALLYN , XU H HOWARD
发明人： WANG LIANGSU , ZAMUDIO CARLOS , MALONE CHERYL , HASELBECK ROBERT , OHLSEN KARI L , ZYSKIND JUDITH W , WALL DANIEL , TRAWICK JOHN D , CARR GRANT J , YAMAMOTO ROBERT , FORSYTH R ALLYN , XU H HOWARD
IPC分类号： C07K14/195 , C07K14/20 , C07K14/205 , C07K14/21 , C07K14/22 , C07K14/235 , C07K14/24 , C07K14/245 , C07K14/25 , C07K14/255 , C07K14/26 , C07K14/265 , C07K14/285 , C07K14/295 , C07K14/30 , C07K14/31 , C07K14/315 , C07K14/32 , C07K14/33 , C07K14/34 , C07K14/35 , C07K14/355 , C07K14/37 , C07K14/38 , C07K14/40 , C12N
CPC分类号： C07K14/212 , C07K14/195 , C07K14/20 , C07K14/205 , C07K14/21 , C07K14/22 , C07K14/235 , C07K14/24 , C07K14/245 , C07K14/25 , C07K14/255 , C07K14/26 , C07K14/265 , C07K14/285 , C07K14/295 , C07K14/30 , C07K14/31 , C07K14/315 , C07K14/3156 , C07K14/32 , C07K14/33 , C07K14/34 , C07K14/35 , C07K14/355 , C07K14/37 , C07K14/38 , C07K14/40
摘要： The sequences of antisense nucleic acids which inhibit the proliferation of prokaryotes are disclosed. Cell-based assays which employ the antisense nucleic acids to identify and develop antibiotics are also disclosed. The antisense nucleic acids can also be used to identify proteins required for proliferation, express these proteins or portions thereof, obtain antibodies capable of specifically binding to the expressed proteins, and to use those expressed proteins as a screen to isolate candidate molecules for rational drug discovery programs. The nucleic acids can also be used to screen for homologous nucleic acids that are required for proliferation in cells other than Staphylococcus aureus, Salmonella typhimurium, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The nucleic acids of the present invention can also be used in various assay systems to screen for proliferation required genes in other organisms.
摘要翻译：公开了抑制原核生物增殖的反义核酸序列。还公开了使用反义核酸鉴定和开发抗生素的基于细胞的测定法。反义核酸也可以用于鉴定增殖所需的蛋白质，表达这些蛋白质或其部分，获得能够特异性结合表达的蛋白质的抗体，并使用那些表达的蛋白质作为筛选来分离用于合理药物发现的候选分子程式。核酸也可用于筛选除金黄色葡萄球菌，鼠伤寒沙门氏菌，肺炎克雷伯菌和铜绿假单胞菌以外的细胞中增殖所需的同源核酸。本发明的核酸还可以用于各种测定系统以筛选其它生物体中所需的增殖基因。

59. 发明申请

WO0170955A3 IDENTIFICATION OF ESSENTIAL GENES IN PROKARYOTES 审中-公开
标题翻译：鉴定基因组中的基因
公开(公告)号：WO0170955A3
公开(公告)日：2002-08-01
申请号：PCT/US0109180
申请日：2001-03-21
申请人： ELITRA PHARMACEUTICALS INC , HASELBECK ROBERT , OHLSEN KARI L , ZYSKIND JUDITH W , WALL DANIEL , TRAWICK JOHN D , CARR GRANT J , YAMAMOTO ROBERT T , XU H HOWARD
发明人： HASELBECK ROBERT , OHLSEN KARI L , ZYSKIND JUDITH W , WALL DANIEL , TRAWICK JOHN D , CARR GRANT J , YAMAMOTO ROBERT T , XU H HOWARD
IPC分类号： G01N33/50 , A61K31/7088 , A61K45/00 , A61K48/00 , A61P31/04 , A61P43/00 , C07K14/21 , C07K14/255 , C07K14/26 , C07K14/31 , C07K14/315 , C07K16/12 , C12N1/20 , C12N1/21 , C12N15/09 , C12N15/10 , C12N15/31 , C12P21/02 , C12Q1/06 , C12Q1/68 , G01N33/15 , C12N15/11 , C07K14/195
CPC分类号： C12N15/1079 , A61K31/7088 , A61K48/00 , C07K14/21 , C07K14/255 , C07K14/26 , C07K14/31 , C07K14/315 , C07K16/12 , C12N15/1034
摘要： The sequences of antisense nucleic acids which inhibit the proliferation of prokaryotes are disclosed. Cell-based assays which employ the antisense nucleic acids to identify and develop antibiotics are also disclosed. The antisense nucleic acids can also be used to identify proteins required for proliferation, express these proteins or portions thereof, obtain antibodies capable of specifically binding to the expressed proteins, and to use those expressed proteins as a screen to isolate candidate molecules for rational drug discovery programs. The nucleic acids can also be used to screen for homologous nucleic acids that are required for proliferation in cells other than Staphylococcus aureus, Salmonella typhimurium, Klebsiella pneumoniae, and Pseudomonas aeruginosa . The nucleic acids of the present invention can also be used in various assay systems to screen for proliferation required genes in other organisms.
摘要翻译：公开了抑制原核生物增殖的反义核酸序列。还公开了使用反义核酸鉴定和开发抗生素的基于细胞的测定法。反义核酸也可以用于鉴定增殖所需的蛋白质，表达这些蛋白质或其部分，获得能够特异性结合表达的蛋白质的抗体，并使用那些表达的蛋白质作为筛选来分离用于合理药物发现的候选分子程式。核酸还可用于筛选除金黄色葡萄球菌，鼠伤寒沙门氏菌，肺炎克雷伯杆菌和绿脓假单胞菌以外的细胞中增殖所需的同源核酸。本发明的核酸还可以用于各种测定系统以筛选其它生物体中所需的增殖基因。

60. 发明申请

WO01040280A2 COMPOSITION AND METHOD FOR TREATING A MICROBIAL INFECTION 审中-公开
标题翻译：用于治疗微生物感染的组合物和方法
公开(公告)号：WO01040280A2
公开(公告)日：2001-06-07
申请号：PCT/US2000/042381
申请日：2000-11-29
IPC分类号： A61K39/00 , C07K14/255 , C12N15/31 , C07K14/195
CPC分类号： C07K14/255 , A61K39/00 , A61K2039/505 , C07K2319/00
摘要： The invention provides polypeptides derived from regions of flagellin polypeptides than can be used to generate an immune response to gram-negative bacteria. Also provided are antibodies to the flagellin polypeptides and methods of treating a gram negative bacterial infection using the polypeptides or the antibodies.
摘要翻译：本发明提供衍生自鞭毛蛋白多肽区域的多肽，其可用于产生对革兰氏阴性细菌的免疫应答。还提供了对鞭毛蛋白多肽的抗体以及使用多肽或抗体治疗革兰氏阴性细菌感染的方法。

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