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31. 发明申请

WO2017019125A1 A PROTEIN RICH FOOD INGREDIENT FROM BIOMASS AND METHODS OF PRODUCTION 审中-公开
标题翻译：蛋白质丰富的食物成分从生物量和生产方法
公开(公告)号：WO2017019125A1
公开(公告)日：2017-02-02
申请号：PCT/US2016/014725
申请日：2016-01-25
申请人： SYNTHETIC GENOMICS, INC.
发明人： RUTT, George C. , FLATT, James H. , DOMAILLE, Peter , TOLEDO, Gerardo V.
IPC分类号： A23J3/20 , A23J1/00 , C12N1/12
CPC分类号： A23J1/009 , A23J1/008 , A23K20/147 , A23L7/135 , A23L33/195 , A23V2002/00 , C07K14/405
摘要： The present invention provides a protein material and food ingredient from a sustainable and stable source. The sustainable and stable source of the food or food ingredient is cellular biomass, for example an algal or microbial biomass. The invention discloses that the cellular biomass can be subjected to a series of steps to derive the protein material and food or food ingredient, which has high nutritional content and has pleasing organoleptic properties.
摘要翻译：本发明提供了可持续和稳定的来源的蛋白质材料和食品成分。食物或食品成分的可持续和稳定的来源是细胞生物质，例如藻类或微生物生物量。本发明公开了细胞生物质可以进行一系列步骤以得到具有高营养含量且具有令人愉悦的感官特性的蛋白质材料和食品或食品成分。

32. 发明申请

WO2016140925A1 REGULATORY ELEMENTS FROM LABYRINTHULOMYCETES MICROORGANISMS 审中-公开
标题翻译：乳酸菌微生物的调节元素
公开(公告)号：WO2016140925A1
公开(公告)日：2016-09-09
申请号：PCT/US2016/020114
申请日：2016-02-29
申请人： SYNTHETIC GENOMICS, INC.
发明人： CAIAZZA, Nicky, C. , WIN, Maung, Nyan , URANO, Jun
IPC分类号： C12N15/63 , C12N15/80 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/79 , C12N15/63 , C12N15/80
摘要： The present disclosure generally relates to novel polynucleotide molecules for use in regulating gene expression in recombinant cells, such as labyrinthulomycetes cells. The disclosure further relates to nucleic acid constructs, such as vectors and expression cassettes, containing a regulatory element operably linked to a heterologous nucleotide sequence. The disclosure further relates to methods for stably transforming a host cell, such as a labyrinthulomycetes cell with transgenes. Stably transformed recombinant cells, progeny, biomaterials derived therefrom, and methods for preparing and using the same are also provided.
摘要翻译：本公开通常涉及用于调节重组细胞如迷路形细胞核细胞中的基因表达的新型多核苷酸分子。本公开还涉及含有可操作地连接到异源核苷酸序列的调节元件的核酸构建体，例如载体和表达盒。本公开还涉及用转基因稳定转化宿主细胞如迷路形细胞细胞的方法。还提供了稳定转化的重组细胞，后代，从其衍生的生物材料及其制备和使用方法。

33. 发明申请

WO2014093535A8 PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES 审中-公开
标题翻译： PEG介导的核酸分子组装
公开(公告)号：WO2014093535A8
公开(公告)日：2015-07-02
申请号：PCT/US2013074471
申请日：2013-12-11
申请人： SYNTHETIC GENOMICS INC
发明人： URANO JUN , GIBSON DANIEL G , CAIAZZA NICKY C , QI ZHIQING
IPC分类号： C12P19/34 , C12N15/00 , C12Q1/68
CPC分类号： C12P19/34 , C12N15/1027 , C12N15/1031 , C12Q1/6844 , C12Q2521/101 , C12Q2527/101 , C12Q2527/125 , C12Q2527/143 , C12Q2533/107
摘要： The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.
摘要翻译：本发明公开了从一组重叠寡核苷酸组装核酸分子的方法。该方法包括将一组重叠的寡核苷酸与DNA聚合酶，dNTP的混合物和拥挤剂接触以形成组合混合物。在一个实施方案中，拥挤剂是聚乙二醇（PEG）。拥挤剂的存在促进了本发明的核酸组装过程。然后将组合混合物进行多个循环，每个循环包含退火阶段，延伸相和变性阶段，从而组装所需的核酸分子。在一些实施方案中，一个或多个相是时间变化的。

34. 发明申请

WO2013163613A3 COMPOSITIONS AND METHODS FOR MODULATING THE SENSITIVITY OF CELLS TO AHAS INHIBITORS 审中-公开
标题翻译：用于调节细胞对AHAS抑制剂的灵敏度的组合物和方法
公开(公告)号：WO2013163613A3
公开(公告)日：2014-01-30
申请号：PCT/US2013038517
申请日：2013-04-26
申请人： SYNTHETIC GENOMICS INC
发明人： URANO JUN , CHAMPAGNE MICHELE M
IPC分类号： C12N1/13
CPC分类号： C12N15/8274 , C12N9/1022 , C12N15/79 , C12N15/8214 , C12N15/8278 , C12Y202/01006
摘要： Methods and materials useful for modulating the sensitivity of cells to an inhibitor of acetohydroxyacid synthase (AHAS) are disclosed. For example, nucleic acid molecules encoding AHAS large subunits are disclosed as well as methods for using such nucleic acid molecules to transform microbial cells and plant cells, and to confer modulated sensitivity to AHAS -inhibiting compounds onto such cells. Further provided are materials and methods useful for modulating growth, development, activity, and characteristics of host cells and organisms.
摘要翻译：公开了用于调节细胞对乙酰羟酸合成酶（AHAS）抑制剂的敏感性的方法和材料。例如，公开了编码AHAS大亚基的核酸分子以及使用这种核酸分子转化微生物细胞和植物细胞的方法，并赋予对这种细胞上的AHAS抑制化合物的调节的敏感性。还提供了可用于调节宿主细胞和生物体的生长，发育，活性和特征的材料和方法。

35. 发明申请

WO2013170217A3 MICROBIAL FUEL CELL 审中-公开
标题翻译：微型燃料电池
公开(公告)号：WO2013170217A3
公开(公告)日：2014-01-16
申请号：PCT/US2013040655
申请日：2013-05-10
申请人： SYNTHETIC GENOMICS INC
发明人： BRETSCHGER ORIANNA , WANGER GREG
IPC分类号： H01M8/16
CPC分类号： H01M8/16 , C02F3/005 , H01M4/8605 , H01M4/8673 , Y02E60/527
摘要： Disclosed is a high surface area electrode for use in a microbial fuel cell. In one embodiment the high surface area electrode has an electrode backing and villiated extensions attached to the backing. In one embodiment the villiated extensions and/or electrode backing are made of an electroconductive material such as, for example, graphite or graphite fibers. In one embodiment the electrode is an anode and the electrode backing is in the form of a mesh or woven structure. The electrodes offer superior removal of chemical oxygen demand (COD) and are thus useful in the remediation of wastewaters. The invention also provides microbial fuel cells that utilize the electrodes of the invention. In one embodiment the microbial fuel cells utilize an oxygen barrier and do not utilize a cation or anion or proton exchange membrane.
摘要翻译：公开了一种用于微生物燃料电池的高表面积电极。在一个实施例中，高表面积电极具有附着到背衬的电极背衬和凹陷延伸部。在一个实施例中，凹陷延伸部和/或电极背衬由导电材料制成，例如石墨或石墨纤维。在一个实施例中，电极是阳极，电极背衬是网状或编织结构的形式。电极提供优异的化学需氧量（COD），因此有助于废水的修复。本发明还提供利用本发明的电极的微生物燃料电池。在一个实施方案中，微生物燃料电池利用氧气屏障并且不使用阳离子或阴离子或质子交换膜。

36. 发明申请

WO2013188462A3 REGULATORY ELEMENTS AND USES THEREOF 审中-公开
公开(公告)号：WO2013188462A3
公开(公告)日：2013-12-19
申请号：PCT/US2013/045263
申请日：2013-06-11
申请人： SYNTHETIC GENOMICS, INC.
发明人： CHAMPAGNE, Michele, M. , RICHARDSON, Toby, H. , URANO, Jun
IPC分类号： C12N15/09 , C12N15/113 , C07H21/04
摘要： The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in Nannochloropsis and other microalgae.

37. 发明申请

WO2013028563A2 INTEGRATED METHOD FOR HIGH-THROUGHPUT IDENTIFICATION OF NOVEL PESTICIDAL COMPOSITIONS AND USES THEREFOR 审中-公开
标题翻译：用于高通量鉴定新型农药组合物的综合方法及其用途
公开(公告)号：WO2013028563A2
公开(公告)日：2013-02-28
申请号：PCT/US2012/051466
申请日：2012-08-17
申请人： SYNTHETIC GENOMICS, INC. , GRANDLIC, Christopher, J. , RICHARDSON, Toby , KEROVUO, Janne, S. , SCHWARTZ, Ariel
发明人： GRANDLIC, Christopher, J. , RICHARDSON, Toby , KEROVUO, Janne, S. , SCHWARTZ, Ariel
IPC分类号： C12Q1/68
CPC分类号： C12N15/8286 , C12Q1/6876 , C12Q1/689
摘要： Methods to rapidly identify nucleic acid sequences encoding novel biotoxins are provided. Particularly, methods to rapidly sample and screen extrachromosomal genetic content of microorganisms for novel sequences of interest are described. Compositions comprising coding sequences for biotoxins, and polypeptides and uses derived therefrom are provided. Compositions and methods are useful, for example, for conferring pesticidal activity to bacteria, plants, plant cells, tissues, and seeds.
摘要翻译：提供了快速鉴定编码新生物毒素的核酸序列的方法。特别地，描述了快速采样和筛选微生物染色体外遗传含量的新颖感兴趣的方法。提供了包含编码生物毒素序列的组合物，以及由其衍生的多肽和用途。组合物和方法可用于例如赋予细菌，植物，植物细胞，组织和种子的杀虫活性。

38. 发明申请

WO2011109031A8 METHODS FOR CLONING AND MANIPULATING GENOMES 审中-公开
标题翻译：克隆和操纵基因的方法
公开(公告)号：WO2011109031A8
公开(公告)日：2012-09-20
申请号：PCT/US2010035490
申请日：2010-05-19
申请人： SYNTHETIC GENOMICS INC , BENDERS GWYNEDD A , GLASS JOHN I , HUTCHISON CLYDE A , LARTIGUE CAROLE , VASHEE SANJAY , ALGIRE MIKKEL A , SMITH HAMILTON O , MERRYMAN CHARLES E , NOSKOV VLADIMIR N , CHUANG RAY-YUAN , GIBSON DANIEL G , VENTER J CRAIG
发明人： BENDERS GWYNEDD A , GLASS JOHN I , HUTCHISON CLYDE A , LARTIGUE CAROLE , VASHEE SANJAY , ALGIRE MIKKEL A , SMITH HAMILTON O , MERRYMAN CHARLES E , NOSKOV VLADIMIR N , CHUANG RAY-YUAN , GIBSON DANIEL G , VENTER J CRAIG
IPC分类号： C12N15/10 , C12N15/74
CPC分类号： C12N15/1079 , C12N15/1031 , C12N15/66 , C12N15/81
摘要： Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
摘要翻译：本文公开了在异源宿主细胞中克隆合成或半合成供体基因组的组合物和方法。在一个实施方案中，可以在宿主细胞内进一步修饰供体基因组。修饰或未修饰的基因组可以进一步从宿主细胞中分离并转移到受体细胞。本文公开的方法可用于在更容易处理的宿主细胞中改变来自难治性供体细胞的供体基因组。

39. 发明申请

WO2011153467A2 METHODS TO STIMULATE BIOGENIC METHANE PRODUCTION FROM HYDROCARBON-BEARING FORMATIONS 审中-公开
标题翻译：刺激生物降解甲烷生产的方法
公开(公告)号：WO2011153467A2
公开(公告)日：2011-12-08
申请号：PCT/US2011/039120
申请日：2011-06-03
申请人： SYNTHETIC GENOMICS, INC. , CLEMENT, Brian, G. , FERRY, James, G. , UNDERWOOD, Stuart
发明人： CLEMENT, Brian, G. , FERRY, James, G. , UNDERWOOD, Stuart
CPC分类号： C12N1/26 , C09K8/582 , C09K8/70 , C12N1/20 , C12P5/023 , Y02E50/343
摘要： The present invention describes methods of stimulating the biogenic production of methane in hydrocarbon-bearing formations. The present application provides various stimulants which, when contacted with a hydrocarbon deposit in situ or ex situ , induce or enhance coalbed methane production.
摘要翻译：本发明描述了刺激含烃地层中甲烷生物生成的方法。本申请提供了各种兴奋剂，当与原位或非原位的烃沉积物接触时，其诱导或增强煤层气生产。

40. 发明申请

WO2010102257A3 METHODS FOR CLONING AND MANIPULATING GENOMES 审中-公开
标题翻译：克隆和操纵基因的方法
公开(公告)号：WO2010102257A3
公开(公告)日：2011-01-20
申请号：PCT/US2010026434
申请日：2010-03-05
申请人： SYNTHETIC GENOMICS INC , BENDERS GWYNEDD A , GLASS JOHN I , HUTCHISON CLYDE A , LARTIGUE CAROLE , VASHEE SANJAY , ALGIRE MIKKEL A , SMITH HAMILTON O , MERRYMAN CHARLES E , NOSKOV VLADIMIR N , CHUANG RAY-YUAN , GIBSON DANIEL G , VENTER J CRAIG
发明人： BENDERS GWYNEDD A , GLASS JOHN I , HUTCHISON CLYDE A , LARTIGUE CAROLE , VASHEE SANJAY , ALGIRE MIKKEL A , SMITH HAMILTON O , MERRYMAN CHARLES E , NOSKOV VLADIMIR N , CHUANG RAY-YUAN , GIBSON DANIEL G , VENTER J CRAIG
IPC分类号： C12N15/10
CPC分类号： C12N15/905 , C12N15/10 , C12N15/1031 , C12N15/1079 , C12N15/1093 , C12N15/81
摘要： Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
摘要翻译：本文公开了用于在异源宿主细胞中克隆供体基因组的组合物和方法。在一个实施方案中，可以在宿主细胞内进一步修饰供体基因组。修饰或未修饰的基因组可以进一步从宿主细胞中分离并转移到受体细胞。本文公开的方法可用于在更容易处理的宿主细胞中改变来自难治性供体细胞的供体基因组。

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