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31. 发明申请

WO1997013865A1 SEED SHATTERING 审中-公开
公开(公告)号：WO1997013865A1
公开(公告)日：1997-04-17
申请号：PCT/EP1996004313
申请日：1996-10-04
申请人： PLANT GENETIC SYSTEMS, N.V. , ULVSKOV, Peter , CHILD, Robin , VAN ONCKELEN, Henri , PRINSEN, Els , BORKHARDT, Bernhard , SANDER, Lilli , PETERSEN, Morten , BUNDGARD POULSEN, Gert , BOTTERMAN, Johan
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/82
CPC分类号： C12N15/8249 , C12N9/2402 , C12N15/8206 , C12Y302/01015
摘要： A plant is provided which contains at least one dehiscence zone (DZ)-selective chimeric gene incorporated in the nuclear genome of its cells, wherein said DZ-selective chimeric gene comprises the following operably linked DNA fragments: a) a transcribed DNA region encoding: 1) a RNA which, when produced in cells of a particular DZ of the plant, prevents, inhibits or reduces the expression in said DZ cells of an endogenous gene of the plant encoding a cell wall hydrolase, particularly an endopolygalacturonase, or; 2) a protein or polypeptide, which when produced in said DZ cells, kills or disables them or interferes with their normal metabolism, physiology or development; b) a plant expressible promoter which directs expression of said transcribed DNA region at least in said DZ cells, provided that if said transcribed DNA region encodes a protein or polypeptide, or encodes an antisense RNA or ribozyme directed to a sense RNA encoded by an endogenous gene that is expressed in said plant in cells other than said DZ cells, said plant expressible promoter is a DZ-selective promoter, which directs expression of said transcribed region selectively in said DZ cells and wherein said plant is characterized by modified dehiscence properties, preferably delayed dehiscence, when compared to a plant not containing said DZ-selective chimeric gene.
摘要翻译：提供了一种植物，其包含并入其细胞的核基因组中的至少一个开裂区（DZ）选择性嵌合基因，其中所述DZ选择性嵌合基因包含以下可操作地连接的DNA片段：a）编码： 1）当在植物的特定DZ的细胞中产生时，RNA在编码细胞壁水解酶，特别是内聚半乳糖醛酸酶的植物的内源基因中预防，抑制或降低所述DZ细胞中的表达，或 2）蛋白质或多肽，当在所述DZ细胞中产生时，它们杀死或消灭它们或干扰其正常代谢，生理或发育; b）至少在所述DZ细胞中指导所述转录的DNA区域的表达的植物可表达启动子，条件是如果所述转录的DNA区编码蛋白质或多肽，或编码针对由内源性编码的正义RNA的反义RNA或核酶所述植物可表达启动子是DZ选择性启动子，其指导所述转录区域在所述DZ细胞中的表达，并且其中所述植物的特征在于改性开裂特性，优选地，与不含所述DZ选择性嵌合基因的植物相比时延迟裂开。

32. 发明申请

WO1994026913A3 MARKER GENE 审中-公开
公开(公告)号：WO1994026913A3
公开(公告)日：1994-11-24
申请号：PCT/EP1994001560
申请日：1994-05-11
申请人： PLANT GENETIC SYSTEMS, N.V. , CORNELISSEN, Marcus , REYNAERTS, Arlette , GOSSELE, Véronique , VAN AARSSEN, Roel
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/82

33. 发明申请

WO1993009218A1 MODIFIED GENES AND THEIR EXPRESSION IN PLANT CELLS 审中-公开
标题翻译：修饰基因及其在植物细胞中的表达
公开(公告)号：WO1993009218A1
公开(公告)日：1993-05-13
申请号：PCT/EP1992002547
申请日：1992-10-30
申请人： PLANT GENETIC SYSTEMS, N.V. , CORNELISSEN, Marc , SOETAERT, Piet , STAM, Maike , DOCKX, Jan , VAN AARSSEN, Roel
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/82
CPC分类号： C12N15/67 , C07K14/325 , C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： A DNA fragment, preferably encoding all or an insecticidally-effective part of a Bacillus thuringiensis insecticidal crystal protein, is modified by translationally neutral modifications in cryptic promoters and abortive introns.
摘要翻译：通过在隐性启动子和异常内含子中的翻译中性修饰来修饰优选编码苏云金芽孢杆菌杀虫结晶蛋白的全部或杀虫有效部分的DNA片段。

34. 发明申请

WO1992021757A1 NEMATODE-RESPONSIVE PLANT PROMOTERS 审中-公开
标题翻译： NEMATODE-RESPONSIVE工厂促销
公开(公告)号：WO1992021757A1
公开(公告)日：1992-12-10
申请号：PCT/EP1992001214
申请日：1992-05-29
申请人： PLANT GENETIC SYSTEMS, N.V. , VAN DER EYCKEN, Walter , NIEBEL, Andreas , INZE, Dirk , VAN MONTAGU, Marc , DE WAELE, Dirk , PEFEROEN, Marnix
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/29
CPC分类号： C07K14/415 , C12N15/8285 , Y02A40/164
摘要： Nematode-responsive plant promoters, particularly useful in the production of transgenic plants which can produce fixed feeding site cells that become capable of killing, disabling or repelling nematodes or that are themselves killed or rendered unsuitable for nematodes to feed upon when nematodes infect the plants.
摘要翻译：线虫反应性植物启动子，特别可用于生产转基因植物，转基因植物可以产生固定的饲养位点细胞，其能够杀死，灭活或排斥线虫，或者当线虫感染植物时，它们本身被杀死或不适合用于线虫饲养。

35. 发明申请

WO1992013956A1 STAMEN-SPECIFIC PROMOTERS FROM RICE 审中-公开
标题翻译：来自米兰的特色促销者
公开(公告)号：WO1992013956A1
公开(公告)日：1992-08-20
申请号：PCT/EP1992000274
申请日：1992-02-06
申请人： PLANT GENETIC SYSTEMS, N.V. , MICHIELS, Frank , MORIOKA, Sinji , SCHEIRLINCK, Trees , KOMARI, Toshihiko
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8289 , Y10S47/01
摘要： Rice anther-specific promoters which are of particular utility in the production of transgenic male-sterile monocots and plants for restoring their fertility.
摘要翻译：水稻花药特异性启动子，其特别用于生产转基因雄性不育单子叶植物和植物以恢复其生育力。

36. 发明申请

WO1990015139A1 PREVENTION OF Bt RESISTANCE DEVELOPMENT 审中-公开
标题翻译：防电力发展
公开(公告)号：WO1990015139A1
公开(公告)日：1990-12-13
申请号：PCT/EP1990000905
申请日：1990-05-30
申请人： PLANT GENETIC SYSTEMS N.V. , VAN MELLAERT, Herman , BOTTERMAN, Johan , VAN RIE, Jeroen , JOOS, Henk
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N15/32
CPC分类号： A01N65/08 , A01N65/00 , A01N65/12 , A01N65/38 , C07K14/325 , C07K2319/00 , C12N15/8286 , C12R1/075 , Y02A40/162 , A01N2300/00
摘要： Plants made resistant to insects by transforming their nuclear genome with two or more DNA sequences, each encoding a different non-competitively binding B. thuringiensis protoxin or insecticidal part thereof, preferably the toxin thereof.

37. 发明申请

WO1990014098A1 BACTERICIDAL AND/OR BACTERIOSTATIC PEPTIDE ISOLATED FROM COLEOPTERAN INSECTS 审中-公开
标题翻译：杀虫剂和/或细菌性细菌肽从分离物中分离出来
公开(公告)号：WO1990014098A1
公开(公告)日：1990-11-29
申请号：PCT/EP1990000821
申请日：1990-05-16
申请人： PLANT GENETIC SYSTEMS, N.V. , CASTEELS, Peter , JOOS, Henk , MOLLEY, Koen
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： A61K35/64
CPC分类号： A23L3/3472 , A23L3/3571 , A61K38/00 , C07K14/43563
摘要： An antibacterial factor inducible in the haemolymph of a Coleopteran insect by a bacterial infection.
摘要翻译：通过细菌感染在鞘翅目昆虫的血淋巴中诱导的抗菌因子。

38. 发明申请

WO1990002804A1 STRESS-TOLERANT PLANTS 审中-公开
标题翻译：耐应变植物
公开(公告)号：WO1990002804A1
公开(公告)日：1990-03-22
申请号：PCT/EP1989001025
申请日：1989-08-31
申请人： PLANT GENETIC SYSTEMS, N.V. , BOWLER, Chris , INZE, Dirk , VAN CAMP, Wim , ALLIOTTE, Thiery , VAN MONTAGU, Marc , BOTTERMAN, Johan
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/53
CPC分类号： C12N9/0089 , C12N15/8271
摘要： A plant, the nuclear genome of which is transformed with a recombinant DNA sequence encoding a superoxide dismutase which renders the plant stress-resistant. The recombinant DNA sequence also optionally encodes a targeting peptide, fused to the superoxide dismutase, so that the superoxide dismutase is expressed in the cytoplasm of the plant's cells and is subsequently targeted to mitochondria or chloroplasts of the plant's cells or is secreted, via the endoplastic reticulum, from the plant's cells.
摘要翻译：一种植物，其核基因组用编码超氧化物歧化酶的重组DNA序列转化，其使植物胁迫抗性。重组DNA序列还任选地编码与超氧化物歧化酶融合的靶向肽，使得超氧化物歧化酶在植物细胞的细胞质中表达，随后被靶向植物细胞的线粒体或叶绿体，或通过内胚层分泌网状物，来自植物细胞。

39. 发明申请

WO1990002801A2 PLANTS TRANSFORMED WITH A DNA SEQUENCE FROM BACILLUS THURINGIENSIS LETHAL TO LEPIDOPTERA 审中-公开
标题翻译：植物转化与苏云金芽孢杆菌DNA序列致LEPIDOPTERA
公开(公告)号：WO1990002801A2
公开(公告)日：1990-03-22
申请号：PCT/EP1989001036
申请日：1989-09-05
申请人： PLANT GENETIC SYSTEMS, N.V. , BOTTERMAN, Johan , PEFEROEN, Marnix , HOFTE, Herman , JOOS, Henk
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/32
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： A plant cell transformed with all or part of a Bacillus thuringiensis gene coding for either a 130 or 132 kDa protoxin against Lepidoptera, preferably with a part of the gene coding just for the respective 62 or 60 kDa trypsin-digestion product of the protoxin. A plant, regenerated from the transformed cell, is resistant to Lepidoptera.
摘要翻译：本发明涉及编码前毒素100kDa或130或132抗鳞翅目，优选仅用于摘要的编码基因的一部分与苏云金芽孢杆菌基因的全部或部分转化的植物细胞原毒素的胰蛋白酶kDa 62或60。来自转化细胞的再生植物对鳞翅目具有抗性。

40. 发明申请

WO1989003887A1 A PROCESS FOR THE PRODUCTION OF BIOLOGICALLY ACTIVE PEPTIDE VIA THE EXPRESSION OF MODIFIED STORAGE SEED PROTEIN GENES IN TRANSGENIC PLANTS 审中-公开
标题翻译：生物活性肽生产过程通过转基因植物修饰的储存种子蛋白基因的表达
公开(公告)号：WO1989003887A1
公开(公告)日：1989-05-05
申请号：PCT/EP1988000944
申请日：1988-10-20
申请人： PLANT GENETIC SYSTEMS N.V. , VANDEKERCKHOVE, Joël, Stefaan , KREBBERS, Enno , BOTTERMAN, Johan , LEEMANS, Jan
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12P21/02
CPC分类号： C12N15/8242 , C07K14/00 , C07K14/415 , C07K14/60 , C07K14/70 , C07K2319/02 , C12N15/8234 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8257
摘要： The invention pertains to a process for producing a determined polypeptide of interest or repeats thereof in a seed forming plant. It comprises: cultivating plants obtained from regenerated plant cells or from seeds of plants obtained from said regenerated plant cells over one or several generations, whose genetic patrimony, replicable with said plants, comprises a precursor-coding nucleic acid sequence encoding the precursor of a plant storage protein and placed under the control of a seed promoter, said precursor-coding nucleic acid being modified in a non-essential region of its relevant sequence which encodes the mature storage protein or a sub-unit thereof with a nucleic acid insert in appropriate reading phase relationship with the surrounding part of said relevant sequence, said insert including a determined segment encoding a heterologous determined polypeptide of interest or repeats thereof linked to each other and downstream and upstream thereof to the remainder parts of said relevant sequence through codons encoding aminoacid(s) which define selectively cleavable border sites surrounding the peptide of interest in the hybrid storage protein encoded by the so-modified relevant sequence of said precursor-coding nucleic acid; recovering the seeds of the cultivated plants and extracting the hybrid storage proteins contained therein; cleaving out the peptide of interest from said hybrid storage protein, purifying and recovering the peptide of interest. The polypeptide of interest may be a biologically active protein and/or a labeled protein.
摘要翻译：本发明涉及在种子形成植物中产生所确定的感兴趣的多肽或其重复序列的方法。它包括：从一个或几代的再生植物细胞或从所述再生植物细胞获得的植物种子中培养植物，其遗传遗传可与所述植物复制，其包含编码植物前体的前体编码核酸序列储存蛋白并置于种子启动子的控制之下，所述前体编码核酸在其相关序列的非必需区域中被修饰，所述相关序列编码成熟的存储蛋白或其亚单位，并以适当的读数与核酸插入片段与所述相关序列的周围部分的相位关系，所述插入物包含编码异源确定的感兴趣的多肽或其重复的确定的片段，其通过编码氨基酸（s）的密码子彼此连接并连接到所述相关序列的其余部分），其定义了围绕肽的选择性可切割的边界位点对由所述前体编码核酸的经修饰的相关序列编码的杂交储存蛋白的兴趣; 回收栽培植物的种子并提取其中所含的杂种储存蛋白; 从所述杂交储存蛋白中切出感兴趣的肽，纯化并回收目的肽。感兴趣的多肽可以是生物活性蛋白质和/或标记的蛋白质。

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