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21. 发明申请

WO2015038609A1 ISOTHERMAL AMPLIFICATION USING OLIGOCATION-CONJUGATED PRIMER SEQUENCES 审中-公开
标题翻译：使用低聚合引物序列进行同位素放大
公开(公告)号：WO2015038609A1
公开(公告)日：2015-03-19
申请号：PCT/US2014/054944
申请日：2014-09-10
申请人： GENERAL ELECTRIC COMPANY
发明人： HELLER, Ryan Charles , NELSON, John Richard
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12P19/34 , C12Q1/6865 , C12Q2521/301 , C12Q2523/31 , C12Q2525/179 , C12Q2531/119 , C12Q2531/125 , C12Q2531/143
摘要： Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal DNA amplification methods that employ a strand displacing DNA polymerase and polyamine-oligonucleotide conjugate primer are also provided.
摘要翻译：本文提供了使用寡聚寡核苷酸共轭引物扩增靶核酸以产生扩增子的等温核酸扩增的方法和试剂盒。还提供了使用链置换DNA聚合酶和多胺 - 寡核苷酸共轭引物的等温DNA扩增方法。

22. 发明申请

WO2014062754A1 SECURITY SYSTEM AND METHOD OF MARKING AN INVENTORY ITEM AND/OR PERSON IN THE VICINITY 审中-公开
标题翻译：保护系统和标记物品和/或人员的方法
公开(公告)号：WO2014062754A1
公开(公告)日：2014-04-24
申请号：PCT/US2013/065161
申请日：2013-10-16
申请人： APPLIED DNA SCIENCES, INC.
发明人： BERRADA, Abdelkrim , LIANG, Benjamin, MingHwa , JUNG, Lawrence , JENSEN, Kurt
IPC分类号： G08B15/02 , C12Q1/68
CPC分类号： G08B15/02 , C12Q1/68 , C12Q1/6876 , C12Q2523/308 , C12Q2523/31 , C12Q2563/185
摘要： A method of marking an inventory item includes providing an activatable smoke generator and a reservoir for holding a smoke fluid and adapted to provide a flow of smoke fluid to the generator. The reservoir contains a smoke fluid including a carrier nucleic acid having a uniquely identifiable sequence, and upon activation of the smoke generator, marker smoke is generated and targeted to flow over the inventory item. The method further includes activating the smoke generator to produce the marker smoke including the carrier nucleic acid so as to cause the marker smoke to flow over the inventory item and thereby to detectably mark the inventory item with carrier nucleic acid.
摘要翻译：标记库存物品的方法包括提供可激活的烟雾发生器和用于保持烟雾流体并适于向发生器提供烟雾流体的贮存器。储存器包含烟液，其包括具有唯一可识别顺序的载体核酸，并且在激活烟雾发生器时，产生标记烟雾并将其靶向流过库存物品。该方法还包括激活烟雾发生器以产生包括载体核酸的标记烟，以使标记物烟雾流过库存物品，从而用载体核酸可检测地标记库存物品。

23. 发明申请

WO2014022800A1 COMPOSITIONS AND METHODS FOR IMPROVING NANOPORE SEQUENCING 审中-公开
标题翻译：用于改进纳米级序列的组合物和方法
公开(公告)号：WO2014022800A1
公开(公告)日：2014-02-06
申请号：PCT/US2013/053476
申请日：2013-08-02
申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
发明人： GUNDLACH, Jens, H. , LASZLO, Andrew
IPC分类号： C12Q1/68 , C12N15/11 , G06F19/10 , G01N27/26
CPC分类号： C12Q1/6869 , C12Q2523/31 , C12Q2565/631
摘要： The present disclosure provides methods and reagents for improving nanopore- based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.
摘要翻译：本公开提供了用于改进基于纳米孔的聚合物分析的方法和试剂。具体地，本公开提供了一种分析包含聚合物分析物的聚合物的方法，所述聚合物分析物含有具有至少一个带电部分的末端结构域。本公开还提供了增加聚合物分析物和纳米孔之间的相互作用速率的方法，其中聚合物分析物含有具有至少一个带电部分的末端结构域。本公开还提供了与所述方法一起使用的组合物，包括含有带电部分的接合体组合物，例如磷酸盐或硫酸盐基团，并且被配置为连接到聚合物分析物结构域。

24. 发明申请

WO2012109157A3 BIOPROBES AND METHODS OF USE THEREOF 审中-公开
标题翻译：生物学及其使用方法
公开(公告)号：WO2012109157A3
公开(公告)日：2013-11-14
申请号：PCT/US2012024015
申请日：2012-02-06
申请人： UNIV TORONTO , KELLEY SHANA O , FANG ZHICHAO , VASILYEVA ELIZAVETA
发明人： KELLEY SHANA O , FANG ZHICHAO , VASILYEVA ELIZAVETA
IPC分类号： C12Q1/68 , C07H21/04 , G01N33/50
CPC分类号： C12Q1/6816 , C12N2310/3181 , C12Q1/6837 , C12Q1/6886 , G01N27/26 , G01N27/3276 , G01N27/3277 , C12Q2523/31
摘要： Disclosed are biomolecule based bioprobes that exhibit improved water solubility and mono layer-forming properties with substantially little or no aggregation that can appreciably interfere with binding of the bioprobes to a target nucleotide. The bioprobes may be used in conjunction with a suitable reporter system to detect very small quantities of biological markers. The bio-probes comprise a nucleobase sequence capable of hybridizing to a target nucleotide; and at least one charged functional group attached to said nucleobase sequence. Also disclosed are biosensors, and sensing devices that comprise the bio-probe. Further disclosed are suitable electrochemical reporter systems for use with the bioprobes. Methods of use of these devices and probes, including for the detection of target biomarkers, including biomarkers for cancer cells or pathogens, are also included.
摘要翻译：公开了基于生物分子的生物探针，其表现出改善的水溶性和单层形成性质，其基本上很少或没有聚集，其可以明显地干扰生物探针与靶核苷酸的结合。生物探针可以与合适的报道系统结合使用以检测非常少量的生物标志物。生物探针包含能与靶核苷酸杂交的核碱基序列; 和至少一个连接到所述核碱基序列的带电官能团。还公开了生物传感器和包括生物探针的感测装置。进一步公开的是用于生物探针的合适的电化学报告体系。还包括使用这些装置和探针的方法，包括用于检测靶标生物标志物，包括癌细胞或病原体的生物标志物。

25. 发明申请

WO2013116513A2 DIRECT DETECTION OF DISEASE BIOMARKERS IN CLINICAL SPECIMENS USING CATIONIC NANOPARTICLE-BASED ASSAYS & VERSATILE AND GREEN METHODS FORSYNTHESIS OF ANISOTROPIC SILVER NANOSTRUCTURES 审中-公开
标题翻译：使用阳离子纳米颗粒的测定法和多样性和绿色方法直接检测临床样本中的疾病生物标志物用于各种银纳米结构
公开(公告)号：WO2013116513A2
公开(公告)日：2013-08-08
申请号：PCT/US2013024136
申请日：2013-01-31
申请人： AMERICAN UNIVERSITY OF CAIRO AUC , AZZAZY HASSAN MOHAMED EL-SAID , SHAWKY ABDUO SHERIF MOHAMED , EID KAMEL ABDELMENEM MOHAMED , GUIRGIS BASSEM SAMY SHENOUDA
发明人： AZZAZY HASSAN MOHAMED EL-SAID , SHAWKY ABDUO SHERIF MOHAMED , EID KAMEL ABDELMENEM MOHAMED , GUIRGIS BASSEM SAMY SHENOUDA
CPC分类号： A01N59/16 , B22F1/0003 , B22F9/20 , C12Q1/6816 , C12Q1/70 , G01N33/5308 , G01N33/552 , G01N33/553 , G01N33/587 , Y10T436/143333 , C12Q2523/31 , C12Q2563/155 , C12Q2565/628
摘要： A gold nanoparticle-based assay for the detection of a target molecule, such as Hepatitis C Virus (HCV) RNA in serum samples, that uses positively charged gold nanoparticles (AuNPs) in solution based format. The assay has been tested on 74 serum clinical samples suspected of containing HCV RNA, with 48 and 38 positive and negative samples respectively. The developed assay has a specificity and sensitivity of 96.5% and 92.6% respectively. The results obtained were confirmed by Real-Time PCR, and a concordance of 100% for the negative samples and 89% for the positive samples has been obtained between the Real-Time PCR and the developed AuNPs based assay. Also, a purification method for the HCV RNA has been developed using HCV RNA specific probe conjugated to homemade silica nanoparticles. These silica nanoparticles have been synthesized by modified Stober method. This purification method enhanced the specificity of the developed AuNPs assay. The method can detect a target molecule, such as HCV RNA in serum, by employing modified silica nanoparticles to capture the target from a biological sample followed by detection of the captured target molecule using positively charged AuNPs. The assay is simple, cheap, sensitive and specific. Another aspect of the invention is anisotropic silver nanoparticles and methods of their use.
摘要翻译：基于金纳米粒子的测定法，用于检测目标分子，如血清样品中的丙型肝炎病毒（HCV）RNA，其以基于溶液的形式使用带正电荷的金纳米颗粒（AuNP）。已经对74个怀疑含有HCV RNA的血清临床样品进行了测试，分别有48个和38个阳性和阴性样品。开发的测定分别具有96.5％和92.6％的特异性和灵敏度。获得的结果通过Real-Time PCR证实，在实时PCR和基于开发的AuNPs的测定之间已经获得了阴性样品的100％和阳性样品的89％的一致性。此外，已经开发了使用与自制二氧化硅纳米颗粒缀合的HCV RNA特异性探针的HCV RNA的纯化方法。这些二氧化硅纳米粒子已经通过改良的Stober法合成。该纯化方法增强了开发的AuNPs测定的特异性。该方法可以通过使用改性二氧化硅纳米粒子从生物样品中捕获靶标，然后使用带正电荷的AuNP检测捕获的靶分子，来检测目标分子，如血清中的HCV RNA。该检测方法简单，便宜，敏感且具体。本发明的另一方面是各向异性银纳米颗粒及其使用方法。

26. 发明申请

WO2011100669A2 LABLE-FREE MULTIPLEXING BIOASSAYS USING FLUORESCENT CONJUGATED POLYMERS AND BARCODED NANOPARTICLES 审中-公开
标题翻译：使用荧光共聚聚合物和BARCODED NANOPARTICLES的无线多路复用生物多样性
公开(公告)号：WO2011100669A2
公开(公告)日：2011-08-18
申请号：PCT/US2011/024730
申请日：2011-02-14
申请人： NORTH CAROLINA STATE UNIVERSITY OFFICE OF TECHNOLOGY TRANSFER , HE, Lin , ZHENG, Weiming
发明人： HE, Lin , ZHENG, Weiming
IPC分类号： C12Q1/68 , G01N33/52 , G01N33/58
CPC分类号： G01N33/54313 , C12Q1/6816 , C12Q2523/31 , C12Q2523/313 , C12Q2563/137 , C12Q2563/155 , C12Q2565/514
摘要： Label-free, multiplexed DNA assay using fluorescent conjugated polymers as a detection probe to illustrate hybridization on metallic striped nanorods are disclosed. Different DNA capture probes are encoded by the different reflectivity of Au and Ag stripe patterns. The integration of fluorescent conjugated polymers as detection moieties with metallic striped nanorods for multiplexed detection of clinically important cancer marker proteins in an immunoassay format is also provided.
摘要翻译：公开了使用荧光共轭聚合物作为检测探针的无标记多重DNA测定来说明金属条纹纳米棒上的杂交。不同的DNA捕获探针由Au和Ag条纹图案的不同反射率编码。还提供了荧光共轭聚合物作为检测部分与金属条状纳米棒的整合，用于以免疫测定方式多重检测临床上重要的癌症标志物蛋白质。

27. 发明申请

WO2010034018A2 MSP NANOPORES AND RELATED METHODS 审中-公开
标题翻译： MSP纳米孔和相关方法
公开(公告)号：WO2010034018A2
公开(公告)日：2010-03-25
申请号：PCT/US2009/057915
申请日：2009-09-22
申请人： UNIVERSITY OF WASHINGTON , THE UAB RESEARCH FOUNDATION , GUNDLACH, Jens, H. , NIEDERWEIS, Michael , BUTLER, Thomas, Z. , PAVLENOK, Mikhail , TROLL, Mark, A.
发明人： GUNDLACH, Jens, H. , NIEDERWEIS, Michael , BUTLER, Thomas, Z. , PAVLENOK, Mikhail , TROLL, Mark, A.
IPC分类号： G01N33/68 , C07K14/35 , C12N15/31 , C12N15/63 , C12R1/32
CPC分类号： C12Q1/6869 , A61K39/00 , A61K39/04 , C07K14/35 , C12N13/00 , G01N24/00 , G01N27/44704 , G01N27/44791 , G01N33/48721 , G01N33/6872 , C12Q2523/31 , C12Q2565/631
摘要： Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.
摘要翻译：本文提供了耻垢分枝杆菌孔蛋白纳米孔，包含这些纳米孔的系统，以及使用和制造这些纳米孔的方法。这种纳米孔可以是野生型MspA孔蛋白，突变MspA孔蛋白，野生型MspA旁系同源孔蛋白，野生型MspA同源孔蛋白，突变MspA旁系同源孔蛋白，突变MspA同源孔蛋白或单链Msp孔蛋白。还提供了能够诱导Msp孔蛋白表达的细菌菌株。

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