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21. 发明申请

WO2010068953A2 GREEN PROCESS AND COMPOSITIONS FOR PRODUCING POLY(5HV) AND 5 CARBON CHEMICALS 审中-公开
标题翻译：生产聚（5HV）和5种碳化合物的绿色方法和组合物
公开(公告)号：WO2010068953A2
公开(公告)日：2010-06-17
申请号：PCT/US2009/067943
申请日：2009-12-14
申请人： METABOLIX INC. , FARMER, William R. , BICKMEIER, Jeff , LU, Chenfeng , CHANG, Dong-Eun , SKRALY, Frank , RAMSEIER, Thomas Martin
发明人： FARMER, William R. , BICKMEIER, Jeff , LU, Chenfeng , CHANG, Dong-Eun , SKRALY, Frank , RAMSEIER, Thomas Martin
IPC分类号： C12N1/21 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/80 , C12N9/88 , C12N15/52 , C12P7/04 , C12P7/18 , C12P7/42 , C12P7/44 , C12P7/62
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/0069 , C12N9/10 , C12N9/1029 , C12N9/1096 , C12N9/80 , C12N9/88 , C12N9/93 , C12P7/04 , C12P7/18 , C12P7/42 , C12P7/44 , C12P7/625
摘要： Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminoρentanoate (5AP), 5 -hydroxy valerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is E. coli . The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate).
摘要翻译：提供了用于生产聚羟基链烷酸酯的重组宿主以及由可再生碳基质生产聚羟基链烷酸酯的方法。还提供了产生5种碳化合物的某些重组宿主，如5-氨基戊酸酯（5AP），5-羟基戊酸酯（5HV），戊二酸酯和1,5戊二醇（PDO）。一个实施方案提供了表达编码选自聚羟基链烷酸酯合酶和5-羟基戊酸-CoA（5HV-CoA）转移酶的异源酶的基因的重组宿主，其中宿主产生含有5-羟基戊酸酯的聚合物。优选地，宿主既表达聚羟基链烷酸酯合酶又表达5HV-CoA转移酶。宿主可以是原核生物或真核生物。优选的原核宿主是E. 大肠杆菌的。由重组宿主产生的聚合物可以是5-羟基戊酸酯的均聚物或共聚物。优选的共聚物是聚（3-羟基丁酸酯 - 共-5-羟基戊酸酯）。

22. 发明申请

WO2004024876A3 POLYHYDROXYALKANOATE PRODUCTION BY COENZYME A-DEPENDENT ALDEHYDE DEHYDROGENASE PATHWAYS 审中-公开
标题翻译：聚苯乙烯生产聚苯乙烯依赖性醛酸脱氢酶途径
公开(公告)号：WO2004024876A3
公开(公告)日：2006-05-26
申请号：PCT/US0328842
申请日：2003-09-12
申请人： METABOLIX INC
发明人： SKRALY FRANK A
IPC分类号： C12N1/21 , C12N9/02 , C12N15/52 , C12P7/62 , C07H21/04 , C12N1/20
CPC分类号： C12N9/0008 , C12N15/52 , C12P7/625
摘要： Organisms are provided containing genes encoding one or more enzymes, Coenzyme-A-dependent aldehyde dehydrogenase, acyl-CoA transferase, acyl-CoA synthetase, ß-ketothiolase, acetoacetyl-CoA reductase and/or PHA synthase. In some cases one or more of these genes are native to the host organism and the remainder are heterologous genes provided by genetic engineering. These organisms produce poly (3-hydroxyalkanoate) homopolymers or co-polymers comprising 3-hydroxalkanoate monomers other than 3-hydroxybutryrate wherein these 3-hydroxyalkanoate units are derived from the enzyme-catalyzed conversion of alcohols to 3-hydroxyacyl-CoA monomers, where at least one step in the conversion pathway involves a Co-enzyme A-dependent aldehyde dehydrogenase activity. The PHA polymers are readily recovered and industrially useful as polymers for articles such as films, latexes, coatings, adhesives, fibers, binders, resins, and medical devices.
摘要翻译：提供了包含编码一种或多种酶，辅酶A依赖性醛脱氢酶，酰基辅酶A转移酶，酰基辅酶A合成酶，β-酮硫解酶，乙酰乙酰辅酶A还原酶和/或PHA合成酶的基因的生物体。在某些情况下，这些基因中的一个或多个对于宿主生物是天然的，其余的是通过遗传工程提供的异源基因。这些生物体产生聚（3-羟基链烷酸酯）均聚物或包含除3-羟基丁酸酯之外的3-羟基链烷酸酯单体的共聚物，其中这些3-羟基链烷酸酯单元衍生自醇到3-羟基酰基-CoA单体的酶催化转化，其中转化途径中的至少一步涉及辅酶A依赖性醛脱氢酶活性。 PHA聚合物容易回收，在工业上可用作薄膜，胶乳，涂料，粘合剂，纤维，粘合剂，树脂和医疗装置等物品的聚合物。

23. 发明申请

WO0240690A2 PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM FATTY ACID BIOSYNTHETIC PATHWAYS 审中-公开
标题翻译：从脂肪酸生产途径生产中链长度聚羟基烷酸
公开(公告)号：WO0240690A2
公开(公告)日：2002-05-23
申请号：PCT/US0143686
申请日：2001-11-16
申请人： METABOLIX INC
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12Q1/02 , C12Q1/25 , C12Q1/44 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.
摘要翻译：已经开发了使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径生产聚羟基链烷酸酯（PHA）的方法。使用具有3-羟基酰基ACP硫酯酶的催化活性的酶，非天然细菌PHA生产者和植物中的脂肪酸生物合成途径的PHA生产的方法，具有对于中链长度3-羟基脂肪酸具有底物特异性的酰基辅酶A合成酶，和中链长度PHA合酶。酰基辅酶A合成酶活性可以通过宿主生物体的内源酰基辅酶A合成酶提供，当被充分表达时，或者宿主生物的活性可以通过重组酰基辅酶A合成酶基因的表达补充。描述了用于生物量作物的叶绿体，细胞溶质和过氧化物酶体以及油料种子作物的质体，细胞溶质和过氧化物酶体的基于植物的PHA生产的新策略。

24. 发明申请

WO0234704A2 ALKANOIC ACID ESTER MONOMER COMPOSITIONS AND METHODS OF MAKING SAME 审中-公开
标题翻译：碱性酸酯单体组合物及其制备方法
公开(公告)号：WO0234704A2
公开(公告)日：2002-05-02
申请号：PCT/US0145512
申请日：2001-10-26
申请人： METABOLIX INC
发明人： WHITEHOUSE ROBERT S , ZHONG LUHUA , DAUGHTRY SEAN
IPC分类号： C07C69/675 , C07C67/03 , B01F17/00 , C07C69/708 , C07C69/712 , C07C69/732 , C07C69/734 , C07C69/736 , C08C1/00 , C08C1/065 , C08C1/07 , C08K5/101 , C08K5/103
CPC分类号： C07C69/675
摘要： An alkanoic acid ester monomer is provided having a number average molecular weight of about 1000 or less. The monomer may preferably have a formula selected from: HOCHR(CH2)yCOO A OH (I), H{OCHR(CH2)yCO}xO A OH (II), HOCHR(CH2)yCOO A OOC (CH2)yCHROH (III), H{OCHR(CH2)yCO}xOAO{OC(CH2)yCHRO}xH (IV), {HOCHR)CH2)yCO}zB (V), [H{OCHR(CH2)yCO}x]zB (VI), or T-[-OCR R (CR R )nCO-]p-Q (VII), wherein each R, which can be the same or different, is independently selected from hydrogen, a saturated alkyl group having from about 1 to about 16 carbon atoms, an unsaturated alkyl group having from about 2 to about 16 carbon atoms, or mixtures thereof; A is (CH2)m or (CH2CHR'O)m, where m is from about 1 to about 25 and R' is hydrogen or methyl; x is from about 2 to about 25; y is from 0 to about 3; z is from about 1 to about 5; B is selected from: trimethyl propane when z is 1, 2, 3 or a mixture of 1, 2, and/or 3, glycerol when z is 1, 2, 3 or a mixture of 1, 2, and/or 3, triethanolamine when z is 1, 2, 3 or a mixture of 1, 2, and/or 3, or sucrose when z is 1 to p where p is the number of free hydroxyl groups or derivatives present in said compound; R1, R2, R3, and R4, which are the same as each other or different from one another, are each independently selected from saturated and unsaturated hydrocarbon radicals, halo- and hydroxy-substituted radicals, hydroxy radicals, halogen radicals, nitrogen-substituted radicals, oxygen-substituted radicals, or hydrogen atoms; n is from 0 to about 50, preferably from 0 to about 5; p is from 0 to about 25; Q is selected from: a hydroxy radical or an OR" radical wherein R" is a substituted or unsubstituted alkyl, aryl, alkaryl, or aralkyl radical containing from about 1 to about 20 carbon atoms, and is preferably substituted; OAOH; OAOOC(CH2)yCHROH; OAO{OC(CH2)yCHRO}xH; or B; and T is selected from hydrogen, an alkyl, aryl, alkaryl, or aralkyl group containing from 1 to about 20 carbon atoms, or an R"'COO carboxylate group wherein R"' is an aliphatic or aromatic hydrocarbon radical containing from about 1 to about 20 carbon atoms. Coalescing agents, plasticizers, and green solvents including the monomer are also provided as are methods of making the monomer. In addition, methods of coalescing components, plasticizing compositions, and dissolving solutes using the monomer are also provided.
摘要翻译：提供数均分子量为约1000或更小的链烷酸酯单体。单体可以优选具有选自：HOCHR（CH 2）y COO A OH（I），H（OCHR（CH 2）y CO）x O A OH（II），HOCHR（CH 2）y COO A OOC（CH 2）y CHROH（III），H（OCHR（CH 2）y CO）x OO（OC（CH 2）y CHRO）x H（IV），{HOCHR）CH 2）y CO} z B（V），[H {OCHR（CH 2）y CO} x] 或T - [ - OCR 1 R 2（CR 3 R 4）n CO-] pQ（VII），其中每个R可以相同或不同，独立地选自氢，具有约1至约16个碳原子的饱和烷基，具有约2至约16个碳原子的不饱和烷基，或其混合物; A为（CH 2）m或（CH 2 CHR'O）m，其中m为约1至约25，R'为氢或甲基; x为约2至约25; y为0〜3; z为约1至约5; 当Z为1,2,3或1,2,3或2的混合物时，当z为1,2,3或1,3或2的混合物时，B选自三甲基丙烷：甘油，当z是1,2,3时，当z为1至p时，z为1,2或3或蔗糖的混合物为三乙醇胺，其中p为存在于所述化合物中的游离羟基或衍生物的数目; R 1，R 2，R 3和R 4彼此相同或不同，各自独立地选自饱和和不饱和的烃基，卤代和羟基取代的基团，羟基，卤素基团，氮取代的自由基，氧取代的基团或氢原子; n为0至约50，优选0至约5; p为0至约25; Q选自：羟基或OR“基团，其中R”是含有约1至约20个碳原子的取代或未取代的烷基，芳基，烷芳基或芳烷基，并且优选被取代; OAOH; OAOOC（CH 2）yCHROH; OAO {OC（CH 2）yCHRO} XH; 或B; 并且T选自含有1至约20个碳原子的氢，烷基，芳基，烷芳基或芳烷基，或R“COO羧酸酯基团，其中R”'是含有约1至约20个碳原子的脂族或芳族烃基约20个碳原子。聚合剂，增塑剂和包括单体在内的绿色溶剂也是制备单体的方法。此外，还提供了使用单体聚结组分，增塑组合物和溶解溶质的方法。

25. 发明申请

WO0159091A3 MULTI-GENE EXPRESSION CONSTRUCTS CONTAINING MODIFIED INTEINS 审中-公开
标题翻译：含有改性浓度的多基因表达构建体
公开(公告)号：WO0159091A3
公开(公告)日：2001-12-20
申请号：PCT/US0104254
申请日：2001-02-09
申请人： METABOLIX INC
发明人： SNELL KRISTI D
IPC分类号： C12N15/82 , C12P7/62 , C12N15/63
CPC分类号： C12N15/8216 , C12P7/625
摘要： Methods and constructs for the introduction of multiple genes into plants using a single transformation event are described. Constructs contain a single 5' promoter operably linked to DNA encoding a modified intein splicing unit. The splicing unit is expressed as a polyprotein and consists of a first protein fused to an intein fused to a second protein. The splicing unit has been engineered to promote excision of all non-essential components in the polyprotein but prevent the ligation reactions normally associated with protein splicing. Additional genetic elements encoding inteins and additional proteins can be fused in frame to the 5'-terminus of the coding regions for the second protein to form a construct for expression of more than two proteins. A single 3' termination sequence, such as a polyadenylation sequence when the construct is to be expressed in eucaryotic cells, follows the last coding sequence. These methods and constructs are particularly useful for creating plants with stacked input traits, illustrated by glyphosate tolerant plants producing BT toxin, and/or value added products, illustrated by the production of polyhydroxyalkanoates in plants.
摘要翻译：描述了使用单一转化事件将多个基因引入植物的方法和构建体。构建体包含与编码修饰的内含肽剪接单元的DNA可操作地连接的单个5'启动子。剪接单元表示为多聚蛋白，由融合至第二蛋白质的内含肽融合的第一个蛋白质组成。剪接单元已被设计用于促进多蛋白中所有非必需组分的切除，但是防止通常与蛋白质剪接相关的连接反应。编码内含肽和其他蛋白质的其他遗传元件可以与第二个蛋白质的编码区的5'端融合，形成表达两种以上蛋白质的构建体。当构建体在真核细胞中表达时，单个3'终止序列，例如聚腺苷酸化序列，遵循最后的编码序列。这些方法和结构对于产生具有堆叠输入性状的植物特别有用，由产生BT毒素的草甘膦耐受植物和/或增值产物说明，其通过在植物中生产聚羟基链烷酸酯来说明。

26. 发明申请

WO0006747A3 ENZYMES FOR BIOPOLYMER PRODUCTION 审中-公开
标题翻译：用于生物聚合物生产的酶
公开(公告)号：WO0006747A3
公开(公告)日：2000-03-23
申请号：PCT/US9917452
申请日：1999-07-30
申请人： METABOLIX INC
发明人： PEOPLES OLIVER P , MADISON LARA , HUISMAN GJALT W
IPC分类号： C12N15/09 , C07K5/062 , C07K19/00 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/88 , C12N9/98 , C12N15/53 , C12N15/62 , C12N15/82 , C12P7/62 , C12R1/19 , C12N15/54
CPC分类号： C12N9/0008 , C07K2319/00 , C12N9/10 , C12N9/1025 , C12N9/1029 , C12N9/88 , C12N15/62 , C12N15/82 , C12N15/8243 , C12P7/625
摘要： In order to optimize the flux or flow of carbon intermediates from normal cellular metabolism into PHAs it is desirable to optimize the expression of the enzymes of the PHA biosynthetic pathway. Gene fusions are genetic constructs where two open reading frames have been fused into one and encode hybrid proteins and in some cases bifunctional hybrid enzymes. Linkers may be added to spatially separate the two domains of the hybrid protein. In the case of enzymes which catalyse successive reactions in a pathway, the fusion of two genes results in bringing two enzymatic activities into close proximity to each other. When the product of the first reaction is a substrate for the second one, this new configuration of active sites may result in a faster transfer of the product of the first reaction to the second active site with a potential for increasing the flux through the pathway.
摘要翻译：为了优化从正常细胞代谢到PHA中的碳中间体的通量或流量，期望优化PHA生物合成途径的酶的表达。基因融合体是遗传构建体，其中两个开放阅读框融合成一个并编码杂合蛋白质，在一些情况下编码双功能杂交酶。可以添加接头以空间分离杂交蛋白的两个结构域。在催化途径中连续反应的酶的情况下，两种基因的融合导致两种酶活性彼此靠近。当第一反应的产物是第二反应的产物时，活性部位的这种新构型可导致第一反应产物更快地转移到第二活性位点，从而增加通过通道的通量。

27. 发明申请

WO9851812A3 POLYHYDROXYALKANOATES FOR IN VIVO APPLICATIONS 审中-公开
标题翻译：用于生殖器官应用的聚羟基烷酸酯
公开(公告)号：WO9851812A3
公开(公告)日：1999-02-11
申请号：PCT/US9809834
申请日：1998-05-12
申请人： METABOLIX INC
发明人： WILLIAMS SIMON F , MARTIN DAVID P , GERNGROSS TILLMAN , HOROWITZ DANIEL M
IPC分类号： A61K47/48 , A61L15/26 , A61L17/10 , A61L27/18 , A61L27/34 , A61L29/06 , A61L31/06 , C08G63/06 , C08G63/685 , C08G63/688 , C08G63/91 , C12N5/00 , C12P7/62 , C12P7/00
CPC分类号： C12P7/625 , A61B17/06166 , A61B42/00 , A61K47/593 , A61K47/6957 , A61L15/26 , A61L17/10 , A61L27/18 , A61L27/34 , A61L29/06 , A61L31/06 , C08G63/06 , C08G63/6852 , C08G63/6882 , C08G63/912 , C12N5/0068 , C12N2533/30 , Y10S977/775 , Y10T428/139 , C08L67/04
摘要： Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided for use in numerous biomedical applications. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelia system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. PHAs are provided which are suitable for use in in vivo applications such as in tissue coatings, stents, sutures, tubing, bone and other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses. Properties which are selected for include degradability, elasticity, inclusion of functional groups or derivatized groups, which can in turn be used to attach targeting agents, and bioadhesion.

28. 发明申请

WO1996005316A1 OVERPRODUCTION AND PURIFICATION OF SOLUBLE PHA SYNTHASE 审中-公开
标题翻译：可溶性PHA合成酶的超导和纯化
公开(公告)号：WO1996005316A1
公开(公告)日：1996-02-22
申请号：PCT/US1995010235
申请日：1995-08-10
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , METABOLIX, INC.
发明人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , METABOLIX, INC. , PEOPLES, Oliver, P. , GERNGROSS, Tillman, U. , SINSKEY, Anthony, J.
IPC分类号： C12N15/52
CPC分类号： C12N9/93 , C12N9/00 , C12N9/0006 , C12N9/1029 , C12N15/52 , C12P7/625
摘要： Purified, soluble recombinant bacterial long chain and short chain PHA synthases are described, with methods and materials for overexpression and purification.
摘要翻译：描述了纯化的可溶性重组细菌长链和短链PHA合成酶，其具有用于过表达和纯化的方法和材料。

29. 发明申请

WO2014194220A1 RECYCLATE BLENDS 审中-公开
标题翻译：回收混合物
公开(公告)号：WO2014194220A1
公开(公告)日：2014-12-04
申请号：PCT/US2014/040278
申请日：2014-05-30
申请人： METABOLIX, INC.
发明人： KANN, Yelena , BOUDREAU, David
IPC分类号： C08J11/04 , C08L27/06 , C08L101/00
CPC分类号： C08L67/04 , C08J11/04 , C08J2327/06 , C08J2467/04 , C08L27/06 , C08L101/00 , Y02P20/143 , Y02W30/70
摘要： Compositions comprising recyclate blends of polymer recyclate and polyester additives are described. In certain embodiments, the polyester additive is a PHA such as a poly-3-hydroxybutyrate-co-4-hydroxybutyrate copolymer having a weight percent 4-hydroxybutyrate of 8.5-45%. In other embodiments the PHA is mixed with the recyclate polymer at concentrations of 10-50% by weight of the recyclate blend. Methods of making the compositions of the invention are also described. The invention also includes articles made from the recyclate blends.
摘要翻译：描述了包含聚合物再循环和聚酯添加剂的再循环共混物的组合物。在某些实施方案中，聚酯添加剂是具有8.5-45％重量百分比的4-羟基丁酸酯的PHA，例如聚-3-羟基丁酸酯 - 共-4-羟基丁酸酯共聚物。在其他实施方案中，PHA与回收循环聚合物以回收共混物的10-50重量％的浓度混合。还描述了制备本发明组合物的方法。本发明还包括由回收混合物制成的制品。

30. 发明申请

WO2014100289A1 TRANSCRIPTIONAL REGULATION FOR IMPROVED PLANT PRODUCTIVITY 审中-公开
标题翻译：改良植物生产力的转录规定
公开(公告)号：WO2014100289A1
公开(公告)日：2014-06-26
申请号：PCT/US2013/076308
申请日：2013-12-18
申请人： METABOLIX, INC.
发明人： AMBAVARAM, Madana M.R. , SOMLEVA, Mariya
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8205 , C12N15/8207 , C12N15/8223 , C12N15/8225 , C12N15/8226 , C12N15/8241 , C12N15/8245 , C12N15/8251 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： Methods comprising DNA constructs and polynucleotides of functional transcription factors for improving photosynthetic capacity, biomass and/or grain yield and stress tolerance in various crop and model plants, dicots and monocots with the C 3 or C 4 photosynthetic pathways are described herein.
摘要翻译：本文描述了包含用于改善各种作物和模型植物，双歧杆菌和具有C3或C4光合途径的单子叶植物的光合能力，生物量和/或谷粒产量和胁迫耐受性的功能转录因子的DNA构建体和多核苷酸的方法。

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