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    • 13. 发明申请
    • METHOD OF IDENTIFYING PLANT CELLS TRANSFORMED WITH A GENE
    • 鉴定用基因转化的植物细胞的方法
    • WO2004076625A2
    • 2004-09-10
    • PCT/US2004004332
    • 2004-02-13
    • VECTOR TOBACCO LTDCONKLING MARKLI XIACHEAH KHENGXIE JIAHUA
    • CONKLING MARKLI XIACHEAH KHENGXIE JIAHUA
    • A01H5/00C12N20060101C12N5/04C12N5/10C12N9/10C12N15/82C12N15/84C12N
    • C12N15/821C12N9/1077C12N15/8243
    • Several approaches to differentiate transformed plant cells from non-transformed plant cells, in the absence of a selectable marker, are aspects of the invention described herein. It was discovered that transformed plant cells experience detectable morphological changes (e.g., a reduced rate of growth or biomass) that can be readily identified and used to differentiate the transformed cell from the non-transformed cell in the absence of a selectable marker. Embodiments include approaches for differentiating, identifying, selecting, and regenerating plant cells that have been transformed with a gene of interest, with or without a selectable marker by comparing the growth rate or morphological feature of the transformed plant cells with the growth rate or a morphological feature of non-transformed plant cells that are cultured under similar conditions.
    • 在没有选择性标记的情况下区分转化的植物细胞与未转化的植物细胞的几种方法是本文所述的本发明的方面。 已经发现,转化的植物细胞经历可检测的形态学变化(例如降低的生长速率或生物量),其可以容易地鉴定并用于在不存在选择性标记的情况下将转化的细胞与未转化的细胞区分开。 实施方案包括通过将转化的植物细胞的生长速率或形态特征与生长速率或形态学比较来区分,鉴定,选择和再生已经用感兴趣的基因转化的植物细胞的方法,具有或不具有选择性标记 在相似条件下培养的未转化植物细胞的特征。