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11. 发明申请

WO2014150183A1 METHODS FOR EVALUATING INSECT RESISTANCE IN A PLANT 审中-公开
标题翻译：评估植物耐药性的方法
公开(公告)号：WO2014150183A1
公开(公告)日：2014-09-25
申请号：PCT/US2014/022513
申请日：2014-03-10
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , ALT, Jessie, L. , KRISS, Kevin, A. , MORRIS, Thomas , PRESNAIL, James, K. , RYAN-MAHMUTAGIC, Molly
发明人： ALT, Jessie, L. , KRISS, Kevin, A. , MORRIS, Thomas , PRESNAIL, James, K. , RYAN-MAHMUTAGIC, Molly
IPC分类号： A01C1/08 , A01C1/02
CPC分类号： G01N33/0098 , A01H1/04
摘要： Methods are provided for evaluating the insect resistance of a plant of interest. Specifically, methods are provided for high-throughput screening of soybean plants for resistance to kudzu bugs and stink bugs, including the brown marmorated stink bug and the southern green stink bug. In some embodiments, infesting emergent soybean seedlings with second instar stink bug nymphs and maintaining the nymphs in a closed environment with the seedling for only about 7 days allows evaluation of the stink bug resistance of a soybean variety of interest. Also provided are insect resistant plants, particularly plants resistant to kudzu bugs and stink bugs, as well as seeds produced by the resistant plants. Plants disclosed herein can be used to transfer the resistant trait into plant lines of interest.
摘要翻译：提供了评估感兴趣植物的抗虫性的方法。具体来说，提供了大豆植物高通量筛选方法，用于抵抗葛虫和臭臭虫，包括棕色马蹄臭虫和南方绿色臭虫。在一些实施方案中，具有第二龄臭虫若虫的侵袭性大豆幼苗并且在封闭的环境中将幼苗保持在约7天的幼苗中，可以评估感兴趣的大豆品种的臭虫抗性。还提供了抗虫植物，特别是抗葛虫和臭虫的植物，以及由抗性植物产生的种子。本文公开的植物可以用于将抗性性状转移到感兴趣的植物系中。

12. 发明申请

WO2009091862A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 审中-公开
公开(公告)号：WO2009091862A3
公开(公告)日：2009-07-23
申请号：PCT/US2009/031055
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DUPONT DE NEMOURS & COMPANY , HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
IPC分类号： C12N15/82
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

13. 发明申请

WO2007149657A2 ANTIFUNGAL POLYPEPTIDES 审中-公开
标题翻译：抗菌多糖
公开(公告)号：WO2007149657A2
公开(公告)日：2007-12-27
申请号：PCT/US2007068984
申请日：2007-05-15
申请人： PIONEER HI BRED INT , DU PONT , UNIV CALIFORNIA , ALTIER DANIEL J , CRANE VIRGINIA C , GILLIAM JACOB T , HUNTER-CEVERA JENNIE , PRESNAIL JAMES K , SCHEPERS ERIC J , SIMMONS CARL R , TOROK TAMAS , YALPANI NASSER
发明人： ALTIER DANIEL J , CRANE VIRGINIA C , GILLIAM JACOB T , HUNTER-CEVERA JENNIE , PRESNAIL JAMES K , SCHEPERS ERIC J , SIMMONS CARL R , TOROK TAMAS , YALPANI NASSER , ELLANSKAYA I A
CPC分类号： C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。也提供了编码抗病原多肽的核酸。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。

14. 发明申请

WO2007087567A3 ANTIFUNGAL POLYPEPTIDES 审中-公开
公开(公告)号：WO2007087567A3
公开(公告)日：2007-08-02
申请号：PCT/US2007/060982
申请日：2007-01-24
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , ALTIER, Daniel, J. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
发明人： ALTIER, Daniel, J. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
IPC分类号： C12N15/82 , A01H5/00 , C12Q1/68 , C12R1/77
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

15. 发明申请

WO2005118628A3 NUCLEIC ACID MOLECULES ENCODING PLANT CYCLOTIDE POLYPEPTIDES AND METHODS OF USE 审中-公开
标题翻译：编码植物循环多聚核苷酸的核酸分子及其使用方法
公开(公告)号：WO2005118628A3
公开(公告)日：2006-05-04
申请号：PCT/US2005018433
申请日：2005-05-25
申请人： DU PONT , HERRMANN RAFAEL , LU ALBERT LAURENCE , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WAINE CLEMENT W K
发明人： HERRMANN RAFAEL , LU ALBERT LAURENCE , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WAINE CLEMENT W K
IPC分类号： C07K14/415 , A01H1/00 , A61K38/16 , C07H21/04 , C07K7/64 , C07K14/00 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8285 , C07K14/415 , C12N15/8282 , Y02A40/164
摘要： The present invention relates to isolated nucleic acids encoding plant cyclotides. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
摘要翻译：本发明涉及编码植物环肽的分离的核酸。本发明还涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环磷酰胺。

16. 发明申请

WO2006014316A1 ANTIFUNGAL POLYPEPTIDES 审中-公开
标题翻译：抗菌多糖
公开(公告)号：WO2006014316A1
公开(公告)日：2006-02-09
申请号：PCT/US2005/023339
申请日：2005-07-01
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMUORS AND COMPANY , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , ALTIER, Daniel, J., Altier , DAHLBACKA, Glen , HERRMANN, Rafael , HUNTER-CEVERA, Jennie , McCUTCHEN, Billy, Fred , PRESNAIL, James, K. , SCHEPERS, Eric, J. , RICE, Janet, A. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
发明人： ALTIER, Daniel, J., Altier , DAHLBACKA, Glen , HERRMANN, Rafael , HUNTER-CEVERA, Jennie , McCUTCHEN, Billy, Fred , PRESNAIL, James, K. , SCHEPERS, Eric, J. , RICE, Janet, A. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
IPC分类号： C07K14/385 , C07K14/37
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

17. 发明申请

WO2005063996A2 PLANT ACTIVATION OF INSECT TOXIN 审中-公开
标题翻译：昆虫毒素的植物激活
公开(公告)号：WO2005063996A2
公开(公告)日：2005-07-14
申请号：PCT/US2004/042736
申请日：2004-12-20
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , ABAD, André , FLANNAGAN, Ronald, D. , HERRMANN, Rafael , LU, Albert , MCCUTCHEN, Billy, F. , PRESNAIL, James, K. , RICE, Janet, A. , WONG, James, F. , YU, Cao-Guo
发明人： ABAD, André , FLANNAGAN, Ronald, D. , HERRMANN, Rafael , LU, Albert , MCCUTCHEN, Billy, F. , PRESNAIL, James, K. , RICE, Janet, A. , WONG, James, F. , YU, Cao-Guo
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译：提供了用于保护植物免受害虫侵害的组合物和方法。具体而言，提供了编码经修饰以包含至少一个对植物蛋白酶或昆虫肠蛋白酶敏感的蛋白水解活化位点的昆虫原毒素的核酸序列。在蛋白水解活化位点被蛋白酶切割修饰的原毒素产生活性昆虫毒素。提供了使用修饰的昆虫原毒素核酸序列和它们编码的多肽来保护植物免受害虫的方法。本发明的特定实施方案进一步提供了修饰的昆虫原毒素组合物和制剂，表达盒和转化的植物，植物细胞和种子。

18. 发明申请

WO02086072A8 ANTIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：抗微生物多肽及其用途
公开(公告)号：WO02086072A8
公开(公告)日：2002-12-12
申请号：PCT/US0212511
申请日：2002-04-19
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H , DU PONT
发明人： ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N7/01 , C12N15/12 , C12N15/82 , C12N
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

19. 发明申请

WO2009091864A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 审中-公开
标题翻译：抑制淋巴瘤靶向多核苷酸的组合物和方法
公开(公告)号：WO2009091864A3
公开(公告)日：2009-12-10
申请号：PCT/US2009031057
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , C12N15/113 , C12N15/8218 , C12N2310/141 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物如来自草盲蝽属的害虫摄入时，能够降低害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了来自本文公开的特定多肽家族的各种靶多核苷酸，并且还提供了SEQ ID NO：1-21中列出的靶多核苷酸或其活性变体，其中靶害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是草盲蝽（Lygus Hesperus）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。

20. 发明申请

WO2009091860A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE 审中-公开
标题翻译：用于抑制来自家庭蚜虫目标多核苷酸的组合物和方法
公开(公告)号：WO2009091860A3
公开(公告)日：2009-11-05
申请号：PCT/US2009031053
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C07K14/43563 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物例如来自蚜科的害虫摄取时，能够降低害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了特定蛋白质类别的靶多核苷酸以及如SEQ ID NO：1,2,3,4,5,6,7,8,9,10或其活性变体或片段所示的靶多核苷酸，其中a 目标害虫中一个或多个序列表达的减少控制了害虫（即，具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是蚜虫（Aphis）甘氨酸。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。

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