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91. 发明申请

WO2006101983A3 CHEMICALLY INDUCIBLE EXPRESSION OF BIOSYNTHETIC PATHWAYS 审中-公开
公开(公告)号：WO2006101983A3
公开(公告)日：2006-09-28
申请号：PCT/US2006/009531
申请日：2006-03-16
申请人： METABOLIX, INC. , KOURTZ, Lauralynn , PEOPLES, Oliver, P. , SNELL, Kristi, D.
发明人： KOURTZ, Lauralynn , PEOPLES, Oliver, P. , SNELL, Kristi, D.
IPC分类号： C12N15/82 , C12P7/62 , A01H5/00
摘要： Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.

92. 发明申请

WO2006101983A2 CHEMICALLY INDUCIBLE EXPRESSION OF BIOSYNTHETIC PATHWAYS 审中-公开
标题翻译：化学途径的化学诱导表达
公开(公告)号：WO2006101983A2
公开(公告)日：2006-09-28
申请号：PCT/US2006009531
申请日：2006-03-16
申请人： METABOLIX INC , KOURTZ LAURALYNN , PEOPLES OLIVER P , SNELL KRISTI D
发明人： KOURTZ LAURALYNN , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： C12N15/82 , A01H5/00 , C12P7/62
CPC分类号： C12N15/8247 , C12N15/8237 , C12N15/8243
摘要： Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.
摘要翻译：提供了在多酶生物合成途径中引入编码酶的多个基因的方法和构建体。在一个实施方案中，构建体含有两个或更多个酶编码基因，每个基因在诱导型启动子的控制下并且各自具有多聚腺苷酸化信号。构建体用于产生转基因植物，其中当施用化学诱导剂时酶的表达增加，并且产生由转基因编码的一系列酶的生物合成产物。可以使用含有两个或更多个酶编码基因的构建体，所述两个或多个酶编码基因在一个或多个由活化剂分子或由转基因或转基因表达的复合物所激活的启动子的控制下，所述启动子本身处于一个或多个诱导型启动子的控制下，化学品的外部应用。表达活化剂分子或复合物的转基因或转基因可以包含在含有多酶生物合成途径中的酶的多个基因的相同构建体中。或者，表达活化剂分子或复合物的转基因或转基因可以与含有多酶生物合成途径中的酶的多个基因的构建体在不同的构建体上。可以使用非活性形式的组成型启动子来表达活化剂分子，其在施用化学诱导剂之后转化为活性形式。

93. 发明申请

WO2006031492A1 SINGLE SOLVENT POLYMER EXTRACTION METHODS 审中-公开
标题翻译：单溶剂聚合物萃取方法
公开(公告)号：WO2006031492A1
公开(公告)日：2006-03-23
申请号：PCT/US2005/031624
申请日：2005-09-06
申请人： METABOLIX, INC. , ANDERSON, Erik , ANDERSON, Erik , LICATA, John
发明人： ANDERSON, Erik , ANDERSON, Erik , LICATA, John
IPC分类号： C08G63/02
CPC分类号： C08G63/89 , C08G63/06
摘要： This invention relates to single solvent polymer extraction methods.
摘要翻译：本发明涉及单一溶剂聚合物提取方法。

94. 发明申请

WO2004013204A3 POLYMER EXTRACTION METHODS 审中-公开
标题翻译：聚合物萃取方法
公开(公告)号：WO2004013204A3
公开(公告)日：2004-06-03
申请号：PCT/US0323034
申请日：2003-07-23
申请人： METABOLIX INC , WALSEM JOHAN VAN , ZHONG LUHUA , SHIH SIMON S
发明人： WALSEM JOHAN VAN , ZHONG LUHUA , SHIH SIMON S
IPC分类号： C12P7/62 , C08G63/06 , C08G63/89 , C12Q1/68 , G01N33/53
CPC分类号： C08G63/89 , C08G63/06
摘要： The invention features methods of separating a polymer from a biomass containing the polymer. In one aspect, the method includes contacting the biomass with a solvent system to provide a residual biomass and a solution. The solvent system includes a solvent for the polymer and a precipitant for the polymer, and the solution includes the polymer, the solvent for the polymer and the precipitant for the polymer. Applying a centrifugal force to the solution and residual biomass to separate at least some of the solution from the residual biomass. In another aspect, the method includes contacting the biomass with a solvent system to provide a residual biomass and a solution including the polymer and the solvent system; adding a precipitant for the polymer to the solution; and a separating at least some of the solution from the residual biomass after adding the precipitant for the polymer.
摘要翻译：本发明的特征在于从含有聚合物的生物质中分离聚合物的方法。一方面，该方法包括使生物质与溶剂系统接触以提供残留的生物质和溶液。溶剂系统包括聚合物的溶剂和聚合物的沉淀剂，溶液包括聚合物，聚合物的溶剂和聚合物的沉淀剂。将离心力施加到溶液和残余生物质以将至少一些溶液与剩余生物质分离。在另一方面，该方法包括使生物质与溶剂体系接触以提供残余生物质和包括聚合物和溶剂系统的溶液; 向溶液中加入聚合物的沉淀剂; 以及在为聚合物加入沉淀剂之后，从残余生物质中分离出至少一些溶液。

95. 发明申请

WO2004024876A2 POLYHYDROXYALKANOATE PRODUCTION BY COENZYME A-DEPENDENT ALDEHYDE DEHYDROGENASE PATHWAYS 审中-公开
标题翻译：聚苯乙烯生产聚苯乙烯依赖性甲醛脱氢酶途径
公开(公告)号：WO2004024876A2
公开(公告)日：2004-03-25
申请号：PCT/US2003/028842
申请日：2003-09-12
申请人： METABOLIX, INC.
发明人： SKRALY, Frank, A.
IPC分类号： C12N
CPC分类号： C12N9/0008 , C12N15/52 , C12P7/625
摘要： Organisms are provided containing genes encoding one or more enzymes, Coenzyme-A-dependent aldehyde dehydrogenase, acyl-CoA transferase, acyl-CoA synthetase, ß-ketothiolase, acetoacetyl-CoA reductase and/or PHA synthase. In some cases one or more of these genes are native to the host organism and the remainder are heterologous genes provided by genetic engineering. These organisms produce poly (3-hydroxyalkanoate) homopolymers or co-polymers comprising 3-hydroxalkanoate monomers other than 3-hydroxybutryrate wherein these 3-hydroxyalkanoate units are derived from the enzyme-catalyzed conversion of alcohols to 3-hydroxyacyl-CoA monomers, where at least one step in the conversion pathway involves a Co-enzyme A-dependent aldehyde dehydrogenase activity. The PHA polymers are readily recovered and industrially useful as polymers for articles such as films, latexes, coatings, adhesives, fibers, binders, resins, and medical devices.
摘要翻译：提供了包含编码一种或多种酶，辅酶A依赖性醛脱氢酶，酰基辅酶A转移酶，酰基辅酶A合成酶，β-酮硫解酶，乙酰乙酰辅酶A还原酶和/或PHA合成酶的基因的生物体。在某些情况下，这些基因中的一个或多个对宿主生物是天然的，其余的是通过遗传工程提供的异源基因。这些生物体产生聚（3-羟基链烷酸酯）均聚物或包含除3-羟基丁酸酯之外的3-羟基链烷酸酯单体的共聚物，其中这些3-羟基链烷酸酯单元衍生自醇到3-羟基酰基-CoA单体的酶催化转化，其中在转化途径中的至少一个步骤涉及辅酶A依赖性醛脱氢酶活性。 PHA聚合物容易回收并且在工业上可用作用于诸如膜，胶乳，涂料，粘合剂，纤维，粘合剂，树脂和医疗装置的制品的聚合物。

96. 发明申请

WO2004013204A2 POLYMER EXTRACTION METHODS 审中-公开
标题翻译：聚合物萃取方法
公开(公告)号：WO2004013204A2
公开(公告)日：2004-02-12
申请号：PCT/US2003/023034
申请日：2003-07-23
申请人： METABOLIX INC. , WALSEM, Johan, Van , ZHONG, Luhua , SHIH, Simon, S.
发明人： WALSEM, Johan, Van , ZHONG, Luhua , SHIH, Simon, S.
IPC分类号： C08G63/89
CPC分类号： C08G63/89 , C08G63/06
摘要： The invention features methods of separating a polymer from a biomass containing the polymer. In one aspect, the method includes contacting the biomass with a solvent system to provide a residual biomass and a solution. The solvent system includes a solvent for the polymer and a precipitant for the polymer, and the solution includes the polymer, the solvent for the polymer and the precipitant for the polymer. Applying a centrifugal force to the solution from the residual biomass to separate at least some of the solution from the residual biomass. In another aspect, the method includes contacting the biomass with a solvent system to provide a residual biomass and a solution including the polymer and the solvent system; adding a precipitant for the polymer to the solution; and a separating at least some of the solution from the residual biomass after adding the precipitant for the polymer.
摘要翻译：本发明的特征在于从含有聚合物的生物质中分离聚合物的方法。一方面，该方法包括使生物质与溶剂系统接触以提供残留的生物质和溶液。溶剂系统包括聚合物的溶剂和聚合物的沉淀剂，溶液包括聚合物，聚合物的溶剂和聚合物的沉淀剂。将来自残余生物质的离心力施加到溶液中以将至少一些溶液与剩余生物质分离。另一方面，该方法包括使生物质与溶剂体系接触以提供残留的生物质和包括聚合物和溶剂体系的溶液; 向溶液中加入聚合物的沉淀剂; 以及在为聚合物加入沉淀剂之后，从残余生物质中分离出至少一些溶液。

97. 发明申请

WO2003051813A1 METHODS OF MAKING INTERMEDIATES FROM POLYHYDROXYALKANOATES 审中-公开
标题翻译：从聚羟基烷基酯制备中间体的方法
公开(公告)号：WO2003051813A1
公开(公告)日：2003-06-26
申请号：PCT/US2002/040900
申请日：2002-12-18
申请人： METABOLIX, INC. , ZHONG, Luhua , MULLER, Edward, M. , BARBER, James, J. , PUGACH, Joseph , WHITHEHOUSE, Robert, S. , DAUGHTRY, Sean
发明人： ZHONG, Luhua , MULLER, Edward, M. , BARBER, James, J. , PUGACH, Joseph , WHITHEHOUSE, Robert, S. , DAUGHTRY, Sean
IPC分类号： C07C67/02
CPC分类号： C07C51/377 , C07C29/149 , C07C51/09 , C07C67/03 , C07C235/06 , C07C2601/14 , C07C31/207 , C07C31/205 , C07C31/202 , C07C31/20 , C07C57/08 , C07C69/675
摘要： Methods of forming intermediates from PHAa are disclosed.
摘要翻译：公开了从PHAa形成中间体的方法。

98. 发明申请

WO0056376A9 MEDICAL DEVICES AND APPLICATIONS OF POLYHYDROXYALKANOATE POLYMERS 审中-公开
标题翻译：聚羟基烷基酯聚合物的医疗器械及其应用
公开(公告)号：WO0056376A9
公开(公告)日：2002-07-11
申请号：PCT/US0007890
申请日：2000-03-24
申请人： METABOLIX INC
发明人： WILLIAMS SIMON F , MARTIN DAVID P , SKRALY FRANK
IPC分类号： A61B17/58 , A61F2/84 , A61K45/00 , A61L15/64 , A61L17/00 , A61L17/12 , A61L27/00 , A61L27/18 , A61L27/58 , A61L31/06 , A61L31/14 , C08G63/06 , C08K5/00 , C12P7/62
CPC分类号： A61L27/58 , A61F2/07 , A61F2210/0004 , A61K31/715 , A61L17/12 , A61L27/18 , A61L31/06 , A61L31/148 , A61L31/16 , A61L2300/00 , C08G63/06 , C08J9/26 , C08J2201/0444 , C08J2201/0482 , C08J2367/04 , C08K5/0033 , C08L67/04 , C12P7/625 , Y10S606/911
摘要： Devices formed of or including biocompatible polyhydroxylkanoates are provided with controlled degradation rates, preferably less than one year under physiological conditions. Preferred devices include sutures, suture fasteners, meniscus repair devices, rivets, tacks, staples, screws (including interference screws), bone plates and bone plating system, surgical mesh, repair patches, slings, cardiovascular patches, orthopedic pins (including bone filling augmentation material), adhesion barriers, stents, guided tissue repair/regeneration devices, articular cartilage repair devices, nerve guides, tendon repair devices, atrial septal defect repair devices, pericardial patches, bulking and filling agents, vein valves, bone marrow scaffolds, meniscus regeneration devices, ligament and tendon grafts, ocular cell implants, spinal fusion cages, skin substitutes, dural substitutes, bone graft substitutes, bone dowels, wound dressings, and hemostats. The polyhydroxyalkanoates can contain additives, be formed of mixtures of monomers or include pendant groups or modifications in their backbones, or can be chemically modified, all to alter the degradation rates. The polyhydroxyalkanoate compositions also provide favorable mechanical properties, biocompatibility, and degradation times within desirable time frames under physiological conditions.
摘要翻译：由生物相容性聚羟基烷酸酯形成或包括生物相容性聚羟基烷酸酯的设备具有受控的降解速率，优选在生理条件下小于一年。优选的装置包括缝线，缝线紧固件，弯液面修复装置，铆钉，钉，钉，螺钉（包括干涉螺钉），骨板和骨电镀系统，外科手术网，修复贴片，吊索，心血管贴片，矫形针材料），粘附屏障，支架，引导组织修复/再生装置，关节软骨修复装置，神经导管，肌腱修复装置，房间隔缺损修复装置，心包块，填充剂和填充剂，静脉瓣，骨髓支架，半月板再生装置，韧带和腱移植物，眼细胞植入物，脊髓融合笼，皮肤替代物，硬脑膜替代物，骨移植物替代物，骨榫，伤口敷料和止血剂。聚羟基链烷酸酯可以含有由单体的混合物形成的添加剂，或者在其主链中包括侧基或修饰，或者可以进行化学改性，以改变降解速率。聚羟基链烷酸酯组合物还在生理条件下的期望时间范围内提供有利的机械性能，生物相容性和降解时间。

99. 发明申请

WO0216284A3 LOW MOLECULAR WEIGHT POLYHYDROXYALKANOATE MOLDING COMPOSITIONS 审中-公开
标题翻译：低分子量聚羟基脂肪酸酯模塑组合物
公开(公告)号：WO0216284A3
公开(公告)日：2002-05-16
申请号：PCT/US0126061
申请日：2001-08-21
申请人： METABOLIX INC
发明人： ZHONG LUHUA , WHITEHOUSE ROBERT S
IPC分类号： B22F1/00 , C04B35/634 , C08K3/08 , C08K3/34 , C08K3/40 , C08L67/04 , C08K3/00
CPC分类号： C08K3/34 , B22F1/0059 , C04B35/634 , C08K3/08 , C08K3/40 , C08L67/04 , C08L67/00 , C08L2666/02
摘要： Molding compositions including low molecular weight polyhydroxyalkanoates are provided. The use of the low molecular weight polyhydroxyalkanoates as a binder in molding compositions provides improved binder removal in the finished molded product, and offers a wide range of physical properties suitable for use in a variety of processing conditions. The composition preferably includes a powdered material, such as a metal powder, ceramic powder, or blend, admixed with a low molecular weight polyhydroxyalkanoate binder. The compositions are useful in powder processing techniques, such as injection molding, slip casting, tape casting, or extrusion. Methods of forming low molecular weight polyhydroxyalkanoates are also described.
摘要翻译：提供了包含低分子量聚羟基链烷酸酯的模塑组合物。在模塑组合物中使用低分子量聚羟基链烷酸酯作为粘合剂提供了改进的成品模制品中脱粘合剂的改进，并提供适用于各种加工条件的各种物理性能。该组合物优选包括与低分子量聚羟基链烷酸酯粘合剂混合的粉末材料，例如金属粉末，陶瓷粉末或混合物。该组合物可用于粉末加工技术，如注塑，粉浆浇铸，流延成型或挤出。还描述了形成低分子量聚羟基链烷酸酯的方法。

100. 发明申请

WO2002034704A3 HYDROXY-ALKANOIC ACID ESTER MONOMER COMPOSITIONS AND METHODS OF MAKING SAME 审中-公开
公开(公告)号：WO2002034704A3
公开(公告)日：2002-05-02
申请号：PCT/US2001/045512
申请日：2001-10-26
申请人： METABOLIX, INC.
发明人： WHITEHOUSE, Robert, S. , ZHONG, Luhua , DAUGHTRY, Sean
IPC分类号： C07C67/03
摘要： An alkanoic acid ester monomer is provided having a number average molecular weight of about 1000 or less. Coalescing agents, plasticizers, and green solvents including the monomer are also provided as are methods of making the monomer. In addition, methods of coalescing components, plasticizing compositions, and dissolving solutes using the monomer are provided.

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