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1. 发明申请

WO2008105905A2 METHODS AND COMPOSITIONS FOR IMPROVED ENZYME ACTIVITY IN TRANSGENIC PLANTS 审中-公开
标题翻译：提高转基因植物酶活性的方法和组合物
公开(公告)号：WO2008105905A2
公开(公告)日：2008-09-04
申请号：PCT/US2007073988
申请日：2007-07-20
申请人： ATHENIX CORP , KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
发明人： KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
IPC分类号： A01H1/00 , A01H5/00
CPC分类号： C12N15/8275
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译：提供了用于在植物，植物细胞，组织和种子中的广泛生理谱上增加酶活性的组合物和方法。组合物包括植物或植物部分，所述植物或植物部分包含两种或更多种编码多肽的多核苷酸，所述多核苷酸在多核苷酸单独表达时比在更广泛的生理光谱中有活性进一步提供了包含这些多核苷酸分子的载体以及包含该载体的宿主细胞。组合物还包含转化的细菌，植物，植物细胞，组织和种子。另外，提供了用于生产本发明的植物，植物细胞，组织和种子的方法。还提供了增加植物产量的方法和赋予植物对除草剂抗性的方法。

2. 发明申请

WO2007103768A2 METHODS AND COMPOSITIONS FOR IMPROVED ENZYME ACTIVITY IN TRANSGENIC PLANT 审中-公开
标题翻译：在转基因植物中改善酶活性的方法和组合物
公开(公告)号：WO2007103768A2
公开(公告)日：2007-09-13
申请号：PCT/US2007063132
申请日：2007-03-02
申请人： ATHENIX CORP , KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
发明人： KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
IPC分类号： A01H5/00
CPC分类号： C12N15/8261 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译：提供了用于增加植物，植物细胞，组织和种子中广泛生理光谱的酶活性的组合物和方法。组合物包括植物或植物部分，其包含两个或更多个编码与更多生理光谱相关的多肽的多核苷酸，而不是单个表达。还提供了包含这些多核苷酸分子的载体以及包含载体的宿主细胞。组合物还包括转化的细菌，植物，植物细胞，组织和种子。此外，提供了用于制备本发明的植物，植物细胞，组织和种子的方法。还提供了增加植物产量的方法和赋予植物中除草剂抗性的方法。

3. 发明申请

WO2004081185B1 METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES 审中-公开
标题翻译：提高木糖醇降解酶活性的方法
公开(公告)号：WO2004081185B1
公开(公告)日：2004-12-23
申请号：PCT/US2004007086
申请日：2004-03-08
申请人： ATHENIX CORP , BURDETTE JILL , VANDE BERG BRIAN , CARR BRIAN , DUCK NICHOLAS B , CAROZZI NADINE , KOZIEL MICHAEL G , PATEL PARESMA R
发明人： BURDETTE JILL , VANDE BERG BRIAN , CARR BRIAN , DUCK NICHOLAS B , CAROZZI NADINE , KOZIEL MICHAEL G , PATEL PARESMA R
IPC分类号： C12N20060101 , C12N9/04 , C12N9/18 , C12N9/20 , C12P7/10 , C12P19/02 , C12S3/00 , C13K1/02 , D06M10/00
CPC分类号： C12P7/10 , C08H8/00 , C12N9/0006 , C12N9/18 , C12N9/20 , D21C5/005 , D21C9/16 , D21C11/0007 , Y02E50/16
摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.
摘要翻译：提供水解木质纤维素的方法，包括使木质纤维素与至少一种化学处理接触。还提供了用于预处理木质纤维素材料的方法，包括使材料与至少一种化学品接触。还提供了用于从植物材料释放诸如酶，药物或营养品的物质的方法。这些方法比目前的方法更有效，更经济，毒性更低。

4. 发明申请

WO2004081185A3 METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES 审中-公开
标题翻译：提高木质纤维素降解酶活性的方法
公开(公告)号：WO2004081185A3
公开(公告)日：2004-10-21
申请号：PCT/US2004007086
申请日：2004-03-08
申请人： ATHENIX CORP , BURDETTE JILL , VANDE BERG BRIAN , CARR BRIAN , DUCK NICHOLAS B , CAROZZI NADINE , KOZIEL MICHAEL G , PATEL PARESMA R
发明人： BURDETTE JILL , VANDE BERG BRIAN , CARR BRIAN , DUCK NICHOLAS B , CAROZZI NADINE , KOZIEL MICHAEL G , PATEL PARESMA R
IPC分类号： C12N20060101 , C12N9/04 , C12N9/18 , C12N9/20 , C12P7/10 , C12P19/02 , C12S3/00 , C13K1/02 , D06M10/00
CPC分类号： C12P7/10 , C08H8/00 , C12N9/0006 , C12N9/18 , C12N9/20 , D21C5/005 , D21C9/16 , D21C11/0007 , Y02E50/16
摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.
摘要翻译：提供了用于水解木质纤维素的方法，包括使木质纤维素与至少一种化学处理剂接触。还提供了用于预处理木质纤维素材料的方法，包括使材料与至少一种化学品接触。还提供了从植物材料中释放诸如酶，药物或营养制品的物质的方法。这些方法比现有方法更高效，更经济，毒性更小。

5. 发明申请

WO2006083891A3 AXMI-018, AXMI-020, AND AXMI-021, A FAMILY OF DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 审中-公开
标题翻译： AXMI-018，AXMI-020和AXMI-021，三肽内毒素基因家族及其使用方法
公开(公告)号：WO2006083891A3
公开(公告)日：2006-11-30
申请号：PCT/US2006003434
申请日：2006-01-31
申请人： ATHENIX CORP , CAROZZI NADINE , HARGISS TRACY , KOZIEL MICHAEL G , DUCK NICHOLAS B
发明人： CAROZZI NADINE , HARGISS TRACY , KOZIEL MICHAEL G , DUCK NICHOLAS B
IPC分类号： C07K14/325 , A01N63/02 , C12N15/00
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8281 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID N0: 2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, or 5, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4或6所示氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列，以及其变体和片段。

6. 发明申请

WO2010141141A3 AXMI-001, AXMI-002, AXMI-030, AXMI-035, AND AXMI-045: INSECTICIDAL PROTEINS FROM BACILLUS THURINGIENSIS AND METHODS FOR THEIR USE 审中-公开
标题翻译： AXMI-001，AXMI-002，AXMI-030，AXMI-035和AXMI-045：来自BACILLUS THURINGIENSIS的杀虫蛋白及其使用方法
公开(公告)号：WO2010141141A3
公开(公告)日：2011-05-05
申请号：PCT/US2010026914
申请日：2010-03-11
申请人： ATHENIX CORP , CAROZZI NADINE , DUCK NICHOLAS B , KOZIEL MICHAEL G , HARGISS TRACY , DETER REBEKAH , PETERS CHERYL L , VOLRATH SANDRA L , TOMSO DANIEL J
发明人： CAROZZI NADINE , DUCK NICHOLAS B , KOZIEL MICHAEL G , HARGISS TRACY , DETER REBEKAH , PETERS CHERYL L , VOLRATH SANDRA L , TOMSO DANIEL J
IPC分类号： C07K14/325 , A01H5/00 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 6-11, or the nucleotide sequence set forth in SEQ ID NO: 1-5, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：6-11所示的氨基酸序列的核苷酸序列，或SEQ ID NO：1-5所示的核苷酸序列，以及变体和片段。

7. 发明申请

WO2005021585B1 AXMI-003, A DELTA-ENDOTOXIN GENE AND METHODS FOR ITS USE 审中-公开
公开(公告)号：WO2005021585B1
公开(公告)日：2005-08-25
申请号：PCT/US2004028024
申请日：2004-08-27
申请人： ATHENIX CORP , CAROZZI NADINE , HARGISS TRACY , KOZIEL MICHAEL G , DUCK NICHOLAS B , CARR BRIAN
发明人： CAROZZI NADINE , HARGISS TRACY , KOZIEL MICHAEL G , DUCK NICHOLAS B , CARR BRIAN
IPC分类号： A01N63/02 , C07H21/04 , C07K14/325 , C12N15/82
CPC分类号： C07H21/04 , C07K14/325
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.

8. 发明申请

WO2005003362A2 METHODS TO CONFER HERBICIDE RESISTANCE 审中-公开
标题翻译：除草剂抗性的方法
公开(公告)号：WO2005003362A2
公开(公告)日：2005-01-13
申请号：PCT/US2004007169
申请日：2004-03-10
申请人： ATHENIX CORP , HAMMER PHILIP E , HINSON TODD K , DUCK NICHOLAS B , KOZIEL MICHAEL G
发明人： HAMMER PHILIP E , HINSON TODD K , DUCK NICHOLAS B , KOZIEL MICHAEL G
IPC分类号： C12N15/82
CPC分类号： C12N15/8275 , C12N15/8274
摘要： Compositions and methods for conferring herbicide resistance to plant cells and bacterial cells are provided. The methods comprise transforming the cells with nucleotide sequences encoding herbicide resistance genes. In particular, herbicide resistance is conferred by expression of proteins with homology to decarboxylase enzymes. Compositions comprise transformed plants, plant tissues, and seeds, as well as transformed bacterial cells.
摘要翻译：提供了赋予植物细胞和细菌细胞除草剂抗性的组合物和方法。该方法包括用编码除草剂抗性基因的核苷酸序列转化细胞。具体而言，通过表达与脱羧酶同源的蛋白质赋予除草剂抗性。组合物包含转化的植物，植物组织和种子，以及转化的细菌细胞。

9. 发明申请

WO2004074462A2 DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 审中-公开
标题翻译： DELTA-内毒素基因及其使用方法
公开(公告)号：WO2004074462A2
公开(公告)日：2004-09-02
申请号：PCT/US2004005829
申请日：2004-02-20
申请人： ATHENIX CORP , CAROZZI NADINE , HARGISS TRACY , KOZIEL MICHAEL G , DUCK NICHOLAS B , CARR BRIAN
发明人： CAROZZI NADINE , HARGISS TRACY , KOZIEL MICHAEL G , DUCK NICHOLAS B , CARR BRIAN
IPC分类号： C07K14/325 , C12N15/82 , C12N
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in in SEQ ID NOS:3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 27, and 29, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, 4, 6, 8, 10, 12, 13, 15, 17, 19, 21, 23, 25, 26, and 28, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,5,7,9,11,14,16,18,20,22,24,27中所示的氨基酸序列的核苷酸序列和29，以及SEQ ID NO：1,2,4,6,8,10,12,13,15,17,19,21,23,25,26和28所示的核苷酸序列，以及作为其变体和片段。

10. 发明申请

WO2007147096A3 A FAMILY OF PESTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译：一种农药蛋白质及其使用方法
公开(公告)号：WO2007147096A3
公开(公告)日：2008-07-24
申请号：PCT/US2007071298
申请日：2007-06-15
申请人： ATHENIX CORP , KOZIEL MICHAEL G , DESAI NALINI , DETER REBEKAH , TOMSO DANIEL JOHN , HARGISS TRACY , DUCK NICHOLAS B , CAROZZI NADINE , GUO RONG
发明人： KOZIEL MICHAEL G , DESAI NALINI , DETER REBEKAH , TOMSO DANIEL JOHN , HARGISS TRACY , DUCK NICHOLAS B , CAROZZI NADINE , GUO RONG
IPC分类号： C07K14/195 , C07K14/32 , C12N15/82
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-50047，B-30959，B-30960，B-30943，B-50048或B-50048，以及它们的变体和片段。

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