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1. 发明申请

WO2004081185B1 METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES 审中-公开
标题翻译：提高木糖醇降解酶活性的方法
公开(公告)号：WO2004081185B1
公开(公告)日：2004-12-23
申请号：PCT/US2004007086
申请日：2004-03-08
申请人： ATHENIX CORP , BURDETTE JILL , VANDE BERG BRIAN , CARR BRIAN , DUCK NICHOLAS B , CAROZZI NADINE , KOZIEL MICHAEL G , PATEL PARESMA R
发明人： BURDETTE JILL , VANDE BERG BRIAN , CARR BRIAN , DUCK NICHOLAS B , CAROZZI NADINE , KOZIEL MICHAEL G , PATEL PARESMA R
IPC分类号： C12N20060101 , C12N9/04 , C12N9/18 , C12N9/20 , C12P7/10 , C12P19/02 , C12S3/00 , C13K1/02 , D06M10/00
CPC分类号： C12P7/10 , C08H8/00 , C12N9/0006 , C12N9/18 , C12N9/20 , D21C5/005 , D21C9/16 , D21C11/0007 , Y02E50/16
摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.
摘要翻译：提供水解木质纤维素的方法，包括使木质纤维素与至少一种化学处理接触。还提供了用于预处理木质纤维素材料的方法，包括使材料与至少一种化学品接触。还提供了用于从植物材料释放诸如酶，药物或营养品的物质的方法。这些方法比目前的方法更有效，更经济，毒性更低。

2. 发明申请

WO2004081185A3 METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES 审中-公开
标题翻译：提高木质纤维素降解酶活性的方法
公开(公告)号：WO2004081185A3
公开(公告)日：2004-10-21
申请号：PCT/US2004007086
申请日：2004-03-08
申请人： ATHENIX CORP , BURDETTE JILL , VANDE BERG BRIAN , CARR BRIAN , DUCK NICHOLAS B , CAROZZI NADINE , KOZIEL MICHAEL G , PATEL PARESMA R
发明人： BURDETTE JILL , VANDE BERG BRIAN , CARR BRIAN , DUCK NICHOLAS B , CAROZZI NADINE , KOZIEL MICHAEL G , PATEL PARESMA R
IPC分类号： C12N20060101 , C12N9/04 , C12N9/18 , C12N9/20 , C12P7/10 , C12P19/02 , C12S3/00 , C13K1/02 , D06M10/00
CPC分类号： C12P7/10 , C08H8/00 , C12N9/0006 , C12N9/18 , C12N9/20 , D21C5/005 , D21C9/16 , D21C11/0007 , Y02E50/16
摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.
摘要翻译：提供了用于水解木质纤维素的方法，包括使木质纤维素与至少一种化学处理剂接触。还提供了用于预处理木质纤维素材料的方法，包括使材料与至少一种化学品接触。还提供了从植物材料中释放诸如酶，药物或营养制品的物质的方法。这些方法比现有方法更高效，更经济，毒性更小。

3. 发明申请

WO2008105905A2 METHODS AND COMPOSITIONS FOR IMPROVED ENZYME ACTIVITY IN TRANSGENIC PLANTS 审中-公开
标题翻译：提高转基因植物酶活性的方法和组合物
公开(公告)号：WO2008105905A2
公开(公告)日：2008-09-04
申请号：PCT/US2007073988
申请日：2007-07-20
申请人： ATHENIX CORP , KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
发明人： KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
IPC分类号： A01H1/00 , A01H5/00
CPC分类号： C12N15/8275
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译：提供了用于在植物，植物细胞，组织和种子中的广泛生理谱上增加酶活性的组合物和方法。组合物包括植物或植物部分，所述植物或植物部分包含两种或更多种编码多肽的多核苷酸，所述多核苷酸在多核苷酸单独表达时比在更广泛的生理光谱中有活性进一步提供了包含这些多核苷酸分子的载体以及包含该载体的宿主细胞。组合物还包含转化的细菌，植物，植物细胞，组织和种子。另外，提供了用于生产本发明的植物，植物细胞，组织和种子的方法。还提供了增加植物产量的方法和赋予植物对除草剂抗性的方法。

4. 发明申请

WO2007103768A2 METHODS AND COMPOSITIONS FOR IMPROVED ENZYME ACTIVITY IN TRANSGENIC PLANT 审中-公开
标题翻译：在转基因植物中改善酶活性的方法和组合物
公开(公告)号：WO2007103768A2
公开(公告)日：2007-09-13
申请号：PCT/US2007063132
申请日：2007-03-02
申请人： ATHENIX CORP , KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
发明人： KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
IPC分类号： A01H5/00
CPC分类号： C12N15/8261 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译：提供了用于增加植物，植物细胞，组织和种子中广泛生理光谱的酶活性的组合物和方法。组合物包括植物或植物部分，其包含两个或更多个编码与更多生理光谱相关的多肽的多核苷酸，而不是单个表达。还提供了包含这些多核苷酸分子的载体以及包含载体的宿主细胞。组合物还包括转化的细菌，植物，植物细胞，组织和种子。此外，提供了用于制备本发明的植物，植物细胞，组织和种子的方法。还提供了增加植物产量的方法和赋予植物中除草剂抗性的方法。

5. 发明申请

WO2007082269A9 EPSP SYNTHASE ENZYME DOMAINS CONFERRING GLYPHOSATE RESISTANCE 审中-公开
标题翻译： EPSP SYNTHASE酶结构域赋予甘油三酯抗性
公开(公告)号：WO2007082269A9
公开(公告)日：2007-10-04
申请号：PCT/US2007060396
申请日：2007-01-11
申请人： ATHENIX CORP , CARR BRIAN , HAMMER PHILIP E , HINSON TODD K , VANDE BERG BRIAN
发明人： CARR BRIAN , HAMMER PHILIP E , HINSON TODD K , VANDE BERG BRIAN
IPC分类号： C07K14/435
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include novel EPSP synthase enzymes and nucleic acid molecules encoding such enzymes, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子对草甘膦耐受性的组合物和方法。组合物包括新颖的EPSP合酶和编码这些酶的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。新蛋白质包含至少一个选自本文提供的结构域的序列结构域。这些序列结构域可用于鉴定具有草甘膦抗性活性的EPSP合酶。

6. 发明申请

WO2008002964A3 GRG33, GRG35, GRG36, GRG37, GRG38, GRG39, AND GRG50: NOVEL EPSP SYNTHASE GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GRG33，GRG35，GRG36，GRG37，GRG38，GRG39，和GRG50：新型EPSP合成基因抑制抗药性
公开(公告)号：WO2008002964A3
公开(公告)日：2008-07-10
申请号：PCT/US2007072206
申请日：2007-06-27
申请人： ATHENIX CORP , PETERS CHERYL L , VANDE BERG BRIAN , CARR BRIAN , TOMSO DANIEL JOHN
发明人： PETERS CHERYL L , VANDE BERG BRIAN , CARR BRIAN , TOMSO DANIEL JOHN
IPC分类号： C12N15/82 , A01H1/00 , C12Q1/68
CPC分类号： C12N9/1085 , C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO: 1, 3, 4, 6, 1, 9, 10, 12, 13, 15, 17, 18, 20, 21, or 23, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, 5, 8, 11, 14, 16, 19, or 22, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30932, B-30933, B-30934, B-30945, B-30946, B-30947, or B-30948, as well as variants and fragments thereof.
摘要翻译：提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，本发明提供了分离的核酸分子，其包含SEQ ID NO：1,3,4,6，1,9,10,12,13,15,17,18,20,21中所示的核苷酸序列或23，编码SEQ ID NO：2,5,8,11,14,16,19或22所示的氨基酸序列的核苷酸序列，将存在细菌宿主中的除草剂抗性核苷酸序列作为登录号NRRL B-30932，B-30933，B-30934，B-30945，B-30946，B-30947或B-30948，以及其变体和片段。

7. 发明申请

WO2008002962A2 GRG32: A NOVEL EPSP SYNTHASE GENE CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GRG32：一种新的EPSP合成酶基因，其抗除草剂抗性
公开(公告)号：WO2008002962A2
公开(公告)日：2008-01-03
申请号：PCT/US2007072203
申请日：2007-06-27
申请人： ATHENIX CORP , PETER CHERYL , VANDE BERG BRIAN , CARR BRIAN , TOMSO DANIEL JOHN
发明人： PETER CHERYL , VANDE BERG BRIAN , CARR BRIAN , TOMSO DANIEL JOHN
IPC分类号： C12N15/82
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO: 1 or 14, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30931, as well as variants and fragments thereof.
摘要翻译：提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，本发明提供了分离的核酸分子，其包含SEQ ID NO：1或14所示的核苷酸序列，编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列，作为登录号NRRL B-30931的细菌宿主，以及其变体和片段。

8. 发明申请

WO2007064828A3 GRG23 AND GRG51 GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GRG23和GRG51基因对抗药性的抵抗
公开(公告)号：WO2007064828A3
公开(公告)日：2007-08-02
申请号：PCT/US2006045908
申请日：2006-12-01
申请人： ATHENIX CORP , PETERS CHERYL L , HINSON JILL , HAMMER PHILIP E , VANDE BERG BRIAN , SCHOUTEN LAURA COOPER , CARR BRIAN
发明人： PETERS CHERYL L , HINSON JILL , HAMMER PHILIP E , VANDE BERG BRIAN , SCHOUTEN LAURA COOPER , CARR BRIAN
IPC分类号： C12N15/54 , A01H5/00 , C12N5/10 , C12N9/10 , C12N15/82 , C12Q1/48
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds.. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID N0:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO: 1 , 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦或多肽的分离的多核苷酸。另外，包含对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示氨基酸序列或SEQ ID NO：1,3或5所示核苷酸序列的核苷酸序列的分离多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

9. 发明申请

WO2007146765A3 NOVEL EPSP SYNTHASE GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译：新的EPSP合成基因决定抗药性
公开(公告)号：WO2007146765A3
公开(公告)日：2008-07-31
申请号：PCT/US2007070683
申请日：2007-06-08
申请人： ATHENIX CORP , CARR BRIAN , VANDE BERG BRIAN , TOMSO DANIEL JOHN , PETERS CHERYL
发明人： CARR BRIAN , VANDE BERG BRIAN , TOMSO DANIEL JOHN , PETERS CHERYL
IPC分类号： C12N9/10
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 4, 6, 8, 10, 12, or 14 or the nucleotide sequence set forth in SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13, 28, 29, 30, 31, 32, 33, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,6,8,10,12或14所示氨基酸序列的核苷酸序列或SEQ ID NOS ：1，3，5，7，9，11，13，28，29，30，31，32，33或34。

10. 发明申请

WO2007082269B1 EPSP SYNTHASE ENZYME DOMAINS CONFERRING GLYPHOSATE RESISTANCE 审中-公开
标题翻译： EPSP SYNTHASE ENZYME域保留谷氨酸电阻
公开(公告)号：WO2007082269B1
公开(公告)日：2008-05-08
申请号：PCT/US2007060396
申请日：2007-01-11
申请人： ATHENIX CORP , CARR BRIAN , HAMMER PHILIP E , HINSON TODD K , VANDE BERG BRIAN
发明人： CARR BRIAN , HAMMER PHILIP E , HINSON TODD K , VANDE BERG BRIAN
IPC分类号： C12N15/82 , A01H5/10 , C12N9/10 , C12N15/54
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include novel EPSP synthase enzymes and nucleic acid molecules encoding such enzymes, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.
摘要翻译：提供了在细菌，植物，植物细胞，组织和种子中赋予草甘膦耐受性的组合物和方法。组合物包括新型EPSP合成酶和编码这种酶的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。新型蛋白质包含选自本文提供的结构域的至少一个序列结构域。这些序列结构域可用于鉴定具有草甘膦抗性活性的EPSP合成酶。

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