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    • 3. 发明申请
    • SYSTEM AND METHOD FOR BIOLOGICAL APPLICATIONS USING PRE-DETERMINED SIZED NANOPARTICLES
    • 使用预先确定的尺寸纳米颗粒的生物应用的系统和方法
    • US20170071535A1
    • 2017-03-16
    • US15342262
    • 2016-11-03
    • ARkival Technology Corp.
    • Ronald D. Weiss
    • A61B5/00B03C1/025A61K49/18B03C1/30A61B5/05A61B5/055
    • A61B5/4839A61B5/0035A61B5/0515A61B5/055A61B5/7246A61B2562/0285A61K49/0002A61K49/1818B03C1/025B03C1/30B03C2201/18B03C2201/26
    • The invention features an apparatus for producing a fluid stream having plurality of nanoparticles in the fluid stream. The apparatus includes a source configured to provide a fluid stream having a first randomly sized distribution of a plurality of nanoparticles; a flow control zone configured to receive the fluid stream from the source and to control the fluid stream to produce a controlled fluid stream having a selected flow rate; a separation zone configured to receive and to separate the selectively controlled fluid stream into at least one separated fluid stream having a non-randomly sized distribution of nanoparticles; and a collection zone capable of receiving the separated fluid stream according to at least one non-random sized distribution of nanoparticles to produce at least one collected stream. The apparatus is configured for a continuous flow of the fluid stream. A size of a nanoparticle can be related to an intrinsic core diameter, a hydrodynamic diameter, and a combination of intrinsic core diameter and hydrodynamic diameter measurements. The nanoparticles can include non-magnetic nanoparticles, partially magnetic nanoparticles, magnetic nanoparticles, superparamagnetic nanoparticles, and a combination of at least two different nanoparticle types. The invention also features methods for producing said fluid streams. The invention further features apparatus and methods for cancer confirmation and targeted therapeutic drug development.
    • 本发明的特征在于一种用于在流体流中产生具有多个纳米颗粒的流体流的装置。 该装置包括:源,其配置成提供具有多个纳米颗粒的第一随机大小分布的流体流; 流动控制区域,其被配置为从所述源接收流体流并且控制所述流体流以产生具有选定流量的受控流体流; 分离区,被配置为接收并且将选择性控制的流体流分离成具有非随机大小的纳米颗粒分布的至少一个分离的流体流; 以及能够根据至少一个非随机大小的纳米颗粒分布接收分离的流体流以产生至少一个收集的流的收集区。 该装置构造成用于流体流的连续流动。 纳米颗粒的尺寸可以与本征芯直径,流体动力学直径以及本征芯直径和流体动力学直径测量的组合有关。 纳米颗粒可以包括非磁性纳米颗粒,部分磁性纳米颗粒,磁性纳米颗粒,超顺磁性纳米颗粒,以及至​​少两种不同纳米颗粒类型的组合。 本发明还涉及生产所述流体流的方法。 本发明还涉及用于癌症确认和靶向治疗药物开发的装置和方法。
    • 5. 发明申请
    • APPARATUS AND A METHOD FOR SORTING A PARTICULATE MATERIAL
    • 装置和分配颗粒材料的方法
    • US20140367312A1
    • 2014-12-18
    • US14355996
    • 2012-08-06
    • John Clarence BoxVladimir Arkadievich Golovanevskiy
    • John Clarence BoxVladimir Arkadievich Golovanevskiy
    • B03C1/025B03C1/00B03C1/26
    • B03C1/025B03C1/002B03C1/0335B03C1/035B03C1/26B03C2201/20
    • The present disclosure provides an apparatus for sorting a particulate material. The apparatus comprises a receiving portion for receiving the particulate material having a particle size within a predetermined range of sizes. The apparatus also comprises a distributor for receiving the particulate material at an upper end thereof and having a sloped distribution surface along which, in use, the particulate material passes substantially by gravity. A surface area of the sloped distribution surface increases towards a lower end of the distributor to facilitate a monolayer feed stream of the particles exiting and falling from a lower end of the distributor distributed as a curtain-like stream of particles. The apparatus further comprises a magnetic element for generating a magnetic force that is directed such that the particles of the falling feed stream that exit the lower end of the distributor have pathways that depend on magnetic properties of the particles. The apparatus also comprises sorting portions for sorting the particles based on the pathways of the particles.
    • 本公开提供了一种用于分选颗粒材料的设备。 该装置包括用于接收具有在预定尺寸范围内的粒度的颗粒材料的接收部分。 该设备还包括用于在其上端处接收颗粒材料并具有倾斜分布表面的分配器,在使用中,颗粒材料沿着该倾斜分布表面基本上通过重力通过。 倾斜分布表面的表面积朝向分配器的下端增加,以促进颗粒的单层进料流从分配器的下端排出并且作为帷幕状的颗粒分布分布。 该装置还包括用于产生磁力的磁性元件,该磁力被定向成使得离开分配器的下端的下降的进料流的颗粒具有取决于颗粒的磁性的通路。 该装置还包括基于颗粒的路径分选颗粒的分选部分。
    • 8. 发明授权
    • Quantitative cell analysis methods employing magnetic separation
    • 使用磁分离的定量细胞分析方法
    • US5993665A
    • 1999-11-30
    • US867008
    • 1997-06-02
    • Leon W. M. M. TerstappenPaul A. Liberti
    • Leon W. M. M. TerstappenPaul A. Liberti
    • B03C1/025B01D35/06B03C1/034B03C1/035G01N33/533G01N33/543B01D35/00
    • B03C1/025B01L3/502B03C1/034B03C1/035G01N27/745G01N33/54326G01N33/54366B01L2400/043Y10T436/108331Y10T436/25375
    • A method of quantitative analysis of microscopic biological specimens in a fluid medium is disclosed in which the specimens are rendered magnetically responsive by immunospecific binding with ferromagnetic colloid. A known quantity of magnetically-responsive marker particles are added to the fluid medium. The fluid medium is then subjected to a magnetic separation process, to collect the magnetic species from the fluid. The collected species are resuspended in a second fluid medium, and the relative quantities thereof are enumerated to determine the concentration of the desired biological specimen in the first fluid medium. The marker particles may comprise magnetic particles having a relatively large magnetic moment, a magnetic moment approximately equal to the magnetically-labelled biological speciment of interest, or both in order to compensate the determination for variations in immunospecific binding affinity and/or magnetic collection efficiency. The present method is useful for conducting hematological assays, such as red blood cell counts and related determinations. In the case of red blood cells, the specimens may be rendered magnetically responsive by reduction of the iron atoms present in hemoglobin. The enumeration step of the method may be conducted with such equipment as a flow cytometer, or by a novel optical scanning magnetic cell immobilization apparatus.
    • 公开了一种流体介质中微观生物样本的定量分析方法,其中通过与铁磁性胶体的免疫特异性结合使样本呈现磁响应。 将已知量的磁响应标记颗粒添加到流体介质中。 然后对流体介质进行磁分离处理,以从流体中收集磁性物质。 将收集的物质重新悬浮在第二流体培养基中,并列举其相对量以确定第一流体培养基中所需生物样品的浓度。 标记颗粒可以包括具有相对大的磁矩,大约等于感兴趣的磁性标记的生物学物理学的磁矩或两者的磁性颗粒,以补偿免疫特异性结合亲和力和/或磁收集效率的变化的确定。 本方法可用于进行血液学测定,如红细胞计数和相关测定。 在红细胞的情况下,可以通过还原血红蛋白中存在的铁原子来使样品呈现磁响应性。 该方法的计数步骤可以用流式细胞仪等设备进行,也可以通过新的光学扫描磁性细胞固定装置进行。
    • 9. 发明授权
    • Magnetic immobilization and manipulation of biological entities
    • 磁固定和操纵生物实体
    • US5876593A
    • 1999-03-02
    • US931067
    • 1997-09-15
    • Paul A. LibertiYuzhou Wang
    • Paul A. LibertiYuzhou Wang
    • B01D35/06B03C1/00B03C1/01B03C1/025B03C1/032B03C1/033B03C1/034B03C1/035B03C1/28C12M3/00G01N33/483G01N33/543G01N33/569G01N35/00G01N35/02
    • B03C1/002B03C1/01B03C1/025B03C1/032B03C1/0332B03C1/034B03C1/035C12M47/04G01N33/54326G01N33/56972G01N35/026G01N2333/70589G01N35/0098Y10T436/108331Y10T436/25375
    • Biological entities such as cells, microbes, or components thereof are labeled with a magnetic colloid containing microscopic magnetic particles. The magnetic particles have a coating capable of biospecific or non-specific binding with the entities. An immobilization apparatus includes a non-magnetic vessel having a ferromagnetic collection structure for attracting the entities toward a collection surface upon which the magnetically labeled entities are immobilized subsequent to placement of the vessel on a support between two magnets. The ferromagnetic collection structure preferably has a sharp edge or high curvature for intensifying the magnetic field and for collecting the entities in a monolayer. The vessel includes an un-obstructed observation path so that immobilized entities may be observed and/or manipulated. The ferromagnetic collection structure may be arranged in various two dimensional patterns to provide a desired collection configuration. The apparatus may further have inlet and outlet ports for allowing a flow of liquid reagent through the vessel for washing or straining the immobilized entities. The support can be translated so that a shoulder on the collection structure concentrates the collected entities by movement of the shoulder in a transverse direction relative to the magnetic field.
    • PCT No.PCT / US93 / 11087 Sec。 371日期1995年04月24日 102(e)1995年4月24日PCT PCT 1997年9月15日PCT公布。 公开号WO94 / 11078 日期1994年5月26日生物实体如细胞,微生物或其组分用含有微观磁性颗粒的磁性胶体标记。 磁性颗粒具有能够与实体进行生物特异性或非特异性结合的涂层。 固定装置包括具有铁磁收集结构的非磁性容器,用于在将容器放置在两个磁体之间的支撑件上时,将实体吸引到收集表面,在该收集表面上磁性标记的实体被固定。 铁磁收集结构优选地具有尖锐的边缘或高曲率,用于增强磁场和用于在单层中收集实体。 容器包括未阻挡的观察路径,使得可以观察和/或操纵固定的实体。 铁磁收集结构可以以各种二维图案布置以提供期望的收集配置。 该装置还可以具有用于允许液体试剂流过容器的入口和出口,用于洗涤或使固定的实体变窄。 支撑件可以平移,使得收集结构上的肩部通过肩部相对于磁场的横向方向的移动来集中收集的实体。