专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5876593A Magnetic immobilization and manipulation of biological entities 失效
标题翻译：磁固定和操纵生物实体
公开(公告)号：US5876593A
公开(公告)日：1999-03-02
申请号：US931067
申请日：1997-09-15
申请人： Paul A. Liberti , Yuzhou Wang
发明人： Paul A. Liberti , Yuzhou Wang
IPC分类号： B01D35/06 , B03C1/00 , B03C1/01 , B03C1/025 , B03C1/032 , B03C1/033 , B03C1/034 , B03C1/035 , B03C1/28 , C12M3/00 , G01N33/483 , G01N33/543 , G01N33/569 , G01N35/00 , G01N35/02
CPC分类号： B03C1/002 , B03C1/01 , B03C1/025 , B03C1/032 , B03C1/0332 , B03C1/034 , B03C1/035 , C12M47/04 , G01N33/54326 , G01N33/56972 , G01N35/026 , G01N2333/70589 , G01N35/0098 , Y10T436/108331 , Y10T436/25375
摘要： Biological entities such as cells, microbes, or components thereof are labeled with a magnetic colloid containing microscopic magnetic particles. The magnetic particles have a coating capable of biospecific or non-specific binding with the entities. An immobilization apparatus includes a non-magnetic vessel having a ferromagnetic collection structure for attracting the entities toward a collection surface upon which the magnetically labeled entities are immobilized subsequent to placement of the vessel on a support between two magnets. The ferromagnetic collection structure preferably has a sharp edge or high curvature for intensifying the magnetic field and for collecting the entities in a monolayer. The vessel includes an un-obstructed observation path so that immobilized entities may be observed and/or manipulated. The ferromagnetic collection structure may be arranged in various two dimensional patterns to provide a desired collection configuration. The apparatus may further have inlet and outlet ports for allowing a flow of liquid reagent through the vessel for washing or straining the immobilized entities. The support can be translated so that a shoulder on the collection structure concentrates the collected entities by movement of the shoulder in a transverse direction relative to the magnetic field.
摘要翻译： PCT No.PCT / US93 / 11087 Sec。 371日期1995年04月24日 102（e）1995年4月24日PCT PCT 1997年9月15日PCT公布。公开号WO94 / 11078 日期1994年5月26日生物实体如细胞，微生物或其组分用含有微观磁性颗粒的磁性胶体标记。磁性颗粒具有能够与实体进行生物特异性或非特异性结合的涂层。固定装置包括具有铁磁收集结构的非磁性容器，用于在将容器放置在两个磁体之间的支撑件上时，将实体吸引到收集表面，在该收集表面上磁性标记的实体被固定。铁磁收集结构优选地具有尖锐的边缘或高曲率，用于增强磁场和用于在单层中收集实体。容器包括未阻挡的观察路径，使得可以观察和/或操纵固定的实体。铁磁收集结构可以以各种二维图案布置以提供期望的收集配置。该装置还可以具有用于允许液体试剂流过容器的入口和出口，用于洗涤或使固定的实体变窄。支撑件可以平移，使得收集结构上的肩部通过肩部相对于磁场的横向方向的移动来集中收集的实体。

2. 发明授权

US5466574A Apparatus and methods for magnetic separation featuring external magnetic means 失效
标题翻译：用于磁分离的装置和方法，具有外部磁性装置
公开(公告)号：US5466574A
公开(公告)日：1995-11-14
申请号：US6071
申请日：1993-01-15
申请人： Paul A. Liberti , Yuzhou Wang , Weixin Tang , Brian P. Feeley , Dhanesh I. Gohel
发明人： Paul A. Liberti , Yuzhou Wang , Weixin Tang , Brian P. Feeley , Dhanesh I. Gohel
IPC分类号： B03C1/00 , A23L3/32 , B01D35/06 , B01D43/00 , B03C1/025 , B03C1/035 , G01N33/543 , G01N33/553 , G01N33/577 , G01N35/00 , C12Q1/70
CPC分类号： B03C1/286 , A23L3/32 , B03C1/035 , G01N33/54326 , B03C2201/18 , B03C2201/26 , G01N35/0098 , Y10S435/971 , Y10S436/807 , Y10S436/824 , Y10S436/827 , Y10S436/828
摘要： A magnetic separator for isolating magnetically-labeled substances of interest, such as immunological agents, from a non-magnetic test medium using a method of high gradient magnetic separation. The target substance is contacted with microscopic magnetic particles having a receptor for binding with the target substance. The test medium containing the magnetic particles is held in a non-magnetic container and placed into a gap within an arrangement of magnets for causing the magnetic particles to adhere to selected locations upon the interior wall of the container. The quantity of magnetic particles may be controlled to cause the magnetic particles collected upon the interior wall to form a monolayer. The magnets are arranged upon a yoke which may provide linear, surrounding multipolar, or partially surrounding multipolar configurations of magnetic pole faces about the gap. The magnetic pole faces may be of differing cross-sectional areas to enhance the magnetic field gradient in regions adjacent to the smaller pole faces. The method and apparatus may be used for such investigations as enzyme-labeled competitive immunoassay and "sandwich" immunoassays.
摘要翻译：一种使用高梯度磁选法从非磁性测试介质中分离磁性标记物质的磁分离器，例如免疫学试剂。目标物质与具有与靶物质结合的受体的微观磁性粒子接触。将包含磁性颗粒的测试介质保持在非磁性容器中并放置在磁体布置内的间隙中，以使磁性颗粒粘附到容器内壁上的选定位置。可以控制磁性颗粒的量以使收集在内壁上的磁性颗粒形成单层。磁体布置在磁轭上，该磁轭可围绕间隙提供线性的，围绕多极的或部分围绕的多极配置的磁极面。磁极面可以具有不同的横截面积，以增强与较小极面相邻的区域中的磁场梯度。该方法和装置可用于诸如酶标记的竞争性免疫测定和“三明治”免疫测定的这种研究。

3. 发明授权

US6013532A Methods for magnetic immobilization and manipulation of cells 失效
标题翻译：磁固定和细胞操作方法
公开(公告)号：US6013532A
公开(公告)日：2000-01-11
申请号：US878617
申请日：1997-06-19
申请人： Paul A. Liberti , Yuzhou Wang
发明人： Paul A. Liberti , Yuzhou Wang
IPC分类号： B03C1/00 , B03C1/01 , B03C1/025 , B03C1/032 , B03C1/033 , B03C1/034 , B03C1/035 , C12M3/00 , G01N33/483 , G01N33/543 , G01N33/569 , G01N35/00 , G01N35/02 , G01N33/553 , G01N33/536 , G01N33/566
CPC分类号： G01N35/026 , B03C1/002 , B03C1/01 , B03C1/025 , B03C1/032 , B03C1/0332 , B03C1/034 , B03C1/035 , G01N33/54326 , G01N33/56972 , G01N2333/70589 , G01N35/0098
摘要： Microbiological entities, such as cells or microbes, are immobilized in a single-file linear array for optical analysis. Colloidal magnetic particles having a binding agent, such as ligand for attachment with a corresponding receptor, are bound to the entities suspended in a fluid medium. The fluid medium is placed into a vessel having a ferromagnetic capture structure including an elongated linear collection surface with a diameter less than that of the microbiological species of interest. The vessel is placed into a magnetic field for inducing a magnetic gradient in a region along the collection surface of the ferromagnetic capture structure. The magnetically-labeled entities are attracted toward the collection surface and immobilized thereon in a linear array. Microscopic optical and fluorescence observations, and sequential chemical reactions and mechanical manipulations may be performed on the line of immobilized entities. Methods of analysis for blood and immune cells, such as leukocytes and lymphocytes, are disclosed.
摘要翻译：诸如细胞或微生物的微生物实体被固定在用于光学分析的单文件线性阵列中。具有结合剂（例如用于与相应受体连接的配体）的胶体磁性颗粒与悬浮在流体介质中的实体结合。将流体介质置于具有铁磁捕获结构的容器中，该结构包括直径小于感兴趣的微生物种类的细长线性收集表面。将容器放置在磁场中，以在沿着铁磁捕获结构的收集表面的区域中引起磁梯度。磁标记的实体被吸引到收集表面并以线性阵列固定在其上。可以在固定化实体的线上进行微观光学和荧光观察，以及顺序化学反应和机械操作。公开了血液和免疫细胞如白细胞和淋巴细胞的分析方法。

4. 发明授权

US5795470A Magnetic separation apparatus 失效
标题翻译：磁选机
公开(公告)号：US5795470A
公开(公告)日：1998-08-18
申请号：US482636
申请日：1995-06-07
申请人： Yuzhou Wang , Weixin Tang , Paul A. Liberti
发明人： Yuzhou Wang , Weixin Tang , Paul A. Liberti
IPC分类号： A23L3/32 , B03C1/035 , G01N35/00 , B01D35/06
CPC分类号： A23L3/32 , B03C1/035 , G01N35/0098
摘要： A magnetic separation apparatus is disclosed herein. The apparatus includes a flow-through vessel defining a plenum and an inlet port for introducing fluid into the plenum. A hydrodynamic damping structure is provided between the inlet port and plenum for reducing turbulence in the fluid. A mounting device is provided with a slot therein for supporting and receiving the vessel. The mounting device also supports a first array of magnets along one side of the slot and arranged adjacent one another to face the slot with alternating polarity, and a second array of magnets along the opposite side of the slot from the first array arranged adjacent one another to face the slot with alternating polarity and confronting and magnetically opposing the magnets in the first array. The first and second arrays of magnets produce a magnetic field within the plenum having a field strength that is maximum at a location at the interior surface of a wall of the vessel and that is minimum at a geometrical center of the plenum so that magnetic particles in the fluid are attracted to and immobilized on the inner surface of the vessel.
摘要翻译：本文公开了磁分离装置。该装置包括限定通气室的流通容器和用于将流体引入通风室的入口端口。在入口和集气室之间设有流体动力学阻尼结构，以减少流体中的湍流。安装装置在其中设置有用于支撑和接收容器的狭槽。安装装置还支撑沿着槽的一侧的第一磁体阵列并彼此相邻地布置以与交替极性相对的槽，并且沿与狭槽相邻的第一阵列的相对侧的第二磁体阵列相邻布置面对具有交替极性的槽，并且与第一阵列中的磁体相对并且磁性地相对。第一和第二磁体阵列在集气室内产生磁场，其中磁场强度在容器的壁的内表面处的位置处是最大的，并且在集气室的几何中心处最小，使得磁性颗粒在流体被吸引并固定在容器的内表面上。

5. 发明授权

US5541072A Method for magnetic separation featuring magnetic particles in a multi-phase system 失效
标题翻译：在多相系统中磁性粒子的磁分离方法
公开(公告)号：US5541072A
公开(公告)日：1996-07-30
申请号：US228818
申请日：1994-04-18
申请人： Yuzhou Wang , Weixin Tang , William J. Cronin , Paul A. Liberti
发明人： Yuzhou Wang , Weixin Tang , William J. Cronin , Paul A. Liberti
IPC分类号： B03C1/01 , B03C1/28 , B03C1/32 , C12M3/00 , G01N33/543 , G01N35/00 , G01N35/02 , H01F1/44 , G01N33/567 , G01N33/569
CPC分类号： G01N35/026 , B03C1/01 , B03C1/288 , B03C1/32 , C12M47/04 , G01N33/54326 , H01F1/44 , G01N35/0098 , Y10S436/807 , Y10S436/824 , Y10T436/25375
摘要： Methods and devices are provided for separation of magnetic particles and/or magnetic-associated substances from non-magnetic associated substances and media. The methods are specifically applicable to biological separation, and utilize a phase phenomenon arising from mutual interactions between suspended magnetic particles and interactions with the suspension medium. The phenomenon is exploited to produce and maintain a distinct, structured phase of magnetic particles, or ferrophase, within a multi-phase liquid system. A ferrophase is established within a separation chamber prior to introducing therein a test sample containing the target substances to be separated. The formation of a ferrophase is used to transport target substances from regions of relatively low magnetic field gradient to regions of relatively high magnetic field gradient within a high-gradient magnetic separation apparatus. Such transport of the ferrophase is accomplished at greater speed than the transport of individual magnetic particles within a single liquid phase, thereby enabling a more effective separation of magnetic from non-magnetic components.
摘要翻译：提供了用于将磁性颗粒和/或磁性相关物质与非磁性相关物质和介质分离的方法和装置。该方法特别适用于生物分离，并利用由悬浮磁性颗粒与悬浮介质的相互作用引起的相位现象。这种现象被用于在多相液体系统内产生和维持磁性颗粒或热解酶的独特结构化阶段。在引入含有待分离的目标物质的测试样品之前，在分离室内建立热解酶。铁高杆菌的形成用于将靶物质从相对低的磁场梯度的区域输送到高梯度磁力分离装置内的相对高的磁场梯度的区域。富马酸酶的这种运输以比在单个液相内单个磁性颗粒的输送更快的速度来实现，从而能够更有效地将磁性与非磁性组分分离。

6. 发明授权

US5993665A Quantitative cell analysis methods employing magnetic separation 失效
标题翻译：使用磁分离的定量细胞分析方法
公开(公告)号：US5993665A
公开(公告)日：1999-11-30
申请号：US867008
申请日：1997-06-02
申请人： Leon W. M. M. Terstappen , Paul A. Liberti
发明人： Leon W. M. M. Terstappen , Paul A. Liberti
IPC分类号： B03C1/025 , B01D35/06 , B03C1/034 , B03C1/035 , G01N33/533 , G01N33/543 , B01D35/00
CPC分类号： B03C1/025 , B01L3/502 , B03C1/034 , B03C1/035 , G01N27/745 , G01N33/54326 , G01N33/54366 , B01L2400/043 , Y10T436/108331 , Y10T436/25375
摘要： A method of quantitative analysis of microscopic biological specimens in a fluid medium is disclosed in which the specimens are rendered magnetically responsive by immunospecific binding with ferromagnetic colloid. A known quantity of magnetically-responsive marker particles are added to the fluid medium. The fluid medium is then subjected to a magnetic separation process, to collect the magnetic species from the fluid. The collected species are resuspended in a second fluid medium, and the relative quantities thereof are enumerated to determine the concentration of the desired biological specimen in the first fluid medium. The marker particles may comprise magnetic particles having a relatively large magnetic moment, a magnetic moment approximately equal to the magnetically-labelled biological speciment of interest, or both in order to compensate the determination for variations in immunospecific binding affinity and/or magnetic collection efficiency. The present method is useful for conducting hematological assays, such as red blood cell counts and related determinations. In the case of red blood cells, the specimens may be rendered magnetically responsive by reduction of the iron atoms present in hemoglobin. The enumeration step of the method may be conducted with such equipment as a flow cytometer, or by a novel optical scanning magnetic cell immobilization apparatus.
摘要翻译：公开了一种流体介质中微观生物样本的定量分析方法，其中通过与铁磁性胶体的免疫特异性结合使样本呈现磁响应。将已知量的磁响应标记颗粒添加到流体介质中。然后对流体介质进行磁分离处理，以从流体中收集磁性物质。将收集的物质重新悬浮在第二流体培养基中，并列举其相对量以确定第一流体培养基中所需生物样品的浓度。标记颗粒可以包括具有相对大的磁矩，大约等于感兴趣的磁性标记的生物学物理学的磁矩或两者的磁性颗粒，以补偿免疫特异性结合亲和力和/或磁收集效率的变化的确定。本方法可用于进行血液学测定，如红细胞计数和相关测定。在红细胞的情况下，可以通过还原血红蛋白中存在的铁原子来使样品呈现磁响应性。该方法的计数步骤可以用流式细胞仪等设备进行，也可以通过新的光学扫描磁性细胞固定装置进行。

7. 发明授权

US5512332A Process of making resuspendable coated magnetic particles 失效
标题翻译：制备可重悬涂层磁性颗粒的方法
公开(公告)号：US5512332A
公开(公告)日：1996-04-30
申请号：US231379
申请日：1994-04-22
申请人： Paul A. Liberti , Steven P. Piccoli
发明人： Paul A. Liberti , Steven P. Piccoli
IPC分类号： A61K9/50 , A61K49/18 , B03C1/01 , C12N11/14 , G01N33/543 , H01F1/44 , H01F1/00
CPC分类号： A61K49/1875 , A61K49/1833 , A61K49/1836 , A61K49/1854 , A61K49/1857 , A61K49/186 , A61K49/1863 , A61K49/1869 , A61K9/5094 , B03C1/01 , C12N11/14 , G01N33/54333 , G01N33/5434 , G01N33/54393 , H01F1/445 , G01N2333/765 , G01N2446/20 , G01N2446/30 , G01N2446/80
摘要： A method is provided for direct coating of transiently stable, colloidal particulate magnetic substrates with various synthetic or naturally-occurring polymers, particularly biologically-active peptides and proteins. The resulting coated resuspendable, colloidal magnetic particles are particularly adapted for use in bioanalytical procedures involving affinity separation, or the like. There is also provided a method for selectively binding chemically-treated colloidal magnetic particles to polyelectrolytes, e.g., to facilitate extraction of nucleic acids from cell lysates.
摘要翻译：提供了一种用于使用各种合成或天然存在的聚合物，特别是生物活性肽和蛋白质直接涂覆瞬时稳定的胶体颗粒磁性底物的方法。所得涂覆的可再悬浮的胶体磁性颗粒特别适用于涉及亲和力分离的生物分析方法等。还提供了一种用于选择性地将化学处理的胶体磁性颗粒结合到聚电解质的方法，例如以便于从细胞裂解物中提取核酸。

8. 发明申请

US20100282788A1 ASYMMETRIC HANGER FOR SHORT AND LONG TROUSERS 审中-公开
标题翻译：不合适的短裤和长裤
公开(公告)号：US20100282788A1
公开(公告)日：2010-11-11
申请号：US12689538
申请日：2010-01-19
申请人： Paul A. Liberti
发明人： Paul A. Liberti
IPC分类号： A41D27/22
CPC分类号： A47G25/445
摘要： A method and device for hanging long or short pants or any other similar articles of clothing that contains a waistband are disclosed. The device, an asymmetric hanger, hangs folded pants from the waist and is composed of a finger adapted to insert into the top of a pair of trousers engaging the inside of the waistband along with a clamp that clasps the waistband from the outside near the opposite end of the folded pants and that exerts a force away from the finger. The device can be adapted to hang pants from a simple clothes pole to a variety of novel designs that display pants and facilitate removal or hanging.
摘要翻译：公开了一种用于悬挂长裤或短裤或任何其它类似的包含腰带的衣物的方法和装置。该装置是一种不对称的衣架，从腰部悬挂折叠的裤子，由一根手指组成，该手指适合于插入一条裤腰的上部，一条裤子与裤腰内侧接合折叠的裤子的末端，并且从手指施加力。该装置可以适用于将裤子从简单的衣架悬挂到各种新颖的设计，显示裤子和便于拆卸或挂起。

9. 发明授权

US5866099A Magnetic-polymer particles 失效
标题翻译：磁性聚合物颗粒
公开(公告)号：US5866099A
公开(公告)日：1999-02-02
申请号：US846575
申请日：1997-04-30
申请人： Charles S. Owen , John C. Silvia , Louis D'Angelo , Paul A. Liberti
发明人： Charles S. Owen , John C. Silvia , Louis D'Angelo , Paul A. Liberti
IPC分类号： A61B5/055 , A61K9/50 , A61K49/18 , C12N11/14 , G01N33/543
CPC分类号： A61K49/1818 , A61K9/5094 , C12N11/14 , G01N33/54333 , G01N33/5434 , G01N2333/765 , G01N2446/20 , G01N2446/30 , Y10S435/972 , Y10T428/2982
摘要： A magnetic-polymer particle, useful in immunoassay techniques and various other biological/medical applications is produced by coprecipitation of transition metals in the presence of a polymer having available coordination sites. These particles are capable of forming stable aqueous suspensions and may be easily resuspended after agglomeration.
摘要翻译：可用于免疫测定技术和各种其它生物/医学应用的磁性聚合物颗粒通过过渡金属在具有可用配位点的聚合物存在下共沉淀而产生。这些颗粒能够形成稳定的水性悬浮液，并且在附聚后可以容易地重新悬浮。

10. 发明授权

US5646001A Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof 失效
标题翻译：从其混合群体中亲和力结合分离和释放一个或多个选定的生物实体子集
公开(公告)号：US5646001A
公开(公告)日：1997-07-08
申请号：US395967
申请日：1995-02-28
申请人： Leon W. M. M. Terstappen , Galla C. Rao , Dhanesh I. Gohel , Brian P. Feeley , Steven Gross , Ellen S. Church , Paul A. Liberti
发明人： Leon W. M. M. Terstappen , Galla C. Rao , Dhanesh I. Gohel , Brian P. Feeley , Steven Gross , Ellen S. Church , Paul A. Liberti
IPC分类号： A23L3/32 , B03C1/035 , G01N33/543 , G01N33/569 , G01N35/00 , G01N33/567
CPC分类号： A23L3/32 , B03C1/035 , G01N33/54326 , G01N33/569 , G01N35/0098 , Y10S435/962 , Y10S435/973 , Y10S436/824 , Y10S436/825 , Y10T436/25
摘要： A method for separation of a mixture of biological entities into at least three distinct, subpopulations. Different antibodies are provided, with each antibody bound to a solid support in a unique manner such that by a manipulation of the physical or chemical environment, the bonds between the antibodies and the solid supports can be selectively broken. The mixed population of cells is incubated with the antibodies. The cells are magnetically separated from a test medium and collected in a monolayer upon a collection surface. Then by manipulation of the physicochemical environment, specific linkages can be broken and desired cell subpopulations released from the collection surface. This method has medically significant diagnostic and therapeutic applications, as entire cell types can be separated from non-malignant medically vital cell types. Cancer can be diagnosed, staged, and monitored. Genetic analysis from maternal blood, CVS, or amniocentesis samples is possible. Diseases such as AIDS, tuberculosis or hepatitis can be monitored. This invention also has utility in the fields of bone marrow transplantation, fetal cell research, in vitro fertilization, and gene therapy.
摘要翻译：将生物实体的混合物分离成至少三个不同的亚群体的方法。提供了不同的抗体，每种抗体以独特的方式结合到固体支持物上，使得通过操纵物理或化学环境，可以选择性地破坏抗体和固体支持物之间的键。将混合的细胞群与抗体一起孵育。将细胞与测试介质磁分离并收集在收集表面上的单层中。然后通过操纵物理化学环境，可以破坏特定的连接并且从收集表面释放所需的细胞亚群。该方法具有医学上显着的诊断和治疗应用，因为整个细胞类型可以与非恶性的医学上重要的细胞类型分离。可以诊断，分期和监测癌症。从母体血液，CVS或羊膜穿刺样品的遗传分析是可能的。可以监测艾滋病，结核病或肝炎等疾病。本发明还可用于骨髓移植，胎儿细胞研究，体外受精和基因治疗领域。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式