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1. 发明授权

US07939300B2 Device and method for high-throughput quantification of mRNA from whole blood 有权
标题翻译：从全血中高通量定量mRNA的装置和方法
公开(公告)号：US07939300B2
公开(公告)日：2011-05-10
申请号：US11803593
申请日：2007-05-15
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： C12P1/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12N15/1006 , C12N1/066 , C12Q1/6806 , C12Q1/6846 , C12Q2565/537 , C12Q2521/107 , C12Q2527/137 , C12Q2527/125
摘要： Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.
摘要翻译：公开了一种用于从全血中简单，可重复和高通量定量mRNA的方法，装置试剂盒和自动化系统。更具体地，方法，装置，试剂盒和自动化系统涉及附着于寡聚（dT）活化的多孔板的白细胞过滤器的组合。

2. 发明授权

US08569020B2 Method for simultaneously performing multiple amplification reactions 有权
标题翻译：进行多重核酸扩增程序的方法
公开(公告)号：US08569020B2
公开(公告)日：2013-10-29
申请号：US12241350
申请日：2008-09-30
申请人： Kelly G. Ammann , Ralph E. Burns , Ernest V. Hansberry , Glenn A. Horner , Cheryl A. Jakub , John E. Kling , Donald J. Nieglos , Robert E. Schneider , Robert J. Smith
发明人： Kelly G. Ammann , Ralph E. Burns , Ernest V. Hansberry , Glenn A. Horner , Cheryl A. Jakub , John E. Kling , Donald J. Nieglos , Robert E. Schneider , Robert J. Smith
IPC分类号： C12P1/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6834 , B01F9/0001 , B01F9/0014 , B01F9/0021 , B01F2009/0074 , B01L7/52 , B01L7/5255 , B01L9/06 , B01L2200/147 , B01L2300/1822 , B01L2300/1827 , B03C1/282 , B03C1/288 , B03C1/30 , B03C2201/26 , C12Q1/6813 , C12Q1/6832 , C12Q2537/10 , G01N35/0098 , G01N35/0099 , G01N35/025 , G01N35/1065 , G01N2035/00356 , G01N2035/00524 , G01N2035/0437 , G01N2035/0455 , G01N2035/103 , Y10T29/53657 , Y10T29/53683 , Y10T29/53843 , Y10T156/1105 , Y10T156/1906 , Y10T436/11 , Y10T436/111666 , Y10T436/113332 , Y10T436/114998 , Y10T436/119163 , Y10T436/2575
摘要： An automated method for performing multiple nucleic acid amplification procedures in multiple receptacles of a set of receptacles contained in an analyzer.
摘要翻译：一种用于在包含在分析器中的一组容器的多个容器中执行多个核酸扩增程序的自动化方法。

3. 发明授权

US07709232B2 Methods of quantitative detection of genetic recombinants and standard molecules for the methods 有权
标题翻译：方法定量检测遗传重组体和标准分子
公开(公告)号：US07709232B2
公开(公告)日：2010-05-04
申请号：US10423399
申请日：2003-04-25
申请人： Akihiro Hino , Takeshi Matsuoka , Hideo Kuribara , Tomoaki Yoshimura , Yoichiro Shindo , Satoshi Futo , Machiko Ogawa
发明人： Akihiro Hino , Takeshi Matsuoka , Hideo Kuribara , Tomoaki Yoshimura , Yoichiro Shindo , Satoshi Futo , Machiko Ogawa
IPC分类号： C12P1/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6851 , C12N15/8274 , C12N15/8275 , C12N15/8286 , Y02A40/162 , C12Q2525/113 , C12Q2545/101
摘要： The present invention relate to a method of detecting a genetic recombinant by using the PCR method. A method of quantitatively detecting method is provided whereby the total content ratio of the genetic recombinants and the individual content ratio of the genetic recombinant in a population containing plural genetic recombinant lines can be quantified. The method of the present invention comprises performing PCR for the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species corresponding to the recombinant using, as a standard molecule, a molecule containing the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species on the single molecule, and determining the content ratio of the number of molecules thereof.
摘要翻译：本发明涉及使用PCR方法检测遗传重组体的方法。提供了一种定量检测方法，其中可以量化含有多个遗传重组体的群体中遗传重组体的总含量比和遗传重组体的个体含量比。本发明的方法包括对与重组体对应的物种共有的DNA序列进行PCR，所述DNA序列用作重组体和内源性的特异性DNA分子作为标准分子，作为标准分子由单个分子上的物种共享的DNA序列，并确定其分子数目的含量比。

4. 发明授权

US07807447B1 Compositions and methods for exon profiling 有权
标题翻译：外显子分析的组成和方法
公开(公告)号：US07807447B1
公开(公告)日：2010-10-05
申请号：US09724538
申请日：2000-11-28
申请人： Daniel D. Shoemaker , Stewart Scherer , Stephen H. Friend
发明人： Daniel D. Shoemaker , Stewart Scherer , Stephen H. Friend
IPC分类号： C12M1/34 , C12M3/00 , C12Q1/68 , C12P1/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6837 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00707 , B01J2219/00722 , B01J2219/00743 , C12Q1/6809 , C40B40/06
摘要： The present invention provides methods for analyzing exon expression profiles of a cell or type of cell. In the invention, the expression levels of a plurality of individual exons or multiexons for each of a plurality of genes in the genome of an organism are measured and analyzed to determine the biological state, such as the exon expression state or transcriptional state, of the cell or type of cell. The methods of the invention are useful for determination of alternative RNA splicing in a plurality of genes. The invention also provides nucleic acid probe arrays for determining in parallel the expression levels of a plurality of exons or multiexons for each of a plurality of genes in the genome of an organism. The invention further provides methods for determining the effects of perturbations, such as perturbations by drugs, on exon expression and alternative RNA splicing pathways.
摘要翻译：本发明提供了分析细胞或细胞类型的外显子表达谱的方法。在本发明中，对生物体基因组中的多个基因中的每一个的多个单独的外显子或多核苷酸的表达水平进行测定和分析，以确定生物学状态，例如外显子表达状态或转录状态细胞或细胞类型。本发明的方法可用于确定多个基因中的替代RNA剪接。本发明还提供用于平行测定生物体基因组中多个基因中的每一个的多个外显子或多核苷酸的表达水平的核酸探针阵列。本发明还提供了用于确定扰动（例如药物扰动）对外显子表达和替代RNA剪接途径的影响的方法。

5. 发明授权

US07700324B1 Methylated CpG island amplification (MCA) 有权
标题翻译：甲基化CpG岛扩增（MCA）
公开(公告)号：US07700324B1
公开(公告)日：2010-04-20
申请号：US09309175
申请日：1999-05-10
申请人： Jean-Pierre Issa , Stephen Baylin , Minoru Toyota
发明人： Jean-Pierre Issa , Stephen Baylin , Minoru Toyota
IPC分类号： C12P1/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6809 , C12Q1/48 , C12Q1/6855 , C12Q2531/113 , C12Q2525/191 , C12Q2521/331
摘要： The present invention provides a method for identifying a methylated CpG containing nucleic acid by contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated CpG sites and contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, which cleaves both methylated and unmethylated CpG sites. The method also includes amplification of the CpG-containing nucleic acid using CpG-specific oligonucleotide primers A method is also provided for detecting an age associated disorder by identification of a methylated CpG containing nucleic acid. A method is further provided for evaluation the response of a cell to an agent A kit useful for detection of a CpG containing nucleic acid is also provided. Nucleic acid sequences encoding novel methylated clones.
摘要翻译：本发明提供了通过使核酸与切割未甲基化CpG位点的甲基化敏感性限制性内切核酸酶接触并使样品与甲基化敏感性限制性内切核酸酶的异构体接触的方法来鉴定含甲基化CpG的核酸，其将甲基化和未甲基化 CpG位点。该方法还包括使用CpG特异性寡核苷酸引物扩增含CpG的核酸。还提供了通过鉴定含甲基化CpG的核酸来检测年龄相关疾病的方法。还提供了用于评估细胞对试剂的反应的方法。还提供了可用于检测含CpG的核酸的试剂盒。编码新甲基化克隆的核酸序列。

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