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1. 发明授权

US07709232B2 Methods of quantitative detection of genetic recombinants and standard molecules for the methods 有权
标题翻译：方法定量检测遗传重组体和标准分子
公开(公告)号：US07709232B2
公开(公告)日：2010-05-04
申请号：US10423399
申请日：2003-04-25
申请人： Akihiro Hino , Takeshi Matsuoka , Hideo Kuribara , Tomoaki Yoshimura , Yoichiro Shindo , Satoshi Futo , Machiko Ogawa
发明人： Akihiro Hino , Takeshi Matsuoka , Hideo Kuribara , Tomoaki Yoshimura , Yoichiro Shindo , Satoshi Futo , Machiko Ogawa
IPC分类号： C12P1/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6851 , C12N15/8274 , C12N15/8275 , C12N15/8286 , Y02A40/162 , C12Q2525/113 , C12Q2545/101
摘要： The present invention relate to a method of detecting a genetic recombinant by using the PCR method. A method of quantitatively detecting method is provided whereby the total content ratio of the genetic recombinants and the individual content ratio of the genetic recombinant in a population containing plural genetic recombinant lines can be quantified. The method of the present invention comprises performing PCR for the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species corresponding to the recombinant using, as a standard molecule, a molecule containing the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species on the single molecule, and determining the content ratio of the number of molecules thereof.
摘要翻译：本发明涉及使用PCR方法检测遗传重组体的方法。提供了一种定量检测方法，其中可以量化含有多个遗传重组体的群体中遗传重组体的总含量比和遗传重组体的个体含量比。本发明的方法包括对与重组体对应的物种共有的DNA序列进行PCR，所述DNA序列用作重组体和内源性的特异性DNA分子作为标准分子，作为标准分子由单个分子上的物种共享的DNA序列，并确定其分子数目的含量比。

2. 发明授权

US09458491B2 Method and reagent for measuring mevalonic acid, 3-hydroxymethylglutaryl coenzyme A, and coenzyme A 有权
标题翻译：测量甲羟戊酸，3-羟甲基戊二酰辅酶A和辅酶A的方法和试剂
公开(公告)号：US09458491B2
公开(公告)日：2016-10-04
申请号：US13144710
申请日：2010-01-19
申请人： Takeshi Matsuoka
发明人： Takeshi Matsuoka
IPC分类号： C12Q1/26 , C12Q1/48 , C12N9/02 , C12N9/04 , C12N9/12 , C07K1/00 , C07H21/02 , C12Q1/00 , C12R1/01 , C12R1/38 , G01N33/53 , C12N1/20
摘要： The present invention provides a method for measuring the concentration of an analyte in a test solution wherein the analyte is mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A, comprising the following steps (p) and (q): (p) a step of allowing an enzyme that catalyzes a reaction represented by Reaction Formula 1 and an enzyme that catalyzes a reaction represented by Reaction Formula 2 to act on a test solution containing mevalonic acid and/or 3 -hydroxymethylglutaryl coenzyme A in the presence of a hydrogen acceptor X, a hydrogen donor Y, and coenzyme A; and (q) a step of measuring an amount of: a reduced hydrogen acceptor X that is produced; or an oxidized hydrogen donor Y that is produced; or a hydrogen acceptor X that is decreased; or a hydrogen donor Y that is decreased, wherein the hydrogen donor Y and the reduced hydrogen acceptor X are not the same.
摘要翻译：本发明提供了一种用于测量测试溶液中分析物浓度的方法，其中分析物是甲羟戊酸和/或3-羟甲基戊二酰辅酶A，包括以下步骤（p）和（q）：（p）步骤允许催化由反应式1表示的反应的酶和催化由反应式2表示的反应的酶作用于含有甲羟甲酸和/或3-羟甲基戊二酰辅酶A的试验溶液中，在氢受体X的存在下，氢供体Y和辅酶A; 和（q）测量生成的还原氢受体X的量的步骤; 或产生的氧化氢供体Y; 或降低的氢受体X; 或降低的氢供体Y，其中氢供体Y和还原氢受体X不相同。

3. 发明授权

US07344054B2 Power tool 有权
标题翻译：电动工具
公开(公告)号：US07344054B2
公开(公告)日：2008-03-18
申请号：US10973237
申请日：2004-10-27
申请人： Masato Sakai , Takeshi Matsuoka , Chikai Yoshimizu , Masanori Watanabe , Katsuhiro Oomori , Takuya Teranishi
发明人： Masato Sakai , Takeshi Matsuoka , Chikai Yoshimizu , Masanori Watanabe , Katsuhiro Oomori , Takuya Teranishi
IPC分类号： A45F5/00 , B23B39/00 , E21B3/00 , H02J7/00
CPC分类号： B25B23/045 , B25F5/029 , B25H3/006 , Y10S224/904 , Y10T408/96
摘要： A power tool includes a hook portion having a hook holding portion, which is provided in a housing and has engaging teeth provided in the housing. A hook having a shaft portion is inserted into the hook holding portion and provided with fitting teeth meshing with the engaging teeth An elastic body is adapted to push the hook against a handle portion at all times, and a slip-off preventing part holding portion is adapted to move the hook in a direction of an axis thereof against a force of the elastic body and to cancel the mesh between the teeth to thereby enable the hook to turn.
摘要翻译：电动工具包括具有钩保持部分的钩部分，该钩部分设置在壳体中并且具有设置在壳体中的接合齿。具有轴部的钩子被插入到钩保持部分中，并且设置有与接合齿啮合的配合齿。弹性体适于一直将钩推在手柄部分上，并且防脱部分保持部分是适于使钩沿其轴线的方向抵抗弹性体的力移动并抵消齿之间的网孔，从而使钩能够转动。

4. 发明申请

US20070278737A1 PAPER SHEET IDENTIFYING AND CHECKING APPARATUS 审中-公开
标题翻译：纸张识别和检查设备
公开(公告)号：US20070278737A1
公开(公告)日：2007-12-06
申请号：US11755777
申请日：2007-05-31
申请人： Takeshi Matsuoka , Yoshihiro Takada
发明人： Takeshi Matsuoka , Yoshihiro Takada
IPC分类号： B65H7/02
CPC分类号： G07D7/12 , G07D7/17
摘要： In a paper sheet identifying and checking apparatus including a conveying path to convey a bill, a line sensor including a plurality of light sensor elements arranged on the conveying path in a string in a conveying width direction, and an identifying module to identify the paper sheet on the basis of a binary image produced by the line sensor by reading the paper sheet, the direction of arranging the sensor elements is inclined with respect to the conveying width direction of the conveying path.
摘要翻译：在包括用于传送纸币的输送路径的纸张识别和检查装置中，包括布置在输送宽度方向上的串中的输送路径上的多个光传感器元件的线传感器以及识别纸张的识别模块基于通过读取纸张由线传感器产生的二值图像，传感器元件的布置方向相对于输送路径的输送宽度方向倾斜。

5. 外观设计

USD539310S1 Air compressor 有权
公开(公告)号：USD539310S1
公开(公告)日：2007-03-27
申请号：US29254279
申请日：2006-02-22
申请人： Takeshi Matsuoka , Masato Sakai , Mitsuhiro Sunaoshi
设计人： Takeshi Matsuoka , Masato Sakai , Mitsuhiro Sunaoshi

6. 外观设计

USD472780S1 Pneumatically operated portable nail gun 失效
标题翻译：气动手提钉枪
公开(公告)号：USD472780S1
公开(公告)日：2003-04-08
申请号：US29146255
申请日：2001-08-08
申请人： Takeshi Matsuoka , Yoshitaka Akiba
设计人： Takeshi Matsuoka , Yoshitaka Akiba

7. 发明授权

US5577774A Method of recording/reproducing data of mesh pattern, and apparatus therefor 失效
标题翻译：记录/再现网格图案数据的方法及其装置
公开(公告)号：US5577774A
公开(公告)日：1996-11-26
申请号：US196008
申请日：1994-02-10
申请人： Shigenori Morikawa , Takeshi Matsuoka , Hiroshi Chikakiyo
发明人： Shigenori Morikawa , Takeshi Matsuoka , Hiroshi Chikakiyo
IPC分类号： G06K7/14 , G06K19/06 , G10H1/00 , B42D15/00
CPC分类号： G06K7/1417 , G06K19/06037 , G06K7/14 , G10H1/0041 , G06K2019/06253 , G10H2220/445
摘要： A data reading apparatus reads data of dots from a recording sheet on which data is recorded in the form of a mesh pattern, and decodes the read data into binary data. The data reading apparatus has an image sensor to read black/white levels of dots of the mesh pattern using the image sensor and to binarize the read levels by a hardware component or CPU processing according to software. An image can have a high recording density by minimizing a dot size.
摘要翻译：数据读取装置从以网格图案形式记录了数据的记录纸上读取点的数据，并将读取的数据解码为二进制数据。数据读取装置具有使用图像传感器读取网格图案的黑点/黑点的图像传感器，并且根据软件通过硬件部件或CPU处理二值化读取的等级。通过最小化点大小，图像可以具有高记录密度。

8. 发明授权

US08268017B2 Method for stabilizing leuco-type colorant 有权
标题翻译：稳定无色型着色剂的方法
公开(公告)号：US08268017B2
公开(公告)日：2012-09-18
申请号：US12034793
申请日：2008-02-21
申请人： Shigeru Ueda , Takeshi Matsuoka , Akihiko Kan
发明人： Shigeru Ueda , Takeshi Matsuoka , Akihiko Kan
IPC分类号： C09B67/00 , D06P5/00 , G01N33/72 , G03C8/00 , C08B37/16 , C12N9/00 , C12Q1/00 , C12Q1/37 , C12Q1/26
CPC分类号： C12Q1/28 , C09B67/0077
摘要： Provided are a method of stabilizing a leuco dye storable as a liquid for a long time, a method of reducing nonspecific color development thereof at a time of a color development reaction, and a stable liquid reagent composition using the methods. The inventors of the present invention found that coexistence of the leuco dye with a specific reducing agent resulted in suppression of self color development and its remarkably improved stability in a solution, and that when a color development reaction of the leuco dye with hydrogen peroxide was performed, coexistence of the leuco dye, in a reaction solution, with another dye which had an absorption spectrum not influencing a measurement wavelength of the leuco dye and did not react with hydrogen peroxide suppressed nonspecific color development and lowered a reagent blank value, which was applied to an analytical reagent.
摘要翻译：提供一种稳定长时间保存为液体的无色染料的方法，在显色反应时减少其非特异性显色的方法和使用该方法的稳定的液体试剂组合物。本发明人发现，无色染料与特定的还原剂共存导致自色显影的抑制和其在溶液中的显着提高的稳定性，并且当进行无色染料与过氧化氢的显色反应时在无色染料的反应溶液中与另一种不影响无色染料的测定波长的吸收光谱并且不与过氧化氢反应的染料共存抑制非特异性显色，降低试剂空白值分析试剂。

9. 发明申请

US20120040387A1 METHOD AND REAGENT FOR MEASURING MEVALONIC ACID, 3-HYDROXYMETHYLGLUTARYL COENZYME A, AND COENZYME A 审中-公开
标题翻译：用于测量甲酸，3-羟基甲基胆碱酯酶A和COENZYME A的方法和试剂
公开(公告)号：US20120040387A1
公开(公告)日：2012-02-16
申请号：US13144710
申请日：2009-01-19
申请人： Takeshi Matsuoka
发明人： Takeshi Matsuoka
IPC分类号： C12Q1/26 , C07C59/105 , C12N9/04 , C12Q1/48 , C12N1/20
CPC分类号： C12Q1/008 , C12N9/0006 , C12N9/001 , C12N9/1205 , C12Q1/26 , C12R1/01 , C12R1/38 , G01N33/5308 , G01N2333/904
摘要： The present invention provides a method for measuring the concentration of an analyte in a test solution wherein the analyte is mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A, comprising the following steps (p) and (q): (p) a step of allowing an enzyme that catalyzes a reaction represented by Reaction Formula 1 and an enzyme that catalyzes a reaction represented by Reaction Formula 2 to act on a test solution containing mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A in the presence of a hydrogen acceptor X, a hydrogen donor Y, and coenzyme A; and (q) a step of measuring an amount of: a reduced hydrogen acceptor X that is produced; or an oxidized hydrogen donor Y that is produced; or a hydrogen acceptor X that is decreased; or a hydrogen donor Y that is decreased, wherein the hydrogen donor Y and the reduced hydrogen acceptor X are not the same.
摘要翻译：本发明提供了一种用于测量测试溶液中分析物浓度的方法，其中分析物是甲羟戊酸和/或3-羟甲基戊二酰辅酶A，包括以下步骤（p）和（q）：（p）步骤允许催化由反应式1表示的反应的酶和催化反应式2表示的反应的酶在氢受体X存在下作用于含有甲羟戊酸和/或3-羟甲基戊二酰辅酶A的试验溶液，氢供体Y和辅酶A; 和（q）测量生成的还原氢受体X的量的步骤; 或产生的氧化氢供体Y; 或降低的氢受体X; 或降低的氢供体Y，其中氢供体Y和还原氢受体X不相同。

10. 发明授权

US07588910B2 Hemoglobin A1c determination method, enzyme to be used therefor, and production method thereof 有权
标题翻译：血红蛋白A1c测定方法，用于其的酶及其制备方法
公开(公告)号：US07588910B2
公开(公告)日：2009-09-15
申请号：US10557892
申请日：2004-05-21
申请人： Takeshi Matsuoka , Shinji Koga , Takuji Kouzuma , Issei Yoshioka , Atsuhisa Nishimura , Homare Itou , Toshihiko Kumazawa , Takashi Kuroyanagi
发明人： Takeshi Matsuoka , Shinji Koga , Takuji Kouzuma , Issei Yoshioka , Atsuhisa Nishimura , Homare Itou , Toshihiko Kumazawa , Takashi Kuroyanagi
IPC分类号： C12Q1/26 , C12Q1/37
CPC分类号： C12N9/0004 , C12N9/50 , C12Q1/37 , C12R1/01 , C12R1/07 , G01N33/66 , G01N33/723 , G01N2333/95
摘要： There is provided a method for specifically determining a glycated β-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated β-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated α-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated β-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated β-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.
摘要翻译：提供了使用没有分离操作的酶来特异性测定糖化血红蛋白的糖基化β-链N-末端的方法及其测定试剂盒。从糖基化的β-链N-末端切割糖化氨基酸和/或糖基化肽的蛋白酶，不从糖化血红蛋白或其片段的糖基化的α-链N末端基本上切割糖基化的氨基酸或糖化肽被筛选通过使用通过筛选方法获得的蛋白酶来提供糖基化血红蛋白的糖化β-链N-末端的方法和测定试剂盒。根据本发明，糖化血红蛋白的糖化β-链N-末端可以在不进行分离操作的情况下进行确定。

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