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    • 2. 发明申请
    • NOVEL DIGLYCOSIDASE AND GENE ENCODING THE SAME
    • 新型大分子酶和基因编码它们
    • US20100221810A1
    • 2010-09-02
    • US11997005
    • 2006-07-27
    • Kazutaka TsuruhamiShigeharu MoriYoshinao Koide
    • Kazutaka TsuruhamiShigeharu MoriYoshinao Koide
    • C12N9/24C07K14/385C12N15/31C12N15/63C12N1/15
    • C12R1/80C12N9/2402C12P19/12
    • A novel diglycosidase produced by a microorganism belonging to the genus Penicillium, having the following physicochemical properties:(1) action and substrate specificity: it acts on a disaccharide glycoside, releasing the disaccharide sugar and the aglycone thereof; (2) optimum pH: around 4.5; (3) pH stability: it is stable at pH 4.0 to 8.0 under the processing condition of 37° C. for 30 minutes, and retains its 80% or more of the activity even after processing at pH 4.0 or lower; (4) optimum temperature: around 60° C. in a sodium acetate-acetic acid buffer solution (pH 5.5); (5) thermal stability: it is stable at 50° C. or lower in a sodium acetate-acetic acid buffer solution (pH 5.5) and retains 45% of the activity even after processing at 60° C. for 40 minutes; (6) molecular weight: 40,000±5,000 Da based on SDS-PAGE measurement; and (7) isoelectric point (pI): about 4.3.
    • 一种由属于青霉属的微生物产生的新型二糖苷酶,具有以下物理化学性质:(1)作用和底物特异性:其作用于二糖苷,释放二糖和其糖苷配基; (2)最适pH:约4.5; (3)pH稳定性:在37℃的加工条件下在pH4.0〜8.0下稳定30分钟,即使在pH4.0以下处理后仍保持80%以上的活性; (4)最适温度:约60℃在乙酸钠 - 乙酸缓冲溶液(pH5.5)中; (5)热稳定性:在乙酸钠 - 乙酸缓冲溶液(pH5.5)中在50℃以下稳定,即使在60℃下处理40分钟后也保持45%的活性; (6)基于SDS-PAGE测定的分子量:40,000±5,000Da; 和(7)等电点(pI):约4.3。