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1. 发明授权

US07749751B2 Optimized T-DNA transfer and vectors therefor 有权
标题翻译：优化的T-DNA转移和载体
公开(公告)号：US07749751B2
公开(公告)日：2010-07-06
申请号：US10168072
申请日：2000-12-13
申请人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
发明人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
IPC分类号： C12N15/63 , C12N15/09 , C12N15/00
CPC分类号： C12N15/8213 , C12N15/8201 , C12N15/8205 , C12N2800/30 , C12N2810/65
摘要： The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.
摘要翻译：本发明涉及使用所述载体获得转基因真核生物的T-DNA载体和方法。转基因真核生物的特征在于它们含有T-DNA，而不是非法转移的载体骨架序列。这可以通过改变T-DNA边界来实现，使得它们更有效地切割或使得它们允许通过重组消除非法转移的载体骨架序列。

2. 发明授权

US08936907B2 Neural transfection reagents 有权
标题翻译：神经转染试剂
公开(公告)号：US08936907B2
公开(公告)日：2015-01-20
申请号：US13984066
申请日：2012-02-07
申请人： Eric Guire , Jie Wen
发明人： Eric Guire , Jie Wen
IPC分类号： C12Q1/68 , A61K47/32 , C12N15/87 , A61K31/7088 , A61K48/00
CPC分类号： A61K47/32 , A61K31/7088 , A61K48/0075 , C12N15/87 , C12N2810/65
摘要： The invention is directed to transfection reagents for the delivery of nucleic acids into neural cells, compositions including the reagents, methods of preparation of such reagents, methods of transfecting cells with such reagents, and uses thereof. In preferred embodiments the reagents comprise horseradish peroxidase and/or a polycarboxylic acid such as poly(acrylic acid) or poly(methacrylic acid).
摘要翻译：本发明涉及用于将核酸递送到神经细胞的转染试剂，包括试剂的组合物，这种试剂的制备方法，用这种试剂转染细胞的方法及其用途。在优选的实施方案中，试剂包含辣根过氧化物酶和/或多元羧酸，例如聚（丙烯酸）或聚（甲基丙烯酸）。

3. 发明申请

US20070011759A1 Vector mediated organelle transfection 有权
标题翻译：载体介导的细胞器转染
公开(公告)号：US20070011759A1
公开(公告)日：2007-01-11
申请号：US10498328
申请日：2002-12-12
申请人： Shaharyar Khan
发明人： Shaharyar Khan
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C12N5/04
CPC分类号： C07H21/04 , A61K38/44 , A61K48/00 , C12N15/8214 , C12N15/86 , C12N2795/10343 , C12N2800/108 , C12N2810/65 , C12N2810/80
摘要： The present invention provides compositions and methods for direct transfection of mitochondria and chloroplast DNA in living cells. More particularly, the present invention is based on the use of viral vectors that specifically bind to receptors uniquely found on the target organelle. In one embodiment, as shown in FIG. 1, a eukaryotic cell containing an organelle (1) that has been modified to express a viral receptor (2) on the organelle's surface is provided. A viral vector (6) comprising a desired recombinant DNA construct (3) is introduced into the cytosol of the cell, wherein the viral vector binds to its receptor and introduces the recombinant DNA into the interior of the organelle.
摘要翻译：本发明提供了用于在活细胞中直接转染线粒体和叶绿体DNA的组合物和方法。更具体地说，本发明是基于特异性结合目标细胞器上唯一存在的受体的病毒载体的用途。在一个实施例中，如图6所示。提供了含有在细胞器表面上被修饰以表达病毒受体（2）的细胞器（1）的真核细胞。将包含所需重组DNA构建体（3）的病毒载体（6）引入细胞的胞质溶胶中，其中病毒载体与其受体结合并将重组DNA引入细胞器的内部。

4. 发明申请

US20080047035A9 Optimized t-dna transfer and vectors therefor 有权
标题翻译：优化t-dna转移和载体
公开(公告)号：US20080047035A9
公开(公告)日：2008-02-21
申请号：US10168072
申请日：2000-12-13
申请人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
发明人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
IPC分类号： A01H1/00 , C12N15/74 , C12N15/82 , C12N1/16 , C12N1/18 , C12N1/21
CPC分类号： C12N15/8213 , C12N15/8201 , C12N15/8205 , C12N2800/30 , C12N2810/65
摘要： The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.
摘要翻译：本发明涉及使用所述载体获得转基因真核生物的T-DNA载体和方法。转基因真核生物的特征在于它们含有T-DNA，而不是非法转移的载体骨架序列。这可以通过改变T-DNA边界来实现，使得它们更有效地切割或使得它们允许通过重组消除非法转移的载体骨架序列。

5. 发明申请

US20030140376A1 Optimized t-dna transfer and vectors therefor 有权
标题翻译：优化t-dna转移和载体
公开(公告)号：US20030140376A1
公开(公告)日：2003-07-24
申请号：US10168072
申请日：2002-10-29
发明人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
IPC分类号： A01H001/00 , C12N015/74 , C12N015/82 , C12N001/16 , C12N001/18 , C12N001/21
CPC分类号： C12N15/8213 , C12N15/8201 , C12N15/8205 , C12N2800/30 , C12N2810/65
摘要： The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.
摘要翻译：本发明涉及使用所述载体获得转基因真核生物的T-DNA载体和方法。转基因真核生物的特征在于它们含有T-DNA，而不是非法转移的载体骨架序列。这可以通过改变T-DNA边界来实现，使得它们更有效地切割或使得它们允许通过重组消除非法转移的载体骨架序列。

6. 发明授权

US5529909A Tyrosinase-activator protein fusion enzyme 失效
标题翻译：酪氨酸酶激活蛋白融合酶
公开(公告)号：US5529909A
公开(公告)日：1996-06-25
申请号：US152483
申请日：1993-11-12
申请人： Guy della-Cioppa , Monto Kumagai
发明人： Guy della-Cioppa , Monto Kumagai
IPC分类号： A01H5/00 , C07K14/08 , C07K14/095 , C07K14/36 , C07K14/415 , C07K14/47 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/24 , C12N9/72 , C12N9/78 , C12N9/84 , C12N15/09 , C12N15/12 , C12N15/53 , C12N15/82 , C12N15/86 , C12P17/00 , C12P17/18 , C12P41/00 , C12R1/19 , C12R1/48 , C12N1/20 , C12N15/62
CPC分类号： C12N9/0071 , C07K14/36 , C07K14/415 , C07K14/4705 , C12N15/8289 , C12N15/86 , C12N9/0059 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12P17/00 , C12P41/003 , C12Y114/18001 , C12Y302/01031 , C12Y304/21069 , C07K2319/00 , C12N2795/14143 , C12N2810/65
摘要： The present invention relates to a nucleic acid sequence that encodes a fusion enzyme of tyrosinase and a tyrosinase activator protein. Further, the present invention also relates to the amino acid sequence which is encoded by the nucleic acid sequence of the fusion enzyme. The fusion enzyme may also include a linker positioned between the amino acid sequences of the tyrosinase and the tyrosinase activator protein. Still further, the present invention also relates to a vector useful for introducing the nucleic acid sequence encoding the fusion enzyme into an organism. Still further, the present invention relates to melanins made by the fusion enzyme and a method for making melanins using the fusion enzyme.
摘要翻译：本发明涉及编码酪氨酸酶和酪氨酸酶活化蛋白的融合酶的核酸序列。此外，本发明还涉及由融合酶的核酸序列编码的氨基酸序列。融合酶还可以包括位于酪氨酸酶和酪氨酸酶活化蛋白的氨基酸序列之间的连接体。此外，本发明还涉及可用于将编码融合酶的核酸序列引入生物体的载体。此外，本发明涉及由融合酶制成的黑色素和使用该融合酶制备黑色素的方法。

7. 发明申请

US20130317086A1 NEURAL TRANSFECTION REAGENTS 有权
标题翻译：神经转移试剂
公开(公告)号：US20130317086A1
公开(公告)日：2013-11-28
申请号：US13984066
申请日：2012-02-07
申请人： Eric Guire , Jie Wen
发明人： Eric Guire , Jie Wen
IPC分类号： A61K47/32 , A61K31/7088
CPC分类号： A61K47/32 , A61K31/7088 , A61K48/0075 , C12N15/87 , C12N2810/65
摘要： The invention is directed to transfection reagents for the delivery of nucleic acids into neural cells, compositions including the reagents, methods of preparation of such reagents, methods of transfecting cells with such reagents, and uses thereof. In preferred embodiments the reagents comprise horseradish peroxidase and/or a polycarboxylic acid such as poly(acrylic acid) or poly(methacrylic acid).
摘要翻译：本发明涉及用于将核酸递送到神经细胞的转染试剂，包括试剂的组合物，这种试剂的制备方法，用这种试剂转染细胞的方法及其用途。在优选的实施方案中，试剂包含辣根过氧化物酶和/或多元羧酸，例如聚（丙烯酸）或聚（甲基丙烯酸）。

8. 发明申请

US20100229260A1 OPTIMIZED T-DNA TRANSFER AND VECTORS THEREFOR 审中-公开
标题翻译：优化的T-DNA转移和载体
公开(公告)号：US20100229260A1
公开(公告)日：2010-09-09
申请号：US12783059
申请日：2010-05-19
申请人： ANNA DEPICKER , VLADIMIR MIRONOV , FRANKY TERRAS , WILLEM BROEKAERT , SYLVIE DE BUCK , CHRIS DE WILDE
发明人： ANNA DEPICKER , VLADIMIR MIRONOV , FRANKY TERRAS , WILLEM BROEKAERT , SYLVIE DE BUCK , CHRIS DE WILDE
IPC分类号： C12N15/74 , C12N15/63 , C12N15/82 , C12N1/21 , C12N5/10 , C12N1/19 , C12N1/15 , A01H5/00
CPC分类号： C12N15/8213 , C12N15/8201 , C12N15/8205 , C12N2800/30 , C12N2810/65
摘要： The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.
摘要翻译：本发明涉及使用所述载体获得转基因真核生物的T-DNA载体和方法。转基因真核生物的特征在于它们含有T-DNA，而不是非法转移的载体骨架序列。这可以通过改变T-DNA边界来实现，使得它们更有效地切割或使得它们允许通过重组消除非法转移的载体骨架序列。

9. 发明授权

US07741112B2 Vector mediated organelle transfection 有权
标题翻译：载体介导的细胞器转染
公开(公告)号：US07741112B2
公开(公告)日：2010-06-22
申请号：US10498328
申请日：2002-12-12
申请人： Shaharyar M. Khan
发明人： Shaharyar M. Khan
IPC分类号： C12N15/63 , C12N5/00
CPC分类号： C07H21/04 , A61K38/44 , A61K48/00 , C12N15/8214 , C12N15/86 , C12N2795/10343 , C12N2800/108 , C12N2810/65 , C12N2810/80
摘要： The present invention provides compositions and methods for direct transfection of mitochondria and chloroplast DNA in living cells. More particularly, the present invention is based on the use of viral vectors that specifically bind to receptors uniquely found on the target organelle. In one embodiment, as shown in FIG. 1, a eukaryotic cell containing an organelle (1) that has been modified to express a viral receptor (2) on the organelle's surface is provided. A viral vector (6) comprising a desired recombinant DNA construct (3) is introduced into the cytosol of the cell, wherein the viral vector binds to its receptor and introduces the recombinant DNA into the interior of the organelle.
摘要翻译：本发明提供了用于在活细胞中直接转染线粒体和叶绿体DNA的组合物和方法。更具体地说，本发明是基于特异性结合目标细胞器上唯一存在的受体的病毒载体的用途。在一个实施例中，如图1所示。 1，提供了含有在细胞器表面上被修饰以表达病毒受体（2）的细胞器（1）的真核细胞。将包含所需重组DNA构建体（3）的病毒载体（6）引入细胞的胞质溶胶中，其中病毒载体与其受体结合并将重组DNA引入细胞器的内部。

10. 发明授权

US06700038B1 Plant expression vectors based on the flock house virus genome 失效
标题翻译：基于鸡群病毒基因组的植物表达载体
公开(公告)号：US06700038B1
公开(公告)日：2004-03-02
申请号：US09281851
申请日：1999-03-31
申请人： Ranjit K. Dasgputa , Robert Goodman
发明人： Ranjit K. Dasgputa , Robert Goodman
IPC分类号： C12N1582
CPC分类号： C12N15/8203 , C12N15/86 , C12N2770/30043 , C12N2810/65
摘要： The invention provides insect viral vectors useful to transfer genes to plants, insects and other hosts.
摘要翻译：本发明提供了可用于将基因转移至植物，昆虫和其他宿主的昆虫病毒载体。

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