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1. 发明授权

US07749751B2 Optimized T-DNA transfer and vectors therefor 有权
标题翻译：优化的T-DNA转移和载体
公开(公告)号：US07749751B2
公开(公告)日：2010-07-06
申请号：US10168072
申请日：2000-12-13
申请人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
发明人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
IPC分类号： C12N15/63 , C12N15/09 , C12N15/00
CPC分类号： C12N15/8213 , C12N15/8201 , C12N15/8205 , C12N2800/30 , C12N2810/65
摘要： The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.
摘要翻译：本发明涉及使用所述载体获得转基因真核生物的T-DNA载体和方法。转基因真核生物的特征在于它们含有T-DNA，而不是非法转移的载体骨架序列。这可以通过改变T-DNA边界来实现，使得它们更有效地切割或使得它们允许通过重组消除非法转移的载体骨架序列。

2. 发明申请

US20080047035A9 Optimized t-dna transfer and vectors therefor 有权
标题翻译：优化t-dna转移和载体
公开(公告)号：US20080047035A9
公开(公告)日：2008-02-21
申请号：US10168072
申请日：2000-12-13
申请人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
发明人： Anna Depicker , Vladimir Mironov , Franky Terras , Willem Broekaert , Sylvie De Buck , Chris De Wilde
IPC分类号： A01H1/00 , C12N15/74 , C12N15/82 , C12N1/16 , C12N1/18 , C12N1/21
CPC分类号： C12N15/8213 , C12N15/8201 , C12N15/8205 , C12N2800/30 , C12N2810/65
摘要： The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.
摘要翻译：本发明涉及使用所述载体获得转基因真核生物的T-DNA载体和方法。转基因真核生物的特征在于它们含有T-DNA，而不是非法转移的载体骨架序列。这可以通过改变T-DNA边界来实现，使得它们更有效地切割或使得它们允许通过重组消除非法转移的载体骨架序列。

3. 发明授权

US07592507B2 Method to modify cell number, architecture and yield of plants by overexpressing the E2F transcription factor 失效
标题翻译：通过过表达E2F转录因子修饰植物的细胞数量，结构和产量的方法
公开(公告)号：US07592507B2
公开(公告)日：2009-09-22
申请号：US10489500
申请日：2002-09-12
申请人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
发明人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译：本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。更具体地，本发明涉及刺激植物细胞分裂的方法。本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。

4. 发明申请

US20070199085A1 Plants Having Modified Growth Characteristics And Method For Making Same 审中-公开
标题翻译：具有改良生长特性的植物及其制作方法
公开(公告)号：US20070199085A1
公开(公告)日：2007-08-23
申请号：US10570554
申请日：2004-09-03
申请人： Willem Broekaert , Valerie Frankard , Yves Hatzfeld , Vladimir Mironov
发明人： Willem Broekaert , Valerie Frankard , Yves Hatzfeld , Vladimir Mironov
IPC分类号： A01H1/00 , C07H21/04 , C12N9/12 , C12N15/82 , C12N5/04
CPC分类号： C12N9/1205 , C12N15/8262 , C12Q1/485 , G01N2333/39 , G01N2500/10
摘要： The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a B-type CDK nucleic acid and/or modulating activity and/or levels in a plant of a B-type CDK protein. The invention also relates to transgenic plants having modified growth characteristics, which plants have modulated expression of a B-type CDK nucleic acid and/or modulated activity and/or levels in a plant of a B-type CDK protein relative to expression, activity and/or levels in corresponding wild-type plants. The present invention also provides a novel screening method for the identification of mutant CDKs having enhanced CDK activity relative to corresponding non-mutated CDKs. The present invention also provides a novel screening method for the identification of non-active CDKs that are able to bind to CKIs (cyclin dependent kinase inhibitors). The invention also provides mutant CDKs obtainable by the screening methods according to the invention.
摘要翻译：本发明涉及通过调节B型CDK核酸的植物表达和/或调节B型CDK蛋白的植物中的活性和/或水平来修饰植物生长特性的方法。本发明还涉及具有修饰的生长特性的转基因植物，相对于表达，活性，植物调节B型CDK核酸的表达和/或调节的活性和/或B型CDK蛋白的植物中的水平 /或相应野生型植物中的水平。本发明还提供了一种用于鉴定相对于相应的非突变CDKs具有增强的CDK活性的突变CDK的新型筛选方法。本发明还提供了用于鉴定能够结合CKI（细胞周期蛋白依赖性激酶抑制剂）的非活性CDK的新型筛选方法。本发明还提供可通过本发明的筛选方法获得的突变体CDK。

5. 发明申请

US20050059154A1 Method to modify cell number, architecture and yield of plants by overexpressing the e2f transcription factor 失效
标题翻译：通过过表达e2f转录因子来修饰植物的细胞数量，结构和产量的方法
公开(公告)号：US20050059154A1
公开(公告)日：2005-03-17
申请号：US10489500
申请日：2002-09-12
申请人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
发明人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
IPC分类号： A01H5/00 , C12N15/82 , A01H1/00
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译：本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。更具体地，本发明涉及刺激植物细胞分裂的方法。本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。

6. 发明授权

US08741376B2 Nutriment containing arabinoxylan and oligosaccharides 有权
标题翻译：含阿拉伯木聚糖和寡糖的营养成分
公开(公告)号：US08741376B2
公开(公告)日：2014-06-03
申请号：US13139050
申请日：2009-12-10
申请人： Willem Broekaert , Christophe Courtin , Bram Damen , Jan Delcour
发明人： Willem Broekaert , Christophe Courtin , Bram Damen , Jan Delcour
IPC分类号： A23G3/00
CPC分类号： A23L2/02 , A21D2/181 , A21D2/36 , A23L7/109 , A23L7/115 , A23L7/122 , A23L33/22
摘要： The present invention provides nutritional compositions, more particularly food supplements and processed food products, enriched with arabinoxylan-oligosaccharides and further comprising either or both water-unextractable arabinoxylans or water-soluble arabinoxylans. Preferably said nutritional compositions comprises both arabinoxylan-oligosaccharides and water-unextractable arabinoxylans.
摘要翻译：本发明提供富含阿拉伯木聚寡糖的营养组合物，更特别是食品添加剂和加工食品，并且还包含水不可提取的阿拉伯木聚糖或水溶性阿拉伯木聚糖中的一种或两种。优选地，所述营养组合物包含阿拉伯木聚寡糖和水不可提取的阿拉伯木聚糖。

7. 发明申请

US20120096591A1 Identification and Validation of Novel Targets for Agrochemicals 审中-公开
标题翻译：鉴定和验证农药新品目标
公开(公告)号：US20120096591A1
公开(公告)日：2012-04-19
申请号：US13210461
申请日：2011-08-16
申请人： Dirk Inze , Willem Broekaert
发明人： Dirk Inze , Willem Broekaert
IPC分类号： A01H1/06 , A01H5/00 , C12N15/12
CPC分类号： C12N15/8261 , C12N15/8274 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , Y02A40/146
摘要： The invention relates to a method for identifying and validating plant targets for agrochemicals, comprising the steps of determining gene or protein expression profiles in function of the progression of an essential biological process in a plant subsequent downregulation of expression of said gene or protein in a plant cell. More particularly, the effects of downregulation of the candidate target gene were directly monitored on plants locally infected with a vector mediating viral induced gene suppression in that infected plant area. The invention also relates to isolated plant genes encoding proteins involved in plant growth and development. The invention also relates to plants tolerant to agrochemicals such as herbicides or pesticides.
摘要翻译：本发明涉及用于鉴定和验证农用化学品植物靶标的方法，包括以下步骤：确定基因或蛋白质表达谱，其功能是植物中必需生物过程的进展，随后下调基因或蛋白质在植物中的表达细胞。更具体地说，候选靶基因的下调效应直接由在感染的植物区域中介导病毒诱导的基因抑制的载体局部感染的植物上进行监测。本发明还涉及编码参与植物生长和发育的蛋白质的分离的植物基因。本发明还涉及耐农业化学品如除草剂或杀虫剂的植物。

8. 发明申请

US20120015902A1 ARABINOXYLO-OLIGOSACCHARIDES USEFUL AGAINST GASTROINTESTINAL INFECTIONS 有权
标题翻译：有利于胃肠道感染的阿氨苄氧低聚糖
公开(公告)号：US20120015902A1
公开(公告)日：2012-01-19
申请号：US12680435
申请日：2008-09-29
申请人： Willem Broekaert , Jan Delcour
发明人： Willem Broekaert , Jan Delcour
IPC分类号： A61K31/715 , A61P31/00 , A61P1/00 , C08B37/00
CPC分类号： A61K31/716 , A23L7/115 , A23L33/10 , A23L33/21 , A61K31/00 , A61K31/715
摘要： The present invention relates to the use of oligosaccharides derived from arabinoxylan for use in the prevention and treatment of gastrointestinal infection. More particularly the invention provides a method for preventing or reducing the gastrointestinal infection of a animal or human being with bacteria associated with gastroenteritis through the supplementation of their diets with the said oligosaccharides.
摘要翻译：本发明涉及来自阿拉伯木聚糖的寡糖用于预防和治疗胃肠道感染的用途。更具体地，本发明提供了通过用所述寡糖补充其饮食来预防或减少与胃肠炎相关的细菌的动物或人的胃肠道感染的方法。

9. 发明申请

US20110020498A1 (ARABINO)XYLAN OLIGOSACCHARIDE PREPARATION 有权
标题翻译：（阿拉伯）XYLAN低聚糖制剂
公开(公告)号：US20110020498A1
公开(公告)日：2011-01-27
申请号：US12934224
申请日：2009-03-25
申请人： Willem Broekaert , Christophe Courtin , Jan Delcour
发明人： Willem Broekaert , Christophe Courtin , Jan Delcour
IPC分类号： C08B30/20 , A23G3/00
CPC分类号： A23L1/3002 , A21D2/18 , A21D2/183 , A23K20/163 , A23K50/40 , A23L2/52 , A23L33/105 , A23V2002/00 , A23V2200/3202 , A23V2250/638 , A23V2250/602
摘要： The present invention relates to preparations comprising (arabino)xylan oligosaccharides with average degree of polymerisation between 4 and 10 and average degree of arabinose substitution between 0.15 and 0.35, which beneficially modulate the intestinal flora, such preparation comprising both xylo- and arabinoxylo-oligosaccharides. Preferably, the arabinoxylooligosaccharides are substituted in part by hydroxycinnamic acids and exert antioxidant effects. Furthermore, it is preferred that the preparations according to the present invention have a nitrogen content below 0.16% (w/w) in order to ensure good organoleptic and colour properties, particularly upon heating said preparations. In addition, food, beverage and nutritional supplement products comprising the preparations are provided as well as methods to prepare the said preparations.
摘要翻译：本发明涉及包含（阿拉伯糖）木聚糖寡糖的制剂，其平均聚合度为4至10，平均阿拉伯糖取代度为0.15至0.35，其有利地调节肠道菌群，所述制剂包含木糖和阿拉伯糖基寡糖。优选地，所述阿拉伯寡脱氧木糖部分被羟基肉桂酸取代并发挥抗氧化作用。此外，为了确保良好的感官和颜色特性，特别是在加热所述制剂时，优选本发明的制剂具有低于0.16％（w / w）的氮含量。此外，提供包含制剂的食品，饮料和营养补充剂产品以及制备所述制剂的方法。

10. 发明申请

US20100040731A1 ARABINOXYLO-OLIGOSACCHARIDES IN BEER 审中-公开
标题翻译：啤酒中的阿拉伯糖 - 低聚糖
公开(公告)号：US20100040731A1
公开(公告)日：2010-02-18
申请号：US12541631
申请日：2009-08-14
申请人： Guido Aerts , Willem Broekaert , Christophe Courtin , Jan Delcour
发明人： Guido Aerts , Willem Broekaert , Christophe Courtin , Jan Delcour
IPC分类号： C12G3/08
CPC分类号： C12C5/004 , C12C12/02 , C12C12/04
摘要： This invention provides beers with increased amounts of soluble arabinoxylans having an average degree of polymerisation (DP) below 50 for improving or significantly altering their mouthfeel. This invention also provides methods for enriching a wort or beer with such soluble arabinoxylans during the brewing process.
摘要翻译：本发明提供了具有平均聚合度（DP）低于50的可溶性阿拉伯木聚糖的量增加的啤酒，以改善或显着改变其口感。本发明还提供了在酿造过程中用这种可溶性阿拉伯木聚糖富集麦汁或啤酒的方法。

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