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1. 发明申请

US20020100066A1 NEGATIVE STRAND RNA VIRAL VECTOR HAVING AUTONOMOUS REPLICATION CAPABILITY 失效
标题翻译：具有自主复制能力的负反馈RNA病毒载体
公开(公告)号：US20020100066A1
公开(公告)日：2002-07-25
申请号：US09070938
申请日：1998-04-30
发明人： YOSHIYUKI NAGAI , ATSUSHI KATO , FUKASHI MURAI , MAKOTO ASAKAWA , TSUNEAKI SAKATA , MAMORU HASEGAWA , TATSUO SHIODA
IPC分类号： A01K067/00 , C07H021/04 , C12P021/02 , C12N005/06 , C12N015/86
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2740/16122 , C12N2760/18821 , C12N2760/18822 , C12N2760/18843 , C12N2760/18852
摘要： A process for reconstituting virions of Sendai virus by introducing Sendai virus into a host in which early replication genes have been all expressed. This process makes it possible to produce a (null)-strand RNA virus vector with a high practical value.
摘要翻译：通过将仙台病毒引入到早期复制基因全部被表达的宿主中来重建仙台病毒的病毒粒子的过程。该方法使得可以生产具有高实用价值的（ - ） - 链RNA病毒载体。

2. 发明授权

US06723532B2 Negative strand RNA viral vector having autonomous replication capability 失效
标题翻译：具有自主复制能力的负链RNA病毒载体
公开(公告)号：US06723532B2
公开(公告)日：2004-04-20
申请号：US09070938
申请日：1998-04-30
申请人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Makoto Asakawa , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
发明人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Makoto Asakawa , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
IPC分类号： C12N1500
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2740/16122 , C12N2760/18821 , C12N2760/18822 , C12N2760/18843 , C12N2760/18852
摘要： A method for reconstituting Sendai viral particles by transfecting Sendai virus to a host expressing all genes for the initial replication has been developed, enabling the Production of negative strand RNA vectors highly useful for practical applications.
摘要翻译：已经开发了通过将仙台病毒转染到表达用于初始复制的所有基因的宿主来重建仙台病毒颗粒的方法，使得能够生产对实际应用非常有用的负链RNA载体。

3. 发明申请

US20030022376A1 Paramyxovirus-derived RNP 审中-公开
标题翻译：副粘病毒衍生的RNP
公开(公告)号：US20030022376A1
公开(公告)日：2003-01-30
申请号：US09966930
申请日：2001-09-27
发明人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Takahiro Hirata , Makoto Inoue
IPC分类号： C12N015/86 , C12N007/00
CPC分类号： C12N15/86 , C12N15/87 , C12N2760/18843 , C12N2760/18851 , C12N2760/18852 , C12N2760/18862 , C12N2810/6081
摘要： A functional RNP containing negative-strand single-stranded RNA derived from Sendai virus, which has been modified so as not to express any envelope protein, has been successfully prepared. An RNP comprising a foreign gene is prepared and inserted into a cell with the use of a cationic liposome, thereby successfully expressing the foreign gene.
摘要翻译：已经成功地制备了一种功能性的RNP，其具有来自仙台病毒的负链单链RNA，其被修饰以便不表达任何包膜蛋白。制备包含外来基因的RNP，并使用阳离子脂质体将其插入细胞中，从而成功地表达外源基因。

4. 发明公开

US20240309335A1 METHOD OF PRODUCING NEGATIVE-STRAND RNA VIRUS VECTOR AND PRODUCED NEGATIVE-STRAND RNA VIRUS VECTOR 审中-公开
公开(公告)号：US20240309335A1
公开(公告)日：2024-09-19
申请号：US18575633
申请日：2022-06-29
申请人： REPLI-TECH CO., LTD.
发明人： Koichi SAEKI
IPC分类号： C12N7/00 , C12N5/071 , C12N15/86
CPC分类号： C12N7/00 , C12N5/0686 , C12N15/86 , C12N2710/24122 , C12N2720/12022 , C12N2760/16122 , C12N2760/18421 , C12N2760/18452 , C12N2760/18721 , C12N2760/18752 , C12N2760/18821 , C12N2760/18843 , C12N2760/18852 , C12N2760/20221 , C12N2760/20252
摘要： The present disclosure provides a method of producing a negative-strand RNA virus vector. The present disclosure specifically provides a method of producing a negative-strand RNA virus vector in the presence of a PKR inhibitory factor.

5. 发明授权

US09951349B2 Compositions and methods for transient expression of recombinant RNA 有权
公开(公告)号：US09951349B2
公开(公告)日：2018-04-24
申请号：US14343817
申请日：2012-09-27
申请人： Yale University
发明人： Peter M. Rabinovich , Sherman M. Weissman
IPC分类号： C12N15/85 , C12N15/86
CPC分类号： C12N15/85 , C12N15/86 , C12N2760/18843 , C12N2760/18852
摘要： Compositions for transient but prolonged exogenous mRNA expression through the use of the transcription system of negative strand RNA viruses, and methods of use thereof are disclosed. In some embodiments, the system contains only RNAs and does not include any DNA molecules. The compositions typically include an RNA template unit (rTeUn) that includes a virus regulatory sequences operably linked to a coding sequence of interest. The rTeUn is typically transfected to a host cell's cytoplasm in the presence of virus expression system proteins that mediate replication of the rTeUn and transcription of the transgene. The rTeUn RNA bonded to viral proteins exhibits high resistance to degradation, prolonged duration of expression, and is free of viral genes. The compositions can be used to reprogram cell. For example, the compositions and methods can be used to redirected lymphocytes to target cancer cells, or to dedifferentiate somatic cells into induce pluripotent stem cells.

6. 发明申请

US20140249212A1 Compositions and Methods for Transient Expression of Recombinant RNA 有权
标题翻译：用于重组RNA瞬时表达的组合物和方法
公开(公告)号：US20140249212A1
公开(公告)日：2014-09-04
申请号：US14343817
申请日：2012-09-27
申请人： Yale University
发明人： Peter M. Rabinovich , Sherman M. Weissman
IPC分类号： C12N15/85
CPC分类号： C12N15/85 , C12N15/86 , C12N2760/18843 , C12N2760/18852
摘要： Compositions for transient but prolonged exogenous mRNA expression through the use of the transcription system of negative strand RNA viruses, and methods of use thereof are disclosed. In some embodiments, the system contains only RNAs and does not include any DNA molecules. The compositions typically include an RNA template unit (rTeUn) that includes a virus regulatory sequences operably linked to a coding sequence of interest. The rTeUn is typically transfected to a host cell's cytoplasm in the presence of virus expression system proteins that mediate replication of the rTeUn and transcription of the transgene. The rTeUn RNA bonded to viral proteins exhibits high resistance to degradation, prolonged duration of expression, and is free of viral genes. The compositions can be used to reprogram cell. For example, the compositions and methods can be used to redirected lymphocytes to target cancer cells, or to dedifferentiate somatic cells into induce pluripotent stem cells.
摘要翻译：公开了通过使用负链RNA病毒的转录系统的瞬时但延长的外源mRNA表达的组合物及其使用方法。在一些实施方案中，系统仅含有RNA并且不包括任何DNA分子。组合物通常包括RNA模板单元（rTeUn），其包含与感兴趣的编码序列可操作地连接的病毒调节序列。在介导rTeUn复制和转录转录的病毒表达系统蛋白的存在下，rTeUn通常被转染至宿主细胞的细胞质。与病毒蛋白结合的rTeUn RNA表现出高度的降解抗性，延长的表达持续时间，并且没有病毒基因。组合物可用于重新编程细胞。例如，组合物和方法可用于将淋巴细胞重定向至靶癌细胞，或将体细胞去分化为诱导多能干细胞。

7. 发明申请

US20050266566A1 Recombinant sendai virus 有权
标题翻译：重组发甲病毒
公开(公告)号：US20050266566A1
公开(公告)日：2005-12-01
申请号：US11130117
申请日：2005-05-17
申请人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
发明人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
IPC分类号： C07H21/02 , C07K14/115 , C12N5/10 , C12N7/00 , C12N7/01 , C12N15/86
CPC分类号： C07K14/005 , C12N7/00 , C12N15/86 , C12N2760/18843 , C12N2760/18852
摘要： A method for regenerating Sendai virus particles by transfecting the Sendai virus genome to a host expressing all genes for the initial viral replication has been developed, enabling the genetic manipulation of Sendai virus and effective utilization of said virus as the vector.
摘要翻译：已经开发了通过将仙台病毒基因组转染到表达用于初始病毒复制的所有基因的宿主来再生仙台病毒颗粒的方法，使得能够对仙台病毒进行遗传操作并有效利用所述病毒作为载体。

8. 发明授权

US06645760B2 Negative strand RNA viral vector having autonomous replication capability 有权
标题翻译：具有自主复制能力的负链RNA病毒载体
公开(公告)号：US06645760B2
公开(公告)日：2003-11-11
申请号：US09471840
申请日：1999-12-23
申请人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Makoto Asakawa , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
发明人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Makoto Asakawa , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
IPC分类号： C12N500
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2740/16122 , C12N2760/18821 , C12N2760/18822 , C12N2760/18843 , C12N2760/18852
摘要： A method for reconstituting Sendai viral particles by transfecting Sendai virus to a host expressing all genes for the initial replication has been developed, enabling the production of negative strand RNA vectors highly useful for practical applications.
摘要翻译：已经开发了通过将仙台病毒转染到表达用于初始复制的所有基因的宿主来重建仙台病毒颗粒的方法，使得能够生产对实际应用非常有用的负链RNA载体。

9. 发明申请

US20020098576A1 Recombinant sendai virus 有权
标题翻译：重组发甲病毒
公开(公告)号：US20020098576A1
公开(公告)日：2002-07-25
申请号：US09728207
申请日：2000-12-01
发明人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
IPC分类号： C12N007/00 , C12N007/01 , C07H021/04 , A61K039/155 , C12N015/09 , C12N015/70 , A01N063/00 , C12N015/00 , C12N015/63 , C12N015/74
CPC分类号： C07K14/005 , C12N7/00 , C12N15/86 , C12N2760/18843 , C12N2760/18852
摘要： A method for regenerating Sendai virus particles by transfecting the Sendai virus genome to a host expressing all genes for the initial viral replication has been developed, enabling the genetic manipulation of Sendai virus and effective utilization of said virus as the vector.
摘要翻译：已经开发了通过将仙台病毒基因组转染到表达用于初始病毒复制的所有基因的宿主来再生仙台病毒颗粒的方法，使得能够对仙台病毒进行遗传操作并有效利用所述病毒作为载体。

10. 发明申请

US20150133531A1 METHOD FOR EXPRESSION OF HETEROLOGOUS PROTEINS USING A RECOMBINANT NEGATIVE-STRAND RNA VIRUS VECTOR 审中-公开
标题翻译：使用重组负反馈RNA病毒载体表达异位蛋白的方法
公开(公告)号：US20150133531A1
公开(公告)日：2015-05-14
申请号：US14404109
申请日：2013-05-24
申请人： AmVac AG
发明人： Marian Wiegand
IPC分类号： C12N15/86 , C07K14/47 , C12N7/00
CPC分类号： C12N15/86 , A61K48/00 , C07K14/4702 , C12N5/0696 , C12N7/00 , C12N2760/18822 , C12N2760/18843 , C12N2760/18852 , C12N2760/18862 , C12N2800/24
摘要： The present invention provides a method of expressing at least one heterologous nucleic acid sequence in a cell, the method comprising introducing at least one heterologous nucleic acid sequence into a cell by infecting said cell with a recombinant negative-strand RNA virus vector comprising said at least one heterologous nucleic acid sequence, wherein the recombinant negative-strand RNA virus vector includes a viral genome coding for a mutated P protein, which leads to a loss of the viral genome replication ability without a loss of the viral transcription ability, and wherein said at least one heterologous nucleic acid sequence encodes a cellular reprogramming or programming factor or a therapeutic protein. In addition, the present invention provides a cell or a population of cells prepared in vitro by said method as well as a pharmaceutical composition comprising said cell or population of cells.
摘要翻译：本发明提供了在细胞中表达至少一种异源核酸序列的方法，所述方法包括通过用重组负链RNA病毒载体感染所述细胞将至少一种异源核酸序列引入细胞，所述重组负链RNA病毒载体包含至少一种异源核酸序列，其中重组负链RNA病毒载体包括编码突变的P蛋白的病毒基因组，其导致病毒基因组复制能力的丧失而不损失病毒转录能力，并且其中所述至少一个异源核酸序列编码细胞重编程或编程因子或治疗性蛋白质。此外，本发明提供了通过所述方法体外制备的细胞或细胞群以及包含所述细胞或细胞群的药物组合物。

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