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1. 发明公开

US20240156944A1 ATTENUATED REOVIRUS-BASED VACCINE COMPOSITION AND USE THEREOF 审中-公开
公开(公告)号：US20240156944A1
公开(公告)日：2024-05-16
申请号：US18283363
申请日：2022-03-22
申请人： VIROCURE, INC.
发明人： Haeng Jun YOO , Tommy Michel ALAIN , Sang Kyoung HAN , Xiao XIANG , So Young KIM , Yeon Sook LEE , Ki Hoon SONG
IPC分类号： A61K39/15 , A61K39/00 , A61P37/04 , C07K14/005
CPC分类号： A61K39/15 , A61P37/04 , C07K14/005 , A61K2039/5254 , C12N2720/12022 , C12N2720/12034
摘要： The present invention relates to an attenuated reovirus-based vaccine composition and a use thereof, the attenuated reovirus, according to the present invention, having the 251st to 455th amino acids of a sigma-1 protein of a capsid truncated such that when an epitope of an antigenic protein inducing cancer or infectious disease is introduced to the truncated site of the sigma-1 protein, the epitope of the antigenic protein is stably expressed in a cell, and thus the effect is gained of exhibiting an immune response such as producing a neutralizing antibody or inducing cell-mediated immunity. As such, the present invention is expected to be usefully employable as a vaccine composition for cancer or infectious disease by introducing the epitope of the antigenic protein to the truncated site of the sigma-1 protein of the attenuated reovirus according to the present invention.

2. 发明申请

US20160287694A1 PISCINE REOVIRUS IMMUNOGENIC COMPOSITIONS 审中-公开
标题翻译： PISCINE REOVIRUS免疫组化
公开(公告)号：US20160287694A1
公开(公告)日：2016-10-06
申请号：US15181916
申请日：2016-06-14
申请人： The Trustees of Columbia University in the City of New York , The National Veterinary Institute
发明人： W. Ian Lipkin , Gustavo Palacios , Ruth Toril Kongtorp , Edgar Brun
IPC分类号： A61K39/15 , C12N7/00 , C07K14/005
CPC分类号： A61K39/15 , A61K31/7088 , A61K39/12 , A61K2039/5252 , A61K2039/5254 , A61K2039/552 , A61K2039/70 , C07K14/005 , C07K16/10 , C12N7/00 , C12N2720/12022 , C12N2720/12034 , C12N2720/12222 , C12N2720/12234 , G01N33/5308 , Y02A50/403
摘要： The invention is directed to immunogenic compositions and methods for inducing an immune response against Piscine reoviruses in an animal. In another aspect, the invention relates to antibodies that bind Piscine reovirus polypeptides. In yet another aspect, the invention relates to methods for preventing, or reducing PRV infection in an animal.
摘要翻译：本发明涉及免疫原性组合物和用于诱导针对动物中的鱼类呼肠孤病毒的免疫应答的方法。另一方面，本发明涉及结合鱼类呼肠病毒多肽的抗体。在另一方面，本发明涉及用于预防或降低动物中PRV感染的方法。

3. 发明授权

US07915230B2 Reagents for transfection of eukaryotic cells 有权
标题翻译：用于转染真核细胞的试剂
公开(公告)号：US07915230B2
公开(公告)日：2011-03-29
申请号：US11434765
申请日：2006-05-17
申请人： Joel Jessee , Gulilat Gebeyehu
发明人： Joel Jessee , Gulilat Gebeyehu
IPC分类号： A61K48/00 , A61K38/16 , C12N15/00
CPC分类号： C07K14/005 , A61K47/6455 , C12N15/87 , C12N2720/12022
摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.
摘要翻译：提供了将大分子转运到真核细胞中的组合物和方法。来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。还提供了新的阳离子脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

4. 发明申请

US20050048075A1 Protein isolation 有权
标题翻译：蛋白质分离
公开(公告)号：US20050048075A1
公开(公告)日：2005-03-03
申请号：US10921741
申请日：2004-08-19
申请人： Yu-Chan Chao , Tzu-Ching Lee
发明人： Yu-Chan Chao , Tzu-Ching Lee
IPC分类号： A61K39/12 , C07H21/04 , C07K14/005 , C12N15/86 , C12P21/04 , C12Q1/70
CPC分类号： C12N7/00 , C07K2319/00 , C12N15/86 , C12N2710/14122 , C12N2710/14143 , C12N2720/12022 , C12N2770/32122 , C12N2810/40 , C12N2830/00 , C12N2830/60
摘要： A fusion protein containing a polyhedrin of a virus and a foreign polypeptide sequence. Also disclosed are a recombinant occlusion body containing the fusion protein, an isolated nucleic acid containing a sequence encoding the fusion protein, and a recombinant virus containing the nucleic acid. Within the scope of this invention are related expression vectors, host cells, compositions, and preparation methods.
摘要翻译：含有病毒多角体蛋白和外来多肽序列的融合蛋白。还公开了含有融合蛋白的重组闭塞体，含有编码融合蛋白的序列的分离的核酸和含有核酸的重组病毒。在本发明的范围内是相关的表达载体，宿主细胞，组合物和制备方法。

5. 发明授权

US4764460A Recombinant sigma NS protein 失效
标题翻译：重组sigma NS蛋白
公开(公告)号：US4764460A
公开(公告)日：1988-08-16
申请号：US796372
申请日：1985-11-08
申请人： Yasuhiro Furuichi , Michael A. Richardson
发明人： Yasuhiro Furuichi , Michael A. Richardson
IPC分类号： C12N15/09 , A61K35/74 , A61K35/76 , A61K38/00 , C07K14/14 , C07K17/02 , C07K17/14 , C12N1/00 , C12N5/10 , C12P21/00 , C12Q1/68 , C12Q1/70 , C12R1/07 , C12R1/125 , C12R1/19 , C12R1/38 , C12R1/645 , C12R1/91 , G01N33/50 , C12N15/00 , G01N33/566
CPC分类号： C07K14/005 , C12Q1/6804 , C12Q1/70 , C12Q1/701 , C12N2720/12022 , Y10S435/948
摘要： This disclosure relates to the synthesis by application of recombinant DNA technology of recombinant reovirus nonstructural protein sigma NS and its use as a non-specific binding agent for single stranded (ss) RNA's. This property of sigma NS protein can be employed when it is used as a reagent in protecting unstable RNA during extraction processes and more significantly as a reagent for the concentration of (ss) RNA samples for use in hybridization probe assays.
摘要翻译：本公开涉及通过应用重组呼肠弧病毒非结构蛋白质σNS的重组DNA技术的合成及其作为单链（ss）RNA的非特异性结合剂的用途。当sigma NS蛋白质在提取过程中用作保护不稳定RNA的试剂，并且更显着地作为用于杂交探针测定中使用的（ss）RNA样品的浓度的试剂时，可以使用该σ性质的NS蛋白质。

6. 发明授权

US08554493B2 Viral polyhedra complexes and methods of use 有权
标题翻译：病毒多面体复合物及其使用方法
公开(公告)号：US08554493B2
公开(公告)日：2013-10-08
申请号：US12529110
申请日：2008-02-28
申请人： Peter Metcalf , Fasseli Joseph Coulibaly , Hajime Mori , Norio Hamada , Keiko Ikeda , Yui Lam Elaine Chiu , Hiroshi Ijiri
发明人： Peter Metcalf , Fasseli Joseph Coulibaly , Hajime Mori , Norio Hamada , Keiko Ikeda , Yui Lam Elaine Chiu , Hiroshi Ijiri
IPC分类号： G06F19/00
CPC分类号： C07K14/005 , A01N25/34 , A01N63/00 , A61K47/6901 , A61K47/6949 , B82Y5/00 , C07K14/475 , C07K14/485 , C07K14/50 , C07K2319/735 , C12N2710/14043 , C12N2720/12022 , A01N61/00 , A01N63/02 , A01N2300/00
摘要： Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micron-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that like bacterial spores these insect viruses remain infectious for years in soil. Although these unique in vivo protein crystals have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using 5-12 micron crystals purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. It was found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into 3-D cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultra-stable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as in cell culture systems, microarrays and biopesticides.
摘要翻译：由于病毒颗粒嵌入称为多面体的微米级蛋白质晶体中，Cypoviruses和杆状病毒众所周知难以根除。多面体的显着稳定意味着像细菌孢子一样，这些昆虫病毒在土壤中仍然具有传染性多年。虽然这些独特的体内蛋白质晶体自20世纪初以来已被广泛地表征，但它们的原子组织仍然难以捉摸。这里我们描述使用从昆虫细胞纯化的5-12微米晶体测定的重组和感染性蚕病毒多面体的2晶体结构。这些是用于从头X射线蛋白质结构测定的最小晶体。发现多面体由病毒多角体蛋白的三聚体构成并含有核苷酸。尽管这些结构单元的形状让人联想到一些衣壳三聚体，但多角体蛋白具有新的折叠，并已发展成体内装配成3-D立方晶体而不是二十面体壳。多面体三聚体通过非共价相互作用在多面体中广泛交联，并具有类似于抗原 - 抗体复合物的精细分子互补性。所得到的超稳定和密封的晶体屏蔽病毒颗粒免受环境破坏。该结构表明，多面体可以作为开发用于生物技术应用的强壮和多功能纳米颗粒的基础，例如在细胞培养系统，微阵列和生物杀虫剂中。

7. 发明授权

US08323658B2 Infectious bursal disease virus antigenic isolates and vaccines 有权
标题翻译：传染性法氏囊病病毒抗原分离物和疫苗
公开(公告)号：US08323658B2
公开(公告)日：2012-12-04
申请号：US12914591
申请日：2010-10-28
申请人： Jeffrey Harold Rodenberg , Mahesh Kumar , Kalen Cookson
发明人： Jeffrey Harold Rodenberg , Mahesh Kumar , Kalen Cookson
IPC分类号： A61K39/12 , A61K39/295 , C07K14/08 , C12N7/00
CPC分类号： C07K14/005 , A61K39/12 , A61K39/15 , A61K2039/5252 , A61K2039/5254 , A61K2039/552 , A61K2039/55566 , A61K2039/70 , C12N7/00 , C12N2720/10022 , C12N2720/10034 , C12N2720/12022 , C12N2720/12034
摘要： Antigenic isolates and vaccines for Infectious Bursal Disease Virus include variants of the molecular Group 6 family of IBDV isolates, in particular the 28-1 isolate.
摘要翻译：用于传染性法氏囊病病毒的抗原分离物和疫苗包括IBDV分离株的分子6族家族的变体，特别是28-1分离株。

8. 发明授权

US07674606B2 Protein isolation 有权
标题翻译：蛋白质分离
公开(公告)号：US07674606B2
公开(公告)日：2010-03-09
申请号：US11873241
申请日：2007-10-16
申请人： Yu-Chan Chao , Tzu-Ching Lee
发明人： Yu-Chan Chao , Tzu-Ching Lee
IPC分类号： C12Q1/70 , C07H21/04 , C07K14/05
CPC分类号： C12N7/00 , C07K2319/00 , C12N15/86 , C12N2710/14122 , C12N2710/14143 , C12N2720/12022 , C12N2770/32122 , C12N2810/40 , C12N2830/00 , C12N2830/60
摘要： A fusion protein containing a polyhedrin of a virus and a foreign polypeptide sequence. Also disclosed are a recombinant occlusion body containing the fusion protein, an isolated nucleic acid containing a sequence encoding the fusion protein, and a recombinant virus containing the nucleic acid. Within the scope of this invention are related expression vectors, host cells, compositions, and preparation methods.
摘要翻译：含有病毒多角体蛋白和外来多肽序列的融合蛋白。还公开了含有融合蛋白的重组闭塞体，含有编码融合蛋白的序列的分离的核酸和含有核酸的重组病毒。在本发明的范围内是相关的表达载体，宿主细胞，组合物和制备方法。

9. 发明申请

US20080124793A1 MEMBRANE FUSION PROTEINS DERIVED FROM REOVIRUS 有权
标题翻译：从REOVIRUS衍生的膜蛋白融合蛋白
公开(公告)号：US20080124793A1
公开(公告)日：2008-05-29
申请号：US11952714
申请日：2007-12-07
申请人： Roy DUNCAN
发明人： Roy DUNCAN
IPC分类号： C12N5/06 , C07K14/00 , C07K16/18 , C12N15/11
CPC分类号： C07K14/005 , C12N2720/12022
摘要： In accordance with the present invention, a family of membrane fusion protein and polynucleotides encoding the proteins have been identified. The proteins and nucleotides are derived from the family Reoviridae. Two membrane fusion proteins have been isolated from reoviruses isolated from poikilothermic hosts: the p14 protein from reptilian reovirus (RRV) isolated from python, and the p16 protein from aquareovirus (AQV) isolated from salmon. The genes encoding these proteins have been cloned and sequenced. Analysis of the amino acid sequences of these proteins show that both lack the typical fusion peptide motif found in other membrane fusion proteins. Expression of these proteins in cells results in cell-cell fusion.
摘要翻译：根据本发明，已经鉴定了一系列膜融合蛋白和编码该蛋白的多核苷酸。蛋白质和核苷酸来源于家禽病毒科。从poikilothermic宿主分离的呼肠孤病毒中分离出两种膜融合蛋白：从蟒蛇分离的爬行动物呼肠孤病毒（RRV）的p14蛋白和从鲑鱼分离的来自水痘病毒（AQV）的p16蛋白。编码这些蛋白质的基因已被克隆并测序。这些蛋白质的氨基酸序列分析表明，缺乏其他膜融合蛋白中发现的典型融合肽基序。这些蛋白质在细胞中的表达导致细胞 - 细胞融合。

10. 发明申请

US20040059091A1 Protein complexes and process for producing the same 有权
标题翻译：蛋白复合物及其制备方法
公开(公告)号：US20040059091A1
公开(公告)日：2004-03-25
申请号：US10415096
申请日：2003-09-10
申请人： PROTEIN CRYSTAL CO., LTD.
发明人： Yoshimoto Ohta , Hajime Mori , Keiko Ikeda
IPC分类号： C07K014/005 , C12Q001/68 , C07H021/04 , C12N005/10 , C12N005/06 , C12N005/04
CPC分类号： C07K14/005 , C12N15/8257 , C12N15/86 , C12N2710/14143 , C12N2720/12022 , C12P21/02
摘要： A protein complex that is produced in cells and has a structure that the objective protein is occluded with the viral occlusion body protein. A protein complex having the structure that the objective protein is occluded with the viral occlusion body protein, produced in insect cells or plant cells, preferably produced by incorporation of the objective protein in the crystalline form during crystallization of polyhedron derived from polyhedrosis virus, preferably a protein complex in which the polyhedrin contributes to improvement of stability, or protection, or improvement of preservability, or combination thereof, of the objective protein.
摘要翻译：在细胞中产生并具有目标蛋白被病毒闭塞体蛋白吸留的结构的蛋白质复合物。具有在昆虫细胞或植物细胞中产生的目的蛋白被病毒闭塞体蛋白封闭的结构的蛋白质复合物，优选通过在衍生自多角体病毒的多面体结晶过程中结晶形成目标蛋白质而产生，优选为蛋白质复合物，其中多角体蛋白有助于提高目标蛋白质的稳定性或保护性，或其保存性或其组合的改善。

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