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1. 发明授权

US08554493B2 Viral polyhedra complexes and methods of use 有权
标题翻译：病毒多面体复合物及其使用方法
公开(公告)号：US08554493B2
公开(公告)日：2013-10-08
申请号：US12529110
申请日：2008-02-28
申请人： Peter Metcalf , Fasseli Joseph Coulibaly , Hajime Mori , Norio Hamada , Keiko Ikeda , Yui Lam Elaine Chiu , Hiroshi Ijiri
发明人： Peter Metcalf , Fasseli Joseph Coulibaly , Hajime Mori , Norio Hamada , Keiko Ikeda , Yui Lam Elaine Chiu , Hiroshi Ijiri
IPC分类号： G06F19/00
CPC分类号： C07K14/005 , A01N25/34 , A01N63/00 , A61K47/6901 , A61K47/6949 , B82Y5/00 , C07K14/475 , C07K14/485 , C07K14/50 , C07K2319/735 , C12N2710/14043 , C12N2720/12022 , A01N61/00 , A01N63/02 , A01N2300/00
摘要： Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micron-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that like bacterial spores these insect viruses remain infectious for years in soil. Although these unique in vivo protein crystals have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using 5-12 micron crystals purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. It was found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into 3-D cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultra-stable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as in cell culture systems, microarrays and biopesticides.
摘要翻译：由于病毒颗粒嵌入称为多面体的微米级蛋白质晶体中，Cypoviruses和杆状病毒众所周知难以根除。多面体的显着稳定意味着像细菌孢子一样，这些昆虫病毒在土壤中仍然具有传染性多年。虽然这些独特的体内蛋白质晶体自20世纪初以来已被广泛地表征，但它们的原子组织仍然难以捉摸。这里我们描述使用从昆虫细胞纯化的5-12微米晶体测定的重组和感染性蚕病毒多面体的2晶体结构。这些是用于从头X射线蛋白质结构测定的最小晶体。发现多面体由病毒多角体蛋白的三聚体构成并含有核苷酸。尽管这些结构单元的形状让人联想到一些衣壳三聚体，但多角体蛋白具有新的折叠，并已发展成体内装配成3-D立方晶体而不是二十面体壳。多面体三聚体通过非共价相互作用在多面体中广泛交联，并具有类似于抗原 - 抗体复合物的精细分子互补性。所得到的超稳定和密封的晶体屏蔽病毒颗粒免受环境破坏。该结构表明，多面体可以作为开发用于生物技术应用的强壮和多功能纳米颗粒的基础，例如在细胞培养系统，微阵列和生物杀虫剂中。

2. 外观设计

USD355451S Baseball pom-pom 失效
公开(公告)号：USD355451S
公开(公告)日：1995-02-14
申请号：US11592
申请日：1993-08-10
申请人： Peter Metcalf
设计人： Peter Metcalf

3. 外观设计

USD338930S Hockey pom-pom 失效
公开(公告)号：USD338930S
公开(公告)日：1993-08-31
申请号：US757520
申请日：1991-09-11
申请人： Laura Matchett , Peter Metcalf
设计人： Laura Matchett , Peter Metcalf

4. 发明申请

US20100216651A1 VIRAL POLYHEDRA COMPLEXES AND METHODS OF USE 有权
标题翻译： VIRAL POLYHEDRA复合物及其使用方法
公开(公告)号：US20100216651A1
公开(公告)日：2010-08-26
申请号：US12529110
申请日：2008-02-28
申请人： Peter Metcalf , Fasseli Joseph Coulibaly , Hajime Mori , Norio Hamada , Keiko Ikeda , Yui Lam Elaine Chiu , Hiroshi Ijiri
发明人： Peter Metcalf , Fasseli Joseph Coulibaly , Hajime Mori , Norio Hamada , Keiko Ikeda , Yui Lam Elaine Chiu , Hiroshi Ijiri
IPC分类号： C40B30/00 , C12N7/00 , C12N5/071
CPC分类号： C07K14/005 , A01N25/34 , A01N63/00 , A61K47/6901 , A61K47/6949 , B82Y5/00 , C07K14/475 , C07K14/485 , C07K14/50 , C07K2319/735 , C12N2710/14043 , C12N2720/12022 , A01N61/00 , A01N63/02 , A01N2300/00
摘要： Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micron-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that like bacterial spores these insect viruses remain infectious for years in soil. Although these unique in vivo protein crystals have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using 5-12 micron crystals purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. It was found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into 3-D cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultra-stable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as in cell culture systems, microarrays and biopesticides.
摘要翻译：由于病毒颗粒嵌入称为多面体的微米级蛋白质晶体中，Cypoviruses和杆状病毒众所周知难以根除。多面体的显着稳定意味着像细菌孢子一样，这些昆虫病毒在土壤中仍然具有传染性多年。虽然这些独特的体内蛋白质晶体自20世纪初以来已被广泛地表征，但它们的原子组织仍然难以捉摸。这里我们描述使用从昆虫细胞纯化的5-12微米晶体测定的重组和感染性蚕病毒多面体的2晶体结构。这些是用于从头X射线蛋白质结构测定的最小晶体。发现多面体由病毒多角体蛋白的三聚体构成并含有核苷酸。尽管这些结构单元的形状让人联想到一些衣壳三聚体，但多角体蛋白具有新的折叠，并已发展成体内装配成3-D立方晶体而不是二十面体壳。多面体三聚体通过非共价相互作用在多面体中广泛交联，并具有类似于抗原 - 抗体复合物的精细分子互补性。所得到的超稳定和密封的晶体屏蔽病毒颗粒免受环境破坏。该结构表明，多面体可以作为开发用于生物技术应用的强壮和多功能纳米颗粒的基础，例如在细胞培养系统，微阵列和生物杀虫剂中。

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