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1. 发明公开

US20240254461A1 Phytase Variants 审中-公开
公开(公告)号：US20240254461A1
公开(公告)日：2024-08-01
申请号：US18578921
申请日：2022-06-23
申请人： AB Enzymes Oy
发明人： Kari JUNTUNEN , Pihla AHOLA , Sven BENSON , Lenz LORENZ , Tara METZGER , Imke KÜHN , Terhi PURANEN , Marja PALOHEIMO
IPC分类号： C12N9/16 , A23K10/14 , A23K20/189 , A23K40/25 , A23K50/75
CPC分类号： C12N9/16 , A23K10/14 , A23K20/189 , A23K40/25 , A23K50/75 , C12Y301/03026
摘要： A phytase variant, a method for its manufacturing, an animal feed, a feed supplement, a dry and a liquid formulation, a method of degrading phytic acid, a method of manufacturing a phytase variant, a method of manufacturing a feed pellet, and a recombinant host cell configured to produce at least one polypeptide are disclosed, wherein the phytase variant comprises amino acid substitutions and has at least 95% amino acid sequence identity with amino acids of SEQ ID NO: 1.

2. 发明授权

US10570383B2 Variants of fungal cellulase 有权
公开(公告)号：US10570383B2
公开(公告)日：2020-02-25
申请号：US15850712
申请日：2017-12-21
申请人： AB Enzymes Oy
发明人： Kari Juntunen , Leena Valtakari , Susanna Mäkinen , Marika Alapuranen , Hendrik Hellmuth , Pentti Ojapalo , Marc Mennicken , Ulrich Schwaneberg , David Schönauer , Terhi Puranen
IPC分类号： C12N9/26 , C12N15/00 , C12N9/42 , C11D3/386 , C11D3/00 , C11D11/00 , D06M16/00
摘要： The present invention relates to polypeptides having cellulase activity, in particular to variants derived from the 20K-cellulase enzyme. The invention discloses a number of amino acid residue positions important for the properties of the cellulase enzyme and thereby for the stability and/or performance thereof. The novel variants have improved stability compared to the parental cellulase. In particular, the novel variants have good performance in an antigreying application and excellent stability in the presence of a protease in several detergent compositions even in long-term experiments.

3. 发明授权

US08945900B2 Variants of fungal serine protease 有权
标题翻译：真菌丝氨酸蛋白酶的变体
公开(公告)号：US08945900B2
公开(公告)日：2015-02-03
申请号：US13283891
申请日：2011-10-28
申请人： Kari Juntunen , Leena Valtakari , Nina Hakulinen , Marja Paloheimo
发明人： Kari Juntunen , Leena Valtakari , Nina Hakulinen , Marja Paloheimo
IPC分类号： C12N9/02 , C11D3/02 , C11D3/386 , C12N9/58
CPC分类号： C12N9/58 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants. The serine protease variants are applicable in laundry and dish-washing detergent compositions, in treating fibers, in treating wool, in treating hair, in treating leather, in treating food or feed, or in any application involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶变体，其包含母本镰刀菌属Fe_RF6318丝氨酸蛋白酶的208位的缬氨酸的氨基酸取代，其中取代的位置对应于SEQ ID NO：1中定义的成熟Fe_RF6318酶的氨基酸序列编号：2。与母体Fe_RF6318酶相比，变体具有改善的热稳定性和/或洗涤剂稳定性。优选地，取代是V208I，更优选地，变体包含进一步增加稳定性的另外的氨基酸变化。还公开了编码所述蛋白酶变体的核酸序列以及用于产生变体的重组载体和宿主细胞。丝氨酸蛋白酶变体可用于洗衣和洗碟洗涤剂组合物，处理纤维，处理羊毛，处理毛发，处理皮革，处理食品或饲料，或涉及任何涉及蛋白质物质的改性，降解或去除的应用。

4. 发明授权

US08937170B2 Nucleic acids encoding fungal protease 有权
标题翻译：编码真菌蛋白酶的核酸
公开(公告)号：US08937170B2
公开(公告)日：2015-01-20
申请号：US14066110
申请日：2013-10-29
申请人： AB Enzymes Oy
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
IPC分类号： C07H21/04 , C12N9/58 , C11D3/386
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌（Fusarium equiseti）获得，更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列，例如质粒pALK2521，其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO：9和质粒pALK2529，质粒pALK2529包含全长基因SEQ ID NO： 10，以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂，并用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质物质修饰，降解或去除的应用。

5. 发明授权

US08609390B2 Fungal serine protease and use thereof 有权
标题翻译：真菌丝氨酸蛋白酶及其用途
公开(公告)号：US08609390B2
公开(公告)日：2013-12-17
申请号：US12799638
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo , George Szakacs
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo , George Szakacs
IPC分类号： C12N9/68
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：18的氨基酸序列的成熟Fa_RF7182酶的氨基酸序列。丝氨酸蛋白酶可从尖锐镰孢获得，更优选来自保藏菌株CBS 还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22208沉积在大肠杆菌RF7803中的核苷酸序列SEQ ID NO：12的质粒pALK2530和保藏的全长基因SEQ ID NO：13的质粒pALK2531 在保藏号DSM 22209的大肠杆菌RF7879中，以及真菌宿主如Trichoderm。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。

6. 发明申请

US20120252064A1 Protease Enzyme and Uses Thereof 有权
标题翻译：蛋白酶及其用途
公开(公告)号：US20120252064A1
公开(公告)日：2012-10-04
申请号：US13433984
申请日：2012-03-29
申请人： Leena Valtakari , Kari Juntunen , Marja Paloheimo , Pentti Ojapalo
发明人： Leena Valtakari , Kari Juntunen , Marja Paloheimo , Pentti Ojapalo
IPC分类号： C12N9/58 , C12N1/15 , D06M16/00 , C12S11/00 , A23J3/34 , C11D3/386 , C11D17/00 , C12P21/06 , C12N15/57 , C12N15/80 , C12N9/96
CPC分类号： C11D3/386 , C11D3/2065 , C11D11/0017 , C11D11/0023 , C12N9/58 , C12Y304/21
摘要： The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of Malbranchea ALKO4122 mature protease as defined in SEQ ID NO:18 or an amino acid sequence having at least 66% identity to the amino acid sequence of SEQ ID NO:18. Also disclosed is an isolated nucleic acid molecule, comprising a polynucleotide sequence which encodes a fungal serine protease enzyme, nucleic acid sequences encoding said protease, a host cell and a process of producing a polypeptide having serine protease activity. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, wool, hair, leather, or silk, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其中所述酶具有丝氨酸蛋白酶活性，并且包含如SEQ ID NO：18所定义的Malbranchea ALKO4122成熟蛋白酶的氨基酸序列或与SEQ ID NO：18所定义的氨基酸序列具有至少66％同一性的氨基酸序列 SEQ ID NO：18的氨基酸序列。还公开了分离的核酸分子，其包含编码真丝丝氨酸蛋白酶的多核苷酸序列，编码所述蛋白酶的核酸序列，宿主细胞和产生具有丝氨酸蛋白酶活性的多肽的方法。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，羊毛，毛发，皮革或丝绸，用于处理食品或饲料，或用于涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。

7. 发明申请

US20110269213A1 Fungal Endoglucanases, Their Production and Use 有权
标题翻译：真菌内切葡聚糖酶，其生产和使用
公开(公告)号：US20110269213A1
公开(公告)日：2011-11-03
申请号：US13142630
申请日：2009-12-28
申请人： Terhi Puranen , Leena Valtakari , Marika Alapuranen , George Szakacs , Pentti Ojapalo , Jari Vehmaanperä
发明人： Terhi Puranen , Leena Valtakari , Marika Alapuranen , George Szakacs , Pentti Ojapalo , Jari Vehmaanperä
IPC分类号： C12N9/42 , C12N15/63 , C12N1/15 , C12N1/19 , A23K1/00 , C12N1/20 , C12N5/10 , C12S99/00 , C12S11/00 , A23L1/00 , C07H21/04 , C12N1/21
CPC分类号： C12N9/2437 , A23K20/189 , C12P19/02 , C12P19/14 , C12Y302/01004 , D06M16/00 , D21C5/005 , Y02P20/52
摘要： Novel fungal endoglucanases Cel5 and Cel2 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production.
摘要翻译：公开了新型真菌内切葡聚糖酶Cel5和Cel2。内切葡聚糖酶通过重组技术方便地制备，并描述了其生产手段。内切葡聚糖酶用于处理纤维素材料，特别是在纺织工业中。在生物整理或生物印记。它们也可用于洗涤剂，动物饲料和/或纸浆和造纸工业中，或用于木质纤维素材料的水解。生物乙醇生产。

8. 发明授权

US07741093B2 Cellulases and their uses 有权
标题翻译：纤维素酶及其用途
公开(公告)号：US07741093B2
公开(公告)日：2010-06-22
申请号：US11119526
申请日：2005-04-29
申请人： Jari Vehmaanperä , Terhi Puranen , Leena Valtakari , Jarno Kallio , Marika Alapuranen , Marja Paloheimo , Pentti Ojapalo
发明人： Jari Vehmaanperä , Terhi Puranen , Leena Valtakari , Jarno Kallio , Marika Alapuranen , Marja Paloheimo , Pentti Ojapalo
IPC分类号： C12N9/42 , C12N9/00 , C02F3/34 , D06M16/00 , C11D3/386 , C11D3/00 , C07H21/04
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： The present invention provides novel cellulase fusion proteins, preparations of cellulase fusion proteins and compositions of cellulase fusion proteins. The present invention further provides cellulase expression vectors, host cells expressing cellulase and methods for preparing such vectors and cells. Uses of cellulases, cellulase preparations and cellulase compositions in the textile, detergent, pulp and paper industries are also provided.
摘要翻译：本发明提供新型纤维素酶融合蛋白，纤维素酶融合蛋白的制备物和纤维素酶融合蛋白的组合物。本发明还提供了纤维素酶表达载体，表达纤维素酶的宿主细胞和用于制备这种载体和细胞的方法。还提供了纤维素酶，纤维素酶制剂和纤维素酶组合物在纺织品，洗涤剂，纸浆和造纸工业中的使用。

9. 发明申请

US20080248016A1 Novel Iaccase Enzymes and Their Uses 有权
标题翻译：新型致病酶及其用途
公开(公告)号：US20080248016A1
公开(公告)日：2008-10-09
申请号：US11663424
申请日：2005-09-21
申请人： Marja Paloheimo , Terhi Puranen , Leena Valtakari , Kristiina Kruus , Jarno Kallio , Arja Mantyla , Richard Fagerstrom , Pentti Ojapalo , Jari Vehmaanpera
发明人： Marja Paloheimo , Terhi Puranen , Leena Valtakari , Kristiina Kruus , Jarno Kallio , Arja Mantyla , Richard Fagerstrom , Pentti Ojapalo , Jari Vehmaanpera
IPC分类号： A61K38/43 , C12N9/02 , C12N15/11 , C12N15/00 , A61P43/00 , C12N1/00 , C12P21/04 , D06M16/00
CPC分类号： D06M16/003 , A61K8/66 , A61Q5/00 , A61Q5/08 , C11D3/38654 , C12N9/0061 , D06L4/40 , D06P5/137 , D06P5/158 , D21C5/005 , D21H17/005
摘要： The present invention relates to novel laccase enzymes obtainable from the strains of the genus Thielavia or from the strains of the genus Chaetomium. The invention relates also to nucleic acid sequences encoding the enzymes, recombinant hosts into which the nucleic acid sequences have been introduced and to methods for the production of the enzymes in recombinant hosts. The enzymes of the invention are suitable for several applications, for example for treating denim and for strain removal.
摘要翻译：本发明涉及可从Thielavia属的菌株或Chaetomium属的菌株获得的新的漆酶。本发明还涉及编码酶的核酸序列，其中引入了核酸序列的重组宿主和在重组宿主中产生酶的方法。本发明的酶适用于几种用途，例如用于处理牛仔布和除去菌株。

10. 发明申请

US20210395719A1 Method of Precipitating Phytase 有权
公开(公告)号：US20210395719A1
公开(公告)日：2021-12-23
申请号：US17288676
申请日：2019-11-06
申请人： AB Enzymes Oy
发明人： Katja PALMUNEN , Mirkka PERKKALAINEN , Leena LEHTIKARI , Imke KÜHN
IPC分类号： C12N9/96 , C12N9/16
摘要： Herein is disclosed a process for effectively precipitating phytase as a complex with a polyanion, as well as compositions comprising phytase and a polyanion, and a method for manufacturing such compositions.

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