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1. 发明授权

US06899800B2 Polymeric electrode for electrophysiological testing 失效
标题翻译：用于电生理测试的聚合物电极
公开(公告)号：US06899800B2
公开(公告)日：2005-05-31
申请号：US10176183
申请日：2002-06-20
申请人： Yuri Osipchuk , Alexei Savtchenko , Alexander A. Dromaretsky
发明人： Yuri Osipchuk , Alexei Savtchenko , Alexander A. Dromaretsky
IPC分类号： B01L3/00 , G01N33/487 , G01N27/327 , C12M1/34 , C12N13/00
CPC分类号： G01N33/48728 , B01L3/5085
摘要： A polymeric material such as PDMS is molded into an electrode structure containing a micron-size aperture for receiving and forming a giga-ohm seal with a biological membrane. One end of a tube is filled with uncured polymeric material and pressed against a support surface to prevent drainage. A conventional micropipette having a size suitable for sliding through the tube is introduced, tip first, into the tube and is allowed to fall through the polymeric material and rest against the support surface. The assembly is heated to cure the polymer and the micropipette is removed from the tube, thereby leaving a polymeric plug at the end of the tube with an aperture suitable in shape and size for patch-clamp giga-ohm seal electrode applications. A multi-well tray with a polymeric electrode plug in each well is constructed using the same approach.
摘要翻译：诸如PDMS的聚合物材料被模制成包含微米级孔径的电极结构，用于接收并用生物膜形成千兆欧密封。管的一端填充有未固化的聚合物材料并压靠支撑表面以防止排水。将具有适合于穿过管滑动的尺寸的常规微量移液管首先引入管中并且允许其通过聚合物材料落下并靠在支撑表面上。将组件加热以固化聚合物，并从管中移除微量移液管，从而在管的端部留下聚合物塞，其孔适于用于膜片钳千兆欧密封电极应用的形状和尺寸。使用相同的方法构建在每个孔中具有聚合物电极塞的多孔盘。

2. 发明授权

US07056430B1 Detachable cell-delivery system for patch-clamp unit 有权
标题翻译：用于膜片钳单元的可拆卸细胞递送系统
公开(公告)号：US07056430B1
公开(公告)日：2006-06-06
申请号：US10339142
申请日：2003-01-09
申请人： Yuri Osipchuk , Yuriy Roll , Alan S. Finkel , Alexander A. Dromaretsky , Branko Bem
发明人： Yuri Osipchuk , Yuriy Roll , Alan S. Finkel , Alexander A. Dromaretsky , Branko Bem
IPC分类号： G01N33/487
CPC分类号： G01N33/48728
摘要： The cell-delivery unit of a high-throughput electrophysiological testing system is implemented as a reusable movable unit suitable for repetitive delivery of cells to a disposable, multi-aperture patch-clamp tray. An electric field emanating from the patch aperture is used to align the dispenser with the aperture. A set of electrodes in the nozzle of the dispenser is used to detect the electric field and effect the alignment. According to another aspect of the invention, dielectrophoretic fields produced by sets of electrodes in the nozzle form a retaining cage that is used first to suspend a test cell directly above the patch aperture and then to urge the cell toward it. A movable cell sorter may also be coupled to the cell-delivery unit.
摘要翻译：高通量电生理测试系统的细胞传递单元被实施为可重复使用的可移动单元，其适用于将细胞重复地递送到一次性多孔膜片钳夹盘。使用从贴片孔发出的电场使分配器与孔对准。使用分配器喷嘴中的一组电极来检测电场并进行对准。根据本发明的另一方面，通过喷嘴中的电极组产生的介电电泳场形成保持架，其首先用于将测试电池直接悬挂在贴片孔的上方，然后将电池推向电池。移动细胞分选器也可以耦合到细胞递送单元。

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