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1. 发明授权

US4618485A Kinetic radioimmunoassay test method and device 失效
标题翻译：动力放射免疫测定方法及装置
公开(公告)号：US4618485A
公开(公告)日：1986-10-21
申请号：US357399
申请日：1982-03-12
申请人： Yuh-Geng Tsay , Vipin D. Shah
发明人： Yuh-Geng Tsay , Vipin D. Shah
IPC分类号： G01N33/543 , G01N33/557 , G01N33/56 , G01N33/78
CPC分类号： G01N33/54306 , G01N33/557 , Y10S436/804
摘要： An improved radioimmunoassay test method and device based upon competitive binding test methods wherein immunoreactions are halted at a time when the rate of change of the quantity of bound radiolabeled analyte of interest is inversely proportional to the concentration of analyte of interest in an unknown sera. Based thereon, a test device is created having a single calibration curve 36 which is accurate throughout the shelf life of the device.
摘要翻译：一种改进的放射免疫测定方法和装置，其基于竞争性结合试验方法，其中当目标结合的放射性标记的分析物的量的变化速率与未知血清中感兴趣的分析物的浓度成反比时，其中免疫反应停止。基于此，产生具有单个校准曲线36的测试装置，其在装置的整个保质期内是准确的。

2. 发明授权

US5128240A Immunological method of measuring unstable analytes using cross-reactive antibodies 失效
标题翻译：使用交叉反应抗体测量不稳定分析物的免疫学方法
公开(公告)号：US5128240A
公开(公告)日：1992-07-07
申请号：US296542
申请日：1989-01-12
申请人： Vipin D. Shah
发明人： Vipin D. Shah
IPC分类号： G01N33/53 , G01N33/537 , G01N33/543 , G01N33/94
CPC分类号： G01N33/537 , G01N33/53 , G01N33/54306 , G01N33/9446 , Y10S435/961 , Y10S436/815 , Y10S436/822 , Y10S436/824 , Y10S436/825
摘要： A quantitative immunoassay for a beta-lactam antibiotic in a sample, which may contain open and closed ring forms of the antibiotic, is disclosed. Closed-ring forms of the antibiotic in the sample are converted to open-ring protein conjugates and detected through immunospecific reaction with antibodies raised against the open-ring protein conjugate form of the beta-lactam antibiotic.
摘要翻译：公开了一种样品中β-内酰胺抗生素的定量免疫测定，其可以含有开环和封闭环形式的抗生素。将样品中的抗生素的闭环形式转化为开环蛋白缀合物，并通过与针对β-内酰胺抗生素的开环蛋白缀合物形式产生的抗体的免疫特异性反应进行检测。

3. 发明授权

US5382522A Immunoassay for creatine kinase-MB and creatine kinase-BB isoforms and reagents 失效
标题翻译：肌酸激酶-MB和肌酸激酶-BB异构体和试剂的免疫测定
公开(公告)号：US5382522A
公开(公告)日：1995-01-17
申请号：US83854
申请日：1993-06-25
申请人： Vipin D. Shah , Shing-Erh Yen , Gerald M. Anchin
发明人： Vipin D. Shah , Shing-Erh Yen , Gerald M. Anchin
IPC分类号： G01N33/53 , G01N33/573 , C07K15/28
CPC分类号： G01N33/53 , G01N33/573 , Y10S436/811 , Y10S436/815
摘要： Methods and reagents for determining the lapse of time since an acute disease event, such as the occurrence of a myocardial infarction, are presented. A serum or plasma sample is assayed to determine the concentration of two different analytes selected from the group consisting of creatine kinase-MB species and creatine kinase-BB species. From these measurements, the time of the acute event can be more accurately determined. Novel antibodies, labeled and insolubilized derivatives of these antibodies, labeled proteins, and kits containing one or more of these reagents are also described.
摘要翻译：提出了用于确定急性疾病事件之后的时间流逝的方法和试剂，例如心肌梗死的发生。测定血清或血浆样品以确定选自肌酸激酶-MB和肌酸激酶-BB物种的两种不同分析物的浓度。从这些测量可以更精确地确定急性事件的时间。还描述了新型抗体，这些抗体的标记和不溶性衍生物，标记的蛋白质和含有一种或多种这些试剂的试剂盒。

4. 发明授权

US5382515A Creative kinase-MB immunoassay for myocardial infarction and reagents 失效
标题翻译：用于心肌梗塞和试剂的创新激酶-MB免疫测定
公开(公告)号：US5382515A
公开(公告)日：1995-01-17
申请号：US83853
申请日：1993-06-25
申请人： Vipin D. Shah , Shing-Erh Yen , Gerald M. Anchin
发明人： Vipin D. Shah , Shing-Erh Yen , Gerald M. Anchin
IPC分类号： C07K14/47 , C07K16/18 , C07K16/40 , C12N9/04 , G01N33/573 , G01N33/53 , C07K15/28
CPC分类号： C12N9/0006 , C07K14/47 , C07K16/18 , C07K16/40 , G01N33/573 , Y10S436/811 , Y10S436/815
摘要： Methods and reagents for determining the lapse of time since an acute disease event, such as the occurrence of a myocardial infarction, are presented. A serum or plasma sample is assayed to determine the concentration of two different analytes selected from a group of creatine kinase-MB species. From these measurements, the time of the acute event can be more accurately determined. Novel antibodies, labeled and insolubilized derivatives of these antibodies, labeled proteins, and kits containing one or more of these reagents are also described.
摘要翻译：提出了用于确定急性疾病事件之后的时间流逝的方法和试剂，例如心肌梗死的发生。测定血清或血浆样品以确定选自一组肌酸激酶-MB物种的两种不同分析物的浓度。从这些测量可以更精确地确定急性事件的时间。还描述了新型抗体，这些抗体的标记和不溶性衍生物，标记的蛋白质和含有一种或多种这些试剂的试剂盒。

5. 发明授权

US5198365A Fecal sample immunoassay method testing for hemoglobin 失效
标题翻译：粪便样本免疫测定法测定血红蛋白
公开(公告)号：US5198365A
公开(公告)日：1993-03-30
申请号：US764012
申请日：1991-09-23
申请人： Michael A. Grow , Vipin D. Shah
发明人： Michael A. Grow , Vipin D. Shah
IPC分类号： A61B10/00 , A61B19/02 , G01N33/52 , G01N33/72
CPC分类号： G01N33/725 , G01N33/528 , G01N33/721 , G01N33/726 , A61B10/0038 , A61B2050/0064 , Y10S436/815 , Y10S436/825 , Y10T436/10 , Y10T436/107497 , Y10T436/25375
摘要： A method for preparing a fecal sample for immunoassay testing comprising the steps of dispersing a sample of from 1 to less than 10 wt. % of a stool sample in an aqueous fecal test solution formulated with one or more preservatives, analyte stabilizing agents and endogenous interference reducing agents. The fecal solids are then permitted to settle to form a liquid phase substantially free from fecal solids, and the clarified liquid phase is removed to provide a test sample free from fecal solids. The fecal test solutions contain suitable stool stabilizers such as buffers and antimicrobial agents, analyte protecting agents such as proteolytic, reductive or oxidative enzyme inhibitors, endogenous assay interfering enzyme inhibitors such as a reducing agent, and non-specific binding inhibitors such as animal proteins. The stool sample should be fresh or be fresh frozen and thawed immediately before dispersion in the buffer solution. The sample is suitable for any solid-phase immunoassay determination of a fecal sample analyte. A method for determining analyte in the stool sample comprises conjugating anti-analyte antibody adhered to a insoluble support with analyte in the clarified sample, and determining the presence and extent of such conjugation. For determining human hemoglobin in a sample of human stool, the aqueous fecal test solution preferably contains a proteinase inhibitor, formaldehyde and an animal albumin.
摘要翻译：一种制备用于免疫测定试验的粪便样品的方法，包括以下步骤：将1至小于10wt。用一种或多种防腐剂，分析物稳定剂和内源性干扰还原剂配制的含水粪便试验溶液中的粪便样品的百分比。然后允许粪便固体沉降以形成基本上不含粪便固体的液相，并除去澄清的液相以提供不含粪便固体的测试样品。粪便测试溶液含有合适的粪便稳定剂如缓冲液和抗微生物剂，分析物保护剂如蛋白水解酶，还原酶或氧化酶抑制剂，内源性测定干扰酶抑制剂如还原剂和非特异性结合抑制剂如动物蛋白质。粪便样品应该是新鲜的，或者在分散在缓冲溶液中之前立即新鲜冷冻和解冻。样品适用于粪便样品分析物的任何固相免疫测定。用于测定粪便样品中的分析物的方法包括将附着于不溶性载体的抗分析物抗体与澄清样品中的分析物缀合，并确定这种缀合的存在和程度。为了测定人粪便样品中的人血红蛋白，粪便测试溶液优选含有蛋白酶抑制剂，甲醛和动物白蛋白。

6. 发明授权

US4624916A Process and composition for the rapid quantitation of small levels of creative kinase-MB isoenzyme 失效
标题翻译：用于快速定量小水平创造性激酶-MB同工酶的方法和组成
公开(公告)号：US4624916A
公开(公告)日：1986-11-25
申请号：US597593
申请日：1984-04-06
申请人： Vipin D. Shah , Nila V. Shah
发明人： Vipin D. Shah , Nila V. Shah
IPC分类号： G01N33/543 , G01N33/573 , A61K39/00 , G01N33/53
CPC分类号： G01N33/573 , G01N33/54306 , Y10S435/81 , Y10S435/962 , Y10S435/975 , Y10S436/808 , Y10S436/811 , Y10S436/825 , Y10S436/826
摘要： A process for conducting fast, rapid two site, two step immunoradiometric tests for CK-MB.In a first series of steps, a mobile particulate solid-phase having an isoenzyme selected from the group of CK-BB and CK-MM immobilying bound on its surface is suspended in a matrix of pooled match human serum. The mixture is incubated, diluted, and centrifuged.In a second series of steps, the resulting solid is incubated with a radio labeled antibody that is specific to the isoenzyme not used in the first series of steps. Counting the radioactivity gives the amount of CK-MB in the patients serum.
摘要翻译：为CK-MB进行快速，快速两个站点，两步免疫放射测试的过程。在第一系列步骤中，具有选自固定在其表面上的CK-BB和CK-MM组的同工酶的移动颗粒状固相悬浮在合并的人血清的基质中。将混合物温育，稀释并离心。在第二系列步骤中，将所得固体与第一系列步骤中未使用的同工酶特异的放射性标记抗体一起温育。计数放射性给出患者血清中的CK-MB量。

7. 发明授权

US5094956A Fecal sample immunoassay composition and method 失效
标题翻译： FECAL样品免疫组合物和方法
公开(公告)号：US5094956A
公开(公告)日：1992-03-10
申请号：US329455
申请日：1989-03-28
申请人： Michael A. Grow , Vipin D. Shah
发明人： Michael A. Grow , Vipin D. Shah
IPC分类号： G01N33/48 , A61B10/00 , A61B19/02 , G01N33/52 , G01N33/53 , G01N33/72
CPC分类号： G01N33/725 , G01N33/528 , G01N33/721 , G01N33/726 , A61B10/0038 , A61B2050/0064 , Y10S436/815 , Y10S436/825 , Y10T436/10 , Y10T436/107497 , Y10T436/25375
摘要： A method for preparing a fecal sample of immunoassay testing comprising the steps of dispersing a sample of from 1 to less than 10 wt. % of a stool sample in an aqueous fecal test solution formulated with one or more preservatives, analyte stabilizing agents and endogenous interference reducing agents. The fecal solids are then permitted to settle to form a liquid phase substantially free from fecal solids, and the clarified liquid phase is removed to provide a test sample free from fecal solids. The fecal test solutions contain suitable stool stabilizers such as buffers and antimicrobial agents, analyte protecting agents such as proteolytic, reductive or oxidative enzyme inhibitors, endogenous assay interfering enzyme inhibitors such as a reducing agent, and non-specific binding inhibitors such as animal proteins. The stool sample should be fresh or be fresh frozen and thawed immediately before dispersion in the buffer solution. The sample is suitable for any solid-phase immunoassay determination of a fecal sample analyte. A method for determining analyte in a stool sample comprises conjugating anti-analyte antibody adhered to a insoluble support with analyte in the clarified sample, and determining the presence and extent of such conjugation. For determining human hemoglobin in a sample of human stool, the aqueous fecal test solution preferably contains a proteinase inhibitor, formaldehyde and an animal albumin.

8. 发明授权

US5009996A Two site cross-reaction immunometric sandwich assay method 失效
标题翻译：两个位点交叉反应免疫夹心法
公开(公告)号：US5009996A
公开(公告)日：1991-04-23
申请号：US537947
申请日：1983-09-30
申请人： Vipin D. Shah , Padmaker V. Kulkarni , Quirinus R. Miranda
发明人： Vipin D. Shah , Padmaker V. Kulkarni , Quirinus R. Miranda
IPC分类号： G01N33/544 , G01N33/573
CPC分类号： G01N33/544 , G01N33/573 , Y10S435/805 , Y10S435/81
摘要： A two site cross-reaction immunometric sandwich assay method for the detection and measurement of an analyte, such as creatine phospho-kinase-MB, in serum comprising the selection of two different antibodies each of which is specific to a different analyte but each of which will cross-react with the analyte of interest. The first antibody is reacted with the unknown sample utilizing a solid-phase to bind the first antibody. Separation of the solid and liquid portions of the first reaction is accomplished and the solid portion thereof is reacted with the second antibody which is tagged. The solid portion and liquid portion of the second reaction are separated and the solid portion is tested for the tag as an indication of the presence of said analyte. With particular reference to testiong for creatine phospho-kinase-MB in human serum, the cross-reacting antibodies utilized are antibody to creatine phospho-kinase-BB and creatine phospho-kinase-MM.
摘要翻译：用于在血清中检测和测量分析物如肌酸磷酸激酶-MB的两个位点交叉反应免疫三明治测定方法，包括选择两种不同的抗体，每种抗体对不同的分析物是特异性的，但是每种将与感兴趣的分析物交叉反应。使用固相将第一抗体与未知样品反应以结合第一抗体。完成第一反应的固体和液体部分的分离，其固体部分与标记的第二抗体反应。分离第二反应的固体部分和液体部分，并测试固体部分作为所述分析物的存在的指示。特别参考人血清中肌酸磷酸激酶-MB的检测，所用的交叉反应抗体是肌酸磷酸激酶-BB和肌酸磷酸激酶-MN的抗体。

9. 发明授权

US5202234A Myocardial infarction immunoassay 失效
标题翻译：心肌梗塞免疫测定
公开(公告)号：US5202234A
公开(公告)日：1993-04-13
申请号：US299277
申请日：1989-01-23
申请人： Vipin D. Shah , Shing-Erh Yen , Gerald M. Anchin
发明人： Vipin D. Shah , Shing-Erh Yen , Gerald M. Anchin
IPC分类号： C12Q1/00 , C07K14/47 , C07K16/18 , C07K16/40 , C12N9/04 , C12Q1/50 , G01N33/573
CPC分类号： C12N9/0006 , C07K14/47 , C07K16/18 , C07K16/40 , G01N33/573 , Y10S436/811 , Y10S436/815
摘要： Methods and reagents for determining the lapse of time since an acute disease event, such as the occurrence of a myocardial infarction, are presented. A serum sample is assayed to determine the concentration of two analyte sets. From these measurements, the time of the acute event can be more accurately determined. Novel antibodies, labeled and insolubilized derivatives of these antibodies, labeled proteins, and kits containing one or more of these reagents are also described.
摘要翻译：提出了用于确定急性疾病事件之后的时间流逝的方法和试剂，例如心肌梗死的发生。测定血清样品以确定两个分析物组的浓度。从这些测量可以更精确地确定急性事件的时间。还描述了新型抗体，这些抗体的标记和不溶性衍生物，标记的蛋白质和含有一种或多种这些试剂的试剂盒。

10. 发明授权

US5009997A Two site cross-reaction immunometric sandwich assay method 失效
标题翻译：两个位点交叉反应免疫夹心法
公开(公告)号：US5009997A
公开(公告)日：1991-04-23
申请号：US896353
申请日：1985-01-30
申请人： Vipin D. Shah , Padmakar v. Kulkarni , Quirinus R. Miranda
发明人： Vipin D. Shah , Padmakar v. Kulkarni , Quirinus R. Miranda
IPC分类号： G01N33/544 , G01N33/573
CPC分类号： G01N33/573 , G01N33/544 , Y10S435/805 , Y10S435/81 , Y10S435/975 , Y10S436/808
摘要： A two site cross-reaction immunometric sandwich assay method for the detection and measurement of an analyte, such as creatine phospho-kinase-MB, in serum comprising the selection of two different antibodies each of which is specific to a different analyte but each of which will cross-react with the analyte of interest. The first antibody is reacted with the unknown sample utilizing a solid-phase to bind the first antibody. Separation of the solid and liquid portions of the first reaction is accomplished and the solid portion thereof is reacted with the second antibody which is tagged. The solid portion and liquid portion of the second reaction are separated and the solid portion is tested for the tag as an indication of the presence of said analyte. With particular reference to testing for creatine phospho-kinase-MB in human serum, the cross-reacting antibodies utilized are antibody to creatine phospho-kinase-BB and creatine phospho-kinase-MM.
摘要翻译：用于在血清中检测和测量分析物如肌酸磷酸激酶-MB的两个位点交叉反应免疫三明治测定方法，包括选择两种不同的抗体，每种抗体对不同的分析物是特异性的，但是每种将与感兴趣的分析物交叉反应。使用固相将第一抗体与未知样品反应以结合第一抗体。完成第一反应的固体和液体部分的分离，其固体部分与标记的第二抗体反应。分离第二反应的固体部分和液体部分，并测试固体部分作为所述分析物的存在的指示。特别参考人血清中肌酸磷酸激酶-MB的检测，所用的交叉反应抗体是针对肌酸磷酸激酶-BB和肌酸磷酸激酶-MM的抗体。

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