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2. 发明授权

US5198365A Fecal sample immunoassay method testing for hemoglobin 失效
标题翻译：粪便样本免疫测定法测定血红蛋白
公开(公告)号：US5198365A
公开(公告)日：1993-03-30
申请号：US764012
申请日：1991-09-23
申请人： Michael A. Grow , Vipin D. Shah
发明人： Michael A. Grow , Vipin D. Shah
IPC分类号： A61B10/00 , A61B19/02 , G01N33/52 , G01N33/72
CPC分类号： G01N33/725 , G01N33/528 , G01N33/721 , G01N33/726 , A61B10/0038 , A61B2050/0064 , Y10S436/815 , Y10S436/825 , Y10T436/10 , Y10T436/107497 , Y10T436/25375
摘要： A method for preparing a fecal sample for immunoassay testing comprising the steps of dispersing a sample of from 1 to less than 10 wt. % of a stool sample in an aqueous fecal test solution formulated with one or more preservatives, analyte stabilizing agents and endogenous interference reducing agents. The fecal solids are then permitted to settle to form a liquid phase substantially free from fecal solids, and the clarified liquid phase is removed to provide a test sample free from fecal solids. The fecal test solutions contain suitable stool stabilizers such as buffers and antimicrobial agents, analyte protecting agents such as proteolytic, reductive or oxidative enzyme inhibitors, endogenous assay interfering enzyme inhibitors such as a reducing agent, and non-specific binding inhibitors such as animal proteins. The stool sample should be fresh or be fresh frozen and thawed immediately before dispersion in the buffer solution. The sample is suitable for any solid-phase immunoassay determination of a fecal sample analyte. A method for determining analyte in the stool sample comprises conjugating anti-analyte antibody adhered to a insoluble support with analyte in the clarified sample, and determining the presence and extent of such conjugation. For determining human hemoglobin in a sample of human stool, the aqueous fecal test solution preferably contains a proteinase inhibitor, formaldehyde and an animal albumin.
摘要翻译：一种制备用于免疫测定试验的粪便样品的方法，包括以下步骤：将1至小于10wt。用一种或多种防腐剂，分析物稳定剂和内源性干扰还原剂配制的含水粪便试验溶液中的粪便样品的百分比。然后允许粪便固体沉降以形成基本上不含粪便固体的液相，并除去澄清的液相以提供不含粪便固体的测试样品。粪便测试溶液含有合适的粪便稳定剂如缓冲液和抗微生物剂，分析物保护剂如蛋白水解酶，还原酶或氧化酶抑制剂，内源性测定干扰酶抑制剂如还原剂和非特异性结合抑制剂如动物蛋白质。粪便样品应该是新鲜的，或者在分散在缓冲溶液中之前立即新鲜冷冻和解冻。样品适用于粪便样品分析物的任何固相免疫测定。用于测定粪便样品中的分析物的方法包括将附着于不溶性载体的抗分析物抗体与澄清样品中的分析物缀合，并确定这种缀合的存在和程度。为了测定人粪便样品中的人血红蛋白，粪便测试溶液优选含有蛋白酶抑制剂，甲醛和动物白蛋白。

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