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    • 5. 发明授权
    • Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
    • 棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
    • US07297521B2
    • 2007-11-20
    • US11011701
    • 2004-12-15
    • Yoko KuwabaraKenichi HashiguchiTsuyoshi NakamatsuOsamu KurahashiYukiko MoriHisao Ito
    • Yoko KuwabaraKenichi HashiguchiTsuyoshi NakamatsuOsamu KurahashiYukiko MoriHisao Ito
    • C12P13/10
    • C12N9/93C12P13/10
    • A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
    • 编码以下(a)或(b)中定义的多肽的DNA片段和以下(c)或(d)中定义的多肽:(a)至少具有氨基酸的氨基酸序列的多肽 序列表所示的SEQ ID NO:2中的50〜393的酸值,(b)至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽,包括取代, 一个或几个氨基酸的缺失,插入,添加或倒位,并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质,其具有氨基酸序列SEQ ID NO:3的氨基甲酰磷酸合成酶的大亚基,(c )具有序列表所示的SEQ ID NO:3的氨基酸序列的多肽,(d)具有序列表所示的SEQ ID NO:3的氨基酸序列的多肽,包括取代,缺失,插入,添加, 或反转一个或几个氨基酸,并可构成 具有氨基酸 - 磷酸合成酶活性的蛋白质,其具有SEQ ID NO:2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。
    • 6. 发明授权
    • Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
    • 棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
    • US06881566B2
    • 2005-04-19
    • US09836470
    • 2001-04-18
    • Yoko KuwabaraKenichi HashiguchiTsuyoshi NakamatsuOsamu KurahashiYukiko MoriHisao Ito
    • Yoko KuwabaraKenichi HashiguchiTsuyoshi NakamatsuOsamu KurahashiYukiko MoriHisao Ito
    • C12N9/00C12P13/10C12N1/20C07H21/04
    • C12P13/10C12N9/93
    • A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
    • 编码以下(a)或(b)中定义的多肽的DNA片段和以下(c)或(d)中定义的多肽:(a)至少具有氨基酸的氨基酸序列的多肽 序列表所示的SEQ ID NO:2中的酸值为50〜393,(b)至少具有序列表所示的SEQ ID No:2的氨基酸序列号为50〜393的氨基酸序列的多肽,包括取代, 一个或几个氨基酸的缺失,插入,添加或倒位,并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质,其具有氨基酸 - 磷酸合成酶的大亚基,其氨基酸序列至少包含氨基酸数55至 SEQ ID NO:3的1113,(c)具有至少包含序列表所示的SEQ ID NO:3的氨基酸编号55至1113的氨基酸序列的多肽,(d)具有氨基酸的多肽 序列至少包含氨基酸编号55至11 包括序列表中所示的SEQ ID NO:3的13个氨基酸残基,其包含一个或多个氨基酸的取代,缺失,插入,添加或倒位,并且可以构成具有氨甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基 具有SEQ ID NO:2中氨基酸序列号50至393的氨基酸序列。