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    • 3. 发明授权
    • DNA separation device, DNA separation method, and ligand DNA
    • DNA分离装置,DNA分离方法和配体DNA
    • US07887690B2
    • 2011-02-15
    • US11585939
    • 2006-10-25
    • Miho HayashiKazuyoshi MoriMizuo Maeda
    • Miho HayashiKazuyoshi MoriMizuo Maeda
    • G01N27/26G01N33/48
    • G01N27/447C12N15/101C12Q1/6827C12Q1/6837Y10T436/143333C12Q2565/125C12Q2535/131C12Q2525/161
    • A ligand DNA including a probe portion having a sequence that is complementary to a target DNA to be detected in a sample DNA, and a probe portion having a base sequence that is complementary to a base sequence of a marker DNA 2 in a conjugate DNA 10 is formed, and the sample DNA and the ligand DNA are combined to form a DNA complex, and further, the DNA complex is made to perform electrophoresis in the conjugate DNA. Therefore, it is possible to provide a DNA separation detection method which can appropriately separate the sample DNA and deal with various kinds of target sample DNAs in short time, without the necessity of searching for an optimum electrophoresis condition by complicatedly combining the amount of a bonding control agent to be contained in the conjugate DNA, the viscosity of linear polymer, the amount of the sample DNA, the amount of the conjugate DNA, and the like, for each DNA sequence as a detection target, when separating the sample DNA by electrophoresis.
    • 包含具有与样品DNA中待检测的靶DNA互补的序列的探针部分的配体DNA和具有与缀合DNA 10中的标记DNA 2的碱基序列互补的碱基序列的探针部分 ,并将样品DNA和配体DNA合并形成DNA复合物,进一步使DNA复合物在缀合物DNA中进行电泳。 因此,可以提供一种DNA分离检测方法,其可以在短时间内适当地分离样品DNA并处理各种目标样品DNA,而不需要通过将结合量复杂地组合来搜索最佳电泳条件 对于作为检测对象的每个DNA序列,当通过电泳分离样品DNA时,共轭DNA中包含的控制剂,线性聚合物的粘度,样品DNA的量,缀合物DNA的量等 。
    • 4. 发明申请
    • DNA separation device, DNA separation method, and ligand DNA
    • DNA分离装置,DNA分离方法和配体DNA
    • US20070138013A1
    • 2007-06-21
    • US11585939
    • 2006-10-25
    • Miho HayashiKazuyoshi MoriMizuo Maeda
    • Miho HayashiKazuyoshi MoriMizuo Maeda
    • C07K1/26G01N27/00
    • G01N27/447C12N15/101C12Q1/6827C12Q1/6837Y10T436/143333C12Q2565/125C12Q2535/131C12Q2525/161
    • A ligand DNA including a probe portion having a sequence that is complementary to a target DNA to be detected in a sample DNA, and a probe portion having a base sequence that is complementary to a base sequence of a marker DNA 2 in a conjugate DNA 10 is formed, and the sample DNA and the ligand DNA are combined to form a DNA complex, and further, the DNA complex is made to perform electrophoresis in the conjugate DNA. Therefore, it is possible to provide a DNA separation detection method which can appropriately separate the sample DNA and deal with various kinds of target sample DNAs in short time, without the necessity of searching for an optimum electrophoresis condition by complicatedly combining the amount of a bonding control agent to be contained in the conjugate DNA, the viscosity of linear polymer, the amount of the sample DNA, the amount of the conjugate DNA, and the like, for each DNA sequence as a detection target, when separating the sample DNA by electrophoresis.
    • 包含具有与样品DNA中待检测的靶DNA互补的序列的探针部分的配体DNA和具有与缀合DNA 10中的标记DNA 2的碱基序列互补的碱基序列的探针部分 ,并将样品DNA和配体DNA合并形成DNA复合物,进一步使DNA复合物在缀合物DNA中进行电泳。 因此,可以提供一种DNA分离检测方法,其可以在短时间内适当地分离样品DNA并处理各种目标样品DNA,而不需要通过将结合量复杂地组合来搜索最佳电泳条件 对于作为检测对象的每个DNA序列,当通过电泳分离样品DNA时,共轭DNA中包含的控制剂,线性聚合物的粘度,样品DNA的量,缀合物DNA的量等 。
    • 5. 发明申请
    • METHOD FOR AMPLIFYING A TARGET SEQUENCE INCLUDED IN A DOUBLE-STRANDED DNA
    • 用于扩增包含在双链DNA中的靶序列的方法
    • US20120322111A1
    • 2012-12-20
    • US13591918
    • 2012-08-22
    • Miho HayashiHidenobu Yaku
    • Miho HayashiHidenobu Yaku
    • C12P19/34
    • C12Q1/686C12Q2525/113C12Q2525/186
    • the present invention provides an amplification method capable of inhibiting the generating of the undesired amplified double-stranded DNA sequence. In the present method, DNA polymerase, deoxynucleoside triphosphate, the double-stranded DNA, a forward primer, a reverse primer, and a first block nucleic acid are mixed so as to amplify the double-stranded target sequence with use of a polymerase chain reaction. The first block nucleic acid does not serve as an origin for the elongation reaction with the DNA polymerase. The first block nucleic acid is complementary with a part of the third non-amplified sequence which is interposed between the 5′ end and the complimentary single-stranded target sequence. Due to the first block nucleic acid, the generating of the undesired amplified double-stranded DNA sequence is inhibited.
    • 本发明提供能够抑制不想要的扩增双链DNA序列的产生的扩增方法。 在本方法中,混合DNA聚合酶,脱氧核苷三磷酸,双链DNA,正向引物,反向引物和第一嵌段核酸,以便使用聚合酶链反应扩增双链靶序列 。 第一嵌段核酸不作为与DNA聚合酶的延伸反应的起始点。 第一嵌段核酸与介于5'末端和互补单链靶序列之间的第三个非扩增序列的一部分互补。 由于第一嵌段核酸,不希望的扩增双链DNA序列的产生被抑制。
    • 7. 发明授权
    • Laundry system including home terminal device and laundry apparatus with communicating function
    • 洗衣系统,包括家用终端设备和具有通讯功能的洗衣设备
    • US07174227B2
    • 2007-02-06
    • US10501900
    • 2002-12-27
    • Midori KobayashiHiroyuki HarunaFumihiro ImamuraYoshimi NishimuraMiho Hayashi
    • Midori KobayashiHiroyuki HarunaFumihiro ImamuraYoshimi NishimuraMiho Hayashi
    • G05B15/00
    • D06F39/005D06F33/02F24C7/085F24C7/086
    • A laundry system includes a home terminal device and laundry apparatus. The terminal device includes a first short-range communication device, a long-range communication device, and a terminal device controller transmitting information about laundry and a laundry constraint to a server connected to an Internet, thereby obtaining an operation control program for the laundry apparatus. The terminal device controller transfers the program via the first short-range communication device to the laundry apparatus and receives via the first short-range communication device information about an operating state of the laundry apparatus. The laundry apparatus includes a second short-range communication device, a laundry apparatus controller, and a laundry unit. The controller receives the program via the second short-range communication device from the terminal device to control the laundry unit and transfers the information about the operating state of the laundry apparatus via the second short-range communication device to the terminal device.
    • 洗衣系统包括家庭终端设备和洗衣设备。 终端装置包括:第一短距离通信装置,远程通信装置,以及向与因特网连接的服务器发送关于洗衣和洗衣约束的信息的终端装置控制器,从而获得洗衣装置的操作控制程序 。 终端设备控制器经由第一短距离通信设备将程序传送到洗衣设备,并且经由第一短距离通信设备接收关于洗衣设备的操作状态的信息。 洗衣设备包括第二短距离通信设备,洗衣设备控制器和洗衣单元。 所述控制器经由所述第二短距离通信装置从所述终端装置接收所述程序,以控制所述衣物单元,并且经由所述第二短距离通信装置将关于所述衣物装置的操作状态的信息传送到所述终端装置。
    • 8. 发明申请
    • Laundry system including home terminal device and laundry apparatus with communicating function
    • 洗衣系统,包括家用终端设备和具有通讯功能的洗衣设备
    • US20050109070A1
    • 2005-05-26
    • US10501900
    • 2002-12-27
    • Midori KobayashiHiroyuki HarunaFumihiro ImamuraYoshimi NishimuraMiho Hayashi
    • Midori KobayashiHiroyuki HarunaFumihiro ImamuraYoshimi NishimuraMiho Hayashi
    • D06F33/00D06F33/02D06F39/00H04Q9/00
    • D06F39/005D06F33/02F24C7/085F24C7/086
    • A laundry system (1) includes a home terminal device (3) and laundry apparatus (4). The terminal device includes a first short-range communication device (8), a long-range communication device (7), and a terminal device controller (9) transmitting information about laundry and information about a laundry constraint to a server (2) connected to an Internet (5), thereby obtaining an operation control program for the laundry apparatus. The terminal device controller transfers the obtained operation control program via the first short-range communication device to the laundry apparatus and receives via the first short-range communication device information about an operating state of the laundry apparatus. The laundry apparatus includes a second short-range communication device (15), a laundry apparatus controller (17), and a laundry unit (16). The laundry apparatus controller receives the operation control program via the second short-range communication device from the terminal device to control the laundry unit and transfers the information about the operating state of the laundry apparatus via the second short-range communication device to the terminal device.
    • 洗衣系统(1)包括家用终端设备(3)和洗衣设备(4)。 终端设备包括第一短距离通信设备(8),远距离通信设备(7)和终端设备控制器(9),其将关于洗衣的信息和关于衣物约束的信息传送到连接的服务器(2) (5),从而获得洗衣设备的操作控制程序。 终端设备控制器经由第一短距离通信设备将所获得的操作控制程序传送到洗衣设备,并经由第一短距离通信设备接收关于洗衣设备的操作状态的信息。 洗衣设备包括第二短距离通信设备(15),洗衣设备控制器(17)和洗衣单元(16)。 洗衣设备控制器经由第二短距离通信设备从终端设备接收操作控制程序以控制衣物单元,并且经由第二短距离通信设备将关于洗衣设备的操作状态的信息传送到终端设备 。