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1. 发明授权

US07579172B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07579172B2
公开(公告)日：2009-08-25
申请号：US11071383
申请日：2005-03-03
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/66 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： An apparatus for amplifying nucleic acids includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate, wherein the reactant aqueous solution contacts the fluid to create a plurality of droplets surrounded by the fluid in the reaction vessel and the nucleic acids are amplified in the reactant aqueous solution droplets.
摘要翻译：用于放大核酸的装置包括：基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许引入含有反应物的反应物水溶液，核酸扩增到反应容器中，形成在基底内的第二入口通道，连接到反应容器的末端，并允许引入与反应物水溶液相分离并且不参与扩增反应的流体反应容器和加热单元，其以与基底热接触并加热基底的方式安装在基底上，其中反应物水溶液接触流体以产生由反应容器中的流体包围的多个液滴，核酸在反应物水溶液液滴中被扩增。

2. 发明申请

US20050202489A1 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US20050202489A1
公开(公告)日：2005-09-15
申请号：US11071383
申请日：2005-03-03
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12N15/09 , B01F5/06 , B01F13/00 , B01F15/02 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/38 , C12N15/00 , C12Q1/68 , G01N35/00 , G01N35/08 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： Provided are a method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：提供了用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

3. 发明授权

US07943348B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07943348B2
公开(公告)日：2011-05-17
申请号：US12504271
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

4. 发明授权

US07915013B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07915013B2
公开(公告)日：2011-03-29
申请号：US12504213
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： An apparatus for amplifying nucleic acids. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：扩增核酸的装置。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

5. 发明申请

US20100021972A1 METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS 有权
标题翻译：用于放大核酸的方法和装置
公开(公告)号：US20100021972A1
公开(公告)日：2010-01-28
申请号：US12504271
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

6. 发明申请

US20050199917A1 Sensitivity enhanced biomolecule field effect transistor 有权
标题翻译：灵敏度增强的生物分子场效应晶体管
公开(公告)号：US20050199917A1
公开(公告)日：2005-09-15
申请号：US11057642
申请日：2005-02-14
申请人： Kyu-tae Yoo , Geun-bae Lim , Joon-ho Kim , Kak Namkoong
发明人： Kyu-tae Yoo , Geun-bae Lim , Joon-ho Kim , Kak Namkoong
IPC分类号： G01N27/327 , C12Q1/68 , G01N27/414 , G01N33/487 , H01L29/08
CPC分类号： G01N27/4145
摘要： There is provided a biomolecule FET enhancing a sensitivity. The biomolecule FET includes a substrate, first and second impurity regions formed on both sides of the substrate, and doped with impurities of a polarity opposite to that of the substrate, a gate formed on the substrate and being in contact with the first and second impurity regions, and a probe biomolecule attached to the gate. A region of the gate adjacent to the first impurity region is wider than a region thereof adjacent to the second impurity region. A density of the probe biomolecule attached to the surface of the gate is increased, and when detecting a level of hybridization of the probe biomolecule and the target biomolecule, its sensitivity is improved.
摘要翻译：提供了提高灵敏度的生物分子FET。生物分子FET包括基板，形成在基板两侧的第一和第二杂质区，并且掺杂极性与基板极性相反的杂质;形成在基板上并与第一和第二杂质接触的栅极区域，以及连接到门的探针生物分子。与第一杂质区相邻的栅极的区域比与第二杂质区相邻的区域宽。附着在栅极表面的探针生物分子的密度增加，并且当检测到探针生物分子和靶生物分子的杂交水平时，其灵敏度得到改善。

7. 发明授权

US07151301B2 Sensitivity enhanced biomolecule field effect transistor 有权
标题翻译：灵敏度增强的生物分子场效应晶体管
公开(公告)号：US07151301B2
公开(公告)日：2006-12-19
申请号：US11057642
申请日：2005-02-14
申请人： Kyu-tae Yoo , Geun-bae Lim , Joon-ho Kim , Kak Namkoong
发明人： Kyu-tae Yoo , Geun-bae Lim , Joon-ho Kim , Kak Namkoong
IPC分类号： H01L29/76
CPC分类号： G01N27/4145
摘要： There is provided a biomolecule FET enhancing a sensitivity. The biomolecule FET includes a substrate, first and second impurity regions formed on both sides of the substrate, and doped with impurities of a polarity opposite to that of the substrate, a gate formed on the substrate and being in contact with the first and second impurity regions, and a probe biomolecule attached to the gate. A region of the gate adjacent to the first impurity region is wider than a region thereof adjacent to the second impurity region. A density of the probe biomolecule attached to the surface of the gate is increased, and when detecting a level of hybridization of the probe biomolecule and the target biomolecule, its sensitivity is improved.
摘要翻译：提供了提高灵敏度的生物分子FET。生物分子FET包括基板，形成在基板两侧的第一和第二杂质区，并且掺杂极性与基板极性相反的杂质;形成在基板上并与第一和第二杂质接触的栅极区域，以及连接到门的探针生物分子。与第一杂质区相邻的栅极的区域比与第二杂质区相邻的区域宽。附着在栅极表面的探针生物分子的密度增加，并且当检测到探针生物分子和靶生物分子的杂交水平时，其灵敏度得到改善。

8. 发明授权

US07790440B2 Sensing switch and detecting method using the same 有权
标题翻译：感应开关及使用其的检测方法
公开(公告)号：US07790440B2
公开(公告)日：2010-09-07
申请号：US12272183
申请日：2008-11-17
申请人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
发明人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
IPC分类号： C12M3/00
CPC分类号： G01N33/54373
摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.
摘要翻译：提供了一种感测开关和使用该感测开关的感测方法。感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

9. 发明授权

US07510865B2 Sensing switch and detecting method using the same 失效
标题翻译：感应开关及使用其的检测方法
公开(公告)号：US07510865B2
公开(公告)日：2009-03-31
申请号：US11347185
申请日：2006-02-03
申请人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
发明人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
IPC分类号： C12M1/34
CPC分类号： G01N33/54373
摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.
摘要翻译：提供了一种感测开关和使用该感测开关的感测方法。感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

10. 发明授权

US07981666B2 Sensing switch and detecting method using the same 有权
标题翻译：感应开关及使用其的检测方法
公开(公告)号：US07981666B2
公开(公告)日：2011-07-19
申请号：US12833095
申请日：2010-07-09
申请人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
发明人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
IPC分类号： C12M3/00
CPC分类号： G01N33/54373
摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.
摘要翻译：提供了一种感测开关和使用该感测开关的感测方法。感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

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