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1. 发明授权

US08409841B2 Hydroxynitrile lyase 失效
公开(公告)号：US08409841B2
公开(公告)日：2013-04-02
申请号：US13210630
申请日：2011-08-16
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/00 , C12N9/88 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

2. 发明授权

US08030053B2 Substitutional variants of hydroxynitrile lyase with high specific activity and methods of use 失效
标题翻译：具有高比活性和使用方法的羟基腈裂解酶的替代变体
公开(公告)号：US08030053B2
公开(公告)日：2011-10-04
申请号：US11665491
申请日：2005-10-14
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/00 , C12N1/20 , C12N15/00 , C07H21/02
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
摘要翻译：改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。

3. 发明申请

US20090170156A1 Hydroxynitrile lyase 失效
标题翻译：羟基腈裂解酶
公开(公告)号：US20090170156A1
公开(公告)日：2009-07-02
申请号：US11665491
申请日：2005-10-14
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12P21/00 , C12N9/88 , C12N15/74 , C12N5/10 , C12P13/00
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
摘要翻译：改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。

4. 发明授权

US08383389B2 Hydroxynitrile lyase 失效
公开(公告)号：US08383389B2
公开(公告)日：2013-02-26
申请号：US13210646
申请日：2011-08-16
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/00 , C12N9/88 , C12N1/20 , C12N15/00
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

5. 发明申请

US20120040417A1 HYDROXYNITRILE LYASE 失效
标题翻译：羟肟酸钠
公开(公告)号：US20120040417A1
公开(公告)日：2012-02-16
申请号：US13210646
申请日：2011-08-16
申请人： Yasuhisa ASANO , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa ASANO , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12P13/00 , C12N9/88 , C12N5/10 , C12N15/60 , C12N1/15 , C12N1/19 , C12N15/63 , C12P7/42 , C12N1/21
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
摘要翻译：改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。

6. 发明申请

US20120028317A1 HYDROXYNITRILE LYASE 失效
公开(公告)号：US20120028317A1
公开(公告)日：2012-02-02
申请号：US13210630
申请日：2011-08-16
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/88 , C12P7/40 , C12N15/60 , C12N5/10 , C12N1/21 , C12N1/19 , C12N1/15 , C12P13/00 , C12N15/63
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

7. 发明申请

US20100136617A1 HALOHYDRIN EPOXIDASE 失效
标题翻译：盐酸环氧化酶
公开(公告)号：US20100136617A1
公开(公告)日：2010-06-03
申请号：US12529657
申请日：2008-03-07
申请人： Takanori Akiyama , Fujio Yu , Fumiaki Watanabe , Yuya Takikawa , Eiji Sato
发明人： Takanori Akiyama , Fujio Yu , Fumiaki Watanabe , Yuya Takikawa , Eiji Sato
IPC分类号： C12P21/06 , C12N9/88 , C07H21/04 , C12N15/63 , C12N5/10 , C12P17/02 , C12P13/00
CPC分类号： C12P13/004 , C12N9/0071
摘要： The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.
摘要翻译：本发明提供工业上有用的改进的卤代醇环氧酶，其制备方法，以及使用其制备表卤代醇或4-卤代-3-羟基丁腈的方法。本发明的改进的卤代醇环氧酶由将特定氨基酸取代突变引入包含预定氨基酸序列I和II的野生型卤代醇环氧酶的氨基酸序列中的氨基酸序列组成。

8. 发明授权

US08637272B2 Halohydrin epoxidase 失效
标题翻译：卤代醇环氧酶
公开(公告)号：US08637272B2
公开(公告)日：2014-01-28
申请号：US12529657
申请日：2008-03-07
申请人： Takanori Akiyama , Yu Fujio , Fumiaki Watanabe , Yuya Takikawa , Eiji Sato
发明人： Takanori Akiyama , Yu Fujio , Fumiaki Watanabe , Yuya Takikawa , Eiji Sato
IPC分类号： C12P21/06 , C12N9/00 , C12N9/88 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P13/004 , C12N9/0071
摘要： The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.
摘要翻译：本发明提供工业上有用的改进的卤代醇环氧酶，其制备方法，以及使用其制备表卤代醇或4-卤代-3-羟基丁腈的方法。本发明的改进的卤代醇环氧酶由将特定氨基酸取代突变引入包含预定氨基酸序列I和II的野生型卤代醇环氧酶的氨基酸序列中的氨基酸序列组成。

9. 发明授权

US09470230B2 Refrigerant compressor and refrigeration cycle apparatus using the same 有权
标题翻译：制冷剂压缩机及使用其的制冷循环装置
公开(公告)号：US09470230B2
公开(公告)日：2016-10-18
申请号：US14110613
申请日：2011-04-25
申请人： Masaki Koyama , Eiji Sato
发明人： Masaki Koyama , Eiji Sato
IPC分类号： F04C18/02 , F04C29/02 , F04C15/00 , F04C23/00 , F04B39/02 , F04C28/08 , F25B31/00 , F25B49/02 , F04C29/04 , F25B13/00
CPC分类号： F04C29/021 , F04B39/0207 , F04B39/0261 , F04B2203/0209 , F04C15/0092 , F04C18/0215 , F04C23/008 , F04C28/08 , F04C29/028 , F04C29/04 , F04C2240/806 , F04C2270/052 , F25B13/00 , F25B31/004 , F25B49/022 , F25B2600/021 , F25B2700/171 , F25B2700/1931 , F25B2700/1933 , Y02B30/741
摘要： A refrigerant compressor includes a sealed case, which contains a compression mechanism, a motor part, and an oil reservoir formed in a lower portion. An oil supply pipe links the oil reservoir and a suction side of the compression mechanism and introduces oil in the oil reservoir to the suction side. The amount of oil supply provided to the suction side is adjusted by detecting the rotational speed of the motor part, such that the amount of oil supply provided to the suction side of the oil supply pipe decreases as the rotational speed of the motor part increases.
摘要翻译：制冷剂压缩机包括密封壳体，其包含压缩机构，电动机部件和形成在下部的储油器。供油管将储油器和压缩机构的吸入侧连接，并将油储存器中的油引入吸入侧。通过检测电动机部件的转速来调整供给到吸入侧的供油量，使得供给管的吸入侧的供油量随着电动机部的转速的增加而减小。

10. 发明申请

US20130181349A1 SEMICONDUCTOR DEVICE HAVING THROUGH-SUBSTRATE VIA 审中-公开
标题翻译：具有通过基底的半导体器件
公开(公告)号：US20130181349A1
公开(公告)日：2013-07-18
申请号：US13599041
申请日：2012-08-30
申请人： Chie KOYAMA , Satoyuki Miyako , Eiji Sato
发明人： Chie KOYAMA , Satoyuki Miyako , Eiji Sato
IPC分类号： H01L23/498
CPC分类号： H01L23/481 , H01L21/76224 , H01L23/5225 , H01L23/5286 , H01L27/14618 , H01L27/14636 , H01L2924/0002 , H01L2924/00
摘要： According to an embodiment, a semiconductor device includes a first circuit block, a first through-substrate via, and a back surface wiring. The first circuit block is provided on a surface side of a semiconductor substrate. The first through-substrate via is provided along a circumference of the first circuit block so as to separate the first circuit block from other circuit blocks. The first circuit block is provided so as to penetrate the surface of the semiconductor substrate. The first circuit block is isolated from the surroundings. The first circuit block has conductivity. The back surface wiring is provided on the back surface side of the semiconductor substrate. The back surface wiring is connected to the first through-substrate via. The back surface wiring connects the first through-substrate via to a power supply terminal or a shield potential terminal.
摘要翻译：根据实施例，半导体器件包括第一电路块，第一贯穿衬底通孔和背面布线。第一电路块设置在半导体衬底的表面侧。沿着第一电路块的圆周设置第一贯穿衬底通孔，以将第一电路块与其它电路块分离。第一电路块被设置为穿透半导体衬底的表面。第一个电路块与周围环境隔绝。第一个电路块具有导电性。背面布线设置在半导体基板的背面侧。背面布线连接到第一贯穿基板通孔。背面布线将第一贯穿基板通孔连接到电源端子或屏蔽电位端子。

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