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1. 发明授权

US08383389B2 Hydroxynitrile lyase 失效
公开(公告)号：US08383389B2
公开(公告)日：2013-02-26
申请号：US13210646
申请日：2011-08-16
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/00 , C12N9/88 , C12N1/20 , C12N15/00
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

2. 发明授权

US08409841B2 Hydroxynitrile lyase 失效
公开(公告)号：US08409841B2
公开(公告)日：2013-04-02
申请号：US13210630
申请日：2011-08-16
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/00 , C12N9/88 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

3. 发明申请

US20120040417A1 HYDROXYNITRILE LYASE 失效
标题翻译：羟肟酸钠
公开(公告)号：US20120040417A1
公开(公告)日：2012-02-16
申请号：US13210646
申请日：2011-08-16
申请人： Yasuhisa ASANO , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa ASANO , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12P13/00 , C12N9/88 , C12N5/10 , C12N15/60 , C12N1/15 , C12N1/19 , C12N15/63 , C12P7/42 , C12N1/21
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
摘要翻译：改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。

4. 发明申请

US20120028317A1 HYDROXYNITRILE LYASE 失效
公开(公告)号：US20120028317A1
公开(公告)日：2012-02-02
申请号：US13210630
申请日：2011-08-16
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/88 , C12P7/40 , C12N15/60 , C12N5/10 , C12N1/21 , C12N1/19 , C12N1/15 , C12P13/00 , C12N15/63
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

5. 发明授权

US08030053B2 Substitutional variants of hydroxynitrile lyase with high specific activity and methods of use 失效
标题翻译：具有高比活性和使用方法的羟基腈裂解酶的替代变体
公开(公告)号：US08030053B2
公开(公告)日：2011-10-04
申请号：US11665491
申请日：2005-10-14
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/00 , C12N1/20 , C12N15/00 , C07H21/02
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
摘要翻译：改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。

6. 发明申请

US20090170156A1 Hydroxynitrile lyase 失效
标题翻译：羟基腈裂解酶
公开(公告)号：US20090170156A1
公开(公告)日：2009-07-02
申请号：US11665491
申请日：2005-10-14
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12P21/00 , C12N9/88 , C12N15/74 , C12N5/10 , C12P13/00
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
摘要翻译：改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。

7. 发明申请

US20100136617A1 HALOHYDRIN EPOXIDASE 失效
标题翻译：盐酸环氧化酶
公开(公告)号：US20100136617A1
公开(公告)日：2010-06-03
申请号：US12529657
申请日：2008-03-07
申请人： Takanori Akiyama , Fujio Yu , Fumiaki Watanabe , Yuya Takikawa , Eiji Sato
发明人： Takanori Akiyama , Fujio Yu , Fumiaki Watanabe , Yuya Takikawa , Eiji Sato
IPC分类号： C12P21/06 , C12N9/88 , C07H21/04 , C12N15/63 , C12N5/10 , C12P17/02 , C12P13/00
CPC分类号： C12P13/004 , C12N9/0071
摘要： The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.
摘要翻译：本发明提供工业上有用的改进的卤代醇环氧酶，其制备方法，以及使用其制备表卤代醇或4-卤代-3-羟基丁腈的方法。本发明的改进的卤代醇环氧酶由将特定氨基酸取代突变引入包含预定氨基酸序列I和II的野生型卤代醇环氧酶的氨基酸序列中的氨基酸序列组成。

8. 发明授权

US07557202B2 DNA fragments containing gene having function relating to autonomous proliferation of plasmid 有权
标题翻译：含有与质粒自主增殖有关的功能基因的DNA片段
公开(公告)号：US07557202B2
公开(公告)日：2009-07-07
申请号：US10482156
申请日：2002-07-03
申请人： Wataru Mizunashi , Fujio Yu
发明人： Wataru Mizunashi , Fujio Yu
IPC分类号： C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C07K14/195 , C12N15/69
摘要： Provided are a DNA fragment containing a gene relating the autonomous replication of a plasmid, and a DNA fragment containing a gene relating to the autonomous replication of a plasmid wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site in the gene. Also provided are a DNA fragment containing a gene having a function relating to the autonomous replication of a plasmid within the bacteria of the genus Rhodococcus, a DNA fragment wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site in the gene, and a plasmid carrying a DNA fragment wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site of the gene.
摘要翻译：提供了含有与质粒自主复制有关的基因的DNA片段和含有与质粒自主复制有关的基因的DNA片段，其中能够增加质粒拷贝数的突变点至少存在基因中的一个位点。还提供了含有与红球菌属细菌中的质粒自主复制有关的功能的基因的DNA片段，其中至少存在能增加质粒拷贝数的突变点的DNA片段该基因的一个位点和携带DNA片段的质粒，其中能够增加质粒拷贝数的突变点存在于该基因的至少一个位点。

9. 发明授权

US5776771A Kanamycin resistance gene derived from microorganisms of the genus rhodococcus 失效
标题翻译：源自红球菌属微生物的卡那霉素抗性基因
公开(公告)号：US5776771A
公开(公告)日：1998-07-07
申请号：US510878
申请日：1995-08-03
申请人： Fujio Yu , Mami Kato
发明人： Fujio Yu , Mami Kato
IPC分类号： C12N15/09 , C07K14/195 , C07K14/41 , C12N1/21 , C12N9/00 , C12N15/65 , C12N15/74 , C12P21/02 , C12R1/01 , C12R1/19 , C12N15/31 , C12N15/70
CPC分类号： C12N15/65 , C07K14/195 , C12N15/74 , C12N9/00
摘要： The present invention relates to a DNA derived from microorganisms of the genus Rhodococcus and conferring kanamycin resistance on hosts with a DNA sequence coding for the amino acid sequence of Sequence No. 1 or a polypeptide containing a partial sequence thereof. The kanamycin resistance gene of the present invention is useful to construct vectors for microorganisms of the genus Rhodococcus, particularly vectors for self-cloning of Rhodococcus rhodochrous.
摘要翻译：本发明涉及一种衍生自红球菌属微生物的DNA，并赋予具有编码序列号1的氨基酸序列的DNA序列的宿主的卡那霉素抗性或含有其部分序列的多肽。本发明的卡那霉素抗性基因可用于构建红球菌属微生物的载体，特别是用于玫瑰色红球菌自身克隆的载体。

10. 发明授权

US5602014A Regulatory system for expression of nitrilase gene 失效
标题翻译：腈水解酶基因表达调控系统
公开(公告)号：US5602014A
公开(公告)日：1997-02-11
申请号：US577184
申请日：1995-12-22
申请人： Yurie Mizumura , Fujio Yu
发明人： Yurie Mizumura , Fujio Yu
IPC分类号： C12N15/09 , C07K14/195 , C12N1/21 , C12N9/78 , C12N15/31 , C12N15/74 , C12R1/01 , C12N15/00 , C12P21/00
CPC分类号： C12N9/78 , C07K14/195 , C12N15/74
摘要： The invention relates to a regulatory factor or system composed of 2 components of a polypeptide having the amino acid sequence of SEQ ID No:1 and a polypeptide having the amino acid sequence of SEQ ID No:2 to activate a nitrilase gene promoter, as well as to DNA coding therefor. Nitrilase can be produced by introducing the gene coding for the present regulatory factor together with a nitrilase gene containing a promoter region into a microorganism of the genus Rhodococcus.
摘要翻译：本发明涉及由具有SEQ ID No：1的氨基酸序列的多肽的2种成分和具有SEQ ID No：2的氨基酸序列的多肽组成的调节因子或系统，以激活腈水解酶基因启动子关于DNA编码。可以通过将编码本调节因子的基因与含有启动子区域的腈水解酶基因导入红球菌属的微生物来生产腈水解酶。

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