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1. 发明授权

US09243275B1 Biosensor and method of making same 有权
公开(公告)号：US09243275B1
公开(公告)日：2016-01-26
申请号：US10888342
申请日：2004-07-09
申请人： Kalle Levon , Bin Yu , Yanxiu Zhou
发明人： Kalle Levon , Bin Yu , Yanxiu Zhou
IPC分类号： C12Q1/00 , C07K9/00 , C07K14/32 , C07K17/00 , G01N33/53
CPC分类号： C12Q1/00 , C07K9/00 , C07K14/32 , C07K17/00 , C12Q1/003 , G01N33/53 , G01N33/54353 , G01N33/5438 , G01N33/54393 , G01N33/552
摘要： Sensors suitable for the sensing/detection of biological or chemical agents may be fabricated by immobilizing biological and/or chemical recognition components (selectors or probes) on a substrate by the polymerization of a suitable monomer in the presence of the selectors or probes, for example, by Polysiloxane Monolayer Immobilization (PMI). PMI may involve the polymerization of polysiloxane onto a substrate, onto which selector molecules are adsorbed or otherwise immobilized. The resulting immobilized selector molecule may then be used to interact with specific molecules (targets) within a mixture of molecules, thereby enabling those specific molecules to be detected and/or quantified.

2. 发明申请

US20090061464A1 METHODS FOR DETECTING AND/OR QUANTIFYING A CONCENTRATION OF SPECIFIC BACTERIAL MOLECULES USING BACTERIAL BIOSENSORS 失效
标题翻译：使用细菌生物传感器检测和/或定量特异性细菌分子的浓度的方法
公开(公告)号：US20090061464A1
公开(公告)日：2009-03-05
申请号：US12176942
申请日：2008-07-21
申请人： Kalle LEVON , Bin Yu , Yanxiu Zhou
发明人： Kalle LEVON , Bin Yu , Yanxiu Zhou
IPC分类号： G01N33/554
CPC分类号： C12Q1/00 , C07K9/00 , C07K14/32 , C07K17/00 , C12Q1/003 , G01N33/53 , G01N33/54353 , G01N33/5438 , G01N33/54393 , G01N33/552
摘要： A real-time method employing a portable peptide-containing potentiometric biosensor, can directly detect and/or quantify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B. subtilis spores in a concentration range of 0.08-7.3×104 CFU/ml. The sensing method exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10−5. The biosensor method not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensing method can distinguish a Bacillus subtilis 1A700 from other B. subtilis strain. Assay time may be as low as about 5 minutes for a single test. Rapid identification of B. anthracis Sterne and B. anthracis ΔAmes was also provided.
摘要翻译：采用便携式含肽电位生物传感器的实时方法可以直接检测和/或定量细菌孢子。用于特异性识别枯草芽孢杆菌和炭疽芽孢杆菌的两种肽可以通过聚硅氧烷单层固定（PMI）技术来固定。传感器通过检测例如浓度范围为0.08-7.3×10 4 CFU / ml的枯草芽孢杆菌孢子将生物识别事件转化为潜在的变化。该感测方法在其他种类的孢子上表现出对枯草芽孢杆菌孢子的高选择性识别性能。其他类型孢子的传感器的选择性系数在0-1.0x10-5的范围内。生物传感器方法不仅具有区分枯草芽孢杆菌和苏云金芽孢杆菌（Thur。）Kurstaki孢子的混合物中的枯草芽孢杆菌孢子的特异性，而且可以区分活枯枯病芽孢杆菌孢子和死枯病芽孢杆菌孢子。此外，感测方法可以将枯草芽孢杆菌1A700与其他枯草芽孢杆菌菌株区分开。单次测试的测定时间可能低至约5分钟。炭疽杆菌和炭疽杆菌的快速鉴定也提供了DeltaAmes。

3. 发明申请

US20050272105A1 Bacterial biosensors 审中-公开
标题翻译：细菌生物传感器
公开(公告)号：US20050272105A1
公开(公告)日：2005-12-08
申请号：US10888530
申请日：2004-07-09
申请人： Kalle Levon , Bin Yu , Yanxiu Zhou
发明人： Kalle Levon , Bin Yu , Yanxiu Zhou
IPC分类号： A61F13/15 , C12M1/34 , C12Q1/00 , G01N20060101 , G01N33/543 , G01N33/552 , G01N33/554 , G01N33/569
CPC分类号： C12Q1/00 , C07K9/00 , C07K14/32 , C07K17/00 , C12Q1/003 , G01N33/53 , G01N33/54353 , G01N33/5438 , G01N33/54393 , G01N33/552
摘要： A real-time, portable peptide-containing potentiometric biosensor that can directly identify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B. subtilis spores in a concentration range of 0.08-7.3×104 CFU/ml. The sensor exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10−5. The biosensor system not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensor can distinguish a Bacillus subtilis 1A700 from other B. subtilis strain. Assay time may be as low as about 5 minutes for a single test. Rapid identification of B. anthracis Sterne and B. anthracis ΔAmes was also provided.
摘要翻译：一种可直接鉴定细菌孢子的实时便携式含肽电位生物传感器。用于特异性识别枯草芽孢杆菌和炭疽芽孢杆菌的两种肽可以通过聚硅氧烷单层固定（PMI）技术来固定。传感器通过检测例如浓度范围为0.08-7.3×10 4 CFU / ml的枯草芽孢杆菌孢子将生物识别事件转化为潜在的变化。传感器表现出对枯草芽孢杆菌孢子与其他种类孢子的高选择性识别性能。用于其他种类孢子的传感器的选择性系数在0-1.0×10 -5的范围内。生物传感器系统不仅具有将枯草芽孢杆菌和苏云金芽孢杆菌（thur。）Kurstaki孢子的混合物中的枯草芽孢杆菌孢子区分开的特异性，而且可以区分活枯枯病芽孢杆菌孢子和活枯草芽孢杆菌孢子。此外，传感器可以将枯草芽孢杆菌1A700与其他枯草芽孢杆菌菌株区分开来。单次测试的测定时间可能低至约5分钟。炭疽杆菌和炭疽杆菌的快速鉴定也提供了DeltaAmes。

4. 发明申请

US20160003813A1 Biosensor and method of making same 有权
标题翻译：生物传感器及其制作方法
公开(公告)号：US20160003813A1
公开(公告)日：2016-01-07
申请号：US10888342
申请日：2004-07-09
申请人： Kalle Levon , Bin Yu , Yanxiu Zhou
发明人： Kalle Levon , Bin Yu , Yanxiu Zhou
IPC分类号： G01N33/543 , C09D183/04 , C07K17/08 , G01N33/545 , G01N27/327
CPC分类号： C12Q1/00 , C07K9/00 , C07K14/32 , C07K17/00 , C12Q1/003 , G01N33/53 , G01N33/54353 , G01N33/5438 , G01N33/54393 , G01N33/552
摘要： Sensors suitable for the sensing/detection of biological or chemical agents may be fabricated by immobilizing biological and/or chemical recognition components (selectors or probes) on a substrate by the polymerization of a suitable monomer in the presence of the selectors or probes, for example, by Polysiloxane Monolayer Immobilization (PMI). PMI may involve the polymerization of polysiloxane onto a substrate, onto which selector molecules are adsorbed or otherwise immobilized. The resulting immobilized selector molecule may then be used to interact with specific molecules (targets) within a mixture of molecules, thereby enabling those specific molecules to be detected and/or quantified.
摘要翻译：适于感测/检测生物或化学试剂的传感器可以通过在选择器或探针的存在下通过合适的单体的聚合将生物和/或化学识别组分（选择器或探针）固定在基底上来制备，例如，通过聚硅氧烷单层固定（PMI）。 PMI可以涉及将聚硅氧烷聚合到底物上，在其上吸附或以其他方式固定选择分子。然后可以使得到的固定化选择分子与分子混合物中的特定分子（靶）相互作用，从而使得能够检测和/或定量那些特异性分子。

5. 发明授权

US07824539B2 Ionic based sensing for identifying genomic sequence variations and detecting mismatch base pairs, such as single nucleotide polymorphisms 失效
标题翻译：用于鉴定基因组序列变异和检测错配碱基对（例如单核苷酸多态性）的基于离子的感测
公开(公告)号：US07824539B2
公开(公告)日：2010-11-02
申请号：US11090944
申请日：2005-03-25
申请人： Yanxiu Zhou , Bin Yu , Kalle Levon
发明人： Yanxiu Zhou , Bin Yu , Kalle Levon
IPC分类号： G01N27/26
CPC分类号： C12Q1/6825 , C12Q2600/156 , G01N27/333 , C12Q2565/518
摘要： Ionic interactions are monitored to detect hybridization. The measurement may be done measuring the potential change in the solution with the ion sensitive electrode (which may be the conducting polymer (e.g., polyaniline) itself), without applying any external energy during the binding. The double helix formation during the complimentary hybridization makes this electrode act as an ion selective electrode—the nucleotide hydrogen bonding is specific and thus monitoring the ionic phosphate group addition becomes selective. Polyaniline on the surface of nylon film forms a positively charged polymer film. Thiol linkage can be utilized for polyaniline modification and thiol-modified single strand oligonucleotide chains can be added to polyaniline. The sensitivity is because the double helix formation during the complimentary hybridization makes this electrode act as an ion selective electrode as the nucleotide hydrogen bonding is specific and thus monitoring the ionic phosphate group addition becomes selective.
摘要翻译：监测离子相互作用以检测杂交。测量可以通过离子敏感电极（其可以是导电聚合物（例如聚苯胺）本身））测量溶液中的潜在变化，而在结合期间不施加任何外部能量。在互补杂交期间的双螺旋形成使得该电极用作离子选择性电极 - 核苷酸氢键是特异性的，因此监测离子性磷酸酯基添加成为选择性的。尼龙膜表面的聚苯胺形成带正电的聚合物膜。硫醇连接可用于聚苯胺改性，硫醇改性的单链寡核苷酸链可以加入到聚苯胺中。敏感性是因为互补杂交期间的双螺旋形成使得该电极作为离子选择性电极，因为核苷酸氢键是特异性的，因此监测离子性磷酸酯基添加成为选择性的。

6. 发明申请

US20080227651A1 Cyclic voltammetry (CV) for identifying genomic sequence variations and detecting mismatch base pairs, such as single nucleotide polymorphisms 审中-公开
标题翻译：用于鉴定基因组序列变异和检测错配碱基对（如单核苷酸多态性）的循环伏安法（CV）
公开(公告)号：US20080227651A1
公开(公告)日：2008-09-18
申请号：US11089436
申请日：2005-03-24
申请人： Yanxiu Zhou , Bin Yu , Kalle Levon
发明人： Yanxiu Zhou , Bin Yu , Kalle Levon
IPC分类号： C40B30/00 , C40B40/06 , C40B50/18
CPC分类号： C12Q1/6825 , B01J2219/00653 , B01J2219/00713 , B01J2219/00722 , C12Q1/6874 , G01N27/48 , C12Q2565/607 , C12Q2565/501
摘要： Cyclic voltammetry (CV) may be used with novel sensors for identifying the presence of target sequences complementary to probe sequences. The sensor may include an electrode layer (which is used as a working electrode in a CV system), a conductive polymer layer, and probes immobilized (e.g., via sulfur) on the conductive polymer layer. The conductive polymer layer may be polyaniline, or the like. The probes may be immobilized on the polymer layer using an electro-chemical immobilization technique in the presence of nucleophiles, such as thiol groups for example. The probes may be oligionucleotides. Thus, the sensors may be used for identifying genomic sequence variations and detecting mismatch base pairs, such as single nucleotide polymorphisms (SNPs) for example.
摘要翻译：循环伏安法（CV）可用于识别存在与探针序列互补的靶序列的新型传感器。传感器可以包括电极层（其用作CV系统中的工作电极），导电聚合物层和在导电聚合物层上固定（例如通过硫）的探针）。导电聚合物层可以是聚苯胺等。探针可以使用电化学固定技术在亲核试剂例如巯基的存在下固定在聚合物层上。探针可以是寡核苷酸。因此，传感器可用于鉴定基因组序列变异并检测错配碱基对，例如单核苷酸多态性（SNP）。

7. 发明授权

US06827840B2 Chiral ligand exchange potentiometry and enantioselective sensors 失效
标题翻译：手性配体交换电位法和对映选择性传感器
公开(公告)号：US06827840B2
公开(公告)日：2004-12-07
申请号：US10170903
申请日：2002-06-13
申请人： Kalle Levon , Bin Yu , Yanxiu Zhou
发明人： Kalle Levon , Bin Yu , Yanxiu Zhou
IPC分类号： G01N27327
CPC分类号： G01N27/3335
摘要： Enantiomeric resolution is realized by combining an electrochemical method with ligand exchange (LE) in a novel electrochemical method named chiral ligand exchange potentiometry. Chiral selector ligands preferentially recognize certain enantiomers and undergo ligand exchange with the enantiomeric labile coordination complexes to form diastereoisomeric complexes. These complexes can form in solution and be recognized by an unmodified electrode, or they can be immobilized on the surface of a modified electrode (chiral sensor) incorporated with the chiral selector ligand by polysiloxane monolayer immobilization (PMI). Considerable stereoselectivity occurs in the formation of these diastereoisomeric complexes, and their net charges (Nernst factors) are different, thus enabling enantiomers to be distinguished by potentiometric electrodes without any pre-separation processes.
摘要翻译：通过将电化学方法与配体交换（LE）结合在一种称为手性配体交换电位法的新型电化学方法中来实现对映体拆分。手性选择配体优先识别某些对映异构体并与对映异构体不稳定配位络合物进行配体交换以形成非对映异构体复合物。这些配合物可以在溶液中形成并被未改性的电极识别，或者它们可以通过聚硅氧烷单层固定化（PMI）固定在掺入手性选择配体的改性电极（手性传感器）的表面上。在形成这些非对映异构体复合物时会产生相当大的立体选择性，它们的净电荷（能斯特因子）是不同的，因此能够通过电位电极区分对映异构体，而无需任何预分离过程。

8. 发明授权

US08679806B2 Methods for detecting and/or quantifying a concentration of specific bacterial molecules using bacterial biosensors 失效
标题翻译：使用细菌生物传感器检测和/或定量特异性细菌分子的浓度的方法
公开(公告)号：US08679806B2
公开(公告)日：2014-03-25
申请号：US12176942
申请日：2008-07-21
申请人： Kalle Levon , Bin Yu , Yanxiu Zhou
发明人： Kalle Levon , Bin Yu , Yanxiu Zhou
IPC分类号： C12N13/00 , C12N11/00 , G01N33/53 , C12Q1/00
CPC分类号： C12Q1/00 , C07K9/00 , C07K14/32 , C07K17/00 , C12Q1/003 , G01N33/53 , G01N33/54353 , G01N33/5438 , G01N33/54393 , G01N33/552
摘要： A real-time method employing a portable peptide-containing potentiometric biosensor, can directly detect and/or quantify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B. subtilis spores in a concentration range of 0.08-7.3×104 CFU/ml. The sensing method exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10−5. The biosensor method not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensing method can distinguish a Bacillus subtilis 1A700 from other B. subtilis strain. Assay time may be as low as about 5 minutes for a single test. Rapid identification of B. anthracis Sterne and B. anthracis ΔAmes was also provided.
摘要翻译：采用便携式含肽电位生物传感器的实时方法可以直接检测和/或定量细菌孢子。用于特异性识别枯草芽孢杆菌和炭疽芽孢杆菌的两种肽可以通过聚硅氧烷单层固定（PMI）技术来固定。传感器通过检测例如浓度范围为0.08-7.3×104CFU / ml的枯草芽孢杆菌孢子将生物识别事件转化为潜在的变化。该感测方法在其他种类的孢子上表现出对枯草芽孢杆菌孢子的高度选择性识别性能。其他类型孢子传感器的选择性系数在0-1.0×10-5的范围内。生物传感器方法不仅具有区分枯草芽孢杆菌和苏云金芽孢杆菌（Thur。）Kurstaki孢子的混合物中的枯草芽孢杆菌孢子的特异性，而且可以区分活枯枯病芽孢杆菌孢子和死枯病芽孢杆菌孢子。此外，感测方法可以将枯草芽孢杆菌1A700与其他枯草芽孢杆菌菌株区分开。单次测试的测定时间可能低至约5分钟。还提供了炭疽杆菌和炭疽芽孢杆菌的快速鉴定。

9. 发明申请

US20080197025A1 Ionic based sensing for identifying genomic sequence variations and detecting mismatch base pairs, such as single nucleotide polymorphisms 失效
标题翻译：用于鉴定基因组序列变异和检测错配碱基对（例如单核苷酸多态性）的基于离子的感测
公开(公告)号：US20080197025A1
公开(公告)日：2008-08-21
申请号：US11090944
申请日：2005-03-25
申请人： Yanxiu Zhou , Bin Yu , Kalle Levon
发明人： Yanxiu Zhou , Bin Yu , Kalle Levon
IPC分类号： G01N27/406
CPC分类号： C12Q1/6825 , C12Q2600/156 , G01N27/333 , C12Q2565/518
摘要： Ionic interactions are monitored to detect hybridization. The measurement may be done measuring the potential change in the solution with the ion sensitive electrode (which may be the conducting polymer (e.g., polyaniline) itself), without applying any external energy during the binding. The double helix formation during the complimentary hybridization makes this electrode act as an ion selective electrode—the nucleotide hydrogen bonding is specific and thus monitoring the ionic phosphate group addition becomes selective. Polyaniline on the surface of nylon film forms a positively charged polymer film. Thiol linkage can be utilized for polyaniline modification and thiol-modified single strand oligonucleotide chains can be added to polyaniline. The sensitivity is because the double helix formation during the complimentary hybridization makes this electrode act as an ion selective electrode as the nucleotide hydrogen bonding is specific and thus monitoring the ionic phosphate group addition becomes selective.
摘要翻译：监测离子相互作用以检测杂交。测量可以通过离子敏感电极（其可以是导电聚合物（例如聚苯胺）本身））测量溶液中的潜在变化，而在结合期间不施加任何外部能量。在互补杂交期间的双螺旋形成使得该电极用作离子选择性电极 - 核苷酸氢键是特异性的，因此监测离子性磷酸酯基添加成为选择性的。尼龙膜表面的聚苯胺形成带正电的聚合物膜。硫醇连接可用于聚苯胺改性，硫醇改性的单链寡核苷酸链可以加入到聚苯胺中。敏感性是因为互补杂交期间的双螺旋形成使得该电极作为离子选择性电极，因为核苷酸氢键是特异性的，因此监测离子性磷酸酯基添加成为选择性的。

10. 发明申请

US20090087549A1 SELECTIVE COATING OF FUEL CELL ELECTROCATALYST 审中-公开
标题翻译：燃料电池的选择性涂层
公开(公告)号：US20090087549A1
公开(公告)日：2009-04-02
申请号：US11862425
申请日：2007-09-27
申请人： Yanxiu Zhou , Chowdary Ramesh Koripella , Kajal Parekh
发明人： Yanxiu Zhou , Chowdary Ramesh Koripella , Kajal Parekh
IPC分类号： B05D1/18 , B05D3/00
CPC分类号： H01M4/92 , H01M4/8817 , H01M4/8828 , H01M4/8892 , H01M4/926 , H01M8/1009 , H01M8/1011 , Y02E60/523
摘要： A method is provided for selectively coating a catalyst layer (158) on an electrode of a fuel cell. A porous conductive material (154) comprising gold is formed overlying a portion of a dielectric material (114) to form the electrode. The porous conductive material (154) and the dielectric material (114) are coated with the catalyst layer (158) comprising a carbon supported platinum. The catalyst layer (158) is washed with a solvent to substantially remove the catalyst layer (158) from the dielectric material (114). Optionally, an ionomer component is diffused into the catalyst layer (158) remaining on the porous conductive material (154). The catalyst coated (158) circular channels (156) are then filled with an electrolyte layer (162).
摘要翻译：提供了一种用于在燃料电池的电极上选择性地涂覆催化剂层（158）的方法。包含金的多孔导电材料（154）形成在电介质材料（114）的一部分上方以形成电极。多孔导电材料（154）和介电材料（114）涂覆有包含碳负载的铂的催化剂层（158）。催化剂层（158）用溶剂洗涤以从电介质材料（114）基本上除去催化剂层（158）。任选地，离聚物组分扩散到保留在多孔导电材料（154）上的催化剂层（158）中。催化剂涂覆的（158）圆形通道（156）然后用电解质层（162）填充。

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