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1. 发明申请

US20140205993A1 RECOMBINANT AVIAN INFLUENZA VACCINE AND USES THEREOF 审中-公开
标题翻译：重组AVIAN流感疫苗及其用途
公开(公告)号：US20140205993A1
公开(公告)日：2014-07-24
申请号：US13744806
申请日：2013-01-18
申请人： Xuan Guo , Michel Bublot , Joyce Anita Pritchard , Lynn Dickey
发明人： Xuan Guo , Michel Bublot , Joyce Anita Pritchard , Lynn Dickey
IPC分类号： C07K14/11
CPC分类号： C07K14/005 , A61K39/12 , A61K2039/542 , A61K2039/545 , A61K2039/55566 , C07K2319/02 , C12N2710/24043 , C12N2760/16134 , C12N2760/16151 , C12N2800/22
摘要： The present invention encompasses influenza vaccines, in particular avian influenza vaccines. The vaccine may be a subunit vaccine based on the hemagglutinin of influenza. The hemagglutinin may be expressed in plants including duckweed. The invention also encompasses recombinant vectors encoding and expressing influenza antigens, epitopes or immunogens which can be used to protect animals against influenza. It encompasses also a vaccination regimen compatible with the DIVA strategy, including a prime-boost scheme using vector and subunit vaccines.
摘要翻译：本发明包括流感疫苗，特别是禽流感疫苗。疫苗可以是基于流感血凝素的亚单位疫苗。血凝素可以在植物中表达，包括浮萍。本发明还包括编码和表达可用于保护动物免受流感的流感抗原，表位或免疫原的重组载体。它还包括与DIVA策略相一致的疫苗接种方案，包括使用载体和亚单位疫苗的初次加强方案。

2. 发明申请

US20140205619A1 RECOMBINANT AVIAN INFLUENZA VACCINE AND USES THEREOF 审中-公开
公开(公告)号：US20140205619A1
公开(公告)日：2014-07-24
申请号：US14153512
申请日：2014-01-13
申请人： Xuan Guo , Michel Bublot , Joyce Anita Pritchard , Lynn Dickey
发明人： Xuan Guo , Michel Bublot , Joyce Anita Pritchard , Lynn Dickey
IPC分类号： C07K14/005 , G01N33/569 , A61K39/145
CPC分类号： C07K14/005 , A61K39/12 , A61K39/145 , A61K2039/552 , A61K2039/55566 , C12N2760/16134 , C12N2799/026 , G01N33/56983 , G01N2333/11
摘要： The present invention encompasses influenza vaccines, in particular avian influenza vaccines. The vaccine may be a subunit vaccine based on the hemagglutinin of influenza. The hemagglutinin may be expressed in plants including duckweed. The invention also encompasses recombinant vectors encoding and expressing influenza antigens, epitopes or immunogens which can be used to protect animals against influenza. It encompasses also a vaccination regimen compatible with the DIVA strategy, including a prime-boost scheme using vector and subunit vaccines.

3. 发明申请

US20060195946A1 Expression of biologically active polypeptides in duckweed 失效
公开(公告)号：US20060195946A1
公开(公告)日：2006-08-31
申请号：US10675011
申请日：2003-09-30
申请人： Lynn Dickey , John Gasdaska , Kevin Cox
发明人： Lynn Dickey , John Gasdaska , Kevin Cox
IPC分类号： A01H1/00 , A01H5/00 , C12N15/82 , C07K16/18
CPC分类号： C12N15/8216 , C07K14/56 , C07K2317/55 , C12N15/8257
摘要： Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.

4. 发明申请

US20050060776A1 Expression of biologically active polypeptides in duckweed 审中-公开
标题翻译：生物活性多肽在浮萍中的表达
公开(公告)号：US20050060776A1
公开(公告)日：2005-03-17
申请号：US10873846
申请日：2004-06-22
申请人： Anne-Marie Stomp , Lynn Dickey , John Gasdaska
发明人： Anne-Marie Stomp , Lynn Dickey , John Gasdaska
IPC分类号： C12N15/09 , C07K14/56 , C12N5/10 , C12N9/00 , C12N15/82 , C12P21/02 , C12P21/08 , A01H1/00
CPC分类号： C07K14/56 , C12N15/8216 , C12N15/8257
摘要： Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.
摘要翻译：提供了用于从遗传工程化的浮萍中表达和分泌生物活性多肽的方法，核酸序列和转化的浮萍植物或浮萍结核菌培养物。通过修饰编码多肽的表达盒的核苷酸序列来改善浮萍中重组多肽的表达，从而改善浮萍中的表达。通过将生物活性多肽与指导多肽分泌到培养基中的信号肽连接来改善从浮萍中回收生物活性多肽。

5. 发明申请

US20110250177A1 ALPHA INTERFERON VARIANTS 失效
标题翻译：阿尔法干扰素
公开(公告)号：US20110250177A1
公开(公告)日：2011-10-13
申请号：US13105571
申请日：2011-05-11
申请人： Lynn Dickey , John Gasdaska , Kevin Cox
发明人： Lynn Dickey , John Gasdaska , Kevin Cox
IPC分类号： A61K38/21 , C07K14/435 , A61P29/00 , C07K14/39 , A61P31/12 , A61P35/00 , C07K14/00 , C07K14/415
CPC分类号： C07K14/56 , C07K2317/55 , C12N15/8216 , C12N15/8257
摘要： The present invention provides biologically active variants of human α-2b-interferon. The variants contain carboxy terminus truncations when compared with the amino acid sequence of full-length human α-2b-interferon. It is the novel finding of the present invention that these truncated variants have the biological activity of full-length human α-2b-interferon. The invention encompasses these biologically active variant α-interferons, as well as polynucleotides encoding these interferons. Expression cassettes comprising these polynucleotides and host cells comprising the expression cassettes are also provided. The invention also provides compositions comprising variant α-interferon polypeptides and a pharmaceutically acceptable carrier.
摘要翻译：本发明提供人α-2b-干扰素的生物活性变体。当与全长人α-2b-干扰素的氨基酸序列相比时，变体含有羧基末端截短。本发明的新发现是这些截短的变体具有全长人α-2b-干扰素的生物学活性。本发明包括这些生物活性变体α-干扰素，以及编码这些干扰素的多核苷酸。还提供了包含这些多核苷酸的表达盒和包含表达盒的宿主细胞。本发明还提供包含变体α-干扰素多肽和药学上可接受的载体的组合物。

6. 发明授权

US07709699B2 Use of duckweed in high throughput screening 失效
标题翻译：在高通量筛选中使用浮萍
公开(公告)号：US07709699B2
公开(公告)日：2010-05-04
申请号：US10158243
申请日：2002-05-30
申请人： Anne-Marie Stomp , Lynn Dickey , Billy Houghteling , Nirmala Rajbhandari
发明人： Anne-Marie Stomp , Lynn Dickey , Billy Houghteling , Nirmala Rajbhandari
IPC分类号： A01H1/00 , A01H9/00 , A01H11/00 , C12N5/04 , C12N5/10 , B01L3/00
CPC分类号： G01N33/5097 , C12N15/8257 , C12Q1/6897
摘要： Methods for high-throughput screening in duckweed are disclosed. In one aspect, these methods are used to identify nucleotide sequences encoding polypeptides of interest. In another aspect, these methods are used to identify nucleotide sequences that modulate the expression of a target nucleotide sequence. The methods combine the predictive benefits of screening in whole plants with the speed and efficiency of a high throughput system.
摘要翻译：披露了浮萍高通量筛选方法。在一个方面，这些方法用于鉴定编码目的多肽的核苷酸序列。另一方面，这些方法用于鉴定调节靶核苷酸序列表达的核苷酸序列。该方法将整个植物筛选的预测益处与高通量系统的速度和效率相结合。

7. 发明申请

US20080060092A1 COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS 失效
标题翻译： N-GLYCANS在植物中的人类化和优化的组合物和方法
公开(公告)号：US20080060092A1
公开(公告)日：2008-03-06
申请号：US11624158
申请日：2007-01-17
申请人： Lynn Dickey , Kevin Cox , Charles Peele , Ming-Bo Wang
发明人： Lynn Dickey , Kevin Cox , Charles Peele , Ming-Bo Wang
IPC分类号： A01H5/00 , C07H21/04 , C07K14/00 , C07K16/18 , C12N15/00 , C12N15/82 , C12N5/04
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of α1,3-fucosyltransferase (FucT) and β1,2-xylosyltransferase (XylT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a “humanized” N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the G0 glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白质N-糖基化模式的方法。所述方法包括将植物中的α1,3-岩藻糖基转移酶（FucT）和β1,2-木糖基转移酶（XylT）的表达抑制的重组构建体引入植物中。这些构建体用于抑制或抑制这两种酶及其同种型的表达有利地提供具有“人源化”N-糖基化模式的内源和异源蛋白质的生产，而不影响植物的生长和发育。提供了具有这种蛋白质N-糖基化模式的稳定转化的高等植物。还提供了具有基本上均匀的糖基化曲线并且对于G0糖型基本均匀的糖蛋白组合物，包括单克隆抗体组合物。

8. 发明申请

US20050262592A1 Expression of plasminogen and microplasminogen in duck weed 失效
标题翻译：在鸭杂草中表达纤溶酶原和微量淀粉样蛋白
公开(公告)号：US20050262592A1
公开(公告)日：2005-11-24
申请号：US11056621
申请日：2005-02-11
申请人： David Spencer , Lynn Dickey , John Gasdaska , Xiaowei Wang , Kevin Cox , Charles Peele
发明人： David Spencer , Lynn Dickey , John Gasdaska , Xiaowei Wang , Kevin Cox , Charles Peele
IPC分类号： A01H1/00 , C12N9/68 , C12N15/82
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

9. 发明授权

US08022270B2 Expression of biologically active polypeptides in duckweed 失效
标题翻译：生物活性多肽在浮萍中的表达
公开(公告)号：US08022270B2
公开(公告)日：2011-09-20
申请号：US10675011
申请日：2003-09-30
申请人： Lynn Dickey , John Gasdaska , Kevin Cox
发明人： Lynn Dickey , John Gasdaska , Kevin Cox
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00
CPC分类号： C12N15/8216 , C07K14/56 , C07K2317/55 , C12N15/8257
摘要： Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.
摘要翻译：提供了用于从遗传工程化的浮萍中表达和分泌生物活性多肽的方法，核酸序列和转化的浮萍植物或浮萍结核菌培养物。通过修饰编码多肽的表达盒的核苷酸序列来改善浮萍中重组多肽的表达，从而改善浮萍中的表达。通过将生物活性多肽与指导多肽分泌到培养基中的信号肽连接来改善从浮萍中回收生物活性多肽。

10. 发明授权

US07959910B2 C-terminally truncated interferon alpha variants 失效
标题翻译： C-末端截短的干扰素α变体
公开(公告)号：US07959910B2
公开(公告)日：2011-06-14
申请号：US10574046
申请日：2004-04-16
申请人： Lynn Dickey , John Gasdaska , Kevin Cox
发明人： Lynn Dickey , John Gasdaska , Kevin Cox
IPC分类号： A61K38/21 , C07K14/56 , C12P21/02 , C07H21/04 , C12N5/14
CPC分类号： C07K14/56 , C07K2317/55 , C12N15/8216 , C12N15/8257
摘要： The present invention provides biologically active variants of human α-2b-interferon. The variants contain carboxy terminus truncations when compared with the amino acid sequence of full-length human α-2b-interferon. It is the novel finding of the present invention that these truncated variants have the biological activity of full-length human α-2b-interferon. The invention encompasses these biologically active variant α-interferons, as well as polynucleotides encoding these interferons. Expression cassettes comprising these polynucleotides and host cells comprising the expression cassettes are also provided. The invention also provides compositions comprising variant α-interferon polypeptides and a pharmaceutically acceptable carrier.
摘要翻译：本发明提供人α-2b-干扰素的生物活性变体。当与全长人α-2b-干扰素的氨基酸序列相比时，变体含有羧基末端截短。本发明的新发现是这些截短的变体具有全长人α-2b-干扰素的生物学活性。本发明包括这些生物活性变体α-干扰素，以及编码这些干扰素的多核苷酸。还提供了包含这些多核苷酸的表达盒和包含表达盒的宿主细胞。本发明还提供包含变体α-干扰素多肽和药学上可接受的载体的组合物。

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