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1. 发明申请

US20110237460A1 MICROARRAY PACKAGE DEVICE AND METHOD OF MANUFACTURING THE SAME 有权
标题翻译：微型包装装置及其制造方法
公开(公告)号：US20110237460A1
公开(公告)日：2011-09-29
申请号：US12938485
申请日：2010-11-03
申请人： Woochang LEE , Jeo-young SHIM , Myo-yong LEE , Kak NAMKOONG , Won-seok CHUNG
发明人： Woochang LEE , Jeo-young SHIM , Myo-yong LEE , Kak NAMKOONG , Won-seok CHUNG
IPC分类号： C40B40/00 , C40B50/00
CPC分类号： C40B60/12 , B01L3/508 , B01L2200/025 , B01L2300/0819
摘要： A microarray package device and a method of manufacturing the same. An effective microarray analyzing reaction is performed by using the microarray package device that provides structural stability and reliable experimental results.
摘要翻译：微阵列包装装置及其制造方法。通过使用提供结构稳定性和可靠的实验结果的微阵列包装装置进行有效的微阵列分析反应。

2. 发明申请

US20110190147A1 MICROARRAY REACTION DEVICE AND METHOD OF USING THE SAME 失效
标题翻译：微波反应装置及其使用方法
公开(公告)号：US20110190147A1
公开(公告)日：2011-08-04
申请号：US13007750
申请日：2011-01-17
申请人： Won-seok CHUNG , Myo-yong LEE , Kak NAMKOONG , Woochang LEE
发明人： Won-seok CHUNG , Myo-yong LEE , Kak NAMKOONG , Woochang LEE
IPC分类号： C40B30/00 , C40B60/12 , C40B40/06 , C40B40/10
CPC分类号： C40B30/00 , C40B40/06 , C40B40/10 , C40B60/12
摘要： A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber.
摘要翻译：微阵列反应装置包括流体容器，反应室，与流体容器连接的第一通道，与反应室连接的第二通道和阀。阀包括第一和第二支撑单元，分别包括延伸穿过其第一和第二表面的第一和第二穿透开口单元。第一和第二穿透开口单元分别连接到第一和第二通道的第二端。第二支撑单元包括延伸穿过其第二表面的第三穿透开口单元。第一和第二表面彼此接触，使得第一支撑单元和第二支撑单元彼此可滑动地布置。微阵列反应装置还包括与第三穿透开口单元连接的储存室，以及连接到储存室并向储存室提供压力的泵。

3. 发明申请

US20080306249A1 METHOD OF SELECTIVELY REMOVING PROTEIN FROM BIOLOGICAL SAMPLES USING CHEMICALS 审中-公开
标题翻译：使用化学品从生物样品中选择性去除蛋白质的方法
公开(公告)号：US20080306249A1
公开(公告)日：2008-12-11
申请号：US12107498
申请日：2008-04-22
申请人： Myo-yong LEE , Ki-woong HAN
发明人： Myo-yong LEE , Ki-woong HAN
IPC分类号： C07K1/00
CPC分类号： C07K1/36 , C07K1/20 , C12Q1/6806 , C12Q2527/125
摘要： Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom or a substituted or unsubstituted C1-C6 alkyl group, a is an integer of 1 to 6 and b is 0 or 1, wherein b is 0 when a is not 1; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.
摘要翻译：提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中R1，R2中的至少两个和R3取代基是取代或未取代的C1-C6烷基，另一个取代基是氢原子或取代或未取代的C1-C6烷基，a是1〜6的整数，b是0或1，其中b是当a不为1时为0; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。

4. 发明申请

US20080070250A1 METHOD OF REMOVING AIR BUBBLES FROM HYBRIDIZATION SOLUTION OF MICROARRAY-COVERSLIP ASSEMBLY AND MICROARRAY KIT FOR THE SAME 失效
标题翻译：从微胶囊装配混合溶液中除去空气泡沫的方法及其相同的微量试剂盒
公开(公告)号：US20080070250A1
公开(公告)日：2008-03-20
申请号：US11736033
申请日：2007-04-17
申请人： Myo-yong LEE
发明人： Myo-yong LEE
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L3/50857 , B01L2200/0647 , B01L2200/0684 , B01L2200/14 , B01L2300/0819 , B01L2300/0822 , B01L2400/043 , C12Q1/6837
摘要： A method of removing air bubbles from a hybridization solution in a microarray-coverslip assembly c including injecting a solution of magnetic particles between the coverslip and microarray, applying a magnetic field to the microarray-coverslip assembly, moving the magnetic particles and removing air bubbles in the hybridization solution from a hybridization area of the microarray with the magnetic particles, and a microarray kit for the same.
摘要翻译：一种在微阵列盖玻片组件c中从杂交溶液中除去气泡的方法，包括在盖玻片和微阵列之间注入磁性颗粒溶液，向微阵列 - 盖玻片组件施加磁场，移动磁性颗粒并除去气泡来自微阵列与磁性颗粒的杂交区域的杂交溶液，以及用于其的微阵列试剂盒。

5. 发明申请

US20100190651A1 METHOD OF ANALYZING PROBE NUCLEIC ACID, MICROARRAY AND KIT FOR THE SAME 有权
标题翻译：分析探针核酸，微阵列和相同试剂盒的方法
公开(公告)号：US20100190651A1
公开(公告)日：2010-07-29
申请号：US12684345
申请日：2010-01-08
申请人： Kyung-hee PARK , Myo-yong LEE , Kyu-sang LEE , Jong-suk CHUNG
发明人： Kyung-hee PARK , Myo-yong LEE , Kyu-sang LEE , Jong-suk CHUNG
IPC分类号： C40B30/00
CPC分类号： C12Q1/6837 , C12Q2545/101
摘要： Provided are a method of analyzing a sequence of a first probe nucleic acid using a substrate on which a second probe nucleic acid is immobilized, and a microarray and a kit for the same.
摘要翻译：提供了使用其上固定有第二探针核酸的底物和微阵列及其用于分析第一探针核酸的试剂盒的方法来分析第一探针核酸的序列。

6. 发明申请

US20080199930A1 METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER 审中-公开
标题翻译：使用微珠和激光快速破坏细胞或病毒的方法和装置
公开(公告)号：US20080199930A1
公开(公告)日：2008-08-21
申请号：US12031947
申请日：2008-02-15
申请人： Jeong-gun LEE , Young-nam KWON , Young-a KIM , Myo-yong LEE , Shin-i YOO , Yeon-ja CHO , Kwang-ho CHEONG , Chang-eun YOO , Seung-yeon YANG
发明人： Jeong-gun LEE , Young-nam KWON , Young-a KIM , Myo-yong LEE , Shin-i YOO , Yeon-ja CHO , Kwang-ho CHEONG , Chang-eun YOO , Seung-yeon YANG
IPC分类号： C12N13/00 , C12M1/00
CPC分类号： C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
摘要： A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
摘要翻译：提供了使用珠和激光来快速破坏细胞或病毒的方法和装置。根据使用珠粒和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，可以使用激光二极管使装置小型化，DNA纯化步骤可以在细胞破碎后直接进行或病毒，并且含有DNA的溶液可以转移到随后的步骤中，在用电磁体除去后续反应的抑制剂附着的细胞碎片和珠粒之后。另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决裂解芯片可应用于LOC。

7. 发明申请

US20130078658A1 METHOD OF QUANTIFYING RECOVERY RATE OF EXOSOME 审中-公开
标题翻译：定量恢复率的方法
公开(公告)号：US20130078658A1
公开(公告)日：2013-03-28
申请号：US13491258
申请日：2012-06-07
申请人： Dong-hyun PARK , Hyun-jo KANG , Ko-bong CHOI , Myo-yong LEE
发明人： Dong-hyun PARK , Hyun-jo KANG , Ko-bong CHOI , Myo-yong LEE
IPC分类号： G01N21/76 , G01N21/64 , C12N9/96 , C12P21/00
CPC分类号： C07K14/705 , C07K14/70596 , C07K2319/60
摘要： A recombinant exosome comprising a fusion protein of a membrane protein and light-emitting protein, and a method of determining an exosome recovery rate by using the recombinant exosome are provided. Use of the method ensures accurate quantification of exosomes in a sample, and thus, improves the efficiency of an exosome-based diagnosis.
摘要翻译：提供了包含膜蛋白和发光蛋白的融合蛋白的重组外来体，以及通过使用重组外来体确定外来体回收率的方法。使用该方法确保样品中外来体的准确定量，从而提高基于外来体的诊断的效率。

8. 发明申请

US20090215653A1 DEVICE FOR HYBRIDIZATION CHAMBERS PREVENTING AIR BUBBLES AND HYBRIDIZATION DEVICE COMPRISING THE SAME DEVICE 审中-公开
标题翻译：用于防止空气泡沫的混合室的装置和包含相同装置的混合装置
公开(公告)号：US20090215653A1
公开(公告)日：2009-08-27
申请号：US11850917
申请日：2007-09-06
申请人： Myo-yong LEE
发明人： Myo-yong LEE
IPC分类号： C40B60/00
CPC分类号： B01L3/508 , B01L9/527 , B01L2200/0684 , G01N2035/00306
摘要： A device for a hybridization chamber includes a support including an engagement receiving member which receives a microarray, the engagement receiving member engages with the microarray, a hybridization chamber frame which forms the hybridization chamber when in contact with the microarray, a sealing member disposed on the hybridization frame, the sealing member defines a region of the hybridization chamber and a cover coupled with the support and the hybridization chamber frame, wherein one end of the cover is coupled with the support using a hinge, and an opposite end of the cover includes a compression coupling means.
摘要翻译：用于杂交室的装置包括支撑件，其包括接收微阵列的接合接收构件，接合接收构件与微阵列接合，当与微阵列接触时形成杂交室的杂交室框架，设置在微阵列上的密封构件杂交框架，密封构件限定杂交室的区域和与支撑件和杂交室框架联接的盖，其中盖的一端使用铰链与支撑件联接，并且盖的相对端包括压缩联接装置。

9. 发明申请

US20090163382A1 PRIMER SET FOR AMPLIFYING TARGET SEQUENCE(S) OF ANTIBIOTIC-RESISTANT BACTERIAL SPECIES, PROBE OR PROBE SET SPECIFICALLY HYBRIDIZING WITH TARGET SEQUENCE(S) OF ANTIBIOTIC-RESISTANT BACTERIAL SPECIES, METHOD OF DETECTING ANTIBIOTIC-RESISTANT BACTERIAL SPECIES USING THE PROBE OR PROBE SET, AND KIT FOR DETECTING ANTIBIOTIC-RESISTANT BACTERIAL SPECIES 审中-公开
标题翻译：用于放大抗生素细菌物种的目标序列的初步设置，特异性地与抗生素耐性细菌物种的目标序列混合的探针或探针，使用探针或探针组检测抗生素耐性细菌物种的方法，和用于检测抗生素细菌物种的工具包
公开(公告)号：US20090163382A1
公开(公告)日：2009-06-25
申请号：US11863984
申请日：2007-09-28
申请人： Ji-Young OH , Yeon-su LEE , Sang-hyun PAEK , Byung-chul KIM , Sook-young KIM , Kyung-hee PARK , Jung-nam LEE , Jong-suk CHUNG , Ah-gi KIM , Myo-yong LEE , Tae-jin AHN
发明人： Ji-Young OH , Yeon-su LEE , Sang-hyun PAEK , Byung-chul KIM , Sook-young KIM , Kyung-hee PARK , Jung-nam LEE , Jong-suk CHUNG , Ah-gi KIM , Myo-yong LEE , Tae-jin AHN
IPC分类号： C40B40/08 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Provided are a primer set for amplifying target sequence(s) of antibiotic-resistant bacterial species, a probe or probe set specifically hybridizing with target sequence(s) of antibiotic-resistant bacterial species, a microarray immobilized with the probe or probe set, a kit comprising the primer set and a method of detecting at least one antibiotic-resistant bacterial species using the probe or probe set.
摘要翻译：提供了用于扩增抗生素抗性细菌种类的靶序列的引物组，与抗生素抗性细菌种类的靶序列特异性杂交的探针或探针组，固定有探针或探针组的微阵列包括引物组的试剂盒和使用探针或探针组检测至少一种抗生素抗性细菌物种的方法。

10. 发明申请

US20090131649A1 METHOD OF SEPARATING SMALL RNA MOLECULES USING KOSMOTROPIC SALT 有权
标题翻译：使用KOSMOTROPIC盐分离小RNA分子的方法
公开(公告)号：US20090131649A1
公开(公告)日：2009-05-21
申请号：US12110373
申请日：2008-04-28
申请人： Myo-yong LEE , Nam Huh , Joon-ho Kim
发明人： Myo-yong LEE , Nam Huh , Joon-ho Kim
IPC分类号： C07H1/00
CPC分类号： C12N15/1006
摘要： Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations.
摘要翻译：公开了一种使用不同浓度的可变性盐分离从固体支持物上的较大RNA分离200个核苷酸或更少的小RNA的方法。

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