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1. 发明授权

US5369003A Process for the specific production of ribonucleic acids 失效
标题翻译：具体生产核糖核酸的方法
公开(公告)号：US5369003A
公开(公告)日：1994-11-29
申请号：US951983
申请日：1992-09-28
申请人： Udo Reischl , Rudiger Rueger , Christoph Kessler , Rudolf Seibl
发明人： Udo Reischl , Rudiger Rueger , Christoph Kessler , Rudolf Seibl
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6865
摘要： Process for the production of ribonucleic acids or for the detection of template nucleic acids based on a transcription technique using two oligonucleotides which can hybridize to adjacent regions on the template nucleic acid without ligation of these oligonucleotides.
摘要翻译：用于生产核糖核酸或用于基于转录技术检测模板核酸的方法，其使用两种寡核苷酸，其可以与模板核酸上的相邻区域杂交而不连接这些寡核苷酸。

2. 发明授权

US5702888A Process for the detection of nucleic acids 失效
标题翻译：检测核酸的方法
公开(公告)号：US5702888A
公开(公告)日：1997-12-30
申请号：US371320
申请日：1995-01-11
申请人： Hans-Joachim Holtke , Rudolf Seibl , Gudrun Schmitz , Hans Robert Scholer , Christoph Kessler , Ralf Mattes
发明人： Hans-Joachim Holtke , Rudolf Seibl , Gudrun Schmitz , Hans Robert Scholer , Christoph Kessler , Ralf Mattes
IPC分类号： G01N33/53 , C07H21/00 , C12Q1/68 , G01N33/567 , C07H21/04
CPC分类号： C12Q1/6816 , C07H21/00 , C12Q1/6804 , C12Q1/686
摘要： For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound vie a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.
摘要翻译：为了通过与互补核酸探针杂交来检测确定序列的核酸，所述互补核酸探针含有通过化学键合键合至少一个半抗原作为标记，使用作为半抗原的类固醇，所述类固醇结合至少4个原子长度的桥至不参与氢桥形成的核酸探针的至少一个位置，并通过反过来标记的抗半抗原抗体检测杂交的探针。

3. 发明授权

US5753433A Method for the sensitive detection of nucleic acids 失效
标题翻译：敏感检测核酸的方法
公开(公告)号：US5753433A
公开(公告)日：1998-05-19
申请号：US307414
申请日：1994-09-19
申请人： Christoph Kessler , Rudiger Ruger , Rudolf Seibl , Cornelia Kruse-Muller , Sibylle Berner
发明人： Christoph Kessler , Rudiger Ruger , Rudolf Seibl , Cornelia Kruse-Muller , Sibylle Berner
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/6855
摘要： Method for the specific detection of nucleic acids in a sample by reaction of the sample with one or several labelled nucleotide triphosphates and one or several enzymes which catalyse the production of a labelled nucleic acid B which contains this nucleotide, non-thermally denaturation, reacting the sample with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group, if desired, contacting the nucleic acid hybrid D formed with a solid phase which recognizes and binds the immobilizable group, removing the liquid from the solid phase and determining the label on the solid phase as a measure for the presence of the nucleic acid.
摘要翻译：用于通过样品与一种或几种标记的三磷酸标记的核苷酸和一种或多种催化产生含有该核苷酸的非热变性的标记核酸B的产生的酶来反应样品中的核酸的方法，用与核酸B充分互补的核酸探针C的样品，如果需要，其含有至少一个可固定基团，如果需要，将形成的核酸混合物D与识别并结合可固化基团的固相接触，从固相和确定固相上的标记作为核酸存在的量度。

4. 发明授权

US5635350A Reagents and kits for determining polymerase activity 失效
标题翻译：用于测定聚合酶活性的试剂和试剂盒
公开(公告)号：US5635350A
公开(公告)日：1997-06-03
申请号：US382891
申请日：1995-02-03
申请人： Josef Eberle , Rudolf Seibl , Christoph Kessler , Bernhard Konig
发明人： Josef Eberle , Rudolf Seibl , Christoph Kessler , Bernhard Konig
IPC分类号： C12Q1/48 , C12Q1/68 , C12Q1/70 , G01N33/573
CPC分类号： G01N33/573 , C12Q1/48 , C12Q1/703 , Y10S435/81
摘要： Method of determining polymerase activity by incubating the polymerase with a template nucleic acid, one detectable mononucleoside triphosphate and one immobilizable nucleoside triphosphate, binding the immobilizable nucleotides to a solid phase and detecting the bound delectable nucleotides and tests based thereon.
摘要翻译：通过用模板核酸，一种可检测的单核苷三磷酸和一种可固定核苷三磷酸孵育聚合酶来测定聚合酶活性的方法，将可固定的核苷酸与固相结合，并基于此检测结合的可选择的核苷酸和测试。

5. 发明授权

US5344757A Process for the detection of nucleic acids 失效
标题翻译：检测核酸的方法
公开(公告)号：US5344757A
公开(公告)日：1994-09-06
申请号：US415307
申请日：1990-01-09
申请人： Hans-Joachim Holtke , Rudolf Seibl , Gudrun Schmitz , Hans R. Scholer , Christoph Kessler , Ralf Mattes
发明人： Hans-Joachim Holtke , Rudolf Seibl , Gudrun Schmitz , Hans R. Scholer , Christoph Kessler , Ralf Mattes
IPC分类号： G01N33/53 , C07H21/00 , C12Q1/68 , G01N33/567
CPC分类号： C12Q1/6816 , C07H21/00 , C12Q1/6804 , C12Q1/686
摘要： For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound via a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.
摘要翻译： PCT No.PCT / EP89 / 00026 Sec。 371 1990年1月9日第 102（e）1990年1月9日PCT PCT 1月12日PCT PCT。出版物WO89 / 06698 日期为1989年7月27日。为了通过与互补核酸探针杂交来检测确定序列的核酸，所述互补核酸探针含有通过化学键合结合至少一个半抗原作为标记，使用作为半抗原的类固醇，其通过至少4个原子长度的桥与至少一个不参与氢桥形成的核酸探针的位置，并通过反过来标记的抗半抗原抗体检测杂交的探针。

6. 发明授权

US5413906A Method of determining polymerase activity 失效
标题翻译：确定聚合酶活性的方法
公开(公告)号：US5413906A
公开(公告)日：1995-05-09
申请号：US974595
申请日：1993-03-19
申请人： Josef Eberle , Rudolf Seibl , Christoph Kessler , Bernhard Konig
发明人： Josef Eberle , Rudolf Seibl , Christoph Kessler , Bernhard Konig
IPC分类号： C12Q1/48 , C12Q1/68 , C12Q1/70 , G01N33/573 , C12P19/34
CPC分类号： G01N33/573 , C12Q1/48 , C12Q1/703 , Y10S435/81
摘要： Method of determining polymerase activity by incubating the polymerase with a template nucleic acid, one detectable mononucleoside triphosphate and one immobilizable nucleoside triphosphate, binding the immobilizable nucleotides to a solid phase and detecting the bound detectable nucleotides and tests based thereon.
摘要翻译： PCT No.PCT / EP91 / 01016 Sec。 371日期1993年3月19日 102（e）1993年3月19日PCT PCT 1991年5月31日PCT。出版物WO92 / 04467 1992年3月19日。通过用模板核酸，一个可检测的单核苷三磷酸和一个可固定的核苷三磷酸孵育聚合酶来测定聚合酶活性的方法，将可固定的核苷酸与固相结合并检测结合的可检测的核苷酸并基于其测试。

7. 发明授权

US5354657A Process for the highly specific detection of nucleic acids in solid 失效
标题翻译：在溶液中高度特异性检测核酸的方法
公开(公告)号：US5354657A
公开(公告)日：1994-10-11
申请号：US993820
申请日：1992-12-18
申请人： Hans J. Holtke , Rudolf Seibl , Christoph Kessler , Ralf Mattes , Hermann Graf
发明人： Hans J. Holtke , Rudolf Seibl , Christoph Kessler , Ralf Mattes , Hermann Graf
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/04 , C12N15/00
CPC分类号： C07H21/00 , C12Q1/6804
摘要： The present invention provides a process for the detection of nucleic acids of definite sequence by hybridisation with two single-stranded nucleic acid probes present in the same solution phase complementary to different regions of the nucleic acid to be detected, one nucleic acid probe serving as detector probe and containing as labelling at least one hapten bound via a chemical linkage and the other nucleic acid probe serving as capturing probe and being bound to a solid matrix, wherein, as hapten, a steroid is used which on at least one position of the detector probe, which does not participate in the hydrogen bridge formation with the nucleic acid to be detected, is covalently bound via a bridge of at least four atoms, the nucleic acid to be detected, present in solution, is incubated with the detector probe and the capturing probe in any desired sequence, whereby the binding of the capturing probe on the matrix with the nucleic acid to be detected is brought about before, during or after the incubation, the remaining solution is separated off from the matrix-bound complex of the capturing probe, nucleic acid to be detected and detector probe and the complex detected via an anti-hapten antibody which is labelled.
摘要翻译：本发明提供了通过与存在于与要检测的核酸的不同区域互补的相同溶液相中的两个单链核酸探针杂交来检测确定序列的核酸的方法，一个用作检测器的核酸探针探针并包含作为标记至少一个通过化学连接结合的半抗原，并且另一个核酸探针用作捕获探针并结合到固体基质上，其中作为半抗原，使用类固醇，其在检测器的至少一个位置上不参与与要检测的核酸的氢桥形成的探针通过至少四个原子的桥共价结合，将存在于溶液中的要检测的核酸与检测器探针和以任何期望的顺序捕获探针，由此捕获探针在基质上与待检测的核酸的结合在 r后，将剩余的溶液与捕获探针的基质结合复合物，待检测的核酸和检测器探针分离，并通过经标记的抗半抗原抗体检测复合物。

8. 发明授权

US6001610A Method for the particularly sensitive detection of nucleic acids 失效
标题翻译：核酸特异性敏感检测方法
公开(公告)号：US6001610A
公开(公告)日：1999-12-14
申请号：US20538
申请日：1998-02-09
申请人： Rudolf Seibl , Viola Rosemeyer
发明人： Rudolf Seibl , Viola Rosemeyer
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6853 , C12Q1/682 , C12Q1/6823 , C12Q1/6844 , Y10S435/875
摘要： A method of detecting a target nucleic acid A is disclosed, comprising hybridizing the target nucleic acid A with a probe nucleic acid B which contains a sequence B1 which base pairs with a part of the target nucleic acid A and a sequence B2, cleaving the hybridized probe nucleic acid B to produce a cleavage product B' containing the sequence B2, hybridizing the cleavage product B' with a template nucleic acid C containing a sequence C2 which base pairs with a part of the cleavage product B' and a sequence C1 which does not hybridize with the sequence B1 of the probe nucleic acid B, extending the hybridized cleavage product B' with an extension sequence B3 which is template-specific to a part of the sequence C1, hybridizing a probe D with the extension product, wherein the probe D contains a sequence D1 which base pairs with the extension sequence B3 and a sequence D2, and detecting any of the various products formed throughout the method. Products for performing the method are also disclosed.
摘要翻译：公开了一种检测靶核酸A的方法，包括使靶核酸A与包含与靶核酸A的一部分碱基对的序列B1和序列B2的探针核酸B杂交，所述序列B1切割杂交的探针核酸B以产生含有序列B2的切割产物B'，将裂解产物B'与含有一部分切割产物B'的碱基对的序列C2的模板核酸C和与切割产物B'的一部分的序列C1杂交不与探针核酸B的序列B1杂交，用扩增序列B3延伸杂交的切割产物B'，延伸序列B3是序列C1的一部分的模板特异性，将探针D与延伸产物杂交，其中探针 D包含与扩展序列B3和序列D2碱基对的序列D1，并检测整个方法中形成的各种产物中的任何一种。还公开了用于执行该方法的产品。

9. 发明授权

US5830664A Method for the detection of target nucleic acid 失效
标题翻译：检测靶核酸的方法
公开(公告)号：US5830664A
公开(公告)日：1998-11-03
申请号：US503058
申请日：1995-07-17
申请人： Viola Rosemeyer , Rudolf Seibl
发明人： Viola Rosemeyer , Rudolf Seibl
IPC分类号： G01N33/58 , C07H21/00 , C12N15/09 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6813 , C12Q1/6865
摘要： Method for the sensitive detection of a target nucleic acid by hybridization with a probe nucleic acid. The latter contains a part which can hybridize with the target nucleic acid and a nucleic acid-specific part which does not hybridize with the target nucleic acid. The method further comprises cleavage of the probe nucleic acid, hybridization of a cleavage product of the probe nucleic acid containing the part that does not hybridize with the target nucleic acid with a matrix nucleic acid containing a part that can be hybridized with the cleavage product and a part that cannot be hybridized with the probe nucleic acid. The method also comprises the determination of the hybrid consisting of the cleavage product and the matrix nucleic acid and a reagent kit suitable for this purpose.
摘要翻译：通过与探针核酸杂交来灵敏检测靶核酸的方法。后者含有可与靶核酸杂交的部分和不与靶核酸杂交的核酸特异性部分。该方法还包括探针核酸的切割，将含有不与靶核酸杂交的部分的探针核酸的裂解产物与含有可与切割产物杂交的部分的基质核酸杂交，以及不能与探针核酸杂交的部分。该方法还包括由切割产物和基质核酸组成的杂交物的测定以及适用于该目的的试剂盒。

10. 发明授权

US5989812A Transcription method 失效
标题翻译：转录方法
公开(公告)号：US5989812A
公开(公告)日：1999-11-23
申请号：US561637
申请日：1995-11-22
申请人： Viola Rosemeyer , Rudolf Seibl
发明人： Viola Rosemeyer , Rudolf Seibl
IPC分类号： C12N15/09 , C07H21/04 , C12P19/34 , C12Q1/68 , C07H21/02
CPC分类号： C12P19/34
摘要： An improved method of transcribing nucleic acids wherein the amount of abortive transcripts formed is reduced wherein the improvement comprises an effector sequence in the transcription reaction.
摘要翻译：一种转录核酸的改进方法，其中形成的流产转录物的量减少，其中改良包括转录反应中的效应序列。

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