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1. 发明授权

US08144396B2 Microscope apparatus 有权
标题翻译：显微镜装置
公开(公告)号：US08144396B2
公开(公告)日：2012-03-27
申请号：US12060994
申请日：2008-04-02
申请人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
发明人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
IPC分类号： G02B21/26
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译：在长期观察期间，可以防止图像变得不清楚。本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。

2. 发明授权

US07876500B2 Microscope apparatus and microscope system 有权
标题翻译：显微镜仪器和显微镜系统
公开(公告)号：US07876500B2
公开(公告)日：2011-01-25
申请号：US12059043
申请日：2008-03-31
申请人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
发明人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
IPC分类号： G02B21/00
CPC分类号： G02B21/367
摘要： It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.
摘要翻译：可以检查观察成功或失败以及观察历史，而不等待观察完全完成，从而节省观察所需的时间和精力，并避免丢失观察珍贵样品等的机会。提供一种显微镜装置，包括图像获取单元，用于在改变多个参数的同时获取多个帧图像; 图像保存单元，用于连续地保存由图像获取单元获取的帧图像; 属性信息保存单元，用于保存其中保存的帧图像的识别信息与参数相关联的属性信息; 以及用于控制这些单元的控制单元，其中，每当帧图像被保存在图像保存单元中时，控制单元将更新的属性信息保存在属性信息保存单元中。

3. 发明申请

US20080246839A1 MICROSCOPE APPARATUS AND MICROSCOPE SYSTEM 有权
标题翻译： MICROSCOPE装置和显微镜系统
公开(公告)号：US20080246839A1
公开(公告)日：2008-10-09
申请号：US12059043
申请日：2008-03-31
申请人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
发明人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
IPC分类号： H04N7/18
CPC分类号： G02B21/367
摘要： It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.
摘要翻译：可以检查观察成功或失败以及观察历史，而不等待观察完全完成，从而节省观察所需的时间和精力，并避免丢失观察珍贵样品等的机会。提供一种显微镜装置，包括图像获取单元，用于在改变多个参数的同时获取多个帧图像; 图像保存单元，用于连续地保存由图像获取单元获取的帧图像; 属性信息保存单元，用于保存其中保存的帧图像的识别信息与参数相关联的属性信息; 以及用于控制这些单元的控制单元，其中，每当帧图像被保存在图像保存单元中时，控制单元将更新的属性信息保存在属性信息保存单元中。

4. 发明申请

US20080252967A1 MICROSCOPE APPARATUS 有权
标题翻译： MICROSCOPE设备
公开(公告)号：US20080252967A1
公开(公告)日：2008-10-16
申请号：US12060994
申请日：2008-04-02
申请人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
发明人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
IPC分类号： G02B21/34
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译：在长期观察期间，可以防止图像变得不清楚。本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。

5. 发明授权

US07369308B2 Total internal reflection fluorescence microscope 有权
标题翻译：全内反射荧光显微镜
公开(公告)号：US07369308B2
公开(公告)日：2008-05-06
申请号：US11050883
申请日：2005-02-04
申请人： Hiroshi Tsuruta , Hiroshi Sasaki , Kenichi Kusaka , Akinori Araya , Tatsuo Nakata
发明人： Hiroshi Tsuruta , Hiroshi Sasaki , Kenichi Kusaka , Akinori Araya , Tatsuo Nakata
IPC分类号： G02B21/06
CPC分类号： G01N21/648 , G01N21/6458 , G02B21/0088 , G02B21/16
摘要： A total internal reflection fluorescence microscope includes a light source which generates laser light having a plurality of wavelengths, a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination, a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination, an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen, and a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
摘要翻译：全内反射荧光显微镜包括产生具有多个波长的激光的光源，通过物镜以预定的入射角将来自光源的激光照射在样本上并产生ev逝照明的聚焦光学系统观察由于ev逝照明而产生的荧光的荧光观察单元，调整照射在样本上的激光的入射角的入射角度调节器，以及控制入射角度调节器的控制器，当切换来自激光光源的激光的波长时，渐逝光的透过深度相同。

6. 发明申请

US20080151366A1 Laser microscope and control method for the same 有权
标题翻译：激光显微镜及其控制方法相同
公开(公告)号：US20080151366A1
公开(公告)日：2008-06-26
申请号：US12002508
申请日：2007-12-17
申请人： Akinori Araya , Tatsuo Nakata , Makio Ueno
发明人： Akinori Araya , Tatsuo Nakata , Makio Ueno
IPC分类号： G02B21/00
CPC分类号： G02B21/0036
摘要： It is possible to achieve a required field number and numerical aperture for microscope observation at a scanning speed equal to video-rate or higher and also to change the scanning speed with a simple configuration. The invention provides a laser microscope including a laser light source; a scanning unit configured to scan a specimen with laser light emitted from the laser light source; and an objective lens configured to focus the laser light scanned by the scanning unit on the specimen. The scanning unit is provided with an electro-optical deflecting element including an electro-optical crystal in which a refractive index gradient is induced by injecting electric current.
摘要翻译：可以以等于视频速率或更高的扫描速度实现用于显微镜观察的所需场数和数值孔径，并且还可以以简单的配置改变扫描速度。本发明提供一种包括激光光源的激光显微镜; 扫描单元，被配置为用从所述激光光源发射的激光扫描样本; 以及配置为将由扫描单元扫描的激光聚焦在样本上的物镜。扫描单元设置有电光偏转元件，其包括通过注入电流而引起折射率梯度的电光晶体。

7. 发明申请

US20050179903A1 Total internal reflection fluorescence microscope 有权
标题翻译：全内反射荧光显微镜
公开(公告)号：US20050179903A1
公开(公告)日：2005-08-18
申请号：US11050883
申请日：2005-02-04
申请人： Hiroshi Tsuruta , Hiroshi Sasaki , Kenichi Kusaka , Akinori Araya , Tatsuo Nakata
发明人： Hiroshi Tsuruta , Hiroshi Sasaki , Kenichi Kusaka , Akinori Araya , Tatsuo Nakata
IPC分类号： G01N21/55 , G01N21/64 , G02B21/00 , G02B21/16
CPC分类号： G01N21/648 , G01N21/6458 , G02B21/0088 , G02B21/16
摘要： A total internal reflection fluorescence microscope includes a light source which generates laser light having a plurality of wavelengths, a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination, a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination, an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen, and a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
摘要翻译：全内反射荧光显微镜包括产生具有多个波长的激光的光源，通过物镜以预定的入射角将来自光源的激光照射在样本上并产生ev逝照明的聚焦光学系统观察由于ev逝照明而产生的荧光的荧光观察单元，调整照射在样本上的激光的入射角的入射角度调节器，以及控制入射角度调节器的控制器，当切换来自激光光源的激光的波长时，渐逝光的透过深度相同。

8. 发明申请

US20060278530A1 Scanning laser microscope apparatus 有权
标题翻译：扫描激光显微镜装置
公开(公告)号：US20060278530A1
公开(公告)日：2006-12-14
申请号：US11447761
申请日：2006-06-06
申请人： Kunihiko Sasaki , Yasunari Matsukawa , Hiroshi Sasaki , Tatsuo Nakata
发明人： Kunihiko Sasaki , Yasunari Matsukawa , Hiroshi Sasaki , Tatsuo Nakata
IPC分类号： B01D57/02 , B01D59/42 , B01D59/50
CPC分类号： G02B21/008 , G01N21/6458
摘要： A scanning laser microscope that can quantitatively display a laser irradiation power on a display unit and that can suppress fluctuations in the laser irradiation power is provided. The scanning laser microscope includes a light source configured to emit laser light, a scanning unit configured to scan the laser light emitted from the light source on a sample, a photodetector configured to detect light from the sample, a processing unit configured to convert a signal from the photodetector into an image signal of the sample and to output the image signal, a reference light detector configured to detect part of the laser light emitted from the light source as a reference light signal, a laser light control unit configured to control the power of the laser light emitted from the light source, an irradiation-power calculation unit configured to calculate an irradiation power value at the sample on the basis of the reference light signal detected by the reference light detector, and a display unit configured to display the calculated irradiation power value at the sample.
摘要翻译：提供了一种可以在显示单元上定量显示激光照射功率并且可以抑制激光照射功率的波动的扫描激光显微镜。该扫描激光显微镜包括配置为发射激光的光源，被配置为扫描从样品上的光源发射的激光的扫描单元，被配置为检测来自样品的光的光电检测器，被配置为将信号从光电检测器到样品的图像信号并输出图像信号;参考光检测器，被配置为检测从光源发射的激光的一部分作为参考光信号;激光控制单元，被配置为控制功率照射功率计算单元，被配置为基于由参考光检测器检测的参考光信号计算样本处的照射功率值;以及显示单元，被配置为显示所计算的样品照射功率值。

9. 发明授权

US07268344B2 Scanning laser microscope apparatus 有权
标题翻译：扫描激光显微镜装置
公开(公告)号：US07268344B2
公开(公告)日：2007-09-11
申请号：US11447761
申请日：2006-06-06
申请人： Kunihiko Sasaki , Yasunari Matsukawa , Hiroshi Sasaki , Tatsuo Nakata
发明人： Kunihiko Sasaki , Yasunari Matsukawa , Hiroshi Sasaki , Tatsuo Nakata
IPC分类号： G01J1/32
CPC分类号： G02B21/008 , G01N21/6458
摘要： A scanning laser microscope that can quantitatively display a laser irradiation power on a display unit and that can suppress fluctuations in the laser irradiation power is provided. The scanning laser microscope includes a light source configured to emit laser light, a scanning unit configured to scan the laser light emitted from the light source on a sample, a photodetector configured to detect light from the sample, a processing unit configured to convert a signal from the photodetector into an image signal of the sample and to output the image signal, a reference light detector configured to detect part of the laser light emitted from the light source as a reference light signal, a laser light control unit configured to control the power of the laser light emitted from the light source, an irradiation-power calculation unit configured to calculate an irradiation power value at the sample on the basis of the reference light signal detected by the reference light detector, and a display unit configured to display the calculated irradiation power value at the sample.
摘要翻译：提供了一种可以在显示单元上定量显示激光照射功率并且可以抑制激光照射功率的波动的扫描激光显微镜。该扫描激光显微镜包括配置为发射激光的光源，被配置为扫描从样品上的光源发射的激光的扫描单元，被配置为检测来自样品的光的光电检测器，被配置为将信号从光电检测器到样品的图像信号并输出图像信号;参考光检测器，被配置为检测从光源发射的激光的一部分作为参考光信号;激光控制单元，被配置为控制功率照射功率计算单元，被配置为基于由参考光检测器检测的参考光信号计算样本处的照射功率值;以及显示单元，被配置为显示所计算的样品照射功率值。

10. 发明授权

US08730574B2 Microscope system 有权
标题翻译：显微镜系统
公开(公告)号：US08730574B2
公开(公告)日：2014-05-20
申请号：US12955428
申请日：2010-11-29
申请人： Akinori Araya , Tatsuo Nakata
发明人： Akinori Araya , Tatsuo Nakata
IPC分类号： G02B21/06 , G02B21/00
CPC分类号： G02B21/0032 , G02B21/00 , G02B21/0004 , G02B21/0092 , G02B21/06 , G02B21/088 , G02B21/14 , G02B21/26 , G02B21/30 , G02B21/33
摘要： A microscope system includes a culture unit for holding and cultivating a specimen while maintaining constant temperature and humidity; a stage for holding the culture unit; a first light-converging optical system for converging illumination light emitted from a light source on the specimen; a second light-converging optical system for converging transmitted light that has passed through the specimen; a transmitted-light pinhole provided at a position optically conjugate to the light-converging position of illumination light on the specimen to cut off part of transmitted light converged by the second light-converging optical system; a transmitted-light detector that detects transmitted light that has passed through the transmitted-light pinhole; a moving system for moving the first and second light-converging optical systems, the transmitted-light pinhole, and the transmitted-light detector and the stage relative to each other; a housing that encloses these components and cuts off external light; and a control unit.
摘要翻译：显微镜系统包括用于在保持恒温恒湿的同时保持和培养样本的培养单元; 举办文化单位的舞台; 第一聚光光学系统，用于将从光源发射的照明光聚焦在所述样本上; 用于会聚穿过所述样本的透射光的第二聚光光学系统; 透射光针孔，设置在与所述样本上的照明光的聚光位置光学共轭的位置处，以切断由所述第二聚光光学系统会聚的透射光的一部分; 透射光检测器，其检测穿过透光针孔的透射光; 用于相对于彼此移动第一和第二聚光光学系统，透射光针孔和透射光检测器和台的移动系统; 封闭这些部件并切断外部光的外壳; 和控制单元。

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