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1. 发明授权

US08144396B2 Microscope apparatus 有权
标题翻译：显微镜装置
公开(公告)号：US08144396B2
公开(公告)日：2012-03-27
申请号：US12060994
申请日：2008-04-02
申请人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
发明人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
IPC分类号： G02B21/26
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译：在长期观察期间，可以防止图像变得不清楚。本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。

2. 发明申请

US20080252967A1 MICROSCOPE APPARATUS 有权
标题翻译： MICROSCOPE设备
公开(公告)号：US20080252967A1
公开(公告)日：2008-10-16
申请号：US12060994
申请日：2008-04-02
申请人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
发明人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
IPC分类号： G02B21/34
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译：在长期观察期间，可以防止图像变得不清楚。本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。

3. 发明授权

US07876500B2 Microscope apparatus and microscope system 有权
标题翻译：显微镜仪器和显微镜系统
公开(公告)号：US07876500B2
公开(公告)日：2011-01-25
申请号：US12059043
申请日：2008-03-31
申请人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
发明人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
IPC分类号： G02B21/00
CPC分类号： G02B21/367
摘要： It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.
摘要翻译：可以检查观察成功或失败以及观察历史，而不等待观察完全完成，从而节省观察所需的时间和精力，并避免丢失观察珍贵样品等的机会。提供一种显微镜装置，包括图像获取单元，用于在改变多个参数的同时获取多个帧图像; 图像保存单元，用于连续地保存由图像获取单元获取的帧图像; 属性信息保存单元，用于保存其中保存的帧图像的识别信息与参数相关联的属性信息; 以及用于控制这些单元的控制单元，其中，每当帧图像被保存在图像保存单元中时，控制单元将更新的属性信息保存在属性信息保存单元中。

4. 发明申请

US20080246839A1 MICROSCOPE APPARATUS AND MICROSCOPE SYSTEM 有权
标题翻译： MICROSCOPE装置和显微镜系统
公开(公告)号：US20080246839A1
公开(公告)日：2008-10-09
申请号：US12059043
申请日：2008-03-31
申请人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
发明人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
IPC分类号： H04N7/18
CPC分类号： G02B21/367
摘要： It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.
摘要翻译：可以检查观察成功或失败以及观察历史，而不等待观察完全完成，从而节省观察所需的时间和精力，并避免丢失观察珍贵样品等的机会。提供一种显微镜装置，包括图像获取单元，用于在改变多个参数的同时获取多个帧图像; 图像保存单元，用于连续地保存由图像获取单元获取的帧图像; 属性信息保存单元，用于保存其中保存的帧图像的识别信息与参数相关联的属性信息; 以及用于控制这些单元的控制单元，其中，每当帧图像被保存在图像保存单元中时，控制单元将更新的属性信息保存在属性信息保存单元中。

5. 发明申请

US20080259442A1 Scanning microscope and adjusting method for the same 有权
标题翻译：扫描显微镜和调整方法相同
公开(公告)号：US20080259442A1
公开(公告)日：2008-10-23
申请号：US12079153
申请日：2008-03-25
申请人： Yasunari Matsukawa , Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori
发明人： Yasunari Matsukawa , Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori
IPC分类号： G02B21/06
CPC分类号： G02B21/008 , G02B21/365
摘要： A scanning microscope includes an objective lens for focusing illumination light onto a specimen; a scanning device for deflecting and scanning the illumination light; a pupil-projection optical system for illuminating a pupil of the objective lens with the scanned illumination light; and a total-magnification specifying unit for specifying a total magnification. An optical-system selecting unit changes at least one of the objective lens and the pupil-projection optical system such that a magnification of an optical system including the objective lens and the pupil-projection optical system is equal to or less than the total magnification specified by the total-magnification specifying unit and that the numerical aperture of the optical system is maximized. A deflection-angle determination unit determines a deflection angle of the illumination light deflected by the scanning device based on the ratio of the magnification of the optical system to the total magnification to achieve the total magnification.
摘要翻译：扫描显微镜包括用于将照明光聚焦到样本上的物镜; 用于偏转和扫描照明光的扫描装置; 用于利用扫描的照明光照射物镜的光瞳的光瞳投影光学系统; 以及用于指定总放大率的总放大率指定单元。光学系统选择单元改变物镜和瞳孔投影光学系统中的至少一个，使得包括物镜和瞳孔投影光学系统的光学系统的放大倍数等于或小于指定的总倍率通过总倍率指定单元，并且光学系统的数值孔径最大化。偏转角确定单元基于光学系统的倍率与总放大率的比率确定由扫描装置偏转的照明光的偏转角，以实现总放大率。

6. 发明授权

US07773297B2 Scanning microscope and adjusting method for the same 有权
标题翻译：扫描显微镜和调整方法相同
公开(公告)号：US07773297B2
公开(公告)日：2010-08-10
申请号：US12079153
申请日：2008-03-25
申请人： Yasunari Matsukawa , Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori
发明人： Yasunari Matsukawa , Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori
IPC分类号： G02B21/06
CPC分类号： G02B21/008 , G02B21/365
摘要： A scanning microscope includes an objective lens for focusing illumination light onto a specimen; a scanning device for deflecting and scanning the illumination light; a pupil-projection optical system for illuminating a pupil of the objective lens with the scanned illumination light; and a total-magnification specifying unit for specifying a total magnification. An optical-system selecting unit changes at least one of the objective lens and the pupil-projection optical system such that a magnification of an optical system including the objective lens and the pupil-projection optical system is equal to or less than the total magnification specified by the total-magnification specifying unit and that the numerical aperture of the optical system is maximized. A deflection-angle determination unit determines a deflection angle of the illumination light deflected by the scanning device based on the ratio of the magnification of the optical system to the total magnification to achieve the total magnification.
摘要翻译：扫描显微镜包括用于将照明光聚焦到样本上的物镜; 用于偏转和扫描照明光的扫描装置; 用于利用扫描的照明光照射物镜的光瞳的光瞳投影光学系统; 以及用于指定总放大率的总放大率指定单元。光学系统选择单元改变物镜和瞳孔投影光学系统中的至少一个，使得包括物镜和瞳孔投影光学系统的光学系统的放大倍数等于或小于指定的总倍率通过总倍率指定单元，并且光学系统的数值孔径最大化。偏转角确定单元基于光学系统的倍率与总放大率的比率确定由扫描装置偏转的照明光的偏转角，以实现总放大率。

7. 发明授权

US07982170B2 Microscope system 有权
标题翻译：显微镜系统
公开(公告)号：US07982170B2
公开(公告)日：2011-07-19
申请号：US12562346
申请日：2009-09-18
申请人： Toshiyuki Hattori , Yusuke Yamashita , Yasunari Matsukawa , Masaharu Tomioka
发明人： Toshiyuki Hattori , Yusuke Yamashita , Yasunari Matsukawa , Masaharu Tomioka
IPC分类号： G01N21/64
CPC分类号： G02B21/0032 , G02B21/0076 , G02B21/0088 , G02B21/34 , G02B21/367
摘要： A microscope system comprises a microscope including a motorized stage on which is mounted a container containing a specimen and which can adjust the position of the container, a scanner scanning laser light radiated onto the specimen, an objective lens focusing the scanned laser light, an image-acquisition unit acquiring a specimen image by detecting fluorescence produced in the specimen, and a dark box containing these components; a storage unit storing the mounting position of the container on the motorized stage; an image-acquisition-position setting unit setting acquisition positions of partial images of the inside of the container, on the basis of the stored mounting position of the container; a control section controlling the microscope so as to acquire the partial images for each container on the basis of the set acquisition positions; and a map-image generating section arranging the partial images to generate a map image.
摘要翻译：显微镜系统包括显微镜，其包括电动台，其上安装有容纳样本的容器并且可以调节容器的位置，辐射到样本上的扫描仪扫描激光，聚焦扫描的激光的物镜，图像 - 检测单元通过检测在样本中产生的荧光获取标本图像，以及包含这些成分的暗盒; 存储单元，其将所述容器的安装位置存储在所述机动台上; 图像获取位置设定单元，基于所存储的所述容器的安装位置来设定所述容器内部的部分图像的获取位置; 控制部分，控制显微镜，以便根据所设置的获取位置获得每个容器的部分图像; 以及布置所述部分图像以生成地图图像的地图图像生成部。

8. 发明授权

US07696996B2 Laser scanning microscope apparatus 有权
标题翻译：激光扫描显微镜装置
公开(公告)号：US07696996B2
公开(公告)日：2010-04-13
申请号：US11294689
申请日：2005-12-05
申请人： Toshiyuki Hattori , Mitsuhiro Nakano , Yusuke Yamashita , Hiroshi Hirayama , Tatsuo Nakata
发明人： Toshiyuki Hattori , Mitsuhiro Nakano , Yusuke Yamashita , Hiroshi Hirayama , Tatsuo Nakata
IPC分类号： G06T11/20
CPC分类号： G02B21/002 , G02B21/365
摘要： A laser scanning microscope capable of quickly and accurately setting control values of control items for a microscope apparatus is provided. The control items and a time line are displayed along a vertical axis and a horizontal axis, respectively. The laser scanning microscope includes a graphical user interface configured to set the control values of the control items along the time line and a control unit configured to acquire luminance information of a specimen by irradiating the specimen with a laser beam in accordance with the control values set by the graphical user interface.
摘要翻译：提供能够快速准确地设定显微镜装置的控制项的控制值的激光扫描显微镜。控制项目和时间线分别沿垂直轴和水平轴显示。激光扫描显微镜包括图形用户界面，被配置为沿着时间线设置控制项的控制值;以及控制单元，被配置为通过根据控制值设定用激光束照射样本来获取样本的亮度信息通过图形用户界面。

9. 发明申请

US20060129353A1 Laser scanning microscope apparatus 有权
标题翻译：激光扫描显微镜装置
公开(公告)号：US20060129353A1
公开(公告)日：2006-06-15
申请号：US11294689
申请日：2005-12-05
申请人： Toshiyuki Hattori , Mitsuhiro Nakano , Yusuke Yamashita , Hiroshi Hirayama , Tatsuo Nakata
发明人： Toshiyuki Hattori , Mitsuhiro Nakano , Yusuke Yamashita , Hiroshi Hirayama , Tatsuo Nakata
IPC分类号： G21C17/00
CPC分类号： G02B21/002 , G02B21/365
摘要： A laser scanning microscope capable of quickly and accurately setting control values of control items for a microscope apparatus is provided. The control items and a time line are displayed along a vertical axis and a horizontal axis, respectively. The laser scanning microscope includes a graphical user interface configured to set the control values of the control items along the time line and a control unit configured to acquire luminance information of a specimen by irradiating the specimen with a laser beam in accordance with the control values set by the graphical user interface.
摘要翻译：提供能够快速准确地设定显微镜装置的控制项的控制值的激光扫描显微镜。控制项目和时间线分别沿垂直轴和水平轴显示。激光扫描显微镜包括图形用户界面，被配置为沿着时间线设置控制项的控制值;以及控制单元，被配置为通过根据控制值设定用激光束照射样本来获取样本的亮度信息通过图形用户界面。

10. 发明授权

US07936503B2 Laser scanning microscope 有权
标题翻译：激光扫描显微镜
公开(公告)号：US07936503B2
公开(公告)日：2011-05-03
申请号：US12069519
申请日：2008-02-11
申请人： Tetsuya Koike , Toshiyuki Hattori
发明人： Tetsuya Koike , Toshiyuki Hattori
IPC分类号： G02B21/06 , G02B21/00
CPC分类号： G02B21/16 , G02B21/0076
摘要： A laser scanning microscope separates fluorescence signals of different fluorophores in accurate unmixing by eliminating positional pixels shifts between different fluorescence images obtained through irradiation of different-wavelength laser lights. The microscope includes a laser light source capable of emitting a wavelength-changeable laser light, a correction amount determination unit that determines a correction amount for correcting an optical axis shift of the laser light, an optical axis adjusting unit that adjusts an optical axis, a scanning unit that performs two-dimensional scanning, an objective lens that focuses the laser scanning light to a specimen and fluorescence emitted from the specimen, a light detector that detects the fluorescence, and a control unit that changes the wavelength of the laser light synchronously with the scanning by the scanning unit while controlling the optical axis adjusting unit based on the correction amount determined by correction amount determination unit.
摘要翻译：激光扫描显微镜通过消除通过不同波长激光照射获得的不同荧光图像之间的位置像素偏移，在精确的混合中分离不同荧光团的荧光信号。显微镜包括能够发射波长可变的激光的激光光源，确定用于校正激光的光轴偏移的校正量的校正量确定单元，调节光轴的光轴调节单元，执行二维扫描的扫描单元，将激光扫描光聚焦到样本的物镜和从样本发出的荧光，检测荧光的光检测器，以及控制单元，其将激光的波长与在通过校正量确定单元确定的校正量来控制光轴调节单元的同时由扫描单元扫描。

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