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1. 发明申请

US20060278530A1 Scanning laser microscope apparatus 有权
标题翻译：扫描激光显微镜装置
公开(公告)号：US20060278530A1
公开(公告)日：2006-12-14
申请号：US11447761
申请日：2006-06-06
申请人： Kunihiko Sasaki , Yasunari Matsukawa , Hiroshi Sasaki , Tatsuo Nakata
发明人： Kunihiko Sasaki , Yasunari Matsukawa , Hiroshi Sasaki , Tatsuo Nakata
IPC分类号： B01D57/02 , B01D59/42 , B01D59/50
CPC分类号： G02B21/008 , G01N21/6458
摘要： A scanning laser microscope that can quantitatively display a laser irradiation power on a display unit and that can suppress fluctuations in the laser irradiation power is provided. The scanning laser microscope includes a light source configured to emit laser light, a scanning unit configured to scan the laser light emitted from the light source on a sample, a photodetector configured to detect light from the sample, a processing unit configured to convert a signal from the photodetector into an image signal of the sample and to output the image signal, a reference light detector configured to detect part of the laser light emitted from the light source as a reference light signal, a laser light control unit configured to control the power of the laser light emitted from the light source, an irradiation-power calculation unit configured to calculate an irradiation power value at the sample on the basis of the reference light signal detected by the reference light detector, and a display unit configured to display the calculated irradiation power value at the sample.
摘要翻译：提供了一种可以在显示单元上定量显示激光照射功率并且可以抑制激光照射功率的波动的扫描激光显微镜。该扫描激光显微镜包括配置为发射激光的光源，被配置为扫描从样品上的光源发射的激光的扫描单元，被配置为检测来自样品的光的光电检测器，被配置为将信号从光电检测器到样品的图像信号并输出图像信号;参考光检测器，被配置为检测从光源发射的激光的一部分作为参考光信号;激光控制单元，被配置为控制功率照射功率计算单元，被配置为基于由参考光检测器检测的参考光信号计算样本处的照射功率值;以及显示单元，被配置为显示所计算的样品照射功率值。

2. 发明授权

US07268344B2 Scanning laser microscope apparatus 有权
标题翻译：扫描激光显微镜装置
公开(公告)号：US07268344B2
公开(公告)日：2007-09-11
申请号：US11447761
申请日：2006-06-06
申请人： Kunihiko Sasaki , Yasunari Matsukawa , Hiroshi Sasaki , Tatsuo Nakata
发明人： Kunihiko Sasaki , Yasunari Matsukawa , Hiroshi Sasaki , Tatsuo Nakata
IPC分类号： G01J1/32
CPC分类号： G02B21/008 , G01N21/6458
摘要： A scanning laser microscope that can quantitatively display a laser irradiation power on a display unit and that can suppress fluctuations in the laser irradiation power is provided. The scanning laser microscope includes a light source configured to emit laser light, a scanning unit configured to scan the laser light emitted from the light source on a sample, a photodetector configured to detect light from the sample, a processing unit configured to convert a signal from the photodetector into an image signal of the sample and to output the image signal, a reference light detector configured to detect part of the laser light emitted from the light source as a reference light signal, a laser light control unit configured to control the power of the laser light emitted from the light source, an irradiation-power calculation unit configured to calculate an irradiation power value at the sample on the basis of the reference light signal detected by the reference light detector, and a display unit configured to display the calculated irradiation power value at the sample.
摘要翻译：提供了一种可以在显示单元上定量显示激光照射功率并且可以抑制激光照射功率的波动的扫描激光显微镜。该扫描激光显微镜包括配置为发射激光的光源，被配置为扫描从样品上的光源发射的激光的扫描单元，被配置为检测来自样品的光的光电检测器，被配置为将信号从光电检测器到样品的图像信号并输出图像信号;参考光检测器，被配置为检测从光源发射的激光的一部分作为参考光信号;激光控制单元，被配置为控制功率照射功率计算单元，被配置为基于由参考光检测器检测的参考光信号计算样本处的照射功率值;以及显示单元，被配置为显示所计算的样品照射功率值。

3. 发明授权

US07876500B2 Microscope apparatus and microscope system 有权
标题翻译：显微镜仪器和显微镜系统
公开(公告)号：US07876500B2
公开(公告)日：2011-01-25
申请号：US12059043
申请日：2008-03-31
申请人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
发明人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
IPC分类号： G02B21/00
CPC分类号： G02B21/367
摘要： It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.
摘要翻译：可以检查观察成功或失败以及观察历史，而不等待观察完全完成，从而节省观察所需的时间和精力，并避免丢失观察珍贵样品等的机会。提供一种显微镜装置，包括图像获取单元，用于在改变多个参数的同时获取多个帧图像; 图像保存单元，用于连续地保存由图像获取单元获取的帧图像; 属性信息保存单元，用于保存其中保存的帧图像的识别信息与参数相关联的属性信息; 以及用于控制这些单元的控制单元，其中，每当帧图像被保存在图像保存单元中时，控制单元将更新的属性信息保存在属性信息保存单元中。

4. 发明申请

US20080246839A1 MICROSCOPE APPARATUS AND MICROSCOPE SYSTEM 有权
标题翻译： MICROSCOPE装置和显微镜系统
公开(公告)号：US20080246839A1
公开(公告)日：2008-10-09
申请号：US12059043
申请日：2008-03-31
申请人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
发明人： Toshiyuki Hattori , Tatsuo Nakata , Yasunari Matsukawa , Akinori Araya , Masaharu Tomioka
IPC分类号： H04N7/18
CPC分类号： G02B21/367
摘要： It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.
摘要翻译：可以检查观察成功或失败以及观察历史，而不等待观察完全完成，从而节省观察所需的时间和精力，并避免丢失观察珍贵样品等的机会。提供一种显微镜装置，包括图像获取单元，用于在改变多个参数的同时获取多个帧图像; 图像保存单元，用于连续地保存由图像获取单元获取的帧图像; 属性信息保存单元，用于保存其中保存的帧图像的识别信息与参数相关联的属性信息; 以及用于控制这些单元的控制单元，其中，每当帧图像被保存在图像保存单元中时，控制单元将更新的属性信息保存在属性信息保存单元中。

5. 发明授权

US08144396B2 Microscope apparatus 有权
标题翻译：显微镜装置
公开(公告)号：US08144396B2
公开(公告)日：2012-03-27
申请号：US12060994
申请日：2008-04-02
申请人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
发明人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
IPC分类号： G02B21/26
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译：在长期观察期间，可以防止图像变得不清楚。本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。

6. 发明申请

US20080252967A1 MICROSCOPE APPARATUS 有权
标题翻译： MICROSCOPE设备
公开(公告)号：US20080252967A1
公开(公告)日：2008-10-16
申请号：US12060994
申请日：2008-04-02
申请人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
发明人： Masaharu Tomioka , Akinori Araya , Toshiyuki Hattori , Yasunari Matsukawa , Tatsuo Nakata
IPC分类号： G02B21/34
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译：在长期观察期间，可以防止图像变得不清楚。本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。

7. 发明授权

US07176428B2 Laser-based, multiphoton-excitation-type optical examination apparatus 有权
标题翻译：基于激光的多光子激发型光学检查装置
公开(公告)号：US07176428B2
公开(公告)日：2007-02-13
申请号：US11070191
申请日：2005-03-03
申请人： Yoshihiro Kawano , Tadashi Hirata , Tatsuo Nakata , Hiroshi Sasaki
发明人： Yoshihiro Kawano , Tadashi Hirata , Tatsuo Nakata , Hiroshi Sasaki
IPC分类号： G02B7/04 , H01J3/14 , H04B10/12
CPC分类号： G01N21/6486 , G01N21/6456
摘要： The invention provides a multiphoton-excitation-type examination apparatus that efficiently generates a multiphoton-excitation effect, that makes the measurement head compact, and that can be easily adjusted when the measurement head is replaced. The multiphoton-excitation-type examination apparatus comprises a laser light source that oscillates ultrashort pulsed laser light; an optical fiber that transmits the ultrashort pulsed laser light from the laser light source; a support member; a measurement head supported on the support member so as to be movable upwards and downwards and at an angle, and having an optical system that irradiates a specimen with the ultrashort pulsed laser light transmitted by the optical fiber that measures fluorescence or reflected light coming from the specimen; and a dispersion-compensating member, in the measurement head, that compensates for group velocity dispersion of the ultrashort pulsed laser light irradiated onto the specimen.
摘要翻译：本发明提供了一种多光子激发型检查装置，其有效地产生使得测量头紧凑，并且在更换测量头时可以容易地调节的多光子激发效果。多光子激发型检查装置包括：振荡超短脉冲激光的激光光源; 从激光光源发射超短脉冲激光的光纤; 支撑构件; 测量头支撑在支撑构件上，以便可上下移动一定角度，并且具有光学系统，该光学系统用由光纤透射的超短脉冲激光照射样本，该光纤测量荧光或来自标本; 以及在测量头中补偿照射到样本上的超短脉冲激光的组速度色散的色散补偿部件。

8. 发明授权

US08445865B2 Scanning microscope device 有权
标题翻译：扫描显微镜装置
公开(公告)号：US08445865B2
公开(公告)日：2013-05-21
申请号：US12784643
申请日：2010-05-21
申请人： Hiroshi Sasaki , Tatsuo Nakata
发明人： Hiroshi Sasaki , Tatsuo Nakata
IPC分类号： G01J1/58
CPC分类号： G02B21/0076
摘要： A scanning microscope device includes a light source that emits laser light; an X-Y galvanometer mirror that scans the laser light on a sample; an objective lens that irradiates the sample with the scanned laser light and collects fluorescence generated at an irradiated position; a non-descan-detection excitation DM that is disposed between the X-Y galvanometer mirror and the objective lens and separates the laser light and the fluorescence from each other; a fiber that receives the separated fluorescence through an entrance end thereof and emits the fluorescence from an exit end thereof that is formed in a substantially linear shape; a diffraction grating that disperses the fluorescence emitted from the exit end of the fiber in a direction orthogonal to a longitudinal direction of the exit end; and a multi-anode PMT having plural cells arrayed in the dispersing direction of the dispersed fluorescence.
摘要翻译：扫描显微镜装置包括发射激光的光源; 用于扫描样品上的激光的X-Y电流计镜; 物镜，用扫描的激光照射样品并收集在照射位置产生的荧光; 设置在X-Y电流计反射镜和物镜之间并将激光和荧光彼此分离的非除数检测激励DM; 通过其入口端接收分离的荧光并从其出口端发射形成大致线状的荧光的光纤; 衍射光栅，其在与所述出射端的长度方向正交的方向上分散从所述光纤的出射端发射的荧光; 以及具有沿分散荧光的分散方向排列的多个单元的多阳极PMT。

9. 发明授权

US07369308B2 Total internal reflection fluorescence microscope 有权
标题翻译：全内反射荧光显微镜
公开(公告)号：US07369308B2
公开(公告)日：2008-05-06
申请号：US11050883
申请日：2005-02-04
申请人： Hiroshi Tsuruta , Hiroshi Sasaki , Kenichi Kusaka , Akinori Araya , Tatsuo Nakata
发明人： Hiroshi Tsuruta , Hiroshi Sasaki , Kenichi Kusaka , Akinori Araya , Tatsuo Nakata
IPC分类号： G02B21/06
CPC分类号： G01N21/648 , G01N21/6458 , G02B21/0088 , G02B21/16
摘要： A total internal reflection fluorescence microscope includes a light source which generates laser light having a plurality of wavelengths, a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination, a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination, an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen, and a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
摘要翻译：全内反射荧光显微镜包括产生具有多个波长的激光的光源，通过物镜以预定的入射角将来自光源的激光照射在样本上并产生ev逝照明的聚焦光学系统观察由于ev逝照明而产生的荧光的荧光观察单元，调整照射在样本上的激光的入射角的入射角度调节器，以及控制入射角度调节器的控制器，当切换来自激光光源的激光的波长时，渐逝光的透过深度相同。

10. 发明申请

US20050279950A1 Multiphoton-excitation-type examination apparatus 有权
标题翻译：多光子激发型检查仪
公开(公告)号：US20050279950A1
公开(公告)日：2005-12-22
申请号：US11070191
申请日：2005-03-03
申请人： Yoshihiro Kawano , Tadashi Hirata , Tatsuo Nakata , Hiroshi Sasaki
发明人： Yoshihiro Kawano , Tadashi Hirata , Tatsuo Nakata , Hiroshi Sasaki
IPC分类号： G01N21/64 , G01T1/10
CPC分类号： G01N21/6486 , G01N21/6456
摘要： The invention provides a multiphoton-excitation-type examination apparatus that efficiently generates a multiphoton-excitation effect, that makes the measurement head compact, and that can be easily adjusted when the measurement head is replaced. The multiphoton-excitation-type examination apparatus comprises a laser light source that oscillates ultrashort pulsed laser light; an optical fiber that transmits the ultrashort pulsed laser light from the laser light source; a support member; a measurement head supported on the support member so as to be movable upwards and downwards and at an angle, and having an optical system that irradiates a specimen with the ultrashort pulsed laser light transmitted by the optical fiber that measures fluorescence or reflected light coming from the specimen; and a dispersion-compensating member, in the measurement head, that compensates for group velocity dispersion of the ultrashort pulsed laser light irradiated onto the specimen.
摘要翻译：本发明提供了一种多光子激发型检查装置，其有效地产生使得测量头紧凑，并且在更换测量头时可以容易地调节的多光子激发效果。多光子激发型检查装置包括：振荡超短脉冲激光的激光光源; 从激光光源发射超短脉冲激光的光纤; 支撑构件; 测量头支撑在支撑构件上，以便可上下移动一定角度，并且具有光学系统，该光学系统用由光纤透射的超短脉冲激光照射样本，该光纤测量荧光或来自标本; 以及在测量头中补偿照射到样本上的超短脉冲激光的组速度色散的色散补偿部件。

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