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1. 发明申请

US20120172252A1 High Density Sequence Detection Methods 审中-公开
标题翻译：高密度序列检测方法
公开(公告)号：US20120172252A1
公开(公告)日：2012-07-05
申请号：US13420998
申请日：2012-03-15
申请人： Timothy M. Woudenberg , Robert C. Jones , Kenneth J. Livak
发明人： Timothy M. Woudenberg , Robert C. Jones , Kenneth J. Livak
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： B01L3/50851 , B01L3/50853 , B01L3/50857 , B01L2200/0642 , B01L2300/044 , B01L2300/0819 , B01L2300/0829 , B01L2400/0409 , B01L2400/0487 , C12Q1/6837
摘要： A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.
摘要翻译：在包含多个多核苷酸靶标的液体样品上进行PCR的方法，其中每个多核苷酸靶标以非常低的浓度存在于样品内。该方法包括将PCR反应物施加到基底的表面以在基底的表面上产生多个反应点; 将液体样品和PCR试剂混合物加载到反应点上; 在每个反应点上形成体积小于约20纳升的密封反应室; 并放大样品。

2. 发明申请

US20100173293A1 High Density Sequence Detection Methods 审中-公开
标题翻译：高密度序列检测方法
公开(公告)号：US20100173293A1
公开(公告)日：2010-07-08
申请号：US12497398
申请日：2009-07-02
申请人： Timothy M. Woudenberg , Robert C. Jones , Kenneth J. Livak
发明人： Timothy M. Woudenberg , Robert C. Jones , Kenneth J. Livak
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： B01L3/50851 , B01L3/50853 , B01L3/50857 , B01L2200/0642 , B01L2300/044 , B01L2300/0819 , B01L2300/0829 , B01L2400/0409 , B01L2400/0487 , C12Q1/6837
摘要： A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.
摘要翻译：在包含多个多核苷酸靶标的液体样品上进行PCR的方法，其中每个多核苷酸靶标以非常低的浓度存在于样品内。该方法包括将PCR反应物施加到基底的表面以在基底的表面上产生多个反应点; 将液体样品和PCR试剂混合物加载到反应点上; 在每个反应点上形成体积小于约20纳升的密封反应室; 并放大样品。

3. 发明申请

US20120115143A1 Universal Probe Assay Methods 有权
标题翻译：通用探针测定方法
公开(公告)号：US20120115143A1
公开(公告)日：2012-05-10
申请号：US13280214
申请日：2011-10-24
申请人： Kenneth J. Livak , Jason A. A. West , Robert C. Jones
发明人： Kenneth J. Livak , Jason A. A. West , Robert C. Jones
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6876 , C12Q2600/178 , C12Q2525/155 , C12Q2525/307 , C12Q2537/161
摘要： Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.
摘要翻译：提供了用于检测样品中靶多核苷酸的存在的试剂和方法。一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并将第一检测探针与扩增产物杂交，所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物。

4. 发明授权

US09353406B2 Universal probe assay methods 有权
标题翻译：通用探针测定方法
公开(公告)号：US09353406B2
公开(公告)日：2016-05-31
申请号：US13280214
申请日：2011-10-24
申请人： Kenneth J. Livak , Jason A. A. West , Robert C. Jones
发明人： Kenneth J. Livak , Jason A. A. West , Robert C. Jones
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6876 , C12Q2600/178 , C12Q2525/155 , C12Q2525/307 , C12Q2537/161
摘要： Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.
摘要翻译：提供了用于检测样品中靶多核苷酸的存在的试剂和方法。一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一区段和与第二探针结合序列杂交的第二区段，从而产生标记的扩增产物。

5. 发明申请

US20140186827A1 ASSAYS FOR THE DETECTION OF GENOTYPE, MUTATIONS, AND/OR ANEUPLOIDY 审中-公开
标题翻译：用于检测基因组，突变和/或异常的测定
公开(公告)号：US20140186827A1
公开(公告)日：2014-07-03
申请号：US13977629
申请日：2011-05-16
申请人： Martin Pieprzyk , Robert C. Jones , Kenneth J. Livak , Andrew May , Alain Mir , Jian Qin , Ramesh Ramakrishnan , Sandra Spurgeon , Jun Wang , Bernhard G. Zimmermann
发明人： Martin Pieprzyk , Robert C. Jones , Kenneth J. Livak , Andrew May , Alain Mir , Jian Qin , Ramesh Ramakrishnan , Sandra Spurgeon , Jun Wang , Bernhard G. Zimmermann
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
摘要： The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
摘要翻译：本发明提供用于检测基因型，突变和/或非整倍体的基于扩增的方法。这些方法具有广泛的适用性，但是特别适合于检测和定量存在于母体体液样品中的游离胎儿DNA中的靶核酸。

6. 发明申请

US20130022973A1 Multiplex Amplification for the Detection of Nucleic Acid Variations 审中-公开
标题翻译：用于检测核酸变异的多重扩增
公开(公告)号：US20130022973A1
公开(公告)日：2013-01-24
申请号：US13521400
申请日：2011-01-14
申请人： Carl L. G. Hansen , Oleh Petriv , Kevin Heyries , Kenneth J. Livak
发明人： Carl L. G. Hansen , Oleh Petriv , Kevin Heyries , Kenneth J. Livak
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： C12Q1/6851 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2537/143 , C12Q2537/165 , C12Q2563/159
摘要： Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.
摘要翻译：本文提供了试剂盒，引物和方法，用于通过将生物样品分配到离散的子样品中来检测生物样品中的相对目标来源参考源比例，其中生物样品包括目标源上的多个靶分子; 和参考源上的多个参考分子; 提供指向所述多个靶分子中的一个或多个的靶引物和指向所述多个参考分子中的一个或多个的参考引物; 用目标引物和参照引物进行数字扩增; 并用靶探针检测扩增的靶产物的存在或不存在，并用参考探针检测扩增的参考产物的存在或不存在，其中扩增的靶产物与扩增的参考产物的比率指示靶源与参考源的相对量在生物样品中。

7. 发明申请

US20110251083A1 Multiplexed Amplification of Short Nucleic Acids 审中-公开
标题翻译：短核酸的多重扩增
公开(公告)号：US20110251083A1
公开(公告)日：2011-10-13
申请号：US12762272
申请日：2010-04-16
申请人： Kai Qin LAO , Kenneth J. Livak , Neil A. Straus
发明人： Kai Qin LAO , Kenneth J. Livak , Neil A. Straus
IPC分类号： C40B30/04 , C12P19/34 , C40B40/06 , C12Q1/68
CPC分类号： C12N15/1065 , C12Q1/6851 , C12Q1/686 , C12Q2525/301 , C12Q2525/161 , C12Q2563/179 , C12Q2537/143 , C12Q2525/207
摘要： The present teachings provide methods, compositions, and kits for reverse transcribing and amplifying small nucleic acids such as micro RNAs. High levels of multiplexing are provided by the use of a zip-coded stem-loop reverse transcription primer, along with a PCR-based pre-amplification reaction that comprises a zip-coded forward primer. Detector probes in downstream decoding PCRs can take advantage of the zip-code introduced by the stem-loop reverse transcription primer. In some embodiments, further amplification is achieved by cycling the reverse transcription reaction. The present teachings also provide compositions and kits useful for performing the reverse transcription and amplification reactions described herein.
摘要翻译：本教导提供用于逆转录和扩增小核酸如微RNA的方法，组合物和试剂盒。通过使用zip编码的茎 - 环逆转录引物以及包含经翻译的正向引物的基于PCR的预扩增反应来提供高水平的复用。下游解码PCR中的检测器探针可以利用茎环逆转录引物引入的zip码。在一些实施方案中，通过循环逆转录反应来实现进一步的扩增。本教导还提供可用于进行本文所述的逆转录和扩增反应的组合物和试剂盒。

8. 发明申请

US20090239290A1 SINGLE-TUBE, READY-TO-USE ASSAY KITS, AND METHODS USING SAME 审中-公开
标题翻译：单管，即用型测试工具，以及使用相同的方法
公开(公告)号：US20090239290A1
公开(公告)日：2009-09-24
申请号：US12404674
申请日：2009-03-16
申请人： Michael W. Hunkapiller , Dennis A. Gilbert , Kenneth J. Livak , Junko Stevens , Susan K. Eddins , Michael Lucero
发明人： Michael W. Hunkapiller , Dennis A. Gilbert , Kenneth J. Livak , Junko Stevens , Susan K. Eddins , Michael Lucero
IPC分类号： C12M1/00
CPC分类号： C12Q1/6858 , G16B50/00 , C12Q2547/101
摘要： An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions.
摘要翻译：提供了包含存储在单管容器中的测定试剂和含有关于容器内容物的信息的数据存储介质的测定试剂盒。提供了使用试剂盒提供的数据来指导仪器和/或过程的方法，例如控制仪器进行扩增和/或测序反应。

9. 发明申请

US20090239233A1 SINGLE-TUBE, READY-TO-USE ASSAY KITS, AND METHODS USING SAME 审中-公开
公开(公告)号：US20090239233A1
公开(公告)日：2009-09-24
申请号：US12408870
申请日：2009-03-23
申请人： Michael W. Hunkapiller , Dennis A. Gilbert , Kenneth J. Livak , Junko Stevens , Susan K. Eddins , Michael Lucero
发明人： Michael W. Hunkapiller , Dennis A. Gilbert , Kenneth J. Livak , Junko Stevens , Susan K. Eddins , Michael Lucero
IPC分类号： C12Q1/68
CPC分类号： G01N35/00732 , G01N35/1002
摘要： An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions.

10. 发明申请

US20090071830A1 Systems and Methods for Isolating Nucleic Acids 失效
标题翻译：用于分离核酸的系统和方法
公开(公告)号：US20090071830A1
公开(公告)日：2009-03-19
申请号：US12179455
申请日：2008-07-24
申请人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
发明人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
IPC分类号： B01D57/02 , B01D61/42 , C25B9/08
CPC分类号： C12N15/101 , G01N27/44782
摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.
摘要翻译：用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间所述至少一个样品室。该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场，其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

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