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    • 1. 发明授权
    • Reversible agglutination mediators
    • 可逆凝集介体
    • US5136095A
    • 1992-08-04
    • US278870
    • 1988-12-01
    • Thomas L. TarnowskiCheng-I LinEdwin F. Ullman
    • Thomas L. TarnowskiCheng-I LinEdwin F. Ullman
    • G01N33/543
    • G01N33/54333G01N33/54313G01N33/54393Y10S436/808Y10S436/829
    • Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.
    • 公开了用于可逆地聚集悬浮在液体介质中的颗粒的化合物和方法。 该方法包括将含有颗粒的液体介质与能够在适于这种聚集的条件下聚集颗粒的聚离子聚合物组合。 此后,使颗粒与能够在足以反向聚集的条件下裂解聚离子聚合物的化学试剂接触。 任选地,在本方法中,在非特异性结合的条件下,将磁性颗粒加入到液体介质中,并且将包含聚集体的介质进行磁场梯度以将聚集体与介质分离。 本发明的化合物是聚阴离子。 当与化学试剂接触时,颗粒的聚集是可逆的,其切割聚离子聚合物内的至少一些键。
    • 2. 发明授权
    • Reversible agglutination mediators for separating cells from whole blood
    • 用于从全血分离细胞的可逆凝集介质
    • US4812401A
    • 1989-03-14
    • US51978
    • 1987-05-19
    • Thomas L. TarnowskiCheng-I LinEdwin F. Ullman
    • Thomas L. TarnowskiCheng-I LinEdwin F. Ullman
    • G01N33/48G01N33/543G01N33/553C12Q1/02
    • G01N33/54313G01N33/54333G01N33/54393
    • Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.
    • 公开了用于可逆地聚集悬浮在液体介质中的颗粒的化合物和方法。 该方法包括将含有颗粒的液体介质与能够在适于这种聚集的条件下聚集颗粒的聚离子聚合物组合。 此后,使颗粒与能够在足以反向聚集的条件下裂解聚离子聚合物的化学试剂接触。 任选地,在本方法中,在非特异性结合的条件下,将磁性颗粒加入到液体介质中,并且将包含聚集体的介质进行磁场梯度以将聚集体与介质分离。 本发明的化合物是聚阴离子。 当与化学试剂接触时,颗粒的聚集是可逆的,其切割聚离子聚合物内的至少一些键。
    • 4. 发明授权
    • Reversible agglutination mediators
    • 可逆凝集介体
    • US5370993A
    • 1994-12-06
    • US881987
    • 1992-05-12
    • Thomas L. TarnowskiCheng-I LinEdwin F. Ullman
    • Thomas L. TarnowskiCheng-I LinEdwin F. Ullman
    • G01N33/543G01N33/53
    • G01N33/54333G01N33/54313G01N33/54393Y10S436/808Y10S436/829
    • Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.
    • 公开了用于可逆地聚集悬浮在液体介质中的颗粒的化合物和方法。 该方法包括将含有颗粒的液体介质与能够在适于这种聚集的条件下聚集颗粒的聚离子聚合物组合。 此后,使颗粒与能够在足以反向聚集的条件下裂解聚离子聚合物的化学试剂接触。 任选地,在本方法中,在非特异性结合的条件下,将磁性颗粒加入到液体介质中,并且将包含聚集体的介质进行磁场梯度以将聚集体与介质分离。 本发明的化合物是聚阴离子。 当与化学试剂接触时,颗粒的聚集是可逆的,其切割聚离子聚合物内的至少一些键。